ISL1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X02734 | Y | Lactobacillus casei | Lactobacillus casei C257 plasmid pLY101 Lactobacillus casei S-1 phage FSV-B Lactobacillus casei ATCC393 plasmid pLZ15 Lactobacillus casei C-9 |
DNA section
IS Length : 1257 bp
Ends
IR Length : 23/40
IRL : GGGTCTGTACTAGAATTTCGGACAGAAACATAAGAGAATTGATTTATCCG
IRR : GGGAAGTCAACACTTTTTCGGACAGTTTGCTAAGCGACTTTTTGTGAAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGAAAGATGC | ACG | TTTTACAAGC | 3 |
| CAATTCGATG | AAT | GACAGCGAAC | 3 |
DNA sequence
GGGTCTGTACTAGAATTTCGGACAGAAACATAAGAGAATTGATTTATCCGAAAGGAGTCCTCAAAATGACCTATTCACGCAATCGCTACGACCAAGATTT
TAAGAAAAATGCGGTACATTTGAGTTTTAACTCTTCCAAACCAGTTAAAATCATTGCCTCCGAACTTGGTGTTCCTGAAAGTGCCCTGTATCGTTGGCGG
AAGCTATATACCGAAGATGGAAAGCAAACACCGTTTGCTTCTTTAGAAGCTGAGAACCGTGCCTTAAAACGTGAAAATGCCGAATTAGCATTGGAGCGTG
ATATGTTAAAAAAAGCCGCCGCCTACTTTGCCAGTCTCCAGAAGTAACCCCCAGCGATAAGTATGCTTTTATGTTGAGGGAAACCTCTGTTTCTGTGGTA
AGGTGGGCGCGTTTGTTAGACGTATCAACGAGTGGTTATTATGCTTGGCGGAGAGCTTTGCCAAAGCGAAACGCCAAAGAAAGGCAATATCGTCAACGAA
TCAAACACTTTTTTGATCAAGGACAGGGCACTTACGGGCCCAAACGCATCTGCGGTTTATTGCGTAAAAATGGTAATAAAGCTTCCTATCACCGAGTTGC
CCGTCTGATGGCTGATGCCGGTCTGTATTCAACGCTACGTTGTCGTCATCCGAAGAGTTTGACCGATAGCCGCCTTGCTCGTCAAGGTGATTATCCCAAT
CTAGTGAAACACCAATCTTTTAAGCCGTTTGAAGCCTTATCAAGTGACATTACACAGACGACAACATCAGAAGGAAAAGCTTACATTTGCCAAATAAGGG
ATTTGAACAGCAACCTAGTGTTAGCTCATCAAATATCCAATCATATGGACACGGATTTAGTCTTAGCGACACTTAAACAAGCCCACCAAAGGTGGGCTTT
GCCAGAAACGTGTATTTTTCATAGTGACCGTGGTAGCCAATACACGGCCAAAGAAGTGACAAAGTTGGTCAACCAATATCATTGGCAACGTAGCTTCTCA
GCATTGGGAAAACCGGGTGATAACGCTTGGAGCGAAAGCTTCTTTGCCATCATGAAGAAAGAGCTCATTTATCAGATACAATTTAAAGATATCAATGAAC
TGAGAGCGCAAGTCTTCGCTTACATCGAGACCTTTTACAACCGCGTAAGGGTACAAGCACGCTTAGACTATCTTGCACCAGTAGCATGGCTTTCCTTGTA
TGACCAAGCTTCACAAAAAGTCGCTTAGCAAACTGTCCGAAAAAGTGTTGACTTCCC
TAAGAAAAATGCGGTACATTTGAGTTTTAACTCTTCCAAACCAGTTAAAATCATTGCCTCCGAACTTGGTGTTCCTGAAAGTGCCCTGTATCGTTGGCGG
AAGCTATATACCGAAGATGGAAAGCAAACACCGTTTGCTTCTTTAGAAGCTGAGAACCGTGCCTTAAAACGTGAAAATGCCGAATTAGCATTGGAGCGTG
ATATGTTAAAAAAAGCCGCCGCCTACTTTGCCAGTCTCCAGAAGTAACCCCCAGCGATAAGTATGCTTTTATGTTGAGGGAAACCTCTGTTTCTGTGGTA
AGGTGGGCGCGTTTGTTAGACGTATCAACGAGTGGTTATTATGCTTGGCGGAGAGCTTTGCCAAAGCGAAACGCCAAAGAAAGGCAATATCGTCAACGAA
TCAAACACTTTTTTGATCAAGGACAGGGCACTTACGGGCCCAAACGCATCTGCGGTTTATTGCGTAAAAATGGTAATAAAGCTTCCTATCACCGAGTTGC
CCGTCTGATGGCTGATGCCGGTCTGTATTCAACGCTACGTTGTCGTCATCCGAAGAGTTTGACCGATAGCCGCCTTGCTCGTCAAGGTGATTATCCCAAT
CTAGTGAAACACCAATCTTTTAAGCCGTTTGAAGCCTTATCAAGTGACATTACACAGACGACAACATCAGAAGGAAAAGCTTACATTTGCCAAATAAGGG
ATTTGAACAGCAACCTAGTGTTAGCTCATCAAATATCCAATCATATGGACACGGATTTAGTCTTAGCGACACTTAAACAAGCCCACCAAAGGTGGGCTTT
GCCAGAAACGTGTATTTTTCATAGTGACCGTGGTAGCCAATACACGGCCAAAGAAGTGACAAAGTTGGTCAACCAATATCATTGGCAACGTAGCTTCTCA
GCATTGGGAAAACCGGGTGATAACGCTTGGAGCGAAAGCTTCTTTGCCATCATGAAGAAAGAGCTCATTTATCAGATACAATTTAAAGATATCAATGAAC
TGAGAGCGCAAGTCTTCGCTTACATCGAGACCTTTTACAACCGCGTAAGGGTACAAGCACGCTTAGACTATCTTGCACCAGTAGCATGGCTTTCCTTGTA
TGACCAAGCTTCACAAAAAGTCGCTTAGCAAACTGTCCGAAAAAGTGTTGACTTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 66 | 347 | + | No |
Description : First part of the transposase
ORF sequence :
MTYSRNRYDQDFKKNAVHLSFNSSKPVKIIASELGVPESALYRWRKLYTEDGKQTPFASLEAENRALKRENAELALERDMLKKAAAYFASLQK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 929 bp | 308 aa | 300 | 1228 | + | No |
Description : Second part of the transposase
ORF sequence :
YVKKSRRLLCQSPEVTPSDKYAFMLRETSVSVVRWARLLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYHRVA
RLMADAGLYSTLRCRHPKSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLNSNLVLAHQISNHMDTDLVLATLKQAHQRWAL
PETCIFHSDRGSQYTAKEVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINELRAQVFAYIETFYNRVRVQARLDYLAPVAWLSLY
DQASQKVA
RLMADAGLYSTLRCRHPKSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLNSNLVLAHQISNHMDTDLVLATLKQAHQRWAL
PETCIFHSDRGSQYTAKEVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINELRAQVFAYIETFYNRVRVQARLDYLAPVAWLSLY
DQASQKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 66 | 1228 | + | Yes |
Chemistry : DDE
ORF sequence :
MTYSRNRYDQDFKKNAVHLSFNSSKPVKIIASELGVPESALYRWRKLYTEDGKQTPFASLEAENRALKRENAELALERDMLKKSRRLLCQSPEVTPSDKY
AFMLRETSVSVVRWARLLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYHRVARLMADAGLYSTLRCRHPKSLT
DSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLNSNLVLAHQISNHMDTDLVLATLKQAHQRWALPETCIFHSDRGSQYTAKEVTK
LVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINELRAQVFAYIETFYNRVRVQARLDYLAPVAWLSLYDQASQKVA
AFMLRETSVSVVRWARLLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYHRVARLMADAGLYSTLRCRHPKSLT
DSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLNSNLVLAHQISNHMDTDLVLATLKQAHQRWALPETCIFHSDRGSQYTAKEVTK
LVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINELRAQVFAYIETFYNRVRVQARLDYLAPVAWLSLYDQASQKVA
Blast result :
Comments
References
1] Shimizu-Kadota, M., Kiwaki, M., Hirokawa, H., and Tsuchida, N. (1985) Mol. Gen. Genet. 200, 193-198.
2] Shimizu-Kadota, M.. (1987) Appl. Environ. Microbiol. 53, 2987-2991.
3] Shimizu-Kadota, M., Flickinger, J.L., and Chassy, B.M. (1988) J. Bacteriol. 170, 4976-4978.
2] Shimizu-Kadota, M.. (1987) Appl. Environ. Microbiol. 53, 2987-2991.
3] Shimizu-Kadota, M., Flickinger, J.L., and Chassy, B.M. (1988) J. Bacteriol. 170, 4976-4978.