ISL2
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X77332 | Y | Lactobacillus helveticus | Lactobacillus helveticus N2 Lactobacillus helveticus LH27 |
DNA section
IS Length : 858 bp
Ends
IR Length : 16
IRL : AGAGTTACTGCGAAACATCTGCTATAATCTTAAATTATGAAATACGAAAC
IRR : AGAGTTACTGCGAAACTAGATAGCCAGTTCTTGGTTGATGATCCCGCAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAAATTAAT | AAT | GAAAAATTCG | 3 |
| ACTCAAAATC | AAT | ATATTAATGT | 3 |
| CACACAAAAT | AAT | GTAGATCGTA | 3 |
DNA sequence
AGAGTTACTGCGAAACATCTGCTATAATCTTAAATTATGAAATACGAAACAGCTAAAAATTTAAACAATACCAGATTCAAGCGTCTGATCGGCGTTGCCA
AGCCTGTCTTTGACGAAATGGTCAAAGTATTAAAAGCCGAATATCAAGTCAAGCATGCCCGAGGCGGCAGAAAGCCCAAGCTGGCAATTGAAGACCTGCT
TCTGGCTACTTTGCAGTACCTCAAGGAATACCGTACTTATGAACAAATAGCTGCCGATTACGGTGTGCATGACAGCAACCTGATCAGAAGAAGCCACTGG
GCCGAAGAGACTTTGGTTAAGCATGGCTTTAACATTGGCAAGCAAGAAATCAAGCCAGACGATGTTGTCTTAATCGATGCCACTGAAGTAAAGATTCAAC
GCCCAAAAAAAGACAAGCAGCTTATTATTCCGGCAAGAAAAAGCAGCACCGTTTTAAAAGCCCAGGCGATTACAGACACTACCGGCAGAATTATTCATTT
AGACAGCTGCCAGGCATACCGGCATGACATGCGGCTGCTGCGTGAGTCAAGGCGCAGTCTGCACCGATCCGGTTTGATTTTAGCGGATAGCGGCTATCAG
GGATTGGACAAGATTTACTTTCAGGCAAAAACACCGGTCAAATCCAGCAAGAAGAAACCGCTGACTCAGCAGGACAGAGAGCTGAATCATTTAATTTCTT
CAATTCGAATCAAGGTCGAGCATGTATTTGGCAAGGTCAAAGCATACAAAATATTTTCTACTACGTATCGCAATCATCGCCGACGTTTTAATTTAAGAAT
GAATTTAATCTGCGGGATCATCAACCAAGAACTGGCTATCTAGTTTCGCAGTAACTCT
AGCCTGTCTTTGACGAAATGGTCAAAGTATTAAAAGCCGAATATCAAGTCAAGCATGCCCGAGGCGGCAGAAAGCCCAAGCTGGCAATTGAAGACCTGCT
TCTGGCTACTTTGCAGTACCTCAAGGAATACCGTACTTATGAACAAATAGCTGCCGATTACGGTGTGCATGACAGCAACCTGATCAGAAGAAGCCACTGG
GCCGAAGAGACTTTGGTTAAGCATGGCTTTAACATTGGCAAGCAAGAAATCAAGCCAGACGATGTTGTCTTAATCGATGCCACTGAAGTAAAGATTCAAC
GCCCAAAAAAAGACAAGCAGCTTATTATTCCGGCAAGAAAAAGCAGCACCGTTTTAAAAGCCCAGGCGATTACAGACACTACCGGCAGAATTATTCATTT
AGACAGCTGCCAGGCATACCGGCATGACATGCGGCTGCTGCGTGAGTCAAGGCGCAGTCTGCACCGATCCGGTTTGATTTTAGCGGATAGCGGCTATCAG
GGATTGGACAAGATTTACTTTCAGGCAAAAACACCGGTCAAATCCAGCAAGAAGAAACCGCTGACTCAGCAGGACAGAGAGCTGAATCATTTAATTTCTT
CAATTCGAATCAAGGTCGAGCATGTATTTGGCAAGGTCAAAGCATACAAAATATTTTCTACTACGTATCGCAATCATCGCCGACGTTTTAATTTAAGAAT
GAATTTAATCTGCGGGATCATCAACCAAGAACTGGCTATCTAGTTTCGCAGTAACTCT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 807 bp | 268 aa | 37 | 843 | + | No |
Chemistry : DDE
ORF sequence :
MKYETAKNLNNTRFKRLIGVAKPVFDEMVKVLKAEYQVKHARGGRKPKLAIEDLLLATLQYLKEYRTYEQIAADYGVHDSNLIRRSHWAEETLVKHGFNI
GKQEIKPDDVVLIDATEVKIQRPKKDKQLIIPARKSSTVLKAQAITDTTGRIIHLDSCQAYRHDMRLLRESRRSLHRSGLILADSGYQGLDKIYFQAKTP
VKSSKKKPLTQQDRELNHLISSIRIKVEHVFGKVKAYKIFSTTYRNHRRRFNLRMNLICGIINQELAI
GKQEIKPDDVVLIDATEVKIQRPKKDKQLIIPARKSSTVLKAQAITDTTGRIIHLDSCQAYRHDMRLLRESRRSLHRSGLILADSGYQGLDKIYFQAKTP
VKSSKKKPLTQQDRELNHLISSIRIKVEHVFGKVKAYKIFSTTYRNHRRRFNLRMNLICGIINQELAI
Blast result :
Comments
ISL2 is associated with the appearance of Lac- phenotype (insertion into the lacL or lacM genes). ISL2 ORF is 40.1 % identical to that of IS702. ISL2 is not present in Lactobacillus acidophilus, Lactobacillus delbrueckii sp., Lactobacillus helveticus, and Streptococcus thermophilus.
References
1] Zwahlen, M.-C., and Mollet, B. (1993) Fourth Symp. on Lactic Acid Bacteria, Noordwijkerhout, The Netherlands, B9, P27.
2] Zwahlen, M.-C., and Mollet, B. (1994) Mol. Gen. Genet. 245, 334-338.
2] Zwahlen, M.-C., and Mollet, B. (1994) Mol. Gen. Genet. 245, 334-338.