ISLll1
- Family IS982
- Group
Isoform IS982Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HM021329 | ND | Lactococcus lactis | Lactococcus lactis subsp. lactis CV56 Lactococcus lactis subsp. lactis IL594 plasmid pIL4 Lactococcus lactis subsp. lactis ME2 plasmid pTN20 |
DNA section
IS Length : 999 bp
Ends
IR Length : 18/20
IRL : ACCCGAATTTCTAGTTAATTTCATTGGAAAATAAATAAGTCGTGCTATCC
IRR : ACCCGAATTGCTAGTTGATTATTTAGCCATGACTTGATACCCGATAGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTTTATTAT | ATTTTAAAAA | 0 | |
| TTTATTTAAT | TCTTCCATAA | 0 | |
| CATTATATTT | TTAT | TTAATTAATT | 4 |
| ACAAAAATGT | AATTGAA | GAAAATAAAT | 7 |
DNA sequence
ACCCGAATTTCTAGTTAATTTCATTGGAAAATAAATAAGTCGTGCTATCCTAATCTTAAACCACTAAGCATTAGAAAGCGCACGACTTATATGACTTATA
ATAGCACACTTCCAAAAGTTTTTGTTTATTTACTGACAACCATTGAAACGCTTTATCAAACGAGTGTTCCCCTTGAGGTTCAAAACCGAAAGAACGTCCA
TCTCGCAACATCAGATTGCTTAGTTATCGCTTGTTACCTATGGGGCGTACTGCATTTTAGTGAAACGCTTAAAGCTAAGCACCAATTGGCTCAAAGTTTA
TTTCCTAATTTCCTAGAATATTCTCGCTTTGTCCGCCGTTGTAATGCCCTCTTACCGAGTATCCAAGTCATTCGCCAAGCACTCGTCTTTAAAGAGGTTG
AAGGAATGAGTGTATCCATTATTGACAGCTTCCCCATTCCTTTGTGTCAGCCTATTCGTAATTTCAGAAGCAAAGTTCTTGGAGATTATGCAAATGTTGG
CTACAATGCTACAAAGGGACAGTACTTCTATGGATGTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCA
ATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGC
TGGAATTAGAAGGAATTGATCTAATTACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGA
GCGAGTTTTCTCTTTTTTGACAAATCTAGGAGCTGAGCGTTGTAAAAGTCGTTCGCCTCAAGGTTTTCAATTGAAATTAGAGATGATACTTTTAGCGTAT
TCTTTACTGTTAAAATCAGCTAAATCACTGGAACCAGAGACTTTAAGATATTCTATCGGGTATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
ATAGCACACTTCCAAAAGTTTTTGTTTATTTACTGACAACCATTGAAACGCTTTATCAAACGAGTGTTCCCCTTGAGGTTCAAAACCGAAAGAACGTCCA
TCTCGCAACATCAGATTGCTTAGTTATCGCTTGTTACCTATGGGGCGTACTGCATTTTAGTGAAACGCTTAAAGCTAAGCACCAATTGGCTCAAAGTTTA
TTTCCTAATTTCCTAGAATATTCTCGCTTTGTCCGCCGTTGTAATGCCCTCTTACCGAGTATCCAAGTCATTCGCCAAGCACTCGTCTTTAAAGAGGTTG
AAGGAATGAGTGTATCCATTATTGACAGCTTCCCCATTCCTTTGTGTCAGCCTATTCGTAATTTCAGAAGCAAAGTTCTTGGAGATTATGCAAATGTTGG
CTACAATGCTACAAAGGGACAGTACTTCTATGGATGTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCA
ATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGC
TGGAATTAGAAGGAATTGATCTAATTACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGA
GCGAGTTTTCTCTTTTTTGACAAATCTAGGAGCTGAGCGTTGTAAAAGTCGTTCGCCTCAAGGTTTTCAATTGAAATTAGAGATGATACTTTTAGCGTAT
TCTTTACTGTTAAAATCAGCTAAATCACTGGAACCAGAGACTTTAAGATATTCTATCGGGTATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 91 | 981 | + | No |
Chemistry : DDE
ORF sequence :
MTYNSTLPKVFVYLLTTIETLYQTSVPLEVQNRKNVHLATSDCLVIACYLWGVLHFSETLKAKHQLAQSLFPNFLEYSRFVRRCNALLPSIQVIRQALVF
KEVEGMSVSIIDSFPIPLCQPIRNFRSKVLGDYANVGYNATKGQYFYGCKCHALVSESGYVIDYTITPASMADSSMTEEVLSQFGTPTVLGDMGYLGQSL
HDRLELEGIDLITPVRKNMKQKKILFPNFSKRRKVIERVFSFLTNLGAERCKSRSPQGFQLKLEMILLAYSLLLKSAKSLEPETLRYSIGYQVMAK
KEVEGMSVSIIDSFPIPLCQPIRNFRSKVLGDYANVGYNATKGQYFYGCKCHALVSESGYVIDYTITPASMADSSMTEEVLSQFGTPTVLGDMGYLGQSL
HDRLELEGIDLITPVRKNMKQKKILFPNFSKRRKVIERVFSFLTNLGAERCKSRSPQGFQLKLEMILLAYSLLLKSAKSLEPETLRYSIGYQVMAK
Blast result :
Comments
The firt founded ISLll1 sequence (M95956) was detected by Kiel et al. (1993) through its similarities to ISBs1. It is located downstream of the abiC gene, coding for an abortive phage resistance protein (Durmaz et al., 1992). ISLll1 left end is apparently missing, and its right end has not been sequenced yet. ISLll1 partial transposase is 95.06 % identical to that of IS982. June 19 2012: The file was modified with the most closely related sequence founded at NCBI (99% DNA identity). The sequence is in fact an isoform of IS982. ISLll1 is 99% aa similar to IS982.
References
1] Durmaz, E., Higgins, D.L., and Klaenhammer, T.R. (1992) J. Bacteriol. 174, 7463-7469.
2] Kiel, J.A.K., Boels, J.M., Ten Berge, A.M., and Venema, G. (1993) DNA Sequencing Mapping 4, 1-9.
2] Kiel, J.A.K., Boels, J.M., Ten Berge, A.M., and Venema, G. (1993) DNA Sequencing Mapping 4, 1-9.