ISL3
- Family ISL3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X79114 | Y | Lactobacillus delbrueckii | Lactobacillus delbrueckii subsp. lactis Lactobacillus delbrueckii subsp. bulgaricus ATCC11842 Lactobacillus delbrueckii subsp. bulgaricus ATCC21815 Lactobacillus delbrueckii subsp. bulgaricus N312 Lactobacillus delbrueckii subsp. bulgaricus N313 |
DNA section
IS Length : 1494 bp
Ends
IR Length : 27/38
IRL : GGCTCTTTGTCAAATCTTACTGATGCTTGTCAAGAAATCTTACATATGTT
IRR : GGCTCTATAATTTTTTTTACTGATGGATGTCAAGTAATACATCCGTCGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCCAAGCTA | TTTTTCGT | ATATATTATG | 8 |
| GCTAATTCAC | GATATTTT | TGATGAACAA | 8 |
| TACCAAAATC | CAAAGAAT | AAAAGATATT | 8 |
| AGAATAATGG | CATTTTAC | TGACAACGAA | 8 |
| GGCTGAGCGT | CTAATTGA | CCGCTTCTCC | 8 |
DNA sequence
GGCTCTTTGTCAAATCTTACTGATGCTTGTCAAGAAATCTTACATATGTTTGATGAAGGCACTAAACAGAACACGTCTGTTTAGTGCTTTTTTGAAGCAA
ATGATGTTCTGCTCTAGTCTGATCCTTTTCAAAAGGTGAGTGAAATTAGAATAGCCATAGGCTGTACGTTCAATTTGTTTGATCTTACGATTAACGCCCT
CTAGGCAGCCATTTGAGTACGACATTTTGGCACCATTAATGACAGCCCTCCTGTTCTTTCTAAGGGTCCGTATGGTTGTTTTCAATCGCTCACAATACTG
ACCGATATTACTGTCGAGCAGATCGTTAATTTTTTTCGTATCTTTGTCTGCTAAAGCATACACGAAATCCTGCATGAAGTCGTATGTGTTCTTCAGTGTT
TCATTGCATTTAAGGCCTTCATCAACGATTTGAGCCGGTGTATACGGTACTTTAAGACATCTGTCATAAAATTGTTTACTGCCTTCCAGATCCTTATATT
TCTTCATATACAGCTTCCAATATCGTTTTAGAAGCTTGTATTCTCTGGATTTCTTATCTAACTGTTTCATGACTTGAACTCTAAAACCGATAAAGGATCT
GTTTACCATTTGAACCATATGGAAACGGTCAATGACAAGCTCGGCGTTTGGGAAAATCTGACGCACAATATCACCGTAGTAGCAGTTGAGGTCCATACTT
ACTGTTCTAACAGTCTTTCTAACTATTTCCGGGAACTGCAAAAAGTATTTAATAATAGTGTTTTTGAAGCGATTCTCTAAAATGGCGACGATGGTATGTT
TTTCGCCATCTTGACAGATAAAGTGTAGCTTTCGCCCCACACCTCTAAATTCATCAAACGCTAAGTGTTCTGGCAAAGAATCGAATGAAGGAGTAAATTG
AGCGCTGCAGTTGTCTAAGTATCTTTGCACAGTAGTAACTGATACACCATTCTCTTCAGCAATACTGGTCATAGAATGATCTTTAGTGAGTCCGACAATG
ATCTTACGTTTAACGGCGTTAGAGATGTGGCAATACTTGTCAACGATTTGGGTAGACGGATAACTGTATAGCTCACAGTTCTTACAAAAAATACCGTGCT
TCTTGACTTTGATAATGACTGGATTGCTTTCGTCAGCTGAAATATAAGGGATATGAACGACCTTGAAGCCGTTATATATATAGTAATCAGCAGATTTACA
ATTAGGACATGTGTCGAGCTCGGCAATGGCTGTACACATGATTACTTTTCTCCCCTTGTAAGTTGTTTGAGAGCAATCTACAAAATTTAGGCAAGGGTCT
TTAACATTAAGCAAGTTTTCAATATAATTAGCAGTAGGGTTCATAGAAACCAGCTTTCTATACTTAGAAGAATTGTGGTAGAGGATTTTAAGTTACGAAT
AGCGAGGCCTATGGGCCTCTTTTTTATTTTAAGCAAAAAATCTTACCGACGGATGTATTACTTGACATCCATCAGTAAAAAAAATTATAGAGCC
ATGATGTTCTGCTCTAGTCTGATCCTTTTCAAAAGGTGAGTGAAATTAGAATAGCCATAGGCTGTACGTTCAATTTGTTTGATCTTACGATTAACGCCCT
CTAGGCAGCCATTTGAGTACGACATTTTGGCACCATTAATGACAGCCCTCCTGTTCTTTCTAAGGGTCCGTATGGTTGTTTTCAATCGCTCACAATACTG
ACCGATATTACTGTCGAGCAGATCGTTAATTTTTTTCGTATCTTTGTCTGCTAAAGCATACACGAAATCCTGCATGAAGTCGTATGTGTTCTTCAGTGTT
TCATTGCATTTAAGGCCTTCATCAACGATTTGAGCCGGTGTATACGGTACTTTAAGACATCTGTCATAAAATTGTTTACTGCCTTCCAGATCCTTATATT
TCTTCATATACAGCTTCCAATATCGTTTTAGAAGCTTGTATTCTCTGGATTTCTTATCTAACTGTTTCATGACTTGAACTCTAAAACCGATAAAGGATCT
GTTTACCATTTGAACCATATGGAAACGGTCAATGACAAGCTCGGCGTTTGGGAAAATCTGACGCACAATATCACCGTAGTAGCAGTTGAGGTCCATACTT
ACTGTTCTAACAGTCTTTCTAACTATTTCCGGGAACTGCAAAAAGTATTTAATAATAGTGTTTTTGAAGCGATTCTCTAAAATGGCGACGATGGTATGTT
TTTCGCCATCTTGACAGATAAAGTGTAGCTTTCGCCCCACACCTCTAAATTCATCAAACGCTAAGTGTTCTGGCAAAGAATCGAATGAAGGAGTAAATTG
AGCGCTGCAGTTGTCTAAGTATCTTTGCACAGTAGTAACTGATACACCATTCTCTTCAGCAATACTGGTCATAGAATGATCTTTAGTGAGTCCGACAATG
ATCTTACGTTTAACGGCGTTAGAGATGTGGCAATACTTGTCAACGATTTGGGTAGACGGATAACTGTATAGCTCACAGTTCTTACAAAAAATACCGTGCT
TCTTGACTTTGATAATGACTGGATTGCTTTCGTCAGCTGAAATATAAGGGATATGAACGACCTTGAAGCCGTTATATATATAGTAATCAGCAGATTTACA
ATTAGGACATGTGTCGAGCTCGGCAATGGCTGTACACATGATTACTTTTCTCCCCTTGTAAGTTGTTTGAGAGCAATCTACAAAATTTAGGCAAGGGTCT
TTAACATTAAGCAAGTTTTCAATATAATTAGCAGTAGGGTTCATAGAAACCAGCTTTCTATACTTAGAAGAATTGTGGTAGAGGATTTTAAGTTACGAAT
AGCGAGGCCTATGGGCCTCTTTTTTATTTTAAGCAAAAAATCTTACCGACGGATGTATTACTTGACATCCATCAGTAAAAAAAATTATAGAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1305 bp | 434 aa | 151 | 1455 | + | No |
Chemistry : Unknow
ORF sequence :
MNPTANYIENLLNVKDPCLNFVDCSQTTYKGRKVIMCTAIAELDTCPNCKSADYYIYNGFKVVHIPYISADESNPVIIKVKKHGIFCKNCELYSYPSTQI
VDKYCHISNAVKRKIIVGLTKDHSMTSIAEENGVSVTTVQRYLDNCSAQFTPSFDSLPEHLAFDEFRGVGRKLHFICQDGEKHTIVAILENRFKNTIIKY
FLQFPEIVRKTVRTVSMDLNCYYGDIVRQIFPNAELVIDRFHMVQMVNRSFIGFRVQVMKQLDKKSREYKLLKRYWKLYMKKYKDLEGSKQFYDRCLKVP
YTPAQIVDEGLKCNETLKNTYDFMQDFVYALADKDTKKINDLLDSNIGQYCERLKTTIRTLRKNRRAVINGAKMSYSNGCLEGVNRKIKQIERTAYGYSN
FTHLLKRIRLEQNIICFKKALNRRVLFSAFIKHM
VDKYCHISNAVKRKIIVGLTKDHSMTSIAEENGVSVTTVQRYLDNCSAQFTPSFDSLPEHLAFDEFRGVGRKLHFICQDGEKHTIVAILENRFKNTIIKY
FLQFPEIVRKTVRTVSMDLNCYYGDIVRQIFPNAELVIDRFHMVQMVNRSFIGFRVQVMKQLDKKSREYKLLKRYWKLYMKKYKDLEGSKQFYDRCLKVP
YTPAQIVDEGLKCNETLKNTYDFMQDFVYALADKDTKKINDLLDSNIGQYCERLKTTIRTLRKNRRAVINGAKMSYSNGCLEGVNRKIKQIERTAYGYSN
FTHLLKRIRLEQNIICFKKALNRRVLFSAFIKHM
Blast result :
Comments
ISL3 is located between the lacZ and prtP (cell wall associated protease) genes. ISL3 ORF is 34 % identical to that of IS1165. At least 1 variant (ISL3A) with one bp different at position 16 has also been partially characterized. ISL3 is not present in Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii subsp. delbrueckii (3 strains tested), Lactobacillus helveticus (6 strains tested), Lactococcus lactis (2 strains tested), Streptococcus thermophilus (8 strains tested), and Bifidobacterium bifidum (6 strains tested). Its copy number varies from 1 to more than 7. ISL3 promotes adjacent deletions (recorded in the case of the lacS-lacZ and prtP genes).
References
1] Germond, J.E., Lapierre, L., Delley, M., and Mollet, B. (1993) Fourth Symp. on Lactic Acid Bacteria, Noordwijkerhout, The Netherlands, A13, P10.
2] Germond, J.-E., Lapierre, L., and Mollet, B. (1995) Mol. Gen. Genet. 248, 407-416.
2] Germond, J.-E., Lapierre, L., and Mollet, B. (1995) Mol. Gen. Genet. 248, 407-416.