ISIMl1
- Family IS110
- Group
Isoform ISIMb1Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M34367 | ND | Moraxella lacunata | Moraxella lacunata ATCC17956 |
DNA section
IS Length : 969 bp
Ends
Left end : ATGTCTAAAACTTACATTGGGATTGATATCGCCAAAAACACATTTGATGCCTGCTTTATTACCCATAACACTTGGCAAAACTGTACATTCACAAATAACC
Right end : CAAAAAACAAATTATTATCGCCATCATGCGCAAATTACTCACCATCAGCTATGCCGTTATTCGCCAAAATCGCCCTTTTGACAAACGCATTCATGAATAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
ATGTCTAAAACTTACATTGGGATTGATATCGCCAAAAACACATTTGATGCCTGCTTTATTACCCATAACACTTGGCAAAACTGTACATTCACAAATAACC
AACAGGGTTTTATAGAGCTAACATTGTGGATTCAAGCACATCATTATAACACATCTACCTTACATTTGATTATTGAGGCAACGGGGGCTTATTGGGAAAA
ATTAGCTCATTGGGCAATCTCTCATCATCATAAGGTAAGTATTGTCAACCCCTTATATATTCATGCATACGCCAAAAGCCTAGGCATACGCACCAAGACA
GATAAGCAGGATGCCATTCTCTTGGCACGCTATGGTGCAAAAGAAAATCCACCTTTATGGCAGCCTAAATCAGACAACGAAATAAAACTGACTGCCCTTC
TAAAACAACGTGAACACCACAAACGGCAACTCATCAAAGAACGCACTCGACAGGAAGCTCTGTCTATTTATGTGAAATCGTATACTGATGACAACATTCG
TCATTGGAGTGACAGCATCACACAGCTCGATCATCAAATTTGGCAACTCATCAATTGCACGCCCGAGCTAAATTACCGTGCCAGCCTACTTGCCACAATA
CCTGGAATTGGCAAAAAGACACTGCCACATCTACTGGTTGTCATTGGAGATGGTTCATCATTTCAAAGTGCCAAACATCTAGCAAGCTATGCTGGGCTTG
CCCCTCGTCATCATCAATCAGGCATTAGTATTCACAAGCAGTCTTCCATTGGATTTAGTGGACAAAAAGAACTACGTTCAGCCCTTTTTATGCCCGCTGT
CATAGTCAGCTTTGGTCGATATCCCGCTTTTCAAAAGTTCGTCAAACGCATGGAACAAAAAGGCAAAACCAAAAAACAAATTATTATCGCCATCATGCGC
AAATTACTCACCATCAGCTATGCCGTTATTCGCCAAAATCGCCCTTTTGACAAACGCATTCATGAATAA
AACAGGGTTTTATAGAGCTAACATTGTGGATTCAAGCACATCATTATAACACATCTACCTTACATTTGATTATTGAGGCAACGGGGGCTTATTGGGAAAA
ATTAGCTCATTGGGCAATCTCTCATCATCATAAGGTAAGTATTGTCAACCCCTTATATATTCATGCATACGCCAAAAGCCTAGGCATACGCACCAAGACA
GATAAGCAGGATGCCATTCTCTTGGCACGCTATGGTGCAAAAGAAAATCCACCTTTATGGCAGCCTAAATCAGACAACGAAATAAAACTGACTGCCCTTC
TAAAACAACGTGAACACCACAAACGGCAACTCATCAAAGAACGCACTCGACAGGAAGCTCTGTCTATTTATGTGAAATCGTATACTGATGACAACATTCG
TCATTGGAGTGACAGCATCACACAGCTCGATCATCAAATTTGGCAACTCATCAATTGCACGCCCGAGCTAAATTACCGTGCCAGCCTACTTGCCACAATA
CCTGGAATTGGCAAAAAGACACTGCCACATCTACTGGTTGTCATTGGAGATGGTTCATCATTTCAAAGTGCCAAACATCTAGCAAGCTATGCTGGGCTTG
CCCCTCGTCATCATCAATCAGGCATTAGTATTCACAAGCAGTCTTCCATTGGATTTAGTGGACAAAAAGAACTACGTTCAGCCCTTTTTATGCCCGCTGT
CATAGTCAGCTTTGGTCGATATCCCGCTTTTCAAAAGTTCGTCAAACGCATGGAACAAAAAGGCAAAACCAAAAAACAAATTATTATCGCCATCATGCGC
AAATTACTCACCATCAGCTATGCCGTTATTCGCCAAAATCGCCCTTTTGACAAACGCATTCATGAATAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1 bp | 322 aa | 0 | 0 | + | No |
Chemistry : DEDD
ORF sequence :
MSKTYIGIDIAKNTFDACFITHNTWQNCTFTNNQQGFIELTLWIQAHHYNTSTLHLIIEATGAYWEKLAHWAISHHHKVSIVNPLYIHAYAKSLGIRTKT
DKQDAILLARYGAKENPPLWQPKSDNEIKLTALLKQREHHKRQLIKERTRQEALSIYVKSYTDDNIRHWSDSITQLDHQIWQLINCTPELNYRASLLATI
PGIGKKTLPHLLVVIGDGSSFQSAKHLASYAGLAPRHHQSGISIHKQSSIGFSGQKELRSALFMPAVIVSFGRYPAFQKFVKRMEQKGKTKKQIIIAIMR
KLLTISYAVIRQNRPFDKRIHE
DKQDAILLARYGAKENPPLWQPKSDNEIKLTALLKQREHHKRQLIKERTRQEALSIYVKSYTDDNIRHWSDSITQLDHQIWQLINCTPELNYRASLLATI
PGIGKKTLPHLLVVIGDGSSFQSAKHLASYAGLAPRHHQSGISIHKQSSIGFSGQKELRSALFMPAVIVSFGRYPAFQKFVKRMEQKGKTKKQIIIAIMR
KLLTISYAVIRQNRPFDKRIHE
Blast result :
Comments
Moraxella lacunata and Moraxella bovis are human and bovine ocular pathogens, respectively. They use a site-specific DNA inversion of a chromosomal segment to alter the expression of their 4 pilin genes, whose products mediate adherence of the bacterium to corneal tissue. Piv proteins are site-specific recombinases governing the inversion system. The Piv proteins from M. lacunata (PivML) and M. bovis (PivMB) are 98 % identical. The acronym "ISI" used here stands for IS-like Inversion system (J. Mahillon).
References
1] Marrs, C.F., Rozsa, F.W., Hackel, M., Stevens, S.P., and Glasgow, A.C. (1990) J. Bacteriol. 172, 4370-4377.
2] Lenich, A.G., and Glasgow, A.C. (1994) J. Bacteriol. 176, 4160-4164.
2] Lenich, A.G., and Glasgow, A.C. (1994) J. Bacteriol. 176, 4160-4164.