ISMt1
- Family IS5
- Group IS427
Isoform Synonym(s) ISTUB1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X65618 | ND | Mycobacterium tuberculosis | Mycobacterium tuberculosis H37Rv Mycobacterium tuberculosis Johnston Mycobacterium bovis BCG |
DNA section
IS Length : 969 bp
Ends
IR Length : 16/17
IRL : GGCGTGTCTCCCAAATTTTTAGGTACTGGCCAGCGAGGATTGGCCGGTGA
IRR : GGCGTGTCTCCCAATTTCGGAGTTCCCACTCGGGCGTGGATGACGGCGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCTGCAGTA | CTAG | CCGAGACCGG | 4 |
DNA sequence
GGCGTGTCTCCCAAATTTTTAGGTACTGGCCAGCGAGGATTGGCCGGTGACGCGAGTGGGTGTGATTTCGGACGAGTTCTGGGCCGTGGTCGAGCCGTTG
ATGCCGTCGCATGAGGGCAAGCCCGGCAGACGGTTTAGCGATCACCGGCTTATCCTGGAAGGGATCGCGTGGCGGTTCCGTACGGGAAGTCCGTGGCGGG
ACCTGCCCGCTGAGTTCGGGCCGTGGCAAACGGTGTGGAAGCGCCATCACCGTTGGTCGCTGGATGGTACCTGCGACGAGGTGTTCGCCCACGTTGCCGC
GGTGTTCGGGGTGGACGCTGAGGTGGCCGAGGATATCGAGAAGCTGCTGTCGGTGGATTCCACGAACGTGCGGGCACACCAGCATTCGGCGGGCGCTGCT
CGGACACGCTCGCCACAGGGGGCACTGTCGGATTACAAGAAATCCGCCGATGAACCCGACGATCATGCGATCGGCCGCTCGCGCGGCGGGCTGACCACCA
AGATCCATGCCCTGACCGATCAGCGCGAAGCCCCGGTGCGGATCCGGTTGACCGCAGGCCAGGCCGGCGACAACCCGCAACTGCTGCCCCTGCTCGACGA
CTATCGCCATGCCAGCACCGAATACGCCCTGGGCAGCACGGATTTCCGCTTACTCGCCGACAAGGCCTACTCACACCCAAGTACCCGTGCCGCATTACGG
TCTAAGAAGATCAAGCACACCATCCCCGAACGCCACGATCAGATCGACCGGCGCAAGGCCAAGGGGTCTGCCGGCGGGCGGCCACCAGCATTCGACGCCG
CGCTCTACGGGCTACGCAACACCGTCGAACGCGGCTTCCATCGACTCAAGCAGTGGCGCGGCATCGCAACCCGCTACGACAAATACGCCCTGACCTACCT
CGGCGGCGTCCTGCTGGCCTGCGCCGTCATCCACGCCCGAGTGGGAACTCCGAAATTGGGAGACACGCC
ATGCCGTCGCATGAGGGCAAGCCCGGCAGACGGTTTAGCGATCACCGGCTTATCCTGGAAGGGATCGCGTGGCGGTTCCGTACGGGAAGTCCGTGGCGGG
ACCTGCCCGCTGAGTTCGGGCCGTGGCAAACGGTGTGGAAGCGCCATCACCGTTGGTCGCTGGATGGTACCTGCGACGAGGTGTTCGCCCACGTTGCCGC
GGTGTTCGGGGTGGACGCTGAGGTGGCCGAGGATATCGAGAAGCTGCTGTCGGTGGATTCCACGAACGTGCGGGCACACCAGCATTCGGCGGGCGCTGCT
CGGACACGCTCGCCACAGGGGGCACTGTCGGATTACAAGAAATCCGCCGATGAACCCGACGATCATGCGATCGGCCGCTCGCGCGGCGGGCTGACCACCA
AGATCCATGCCCTGACCGATCAGCGCGAAGCCCCGGTGCGGATCCGGTTGACCGCAGGCCAGGCCGGCGACAACCCGCAACTGCTGCCCCTGCTCGACGA
CTATCGCCATGCCAGCACCGAATACGCCCTGGGCAGCACGGATTTCCGCTTACTCGCCGACAAGGCCTACTCACACCCAAGTACCCGTGCCGCATTACGG
TCTAAGAAGATCAAGCACACCATCCCCGAACGCCACGATCAGATCGACCGGCGCAAGGCCAAGGGGTCTGCCGGCGGGCGGCCACCAGCATTCGACGCCG
CGCTCTACGGGCTACGCAACACCGTCGAACGCGGCTTCCATCGACTCAAGCAGTGGCGCGGCATCGCAACCCGCTACGACAAATACGCCCTGACCTACCT
CGGCGGCGTCCTGCTGGCCTGCGCCGTCATCCACGCCCGAGTGGGAACTCCGAAATTGGGAGACACGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 923 bp | 307 aa | 47 | 969 | + | No |
Chemistry : DDE
ORF sequence :
MTRVGVISDEFWAVVEPLMPSHEGKPGRRFSDHRLILEGIAWRFRTGSPWRDLPAEFGPWQTVWKRHHRWSLDGTCDEVFAHVAAVFGVDAEVAEDIEKL
LSVDSTNVRAHQHSAGAARTRSPQGALSDYKKSADEPDDHAIGRSRGGLTTKIHALTDQREAPVRIRLTAGQAGDNPQLLPLLDDYRHASTEYALGSTDF
RLLADKAYSHPSTRAALRSKKIKHTIPERHDQIDRRKAKGSAGGRPPAFDAALYGLRNTVERGFHRLKQWRGIATRYDKYALTYLGGVLLACAVIHARVG
TPKLGDT
LSVDSTNVRAHQHSAGAARTRSPQGALSDYKKSADEPDDHAIGRSRGGLTTKIHALTDQREAPVRIRLTAGQAGDNPQLLPLLDDYRHASTEYALGSTDF
RLLADKAYSHPSTRAALRSKKIKHTIPERHDQIDRRKAKGSAGGRPPAFDAALYGLRNTVERGFHRLKQWRGIATRYDKYALTYLGGVLLACAVIHARVG
TPKLGDT
Blast result :
Comments
ISMt1 is specific for species of the Mycobacterium tuberculosis complex, including the vaccine strain Mycobacterium bovis BCG. Note that there is no stop codon at the end of the element. However, the (preferred ?) target of ISMt1 is 5'-CTAG-3', where the "TAG" is in frame with the ORF. When compared to the other IS5 family members, the ISMt1 putative transposase requires the introduction of several important gaps within the other proteins (around positions 90, 130 and 200) in other to maximize the similarity. Also, the length of the DR (4 bp) could very well be 2 !
References
1] Mariani, F., Piccolella, E., Colizzi, V., Rappuoli, R., and Gross, R. (1993) J. Gen. Microbiol. 139, 1767-1772.