ISH8A
- Family IS4
- Group ISH8
Isoform ISH26Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF016485 | ND | Halobacterium sp. | Halobacterium sp. NRC-1 Halobacterium sp. NRC-1 plasmid pNRC100 |
DNA section
IS Length : 1402 bp
Ends
IR Length : 19
IRL : CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGGCGGCTTATCGA
IRR : CATCCGTCTTTAGTTAAGCCTCACACCTCGGTTGTGTAGTGGTAGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGGCGGCTTATCGAGGCATCTTTCGGAAGGAATGAGGAAGTCCAGTCTGTGCACGACAAGGACT
CCTCACCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTCGAAGACCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCT
CCAGATCCCTGCCTTCGTTTGGGCGTTCGTGTTCGGCTTCGCCGCAGGCGAAAGCCGAACACTCGCCGGTTTTAGACGCTGTTACAACTCTACTGCCGAT
GAGACGATTTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTCGAGCATGGTCTCGACGAGGTCGCCGTTC
CTAACGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGC
CCGCCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAATGCCACAGAGCAGACGATTGAACAGCTCGATACTGCTAACGAGAAAACGCACGAC
AGCACCTTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCATGCTGTTCGATCTCGCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAATG
ACGGCTACTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACCGGAGAGCTACGGGAATGGCGCGGCCGCGCCATTCCCTTAGAGGGCAAGCA
GCTCCGAGCTGTTCTCGATGATCTTGACCGGAAATACATCGATGTGGAGGTCGAAGTCGAATTCAAACGGGGGCCGTACAATGGGACACAGTCGCTGGAT
ACGAAGCGGTTTCGCGTCGTCGGCGTCCGCGACGAGGACGCCGACGACTACCACCTGTACATGACGAATTTAGCGAGGAAAGAGTTCTTTCCGGCGGATT
TAGCGGAGATCTACCGCTGTCGGTGGGAAGTTGAGTTGCTGTTCCGGGAGCTGAAGACGCAGTACGAATTGGACGAGTTCGACACGAGTGACGAACACGT
GGTGAGGATCTTATTGTACGCAGCGCTGCTGTCGCTGCTTGTAAGCCGCGATCTGTTGGATCTAGTCACTGAGCAGGCGGATGATGAGCTTGTGTTTCCG
ACAGAGCGCTGGGCGGCGACCTTTCGGTCGCACGCCCAGCTTATTCTCCACGAACTCGGTGAGTTCCTTGGCTACTCACCACCGCCGCTTCTCGACCGGC
TGATCGAAGACGCTCAAAAGATCCACAAGCAACGACCAATCTTACAAGAGACGCTCGCTACCACTACACAACCGAGGTGTGAGGCTTAACTAAAGACGGA
TG
CCTCACCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTCGAAGACCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCT
CCAGATCCCTGCCTTCGTTTGGGCGTTCGTGTTCGGCTTCGCCGCAGGCGAAAGCCGAACACTCGCCGGTTTTAGACGCTGTTACAACTCTACTGCCGAT
GAGACGATTTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTCGAGCATGGTCTCGACGAGGTCGCCGTTC
CTAACGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGC
CCGCCACGAGGAGCAGGCTGGAGCGAAGCTCCACCTGCTCCACAATGCCACAGAGCAGACGATTGAACAGCTCGATACTGCTAACGAGAAAACGCACGAC
AGCACCTTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCATGCTGTTCGATCTCGCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAATG
ACGGCTACTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACCGGAGAGCTACGGGAATGGCGCGGCCGCGCCATTCCCTTAGAGGGCAAGCA
GCTCCGAGCTGTTCTCGATGATCTTGACCGGAAATACATCGATGTGGAGGTCGAAGTCGAATTCAAACGGGGGCCGTACAATGGGACACAGTCGCTGGAT
ACGAAGCGGTTTCGCGTCGTCGGCGTCCGCGACGAGGACGCCGACGACTACCACCTGTACATGACGAATTTAGCGAGGAAAGAGTTCTTTCCGGCGGATT
TAGCGGAGATCTACCGCTGTCGGTGGGAAGTTGAGTTGCTGTTCCGGGAGCTGAAGACGCAGTACGAATTGGACGAGTTCGACACGAGTGACGAACACGT
GGTGAGGATCTTATTGTACGCAGCGCTGCTGTCGCTGCTTGTAAGCCGCGATCTGTTGGATCTAGTCACTGAGCAGGCGGATGATGAGCTTGTGTTTCCG
ACAGAGCGCTGGGCGGCGACCTTTCGGTCGCACGCCCAGCTTATTCTCCACGAACTCGGTGAGTTCCTTGGCTACTCACCACCGCCGCTTCTCGACCGGC
TGATCGAAGACGCTCAAAAGATCCACAAGCAACGACCAATCTTACAAGAGACGCTCGCTACCACTACACAACCGAGGTGTGAGGCTTAACTAAAGACGGA
TG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1275 bp | 424 aa | 115 | 1389 | + | No |
Chemistry : DDE
ORF sequence :
MRRLTTLFPSEFLEDHAEELGVVERDRKLQIPAFVWAFVFGFAAGESRTLAGFRRCYNSTADETISPGGFYQRLTPTLAEYLRDLVEHGLDEVAVPNAVD
ADIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKLHLLHNATEQTIEQLDTANEKTHDSTLFKTGPWLENRLMLFDLAYFKYRRFALIDENDGYFV
SRLKQNANPLITGELREWRGRAIPLEGKQLRAVLDDLDRKYIDVEVEVEFKRGPYNGTQSLDTKRFRVVGVRDEDADDYHLYMTNLARKEFFPADLAEIY
RCRWEVELLFRELKTQYELDEFDTSDEHVVRILLYAALLSLLVSRDLLDLVTEQADDELVFPTERWAATFRSHAQLILHELGEFLGYSPPPLLDRLIEDA
QKIHKQRPILQETLATTTQPRCEA
ADIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKLHLLHNATEQTIEQLDTANEKTHDSTLFKTGPWLENRLMLFDLAYFKYRRFALIDENDGYFV
SRLKQNANPLITGELREWRGRAIPLEGKQLRAVLDDLDRKYIDVEVEVEFKRGPYNGTQSLDTKRFRVVGVRDEDADDYHLYMTNLARKEFFPADLAEIY
RCRWEVELLFRELKTQYELDEFDTSDEHVVRILLYAALLSLLVSRDLLDLVTEQADDELVFPTERWAATFRSHAQLILHELGEFLGYSPPPLLDRLIEDA
QKIHKQRPILQETLATTTQPRCEA
Blast result :
Comments
One copie is present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 14194-15595.
97% aa homologous with ISH8D, 98% ADN homologous with ISH26 and ISH26-1, 93% ADN with ISH8.
97% aa homologous with ISH8D, 98% ADN homologous with ISH26 and ISH26-1, 93% ADN with ISH8.
References
1] Wailap, V. Ng., Stacy A. Ciufo, Smith, T.M., Bumgarner, R.E., Baskin, D., Faust, J., Hall, B., Loretz, C., Seto, J., Slagel, J., Hood, L., and DasSarma, S. (1998) Genome Research 8, 1131-1141