ISLtaq1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| D14009 | ND | Thermus aquaticus | Thermomicrobium roseum ATCC27502 Thermus aquaticus ATCC25104 |
DNA section
IS Length : 1187 bp
Ends
IR Length : 7
IRL : AAGCTTTAAGGAGCTTATTCATACGATGGTAGACAAAGACCTTAAGAGGT
IRR : AAGCTTTCGATACATGCATTATCATGGCAATTTCCTCTTCGGGAATGATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
AAGCTTTAAGGAGCTTATTCATACGATGGTAGACAAAGACCTTAAGAGGTTGAAAAAATAGTATGCAATAAAATCTAGAAAAAAGGATATCAGATATAAA
TATTCTGGTATTATCCCCTACAAGTAGACAGCTGAAAAAAGAGGACATTTGATATGATGTTCTTACGAGTCTATTTGGGAGGGGATTTTTTATGGCGAAG
AAAGGACAAACTTTTAACACCTATTCCGAAGAGCTGAAACGGGAAGTCGTTCGTTTGAAGTTGGAAGAGGGCTGGTCTTATCGTCAGCTCCGCGAACATT
TTGGAATCAAGACTGATGCCCAGATTGCGCAATGGATCAAGAAAGTTCAACGCGGCGAGTCATTAAAAGATCAACGGGGAGTGTGGAATCGCAAACATTT
TTCCAGTTTGGAAGAGGAAAACGCGTATTTGAAAGCACAGGTGGAATACCTAAAAAAGCGCAATCCAAATCTACACGGAAAGGAGTGGTCTTAAAAGCAG
AAAGGTTTCTCATTATCGATGAGCTGCGGAAAAAGCACCCACTTGCATGGCTGCTTCAAATTGGCGAAGTTTCCAAAGCTGGATACTACAAATGGCACAA
ATGCCGATCTAAAAGAGCATTACGCTTGGAAGAAGATCTTCTATTGAAAGAACATTTACTCGCCATTCATAAGATTCATCCGTACTTCGGATATAAACGA
ATGACAAGGGCATTATCCAGAGAAGGAATGATGGTAAACCATAAACGTGTACGGCGTCTCATGAGGGAATTGGGAATCCAATCGGTGATTCAAAAAAAAC
GTCCTTTCTACGGACGAAGAGGATCTGTGGTATTTCCGAATGTCTTAAACCGCGAATTCTGTGCCGGCCATCCGTTTCAAAAGCTCGTAACAGATATTAC
GTATGTGTGTGTGTGGGAGATGCGTTTGCTTACTTGTCTGCGGTTTTAGATTTATATAACAACGAAATTGTCGCGTGGGAATTATCGGAAAGAAACGATC
TTGAATTGGTATTAAATACTCTGAAACAGCTAGAGGAGAAGCCATTTAGCAAGAATGCTCTTCTGCATTCGGATCAAGGATTTCAATACACGACCAAATC
ATATGAGAACCAGCTCCAAGAATTCCAGATTCAAGGAAATCATTCCCGAAGAGGAAATTGCCATGATAATGCATGTATCGAAAGCTT
TATTCTGGTATTATCCCCTACAAGTAGACAGCTGAAAAAAGAGGACATTTGATATGATGTTCTTACGAGTCTATTTGGGAGGGGATTTTTTATGGCGAAG
AAAGGACAAACTTTTAACACCTATTCCGAAGAGCTGAAACGGGAAGTCGTTCGTTTGAAGTTGGAAGAGGGCTGGTCTTATCGTCAGCTCCGCGAACATT
TTGGAATCAAGACTGATGCCCAGATTGCGCAATGGATCAAGAAAGTTCAACGCGGCGAGTCATTAAAAGATCAACGGGGAGTGTGGAATCGCAAACATTT
TTCCAGTTTGGAAGAGGAAAACGCGTATTTGAAAGCACAGGTGGAATACCTAAAAAAGCGCAATCCAAATCTACACGGAAAGGAGTGGTCTTAAAAGCAG
AAAGGTTTCTCATTATCGATGAGCTGCGGAAAAAGCACCCACTTGCATGGCTGCTTCAAATTGGCGAAGTTTCCAAAGCTGGATACTACAAATGGCACAA
ATGCCGATCTAAAAGAGCATTACGCTTGGAAGAAGATCTTCTATTGAAAGAACATTTACTCGCCATTCATAAGATTCATCCGTACTTCGGATATAAACGA
ATGACAAGGGCATTATCCAGAGAAGGAATGATGGTAAACCATAAACGTGTACGGCGTCTCATGAGGGAATTGGGAATCCAATCGGTGATTCAAAAAAAAC
GTCCTTTCTACGGACGAAGAGGATCTGTGGTATTTCCGAATGTCTTAAACCGCGAATTCTGTGCCGGCCATCCGTTTCAAAAGCTCGTAACAGATATTAC
GTATGTGTGTGTGTGGGAGATGCGTTTGCTTACTTGTCTGCGGTTTTAGATTTATATAACAACGAAATTGTCGCGTGGGAATTATCGGAAAGAAACGATC
TTGAATTGGTATTAAATACTCTGAAACAGCTAGAGGAGAAGCCATTTAGCAAGAATGCTCTTCTGCATTCGGATCAAGGATTTCAATACACGACCAAATC
ATATGAGAACCAGCTCCAAGAATTCCAGATTCAAGGAAATCATTCCCGAAGAGGAAATTGCCATGATAATGCATGTATCGAAAGCTT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 192 | 494 | + | No |
Description : First part of the transposase
ORF sequence :
MAKKGQTFNTYSEELKREVVRLKLEEGWSYRQLREHFGIKTDAQIAQWIKKVQRGESLKDQRGVWNRKHFSSLEEENAYLKAQVEYLKKRNPNLHGKEWS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 871 bp | 289 aa | 317 | 1187 | + | No |
Description : Second part of the transposase
ORF sequence :
CPDCAMDQESSTRRVIKRSTGSVESQTFFQFGRGKRVFESTGGIPKKAQSKSTRKGVVLKAERFLIIDELRKKHPLAWLLQIGEVSKAGYYKWHKCRSKR
ALRLEEDLLLKEHLLAIHKIHPYFGYKRMTRALSREGMMVNHKRVRRLMRELGIQSVIQKKRPFYGRRGSVVFPNVLNREFCAGHPFQKLVTDITYVCVG
DAFAYLSAVLDLYNNEIVAWELSERNDLELVLNTLKQLEEKPFSKNALLHSDQGFQYTTKSYENQLQEFQIQGNHSRRGNCHDNACIES
ALRLEEDLLLKEHLLAIHKIHPYFGYKRMTRALSREGMMVNHKRVRRLMRELGIQSVIQKKRPFYGRRGSVVFPNVLNREFCAGHPFQKLVTDITYVCVG
DAFAYLSAVLDLYNNEIVAWELSERNDLELVLNTLKQLEEKPFSKNALLHSDQGFQYTTKSYENQLQEFQIQGNHSRRGNCHDNACIES
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 996 bp | 331 aa | 192 | 1187 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKKGQTFNTYSEELKREVVRLKLEEGWSYRQLREHFGIKTDAQIAQWIKKVQRGESLKDQRGVWNRKHFSSLEEENAYLKAQVEYLKKAQSKSTRKGVV
LKAERFLIIDELRKKHPLAWLLQIGEVSKAGYYKWHKCRSKRALRLEEDLLLKEHLLAIHKIHPYFGYKRMTRALSREGMMVNHKRVRRLMRELGIQSVI
QKKRPFYGRRGSVVFPNVLNREFCAGHPFQKLVTDITYVCVGDAFAYLSAVLDLYNNEIVAWELSERNDLELVLNTLKQLEEKPFSKNALLHSDQGFQYT
TKSYENQLQEFQIQGNHSRRGNCHDNACIES
LKAERFLIIDELRKKHPLAWLLQIGEVSKAGYYKWHKCRSKRALRLEEDLLLKEHLLAIHKIHPYFGYKRMTRALSREGMMVNHKRVRRLMRELGIQSVI
QKKRPFYGRRGSVVFPNVLNREFCAGHPFQKLVTDITYVCVGDAFAYLSAVLDLYNNEIVAWELSERNDLELVLNTLKQLEEKPFSKNALLHSDQGFQYT
TKSYENQLQEFQIQGNHSRRGNCHDNACIES
Blast result :
Comments
ISLtaq1 ORF1 is 36 % identical to that of IS1163 from Lactobacillus sake L45. Note that the reported ISLtaq1 ends (Utsumi et al., 1995) are very different than the ones from the other IS150 group members; their validity should thus be confirmed by actual transposition experiments. Moreover, based on comparison with other elements, two comments can be made: i) ISLtaq1 is probably missing its right end (no stop codon and similarity to C-terminal domain of related ORF2 missing), and ii) the fusion of two ORFs (425-904 and 907-1187) results in a putative 253 residues ORF2 sharing 50 % and 39 % identity with those of ISBt1 and IS150, respectively.
References
1] Utsumi, R., Ikeda, M., Horie, T., Yamamoto, M., Ichihara, A., Taniguchi, Y., Hashimoto, R., Tanabe, H., Obata, K., and Noda, M. (1995) Biosc. Biotech. Biochem. 59, 1707-1711.