ISL4
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY040213 | ND | Lactobacillus delbrueckii | Lactobacillus delbrueckii subsp. bulgaricus |
DNA section
IS Length : 1658 bp
Ends
Left end : ACATACTAACCTAGTGTTGGATGATTACTCGACGGAGTAGTCGTTCAACACTTTTTCTTTTACTATGTAAAGTGGAGATGAAGCCAATGCCGTGAGCTCT
Right end : GATATTTGTCTTGATTCTCCGGGAGCTATTTCCCGATTAGGCCCTTATTTCAAAATAAAATTGCCTAAAATCGTTGATAACACTTGACTTAGAGTAGAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
ACATACTAACCTAGTGTTGGATGATTACTCGACGGAGTAGTCGTTCAACACTTTTTCTTTTACTATGTAAAGTGGAGATGAAGCCAATGCCGTGAGCTCT
TTGGACACACGATGTCGTAAATAAAACGGGCAGCTTCACCATTCAATTGAGAATCTCGTCTAAGTATGTTGACTGTTAAAGTCCTGTTGAGAAAATTAGA
TCTAATTGAATTGGGTGAAGGCAGCTCATCTCCGAGTAAAGCGGAGGTTTATTCTTATGGATTTATTGAAAACTGTTTTCGGTATTGATGTTAGCAGCCG
GAAGTCTAACGTATGCATTATGGTCAATGGCAAAAAAGTTAATGACTATGCCATCTCCAACGATATGGTAGGCTTCAATCGGCTGATAGGTGACCTTAAG
CAGGTTACTAAACCGCAGATTATCTTTGAAGCAACTGGCGTCTATTCCAGAAGACTCCAGGCGTTCTTGGACATGCACGAATTGCGCTATGTCATGACGA
ATCCGCTAGAAGCCAAAAGAAAGACAAAGGATGACCTGCACCAGAACAAGACCGATAAGCTTGATGCCTTGCATCTTGCCAAGCTGCAGTCTGAGCACCC
GCAACGGCTGGCCTACGTTCAAAACAAAGAATATCAAGAATTGATGGCCAATAACCGCATCTATGAACAGGCTTCGCACGATCTGATAACCAACAAGAAT
AGACTGCACAAAGCAGTTCAGCTCACTTTCCCAGAGATTGAACACTTACTGGCTAATCCGAGAGGGAAAAACTACTGGAGTATTGCTCTCAGATTCCCAC
ATCCAGATATCGTACTAGAAACAAAAGAAGCCGATATCATCGACTTCTTAAAAGGCTTATCTGGTATTGGTAAAAAGCGCGCTAATGACATAACGCAAAA
CCTTATTCGTTTGGCTAAAGTCGCATGCCCAGCGGTCAAAAAGAACAGTGCTCATATTCGTGGCCTCAAAATGGCTATTAACAACATTCTGAGCGCTGAA
GAAGAGTGCCAGACTGCTTTACAGGAGATGGCAAAGCTGGCTCCTAAACGGGATCTGGAGATCCTCACAAGCATTCCAGGCATCGGCGAAAACACTGCTC
TAAGAATCATTAGTGAGCTTGGGGATATCAGACGTTTTAACAACCCTAGCCAATTAAATGCCTTCGTTGGTGTTGATCCTCAAGTTTATGAATCTGGCAA
TCTCACGGCCCACCTGTCAATTTCAAAGCGTGGGACGGCTATTGGCAGAAAGGTGCTGTACTTGGCCATAAACCAAATTCAGTCGGCTAAGAAAGTGGGA
AACCCTTGTCATATTGCGGATTATTACGAGAAACGAAAACGGCCTTCTGAGACTGCAAGTCACAAGAAGGCCGCTATCGCATCGATCCATAAATTATTAC
GGACGATCTTCGCTTTAATTAAGAATGATCAATTGTATAGCTATGACGTAGCCAAACATAACCAAAGACTTTTGTCATAAAAATTGATCCAATAGATATT
ATAACAGCCTCTTTTTTGAAATTACAGAAGGTGGCTGTATTTACCATGCCCTAAATCAGATATTTGTCTTGATTCTCCGGGAGCTATTTCCCGATTAGGC
CCTTATTTCAAAATAAAATTGCCTAAAATCGTTGATAACACTTGACTTAGAGTAGAAA
TTGGACACACGATGTCGTAAATAAAACGGGCAGCTTCACCATTCAATTGAGAATCTCGTCTAAGTATGTTGACTGTTAAAGTCCTGTTGAGAAAATTAGA
TCTAATTGAATTGGGTGAAGGCAGCTCATCTCCGAGTAAAGCGGAGGTTTATTCTTATGGATTTATTGAAAACTGTTTTCGGTATTGATGTTAGCAGCCG
GAAGTCTAACGTATGCATTATGGTCAATGGCAAAAAAGTTAATGACTATGCCATCTCCAACGATATGGTAGGCTTCAATCGGCTGATAGGTGACCTTAAG
CAGGTTACTAAACCGCAGATTATCTTTGAAGCAACTGGCGTCTATTCCAGAAGACTCCAGGCGTTCTTGGACATGCACGAATTGCGCTATGTCATGACGA
ATCCGCTAGAAGCCAAAAGAAAGACAAAGGATGACCTGCACCAGAACAAGACCGATAAGCTTGATGCCTTGCATCTTGCCAAGCTGCAGTCTGAGCACCC
GCAACGGCTGGCCTACGTTCAAAACAAAGAATATCAAGAATTGATGGCCAATAACCGCATCTATGAACAGGCTTCGCACGATCTGATAACCAACAAGAAT
AGACTGCACAAAGCAGTTCAGCTCACTTTCCCAGAGATTGAACACTTACTGGCTAATCCGAGAGGGAAAAACTACTGGAGTATTGCTCTCAGATTCCCAC
ATCCAGATATCGTACTAGAAACAAAAGAAGCCGATATCATCGACTTCTTAAAAGGCTTATCTGGTATTGGTAAAAAGCGCGCTAATGACATAACGCAAAA
CCTTATTCGTTTGGCTAAAGTCGCATGCCCAGCGGTCAAAAAGAACAGTGCTCATATTCGTGGCCTCAAAATGGCTATTAACAACATTCTGAGCGCTGAA
GAAGAGTGCCAGACTGCTTTACAGGAGATGGCAAAGCTGGCTCCTAAACGGGATCTGGAGATCCTCACAAGCATTCCAGGCATCGGCGAAAACACTGCTC
TAAGAATCATTAGTGAGCTTGGGGATATCAGACGTTTTAACAACCCTAGCCAATTAAATGCCTTCGTTGGTGTTGATCCTCAAGTTTATGAATCTGGCAA
TCTCACGGCCCACCTGTCAATTTCAAAGCGTGGGACGGCTATTGGCAGAAAGGTGCTGTACTTGGCCATAAACCAAATTCAGTCGGCTAAGAAAGTGGGA
AACCCTTGTCATATTGCGGATTATTACGAGAAACGAAAACGGCCTTCTGAGACTGCAAGTCACAAGAAGGCCGCTATCGCATCGATCCATAAATTATTAC
GGACGATCTTCGCTTTAATTAAGAATGATCAATTGTATAGCTATGACGTAGCCAAACATAACCAAAGACTTTTGTCATAAAAATTGATCCAATAGATATT
ATAACAGCCTCTTTTTTGAAATTACAGAAGGTGGCTGTATTTACCATGCCCTAAATCAGATATTTGTCTTGATTCTCCGGGAGCTATTTCCCGATTAGGC
CCTTATTTCAAAATAAAATTGCCTAAAATCGTTGATAACACTTGACTTAGAGTAGAAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1161 bp | 386 aa | 320 | 1480 | + | No |
Chemistry : DEDD
ORF sequence :
MVNGKKVNDYAISNDMVGFNRLIGDLKQVTKPQIIFEATGVYSRRLQAFLDMHELRYVMTNPLEAKRKTKDDLHQNKTDKLDALHLAKLQSEHPQRLAYV
QNKEYQELMANNRIYEQASHDLITNKNRLHKAVQLTFPEIEHLLANPRGKNYWSIALRFPHPDIVLETKEADIIDFLKGLSGIGKKRANDITQNLIRLAK
VACPAVKKNSAHIRGLKMAINNILSAEEECQTALQEMAKLAPKRDLEILTSIPGIGENTALRIISELGDIRRFNNPSQLNAFVGVDPQVYESGNLTAHLS
ISKRGTAIGRKVLYLAINQIQSAKKVGNPCHIADYYEKRKRPSETASHKKAAIASIHKLLRTIFALIKNDQLYSYDVAKHNQRLLS
QNKEYQELMANNRIYEQASHDLITNKNRLHKAVQLTFPEIEHLLANPRGKNYWSIALRFPHPDIVLETKEADIIDFLKGLSGIGKKRANDITQNLIRLAK
VACPAVKKNSAHIRGLKMAINNILSAEEECQTALQEMAKLAPKRDLEILTSIPGIGENTALRIISELGDIRRFNNPSQLNAFVGVDPQVYESGNLTAHLS
ISKRGTAIGRKVLYLAINQIQSAKKVGNPCHIADYYEKRKRPSETASHKKAAIASIHKLLRTIFALIKNDQLYSYDVAKHNQRLLS
Blast result :
Comments
ISL4 is 45% aa similar to IS653. Additional Accession number : AY040218 ; AJ316615
ISL4 was inserted in another IS sequence : ISL5 . This structure was inserted at the border of the lac promoter, and induced the loss of the palindromic structure of one of the operators likely involved in the binding of regulatory factors.
ISL4 was inserted in another IS sequence : ISL5 . This structure was inserted at the border of the lac promoter, and induced the loss of the palindromic structure of one of the operators likely involved in the binding of regulatory factors.
References
1] Lapierre,L., Mollet,B. and Germond,J.E. (2002) J. Bacteriol. 184 (4), 928-935