ISL5
- Family IS4
- Group ISPepr1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY040214 | ND | Lactobacillus delbrueckii | Lactobacillus delbrueckii subsp. bulgaricus |
DNA section
IS Length : 1590 bp
Ends
IR Length : 13/14
IRL : CTTAAACTTCCCACTTACAAAAGTGGGCTTGTACCTTGAAAATTCAATAG
IRR : TTTAAACTTCCCACACCAAAAAGAACTATGAAGCCTTGAAAACTCAATAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TTACTAA | NNNNNNNNNN | 7 |
DNA sequence
CTTAAACTTCCCACTTACAAAAGTGGGCTTGTACCTTGAAAATTCAATAGTTTAGCCAATAAAAAAGCACAAGGCCTTTGTTCGCTTGATATAATTAAGT
CGATCAAAACATCATCAATATCAAACGAAAGGTTGCGCTATGTCTAGTTTACCATCATTCGATACTGAAAACGAACCTAAAATTGCCCGTTCTGTTGAAA
AGTTCTTCAAGGACTACAAAGTTATGGAGCTTCTCCGTAGATGTGGGCTCCGCAAGTCCAAAGGAATCCCACTTTGGTCGATCCTTTCATACATCTTCAG
TAATGTGTTTCGAGACAGAAGCATGTACATGCAACAGAAGTATGGCAAGTGTACTGCTGGCTTCTCCAAAAACACCTACTATCGATTCATGCAGAACCCA
CATATCAACTGGCTTCGCCTCACTATTCTGCTAGCTGAGAGGATCGTCAATGGGCACCTGAAAGATCTGACTTCTGACCAACGTGCCGATTGCTTCGTCT
TTGACGATTCGCTCTACTCTAGAACTGGATACAAGAAGACTGAGCTGGCTGCCAAAGTATTCGACCACGTTTCGATGACCTACAAGAAGGGCTTTCGAAT
GATGACCATGGGTTGGACTGATGGATCCTCCTTTGTCCCAATCGCTTCATCACTCTTGTCCTCAAAGAACGATCAGAATGACCAAGAAGATCGACAAGCG
CACAATCGCGGCCAAGAGGCGGATTATGGCCCAATCAAAGGGGACCGATGTAGTTATCCAGCTGCTCGATCAAGCCTTGAAAGCAGGGCTCACTGCCAAG
TACGTCATGTTTGACACTTGGTTCTCCAATCCTCATCAGATTGTCCAAATCAGTCAACGTGGTTTGAACGTAATCGCCATGGTGAAGAAAAGCTCCAAGA
TCACATACGAGTTCGAAGAAAAGCGGATGAACGTCAAGCAGATCTTCAACGCATGCAAGAAACGTCGAGGTCGTTCAAGATATCTCCTGTCCGTCCCTGT
AAAGGTTGGAGATCCTGCCAAAGATGGCGCCCAGATTGATGCCAGAATAGTCTGCGTAAGAAATCGTTCCAACCGCAAAGACTGGATCGCTCTTATTTGC
ACGGACATGACGATCGATGAAAATGAGATCATCAGAATTTACGGCAAAAGATGGGACATCGAGGTCTTCTTCAAGACCTGCAAGAGTTTCTTGAAGCTTG
GCACTGAATACCATGGCCTGTCATATGATGCATTGACTGCCCATACAGCTTTCGTCTTTCTGCGCTACATGTTCATGTCAGTTGAGAAGCGAGATGATGA
AGATGATAGGACAATAGGCGAGCTCTTTTATTGCATGGTAGACGAGCTTGCGGACATCACTTTCTATTACTCCCTACAAACCCTGGTGGAAGCCATGTTC
GAGAGTGTGAAAGAGATCTTCCAACCGACAGAAGAGCAGATGGAAAGGTTCACCAACGCTTTCATTTCACGCCTCCCGAAGTACATGCAAGAGGCTATAT
CGCCATCATTAGCAGCTTAATTGAATATTTACTGGCTAAACTATTGAGTTTTCAAGGCTTCATAGTTCTTTTTGGTGTGGGAAGTTTAAA
CGATCAAAACATCATCAATATCAAACGAAAGGTTGCGCTATGTCTAGTTTACCATCATTCGATACTGAAAACGAACCTAAAATTGCCCGTTCTGTTGAAA
AGTTCTTCAAGGACTACAAAGTTATGGAGCTTCTCCGTAGATGTGGGCTCCGCAAGTCCAAAGGAATCCCACTTTGGTCGATCCTTTCATACATCTTCAG
TAATGTGTTTCGAGACAGAAGCATGTACATGCAACAGAAGTATGGCAAGTGTACTGCTGGCTTCTCCAAAAACACCTACTATCGATTCATGCAGAACCCA
CATATCAACTGGCTTCGCCTCACTATTCTGCTAGCTGAGAGGATCGTCAATGGGCACCTGAAAGATCTGACTTCTGACCAACGTGCCGATTGCTTCGTCT
TTGACGATTCGCTCTACTCTAGAACTGGATACAAGAAGACTGAGCTGGCTGCCAAAGTATTCGACCACGTTTCGATGACCTACAAGAAGGGCTTTCGAAT
GATGACCATGGGTTGGACTGATGGATCCTCCTTTGTCCCAATCGCTTCATCACTCTTGTCCTCAAAGAACGATCAGAATGACCAAGAAGATCGACAAGCG
CACAATCGCGGCCAAGAGGCGGATTATGGCCCAATCAAAGGGGACCGATGTAGTTATCCAGCTGCTCGATCAAGCCTTGAAAGCAGGGCTCACTGCCAAG
TACGTCATGTTTGACACTTGGTTCTCCAATCCTCATCAGATTGTCCAAATCAGTCAACGTGGTTTGAACGTAATCGCCATGGTGAAGAAAAGCTCCAAGA
TCACATACGAGTTCGAAGAAAAGCGGATGAACGTCAAGCAGATCTTCAACGCATGCAAGAAACGTCGAGGTCGTTCAAGATATCTCCTGTCCGTCCCTGT
AAAGGTTGGAGATCCTGCCAAAGATGGCGCCCAGATTGATGCCAGAATAGTCTGCGTAAGAAATCGTTCCAACCGCAAAGACTGGATCGCTCTTATTTGC
ACGGACATGACGATCGATGAAAATGAGATCATCAGAATTTACGGCAAAAGATGGGACATCGAGGTCTTCTTCAAGACCTGCAAGAGTTTCTTGAAGCTTG
GCACTGAATACCATGGCCTGTCATATGATGCATTGACTGCCCATACAGCTTTCGTCTTTCTGCGCTACATGTTCATGTCAGTTGAGAAGCGAGATGATGA
AGATGATAGGACAATAGGCGAGCTCTTTTATTGCATGGTAGACGAGCTTGCGGACATCACTTTCTATTACTCCCTACAAACCCTGGTGGAAGCCATGTTC
GAGAGTGTGAAAGAGATCTTCCAACCGACAGAAGAGCAGATGGAAAGGTTCACCAACGCTTTCATTTCACGCCTCCCGAAGTACATGCAAGAGGCTATAT
CGCCATCATTAGCAGCTTAATTGAATATTTACTGGCTAAACTATTGAGTTTTCAAGGCTTCATAGTTCTTTTTGGTGTGGGAAGTTTAAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 843 bp | 280 aa | 678 | 1520 | + | No |
Chemistry : DDE
ORF sequence :
MTKKIDKRTIAAKRRIMAQSKGTDVVIQLLDQALKAGLTAKYVMFDTWFSNPHQIVQISQRGLNVIAMVKKSSKITYEFEEKRMNVKQIFNACKKRRGRS
RYLLSVPVKVGDPAKDGAQIDARIVCVRNRSNRKDWIALICTDMTIDENEIIRIYGKRWDIEVFFKTCKSFLKLGTEYHGLSYDALTAHTAFVFLRYMFM
SVEKRDDEDDRTIGELFYCMVDELADITFYYSLQTLVEAMFESVKEIFQPTEEQMERFTNAFISRLPKYMQEAISPSLAA
RYLLSVPVKVGDPAKDGAQIDARIVCVRNRSNRKDWIALICTDMTIDENEIIRIYGKRWDIEVFFKTCKSFLKLGTEYHGLSYDALTAHTAFVFLRYMFM
SVEKRDDEDDRTIGELFYCMVDELADITFYYSLQTLVEAMFESVKEIFQPTEEQMERFTNAFISRLPKYMQEAISPSLAA
Blast result :
Comments
ISL5 is 39% aa similar to IS1634.
Additional Accession number : AY040218.
ISL4 was inserted in another IS sequence : ISL5 . This structure was inserted at the border of the lac promoter, and induced the loss of the palindromic structure of one of the operators likely involved in the binding of regulatory factors.
Additional Accession number : AY040218.
ISL4 was inserted in another IS sequence : ISL5 . This structure was inserted at the border of the lac promoter, and induced the loss of the palindromic structure of one of the operators likely involved in the binding of regulatory factors.
References
1] Lapierre,L., Mollet,B. and Germond,J.E. (2002) J. Bacteriol. 184 (4), 928-935