ISSphsp14

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Sphingobium sp.
Sphingobium sp.
Sphingobium sp. SA2
DNA section
IS Length : 2460 bp

Ends


IR Length : 17/22

IRL : GTAAGCGGTGTCCTGCCCCCACCTTGCGCAGTTGAAGCGCAGCGTTGAGT
IRR : GTAAGCGGTGTTTTCAGCCCACGGCACTCCATGGCATGAGGTCGCCGACG

Insertion site


Left flankDirect repeatRight flankDR Length
CGGCATGTGGAGTGATTCTGCATCGAAT8
TCCTGGAGTGGATCGAACAGCGTCGAGT8

DNA sequence

GTAAGCGGTGTCCTGCCCCCACCTTGCGCAGTTGAAGCGCAGCGTTGAGTTTCCTGGCCCTGAACGGGCTGGAGAGCGCTGCTACTATGATAGTCTCGGG
TGATGATCCTGTGAGCAAAGGCGCGCATCGGTTTGAGGTGTTCACGGGCGCCGGCAAGCGGCGGGATTGGCCGCCGGAAGTGAAGGCCTCGATTGTCGCG
GAGTGTTATACCGGCCGCGAAGGCGTCAGTGCCGTGGCGCGTCGGCATGGACTGGATCCCTCGCAAGTTTACGCGTGGCGGCGGGATTTGCGCAAGCAGC
TCGAGGCCGAGGGGGTAATCGTACCGCCGACGGAACCGGGAGCGCCGTCGTTTGTGCCCGCCGTGGTCGAACCGAGCGTGGCGGCGGATACTGTTGCCAA
GCGTCGCCCTCGTCGCCGACGCCGCGCGACCGAAGCCGCCGTAGAGTTGGAGATCGATGGTGTCGCTGTGAAGATCGGCCGCGGCGCTGACGCGGGCACG
ATTACCGCGGTGATCGAAGCGCTCAGGACGCCCCGGTGATCGGACCTGGCGCCGGGGCGCGCGTGATGGTGGCGACACGACCTGTGGATTTTCGCAAGGG
TGCGGATTCGCTCGCGGCGCTGGTCGGCGCCGAGTACGGCGGCGATCCCTATTCGGGGGTGATCTACGTGTTCCGGGCCAAGCGCGCAGACCGGATCAAG
CTGGTGTGGTGGGACGGCACCGGCCTGTGCCTGATGGCCAAGAAGCTGGAGAGCGGCGGGTTCAAGTGGCCGGGCATCCAGGACGGCGTGATGCGCGTGA
CGGCGGCGCAACTTGGCGCTTTGCTGGAAGGTCTGGACTGGCGCCGGGTGCATGGCGGACGTCGCCCCATTGCCCCGCAGATTGCTGGTTGACGGGGCAT
TCTTCTGCTGATTCACTACTGTCATGCTGATGGAAGCGGACCTTCCCGATGACGTCGAAGCGCTGCGTGCGCTTATCCGCGAACAGGCCCGCGAACTCGA
TGCGCTCAAGGTTTTCCAGGCTGAGGTCGAGCGCCTGAAGGCGATTATAGAGGCCCTCCAGCGTCACCGTTTCGGTCGTCGCTCGGAGCAGCTGGATCCC
GACCAGCTTCAGCTTGCCCTGGAGGAAGTCGAGACGGCCTTGGCCGAGGCGGAGCACGCGCACGACAAGGCAAGCCGGAGGCAAGCCGCTCGTCCGCGCA
AGACCAACCGCGGTTCACTGCCGGCTCATCTCGAACGGATCGAGCAAGTCGTCGACGTCGAGGACAAGGCCTGTCCGTGCTGCGGCGACGCGCTCCACCA
GATCGGCGAGGACGTGGCCGAGCGCCTCGACGTCGTGCCCACCACTTTCCGCGTCCTCGTCACCCGCCGGCCGCGCTACGGCTGTCGTTCGTGCGAGAGC
GCAGTCGTTCAGGCCCCGGCACCAGCACGGATCGTCGAGGGCGGTATTCCCACCGAGGCGCTGATCGCCCAGGTGCTCGTCGCCAAGTACGCCGATCACC
TGCCGCTCTACCGGCAGGCCCAGATCTACGCCCGGCAAGGCATCCAGCTTGATCGATCCACCCTGGCTGACTGGGTGGGTCGGGCAGCCTGGTATCTGCG
CCCCTTGCGTGATCACATCCTCGAACGGCTTCGACGATCCGAACGGCTGTTCGCGGACGAGACGACTGCGCCGGTGCTCGATCCGGGGCGTGGGCGGACC
AAGACCGGCCAGCTATGGGCCTATGCCCGCGACGACCGACCTTGGGGCGCCGATGATCCGCCGATGGTCGCCTATGTCTATGCGGCCGATCGCAGGGGCG
AACGGGCAGAAGCGCATCTCGGCGATTTTGCAGGTATCCTGCAGGTCGATGGCTATGGCGGCTATGCCGCGCTCGCCAGGCGTCGTCAGCAGATCAGCCT
TGCCTTTTGCTGGGCACACGTCCGGCGCAAGTTCTACGAGCTGGCCGACAGCTCTCCGGTGGCAACGGAAGTGCTGCGTCGCGTCGCCTTGCTCTATGCC
ATCGAAGATGAGGTGCGAGGATCATCGGCGGAGCAACGCCGGGCTGTACGCCACGACCGCAGCCGCATCATCGTCGATGACCTTCGCCAATATCTCGATG
CCCGCAATCGCCAGGTCAGCGCCAAGAGCAAGATCGGCGAAGCGATCCGCTATGCGCTCAACCGCTGGGATGGCCTGTCGCGCTTCCTGGACGATGGTCG
CATCGACCTCGACAGCAACACCGTCGAACGCTCTATCCGCCCCCTCGCGCTCAATCGGAAGAATGCGCTGTTCGCCGGCTCCGATGAAGGCGGCGACAAC
TGGGCGGTGATCGCCACGCTCATCGAGAACTGCAAACTCTCCGGCACCAACCCGAATATCTGGCTGACCGAAACCCTCACCAGCCTGGCCAATGGTCATC
CCGCAAACAGCGTCGGCGACCTCATGCCATGGAGTGCCGTGGGCTGAAAACACCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
453 bp150 aa87539+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MIVSGDDPVSKGAHRFEVFTGAGKRRDWPPEVKASIVAECYTGREGVSAVARRHGLDPSQVYAWRRDLRKQLEAEGVIVPPTEPGAPSFVPAVVEPSVAA
DTVAKRRPRRRRRATEAAVELEIDGVAVKIGRGADAGTITAVIEALRTPR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
357 bp118 aa536892+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

VIGPGAGARVMVATRPVDFRKGADSLAALVGAEYGGDPYSGVIYVFRAKRADRIKLVWWDGTGLCLMAKKLESGGFKWPGIQDGVMRVTAAQLGALLEGL
DWRRVHGGRRPIAPQIAG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1524 bp507 aa9242447+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEADLPDDVEALRALIREQARELDALKVFQAEVERLKAIIEALQRHRFGRRSEQLDPDQLQLALEEVETALAEAEHAHDKASRRQAARPRKTNRGSLPAH
LERIEQVVDVEDKACPCCGDALHQIGEDVAERLDVVPTTFRVLVTRRPRYGCRSCESAVVQAPAPARIVEGGIPTEALIAQVLVAKYADHLPLYRQAQIY
ARQGIQLDRSTLADWVGRAAWYLRPLRDHILERLRRSERLFADETTAPVLDPGRGRTKTGQLWAYARDDRPWGADDPPMVAYVYAADRRGERAEAHLGDF
AGILQVDGYGGYAALARRRQQISLAFCWAHVRRKFYELADSSPVATEVLRRVALLYAIEDEVRGSSAEQRRAVRHDRSRIIVDDLRQYLDARNRQVSAKS
KIGEAIRYALNRWDGLSRFLDDGRIDLDSNTVERSIRPLALNRKNALFAGSDEGGDNWAVIATLIENCKLSGTNPNIWLTETLTSLANGHPANSVGDLMP
WSAVG

 

Blast result :
Comments
ISSphsp14 is 76% aa (transposase) similar to ISFpe1.
References
1] Maurizio Labbate (2021) Direct submission.