ISMt2

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) IS1532
Accession numberTranspositionOriginHost
BX842583 ND Mycobacterium tuberculosis
Mycobacterium tuberculosis H37Rv
DNA section
IS Length : 2645 bp

Ends


IR Length : 21/26

IRL : TGTCAGCGGTCACGTATTTCCCCAGGTTTGAGGGGTTGTCCGTCACGTAA
IRR : TGTCGACCGCCACGTAATTCCCCAGGGGTTGGCGTCACGTAATTCCCCAG

Insertion site


Left flankDirect repeatRight flankDR Length
AATACCTGTGGGGGCGCCGG0

DNA sequence

TGTCAGCGGTCACGTATTTCCCCAGGTTTGAGGGGTTGTCCGTCACGTAATTCCCCACCCCGGCTGTCACGTAATTCCCCACCATGGGCGGTGTGTCTGC
CCGGTGTGGGCCTTGCTGCAAGGTTCGCTCTGCATCCGTCGGCTGACGGATGGAAGGGCGCTGATGGCGAGGAGAAGTATCTTGATGCTCGATTTGGTCG
AGTTGTTGACCCACTGGCACGCGGGCCGCTCTCAGGTCCGGTTATCGGAGTCGCTGGGCATCGATCGCAAGACGGTCCGCAAATATACGGCGCCGGCGAT
CGCCGCCGGTATCGAGCCCGGTGGGGAGCCGCTGAGCGCCGAGCAGTGGGCGGAGTTGATCGGCGGGTGGTTTCCTGAATGATCCGGCGGCGCGGGCGTC
GACGTGGCCGCTGATCGCGCCGCATCATGATCGGATCAAGGACTGGCTGGATGCCGATGTCACGGTGGCCACGATAGCCCAACGGCTGCGTGACGACCAC
GGGGTGGCGGCGTCGGAGTCGTCGGTGAGGCGTTGGATCGCAACGCATTTCGCCGAGGAGGTGGCCCGCGAGAGAGTCACGGTGCCGCGCGGACCGGTCG
ATGCGGGTAGTGAGGCGCAGATCGATTACGGGCGGCTGGGCATGTGGTTCGACCCGGCCACCGCGCGCCGGGTCGCGGTGTGGGCGTTCGTGATGGTGCT
GGCGTTCTCCCGACACCTGTTCGTGCGTCCGGTCATCCGGATGGACCAAACCGCTTGGTGTGCTTGCCATGTCGCCGCATTCGAATTCTTCGACGGGGTG
CCGGCGCGGCTAGTGTGTGACAACCTCAGGACCGGGGTGGACAAGCCCGACCTGTACGACCCGCAGATCAACCGCTCCTACGCCGAGCTGGCCAGCCACT
ACGCCACGCTGGTCGACCCGGCCCGCGCCAGAAAACCCAAAGATAAACCCCGCGTGGAGCGGCCGATGACCTATGTGCGGGACTCGTTTTGGAAAGGCCG
CGAGTTCGATTCGCTGGCCCAGATGCAGCAGGCGGCGGTCACCTGGAGCACCGAAGTGGCCGGGCTTCGGTACTTACGTGCCTTGGAGGGCGCCCAACCC
CTGCGGATGTTCGAAGCTGTGGAGCAACAAGCGTTGATCGCATTGCCGCCCAGGGCATTTGAACTCACCAGCTGGTCGATCGGCACCGTCGGGGTGGACA
CGCACCTCAAAGTTGGCAAGGCACTCTATTCCGTGCCGTGGCGGCTGATCGGGCAACGCCTGCACGCGCGCACCGCCGGTGATGTGGTGCAGATCTTCGC
CGGCAACGATGTGGTGGCCACCCATGTGCGCCGACCCAGCGGGCGCTCCACCGACTTCTCCCACTACCCACCGGAGAAGATCGCCTTCCACATGCGCACC
CCGACCTGGTGTCGACACACCGCCGAACTGGTCGGCCCAGCCAGCCAGCAAGTGATCGCCGAATTCATGCGCGACAACGCCATCCACCACCTACGGTCGG
CCCAAGGCGTGCTCGGGCTACGCGACAAACACGGCTGCGACCGGCTGGAGGCCGCCTGCGCCCGCGCCATCGAGGTCGGCGACCCGAGCTATCGCACCAT
CAAGGGCATCCTTGTTGCCGGCACCGAACACGCCGCCAACGAGCCGACCACCAGTAGTCCGGCAAGCACCGCTGGGGGCGTTCCTGCGCGGCCCTGAACA
GTTCGGCACCGACACACCCCGTCGCCTGATCTCACAGCGGAATCCCGAACCATCCCGACACAATCCACATCGCTATTCTCAAGGAGAAGCATCCATGTCT
ATCTGTGATCCGGCGCTGCGTAATGCGCTACGTACCCTGAAACTGTCCGGCATGCTCGACACCCTCGACGCCCGCCTGGCCCAAACCCGCAACGGCGACC
TGGGGCATCTGGAATTCCTGCAAGCGTTGCGTGAAGACGAGATCGCCCGCCGCGAGTCCGCCGCCCTGACACGACGATTACGCCGCGCCAAGTTCGAAGC
CCAAGCCACCTTCGAAGACTTCGACTTCACTGCCAACCCGAAACTGCCCGGTGCGATGTTGCGCGATCTGGCCGCGCTGCGCTGGCTGGATGCCGGCGAA
TCGGTCATCCTCCACGGCCCGGTCGGCGTCGGAAAAACCCATGTAGCACAAGCACTTGTCCACGCCGTGGCCCGCCGCGGCGGCGACGTGCGCTTCGCCA
AAACCTCCCGCATGCTCTCCGACCTCGCCGGCGGGCACGCCGACCGATCCTGGGGCCAACGCATCCGCGAATACACCAAGCCGCTCGTGCTCATTCTGGA
CGACTTCGCGATGCGTGAGCACACCGCCATGCACGCTGATGACCTCTACGAGCTCATCAGCGACCGCGCCATCACTGGCAAACCGCTGATCTTGACCAGC
AACCGCGCACCGAATAACTGGTACGGCCTGTTCCCCAACCCCGTCGTCGCCGAATCACTCCTGGATCGGCTCATCAACACCAGCCACCAAATCCTCATGG
ACGGACCCAGCTACCGACCCCGCAAGAGACCCGGCCGCACCACCAGCTAGACAACGCCCACACGGGCCGCTAAGACCTACCACTGAGGCACACACCTGGG
GAATTACGTGACGCCAACCCCTGGGGAATTACGTGGCGGTCGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1053 bp410 aa6451697+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MATIAQRLRDDHGVAASESSVRRWIATHFAEEVARERVTVPRGPVDAGSEAQIDYGRLGMWFDPATARRVAVWAFVMVLAFSRHLFVRPVIRMDQTAWCA
CHVAAFEFFDGVPARLVCDNLRTGVDKPDLYDPQINRSYAELASHYATLVDPARARKPKDKPRVERPMTYVRDSFWKGREFDSLAQMQQAAVTWSTEVAG
LRYLRALEGAQPLRMFEAVEQQALIALPPRAFELTSWSIGTVGVDTHLKVGKALYSVPWRLIGQRLHARTAGDVVQIFAGNDVVATHVRRPSGRSTDFSH
YPPEKIAFHMRTPTWCRHTAELVGPASQQVIAEFMRDNAIHHLRSAQGVLGLRDKHGCDRLEAACARAIEVGDPSYRTIKGILVAGTEHAANEPTTSSPA
STAGGVPARP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17952550+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSICDPALRNALRTLKLSGMLDTLDARLAQTRNGDLGHLEFLQALREDEIARRESAALTRRLRRAKFEAQATFEDFDFTANPKLPGAMLRDLAALRWLDA
GESVILHGPVGVGKTHVAQALVHAVARRGGDVRFAKTSRMLSDLAGGHADRSWGQRIREYTKPLVLILDDFAMREHTAMHADDLYELISDRAITGKPLIL
TSNRAPNNWYGLFPNPVVAESLLDRLINTSHQILMDGPSYRPRKRPGRTTS

 

Blast result :
Comments
File updated : July 12 2012.
The transposase (ORFA) seems to be shorter than the other IS21 family transposase.
References
1] Barrell, B.G., Rajandream, M.A., and Walsh, S.V. (1996) EMBL Data Library, Rel. 48.
2] Cole,S.T., Brosch,R., Parkhill,J., Garnier,T., Churcher,C., Harris,D., Gordon,S.V., Eiglmeier,K., Gas,S., Barry,C.E. III, Tekaia,F., Badcock,K., Basham,D., Brown,D., Chillingworth,T., Connor,R., Davies,R., Devlin,K., Feltwell,T., Gentles,S., Hamlin,N., Holroyd,S., Hornsby,T., Jagels,K., Krogh,A., McLean,J., Moule,S., Murphy,L., Oliver,K., Osborne,J., Quail,M.A., Rajandream,M.A., Rogers,J., Rutter,S., Seeger,K., Skelton,J., Squares,R., Squares,S., Sulston,J.E., Taylor,K., Whitehead,S. and Barrell,B.G. (1998) Nature 393 (6685), 537-544