ISMt3

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
Z83858 ND Mycobacterium tuberculosis
Mycobacterium tuberculosis H37Rv
DNA section
IS Length : 2213 bp

Ends


IR Length : 40/50

IRL : TGTCGACGGCACGTGAAAACTGACCCCGGCGCGGCACCCGAATTTTGACC
IRR : TGTCAACGGCACCCGAAAACTGACCCCCTGACGGCATCTGAAAATTGACC

Insertion site


Left flankDirect repeatRight flankDR Length
CGCCCTTGTGAACGGTAAGCAGCAG5

DNA sequence

TGTCGACGGCACGTGAAAACTGACCCCGGCGCGGCACCCGAATTTTGACCCCCTGGTCGGGTGGACTGGCTCTACCCGAGCCAGGAGGACCGAAGGGAAT
GTTGACTGTGGAAGATTGGGCTGAGATTCGCCGATTGCATCGCGCGGAGGGTTTGCCGATCAAGATGATCGCCCGGGTGCTGGGGATTTCCAAGAACACG
GTGAAGTCAGCGTTGGAATCAAACCAGCAGCCGAAATATGAACGGGCACCGCAGGGTTCGATCGTTGATGCGGTTGAGCCGCGGATCCGGGAGTTGTTGC
AGGCCTATCCGACGATGCCGGCGACGGTGATCGCCGAGCGGATCGGCTGGGAGCGCTCGATTCGGGTGCTCTCGGCGCGGGTGGCCGAGCTGCGCCCGGT
GTATCTGCCGCCGGACCCGGCGTCGCGCACCACGTATGTGGCAGGCGAAATTGCCCAGTGCGACTTCTGGTTTCCGCCGATCGAGTTGCCGGTAGGGTTC
GGGCAGACCCGCACGGCCAAACAGTTGCCGGTGCTGACCATGGTGTGCGCCTATTCGCGCTGGCTGTTGGCGATGCTGCTGCCCAGCAGGTGTGCCGAGG
ACCTGTTCGCCGGCTGGTGGCGGCTGATCGAGGCGTTGGGGGCGGTGCCGCGGGTGTTGGTGTGGGATGGCGAGGGCGCGATCGGGCGCTGGCGCGGCGG
GCGGTCGGAGTTGACCACTGAGTGTCAGGCGTTCCGCGGCACGCTGGCGGCCAAGGTGCTCATCTGCCGGCCGGCCGACCCGGAGGCCAAGGGCCTCATT
GAACGGGCCCACGACTACCTGGAGCGCTCGTTTTTGCCCGGGCGGGTGTTTGCCTCGCCGGCCGATTTCAACGCCCAACTGGGCGCCTGGCTGGCGCTGG
TGAACACCCGCACCCGCCGGGCGCTGGGTTGTGCGCCCACCGATCGCATCGGCGCGGATCGGGCCGCGATGCTGAGCTTGCCGCCGGTGGCGCCGGCCAC
CGGGTGGTGCACCTCGCTGCGGCTGCCCCGGGATCACTATGTGCGCTGCGATTCCAACGACTACTCGGTGCACCCGGGTGTGATCGGGCATCGGGTGCTG
GTGCGCGCCGACCTGGAGCGGGTGCATGTGTTCTGCGACGGTGAGCTGGTCGCCGACCACGAGCGGATCTGGGCGGTCCATCAGACGGTCTCCGATCCCG
CACATGTGGAGGCGGCGAAGGTGTTGCGCCGCCGGCACTTCAGTGCAGCATCACCGGTAGTTGAGCCGCAGGTGCAGGTCCGCTCACTGAGCGACTACGA
TGACGCGCTGGGAGTCGACATCGATGGCGGGGTGGCCTGATGCCCACCACCAAAGCCACCCAGCGCCGTGATGTTTCCACCGAGATCGCTTACCTGACAA
GAGCATTGAAAGCTCCCACCCTGCGTGAGTCAGTGTCCCGGCTGGCCGATCGCGCCCGCGCCGAGAACTGGAGCCACGAAGAATACCTGGCCGCCTGCCT
GCAGCGGGAAGTGTCAGCCCGGGAGTCCCATGGTGGTGAGGGCCGCATCCGCGCCGCCCGCTTCCCGGCTCGGAAGTCGTTGGAAGAGTTCGACTTTGAG
CATGCTCGTGGCCTCAAACGCGACACCATCGCACATCTGGGCACCCTGGATTTCATCACCGCCCGCGATAACGTCGTGTTTTTGGGCCCCGCCTGGCACC
GGGAAGACTCATCTTGCGGTCGGCCTGGCGATACGCGCGTGTCAGGCCGGTCATCGGGTGCTGTTCGCCACCGCCGCCGAATGGGTAGCACGGCTCGCCG
AGGCTCACCACGCCGGGCGCATCTACGCCGAACTCACCCGGCTTTGCCGCTATCCGCTCCTGGTGGTTGACGAAGTCGGCTACATTCCGTTTGAGCCCGA
GGCCGCCAACCTCTTCTTCCAGCTGGTGTCCTCCCGGTATGAGCGGGCCAGCTTGATCGTCACGTCCAATAAGGCCTTCGGCCGGTGGGGCGAGGTTTTC
GGCGGCGACGACGTCGTTGCTGCCGCCATGATCGACCGCCTCGTCCACCATGCTGAAGTCGTCGCCCTCAAAGGCGACAGCTACCGGCTCAAAGACCGCG
ACCTCGGCCGCGTCCCACCAGCCGGAACCACCGAAGAATAACCACCAACCGCCCGGTCTAGGGGGTCAATTTTCAGATGCCGTCAGGGGGTCAGTTTTCG
GGTGCCGTTGACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1242 bp413 aa991340+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTVEDWAEIRRLHRAEGLPIKMIARVLGISKNTVKSALESNQQPKYERAPQGSIVDAVEPRIRELLQAYPTMPATVIAERIGWERSIRVLSARVAELRP
VYLPPDPASRTTYVAGEIAQCDFWFPPIELPVGFGQTRTAKQLPVLTMVCAYSRWLLAMLLPSRCAEDLFAGWWRLIEALGAVPRVLVWDGEGAIGRWRG
GRSELTTECQAFRGTLAAKVLICRPADPEAKGLIERAHDYLERSFLPGRVFASPADFNAQLGAWLALVNTRTRRALGCAPTDRIGADRAAMLSLPPVAPA
TGWCTSLRLPRDHYVRCDSNDYSVHPGVIGHRVLVRADLERVHVFCDGELVADHERIWAVHQTVSDPAHVEAAKVLRRRHFSAASPVVEPQVQVRSLSDY
DDALGVDIDGGVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
802 bp266 aa13402141+Yes
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MPTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSARESHGGEGRIRAARFPARKSLEEFDFEHARGLKRDTIAHL
GTLDFITARDNVVFLGPPGTGKTHLAVGLAIRACQAGHRVLFATAAEWVARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEAANLFFQLVSSRY
ERASLIVTSNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE

 

Blast result :
Comments
ISMt3 was found by its similarity to other IS21 family members (Nagy et al., 1997). It is located on the Mycobacterium tuberculosis H37Rv chromosome (Oliver and Harris, 1997). ISMt3 ORF2 (IstB) requires a frameshift around position 1750 (sequencing error ?). The "corrected" putative protein corresponds to the fusion between 1340-1690 and 1692-2141. ISMt3 IstA and IstB are 29 % and 40 % identical to those of IS100.
References
1] Nagy, I., Schoofs, G., Vanderleyden, J., and De Mot, R. (1997) J. Bacteriol. 179, 4635-4638.
2] Oliver, K., and Harris, D. (1997) EMBL Data Library, Rel. 52.