ISSphsp11

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Sphingobium sp.
Sphingobium sp.
Sphingobium sp. SA2
DNA section
IS Length : 2826 bp

Ends


IR Length : 17/22

IRL : GTAACCGACCGGTTGAGGCCGCCTGCCTTGCTGTGATGTGAGCGCGTAAG
IRR : GTAACCGTCCGATTGTTACCGCGGCTTATGGGCCTTGAAGCGCGCGGCGA

Insertion site


Left flankDirect repeatRight flankDR Length
TGTCCTGGCCAGACGATCCTGCCTCGGC8
GGCCGACCTTGATGCCTGGATGGAAAGC8
GCTGGCATCTCAGGCGGCATCGCGAAAC8
TCCTGGAGTGGATCGAACAGCGTCGAGT8
TGTGCCGCCCGTAGCTCCGGGTCGCGCG8
CTGTTCGATCCATCAATCTT0

DNA sequence

GTAACCGACCGGTTGAGGCCGCCTGCCTTGCTGTGATGTGAGCGCGTAAGAGACGTCCATAAGGACGTCGTTATGGACGTCCTGAGCCAAAGCGAGGGAC
GGCGGTGGAGATATTGGGCCGGGAACGGCGTCGTCGGTGGAGCAACGCGGAGAAGCTCGAGATCGTCGCCGCTGTGGGAATGAACGGAGAGACGTTGGCG
CGCGTCGCGCGGCGCTACGATGTGTCGCGAAGCCAGATCTATCAATGGCGCCATCTGTTCAGGAAGCGAGGGTTGCTGCCTGCGGCGGACGGGCCGACCT
TTTTGCCGGTTGATATCGGGGCGCCCATGCTGAGCGCGGAGCCCGTGCTCGATGACCGGGTGGTGGTCTCGCCCTTGATCGTCGAGCTGTGTCTGGCGCA
AGGACGGCGGCTGCGCTTCGATGCCGGCATCGAGGCTACTGCCTTGACCCAGCTTATCCGGTCGGTGGAAGCTGCATGATCGGACCGGGCACCGGCGTAA
GGGTCTATCTCGCCTGCGGCGTCACTGACATGCGCAAAGGGATTTCGGGTCTTGCAATGCTGGCCCAGACCGTGCTGCGACAGAAGCCGGCCAGTGGTGC
CGTATTCGCCTTTCGGGGACGGCGCGGCGACAGGCTGAAGCTGCTTTACTGGGATGGTCAGGGATTTTGCCTCTATTACAAGATCCTGGAGCGCGGCCGT
TTTCCCTGGCCCTCTGCGGCTGACGGGGCGGCGCGACTGACATCGGCCCAACTCGCGATGCTGTGGGAAGGGATCGACTGGAGGCGTCCGAACTGGGGTG
CGCCGCCTACACGCGCGGGGTAACTTTTCTTCTGCAAAAGCGGCTGTTCTGCTAGGCTTTGCGGCCGATTTCCTGTATAAAAAGCCATGCTCAACGCGGC
ATCCGATCTTCCCGAAGACCCGGCCCTTCTCAAGGCGCTGATCGCCCAGTTGCAGGCCCAGAACAAGAAGCTGACGACGACGCTGCGCGCCCATGACCTG
GTCGTCCAGGCACTGCGTTTGCAGATCGCCAGGCTCAGGAAGCAGGCTTTCGGCAAATCCTCCGAGAAGATCGAACGCGAGATCGAGCAACTCGAGTTCG
CTCTCGAAGACGTGCTTGTTTCGGTCGCGCAACAGGGCCTCGTGACAAGTGGCCTGGACGATTCCGAGGCGCAAGCGGCGGTCGCGTCTGCCGAGACCAC
CCCTGGGCCGCGTCCATCTCGGCGCCCACGCGTCTCGGCGGATACGCCGCGCCAACGCCACGAACTTGACCCCGGCAGCACCTGTCCGGAGTGCGGCGGC
GAGCTGCGTCTCGTCGGCGAAGACGTGAGCGAGATCCTCGAGATGATCACGGCAAAGCTCCAGGTCATCGAAGTCGTCCGCCCGAAGAAGTCCTGCCGTT
GCTGCGAGAAGATGGTCCAGGTGCCAGCCCCGAGCCGACCGATCCCCGGTAGCATGGCCGGAGGAAGTCTCCTGGCTTACGTGCTGATCTCGAAGTTTGA
CGACCATTTGCCGCTGTACCGGCAGAACGAGATCATCGCGCGCATGGGTGCCGACATCCCACGCAGCACGCTGGCCGATTGGTGTGGTCGCTCGATGCGG
ATCCTGCAGCCGGTGATCGACCGGATCGAGGCCTCGGTCCTGGGCAGCGACATCCTGCACGCCGACGATACACCGATCCGCGTGCTGGCTCCGGAACGAC
GCGCCAAGGGTATCGGCAAGGGTGTGATGCAAGGCCGGATCTGGGGCTATGTCTGTGATCAGCGGCCTTGGGCCGGCACCGCGCCGCCTGGCGTCCTCTA
CCGCTATGCGCCCAACTGGAAAGCCGAGCACGTGCTGGCGCACTTGGGCAGCGCAAGCGGTATTCTTCAAGCCGACGCCTACAAAGGCTATGCTAAGCTC
TACGAACCCGTCGCGGATGGTGAGCCTCGCTTCCGGGAAGCGGCCTGCTTTGCGCATTGGCGACGCGATTTTCATGACATCTGGACGTCGCAAAAATCCG
AGATCGCACACGAGGCGCTCGAGCGTATCGGGCAACTCTACGACATTGAGCGCAAGATCGCGGGCAAGCCTGCCGATATCCGCCAGGCAATACGCCAGGA
GTTGAGCCGACCCAAGCTTGAGGCTCTGCATAGCTGGGCCGAGAAGCAACTCACCCGTATCTCGACCAAGGGAGACCTGGCGAAGGCCTTTCGCTACGCC
CTGGGGCGCTGGCACGCCTTCAGCCTCTTTATCGACGATGGACGCGTCGCCATCGACAACAACGCTGCCGAGCGGGCTGTCCGGCCCATATGCCTGGGCA
AGAAGAACTGGTTATTCGCCGGCTCCGAAACAGGCGCCGAAACCCTCGCCCGCGCCATGACGCTGATCGAAAGCGCCAAAATGAACGGTCTCGATCCACA
AGCATACATCACCGACTTGCTCAATCGCATCCACGATCACAAGATCAACAGGATCGACGAGCTGTTACCCTGGAACTGGGTGCCGCTCACCATCCCGCTA
GCACTGGCCGCCTGATGGCCGCCGTCACTTCCTTGCTCACCCTCGACTATGTCGCCAAGATACTCGACGAGAACGTGGAGTTGCTGGAAGCCATCGTTTC
AAACGACGACAACCTCACCTACGGCGCCATCGTCAGCGTCCACACCGGTCTGGACGAGTCCATCACCGCGCTGACGGATCACGGCGTGGAAGAACTGACG
GACATGCTTAGCCAAGCACGTCTCACGACCAAGGCCTGGCACCAATTCCTCGATGACTTTATCGACGATCCCGAGCTCGCCGCGCGCTTCAAGGCCCATA
AGCCGCGGTAACAATCGGACGGTTAC
Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
375 bp124 aa114488+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

VEILGRERRRRWSNAEKLEIVAAVGMNGETLARVARRYDVSRSQIYQWRHLFRKRGLLPAADGPTFLPVDIGAPMLSAEPVLDDRVVVSPLIVELCLAQG
RRLRFDAGIEATALTQLIRSVEAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa485832+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIGPGTGVRVYLACGVTDMRKGISGLAMLAQTVLRQKPASGAVFAFRGRRGDRLKLLYWDGQGFCLYYKILERGRFPWPSAADGAARLTSAQLAMLWEGI
DWRRPNWGAPPTRAG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1629 bp542 aa8962524+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLNAASDLPEDPALLKALIAQLQAQNKKLTTTLRAHDLVVQALRLQIARLRKQAFGKSSEKIEREIEQLEFALEDVLVSVAQQGLVTSGLDDSEAQAAVA
SAETTPGPRPSRRPRVSADTPRQRHELDPGSTCPECGGELRLVGEDVSEILEMITAKLQVIEVVRPKKSCRCCEKMVQVPAPSRPIPGSMAGGSLLAYVL
ISKFDDHLPLYRQNEIIARMGADIPRSTLADWCGRSMRILQPVIDRIEASVLGSDILHADDTPIRVLAPERRAKGIGKGVMQGRIWGYVCDQRPWAGTAP
PGVLYRYAPNWKAEHVLAHLGSASGILQADAYKGYAKLYEPVADGEPRFREAACFAHWRRDFHDIWTSQKSEIAHEALERIGQLYDIERKIAGKPADIRQ
AIRQELSRPKLEALHSWAEKQLTRISTKGDLAKAFRYALGRWHAFSLFIDDGRVAIDNNAAERAVRPICLGKKNWLFAGSETGAETLARAMTLIESAKMN
GLDPQAYITDLLNRIHDHKINRIDELLPWNWVPLTIPLALAA

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
297 bp98 aa25152811+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAAVTSLLTLDYVAKILDENVELLEAIVSNDDNLTYGAIVSVHTGLDESITALTDHGVEELTDMLSQARLTTKAWHQFLDDFIDDPELAARFKAHKPR

 

Blast result :
Comments
ISSphsp11 is 81% aa (transposase) similar to ISPpa5.
References
1] Maurizio Labbate (2021) Direct submission.