ISMav1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF125999 ND Mycobacterium avium
Mycobacterium avium subsp. silvaticum
DNA section
IS Length : 2536 bp

Ends


IR Length : 41/52

IRL : TGTCCATGGCCATGAGAAAGTCCCCATCTGTGGCCAGTAGGAAGTCCCCA
IRR : TGTCAATGGCCAAGTAGAAGTCCCCGCTGGTGGCCAGCAGAAGTCCCCAC

Insertion site


Left flankDirect repeatRight flankDR Length
CGCGCGCGAATGCACTTAATGCGTG5

DNA sequence

TGTCCATGGCCATGAGAAAGTCCCCATCTGTGGCCAGTAGGAAGTCCCCACTGGTGGCCAGGTAGAAGTCCCCACTCCTTTGCGGGCTCGTCGTGTCTTT
TCCGGGAGCTTGAGGGCCTGGGCGGTGACGGTGATAGCGCCAACTGTCACTTGATCACCGCCCAGGGAGCTCTATTGAAGTCTGCAAGGGAGCGTATGAA
CATCATTTCGGCGTATCACCAGGTCGGGTCGTATCGCGGCGCGGCCGAGCTGTGCGGTACTACGCACAAGACGGTCAAGCGGGTCATCGAGCGGGCCGAG
GCCGGTGGCGCGCCCCCGCGGGAACCACGGCCACGCAACCTCGACGCGTTCACCGATCTAGTCGCCACCCGAGTCGAGAAATCACACGGCAAGATGTCGG
CGAAGCGGATGCTGCCGATCGCCCGAGCTGCCGGGTATCAGGGCTCGGCCCGTAACTTCCGCCGCCTGGTAGCCGAGCAGAAAGTATTGTGGCGCAACGC
TAACCGGCATCAACGCCGTCCGGCGGTCTGGTCACCCGGTGACTATCTGGTGATGGATTGGGCCGAAGCTGCACCGGGGTTGATGGTGTTCTGCGCGGTG
CTGGCCTATTCGCGGTGGCGGTTTGTGCGGTTCGCCGCCGACGAGAAAGCCTCGACCACGCTGGCGATGATCGCCGAAGCCCTCGAGGCGATCGGTGGGG
TTCCGGCCAAGATCCTGGCCGACCGGATGGGCTGCCTCAAAGGTGGTGTCGTCGCCAATGTTGTTGTTCCAACACCGGATTATGTGCGATTCGCGTCCCA
CTATGGCTTCGTTCCGGACTTCTGCCACGGTGCGGATCCGCAATCGAAGGGCATCGTGGAGAACCTCTGTGGCTACGCTCAGGACGACCTTGCGGTGCCG
CTGCTGACCGAAGCTGCGTTAGCCGGTGAGCAGGTCGACCTACGTGCCCTCAACGCCCAGGCGCAACTATGGTGCGCCGAGGTCAATGCCACGGTCCACT
CGGAGATCTGCGCCGTGCCCAACGATCGCTTGGTTGACGAGCGCACCGTCTTGAGGGAGCTGCCCTCGCTGCGGCCGACGATCGGCTCGGGGTCGGTGCG
CCGTAAGGTCGACGGCCTCTCGTGCATCCGTTACGGCTCAGCTCGTTACTCGGTGCCTCAGCGGCTCGTCGGTGCCACCGTGGCGGTGGTGGTCGATCAT
GGCGCCCTGATCCTGTTGGAACCTGCGACCGGTGTGATCGTGGCCGAGCACGAGCTCGTCAGCCCAGGTGAGGTGTCCATCCTCGATGAACACTACGACG
GACCCAGACCCGCACCCTCGCGTGGTCCTCGCCCGAAAAACCCAAGCAGAGAAACGATTCTGCGCATTGGGAACCGAAGCGCAAGCAAGTTCCTCGTCGG
TGCTGCTGCGATCGGCAACACCCGACTGAAATCCGAACTCGACATTCTGCTCGGCCTTGGCGCCGCCCACGGCGAACAGGCTTTGATTGACGCGCTGCGC
CGGACGGTTGCGTTTCGCCGGTTCCGCGCTGCCGACGTGCGCTCGATCCTGGCCGCCGGCGCCGGCACCCCACAACCCCGCCCCGCCGGCGACGCACTCG
TGCTCGATCTGCCCACCGTCGAGACCCGCTCGTTGGAGGCCTACAAGATCAACACCACCGACGGGACGGCCTCATGACCACCGCTGCCAAGCCGGTGGCA
CCGTCCTCGGCGGCACCGCTGGCTGCTGACCTTGACGCGGCGCTGCGGCGGTTGAAGCTGGCCACGGTGCGCCGCAACGCCGCCGAGGTGTTGCAAGTCG
CCAAGACGCAACGCTGGACACCGGAGGAGATCCTGCGGACGTTGGTTGAGGCCGAGATCGCTGCCCGCGATGCCTCCAACACCGCCAACCGTCTCAAGGC
CGCAGCCTTCCCGGTCACCAAGACCCTCGACGGGTTCGACGTCACCGGATCGTCGATCACCGCAGCCACGTTCGACTACCTGTCGAGCCTGGAATGGATT
CGGGCACAACAGAACCTGGCGGTCATTGGCCCACCTGGTACGGGCAAAAGTCACCTGCTCATCGGCTGCGGGCACGCTGCCGTCCACGCCGGATTCAAAG
TCCGCTACTTCACCGCCGCCGACCTGATCGAGGTCCTCTACCGCGGCCTGGCCGACAACACCGTCGGCAAGATCATCGACACCCTGCTCCGCGCGGATCT
GGTCATCTTGGACGAGATCGGCTTCGCCCCGCTCGACGACACCGGGACTCAACTGTTGTTCCGGCTCGTGGCTGCCGGCTACGAGCGCCGCTCCCTGGCC
ATCGCCTCGCATTGGCCCTTCGAACAATGGGGGCGATTCCTGCCCGAGCACACCACCGCCGCCAGCATCCTCGATCGGCTGCTGCACCACGCCAGCATCG
TCGTCACCTCCGGCGAGTCCTACCGGATGCGCCACGCCGACCACAAGAAGGGAGCCGCCAAGAATTAGCCAACCACCCGCAGCGGAGTGGGGACTTCTGC
TGGCCACCAGCGGGGACTTCTACTTGGCCATTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1504 bp500 aa1741677+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSARERMNIISAYHQVGSYRGAAELCGTTHKTVKRVIERAEAGGAPPREPRPRNLDAFTDLVATRVEKSHGKMSAKRMLPIARAAGYQGSARNFRRLVA
EQKVLWRNANRHQRRPAVWSPGDYLVMDWAEAAPGLMVFCAVLAYSRWRFVRFAADEKASTTLAMIAEALEAIGGVPAKILADRMGCLKGGVVANVVVPT
PDYVRFASHYGFVPDFCHGADPQSKGIVENLCGYAQDDLAVPLLTEAALAGEQVDLRALNAQAQLWCAEVNATVHSEICAVPNDRLVDERTVLRELPSLR
PTIGSGSVRRKVDGLSCIRYGSARYSVPQRLVGATVAVVVDHGALILLEPATGVIVAEHELVSPGEVSILDEHYDGPRPAPSRGPRPKNPSRETILRIGN
RSASKFLVGAAAIGNTRLKSELDILLGLGAAHGEQALIDALRRTVAFRRFRAADVRSILAAGAGTPQPRPAGDALVLDLPTVETRSLEAYKINTTDGTAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
795 bp264 aa16742468+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTTAAKPVAPSSAAPLAADLDAALRRLKLATVRRNAAEVLQVAKTQRWTPEEILRTLVEADAEIAARDASNTANRLKAAAFPVTKTLDGFDVTGSSITAA
TFDYLSSLEWIRAQQNLAVIGPDAPGTGKSHLLIGCGHAAVHAGFKVRYFTAADLIEVLYRGLADNTVGKIIDTLLRADLVILDDAEIGFAPLDDTGTQL
LFRLVAAGYERRSLAIASHWPFEQWGRFLPEHTTAASILDRLLHHADASIVVTSGESYRMRHADHKKGAAKN

 

Blast result :
Comments
Internal DR:
L1: TGGCCATGAGAAAGTCCCCATCTG (7-30)
L2: TGGCCAGTAGGAAGTCCCCACTGG (31-54)
L3: TGGCCAGGTAGAAGTCCCCACTCC (55-78)
R1: TGGCCAAGTAGAAGTCCCCGCTGG CS (2530-2507)
R2: TGGCCAGCAGAA-GTCCCCACTCC CS (2506-2484)

The elimination of a potential sequencing mistake (T175) in the ORF1 allows a N-terminal extension (MKSARER) highly homologous to the one of IS1415 (MKSSREI) with a good RBS (AGGGAG: 164-169).
References
1] Tizard, M., Bull, T., Millar, D., Doran, T., Martin, H., Sumar, N., Ford, J. and Hermon-Taylor, J. (1998) Microbiology. 144(Pt 12): p. 3413-23.

1] Berger, B. (2000) Ph.D. Thesis. University of Lausanne, Switzerland.