ISSphsp3
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Sphingobium sp. | Sphingobium sp. Sphingobium sp. SA2 Sphingobium sp. YG1 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 26/36
IRL : TGAAGTAGCCCCCAAAATCGCCTGACAGGCCCTCACACTTATGGAAGTGT
IRR : TGTTGTTGCCCCCTAAAGGCCGGACAGTTCCTCACGGGTTGAACGTTCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGTGCTGAC | GCCAT | TGGTGCTGAC | 5 |
| AAGTTCCTGATAGTC | CCGTC | GGTGTTGATTACTGC | 5 |
DNA sequence
TGAAGTAGCCCCCAAAATCGCCTGACAGGCCCTCACACTTATGGAAGTGTGATGCATATGCCTGACCGTATGACTGGAACATATGACCGCGTAGAGGTCA
TTACCTCGGTGCAGCGCCGTCGGCGCTGGACGCCCGAGGAGAAGATTCGCATCGTTGAGGAGACCTATTTGCCGGGGAACTCGGTGTCGCTGGTCGCCCG
CCGCCATGGCATCGCGGGCAACCAGCTATTCACCTGGCGACGGCTGATGACTCAGGGCGCACTCACAGCCGCTGCGGCCGGCGAGGAAGTTGTGGCCGCA
TCCGACTACCGCGCGCTTGAGGCGCAAGTCCGCGAACTGCAGCGCCTGCTCGGCAAGAAGACGATGGAGAACGAACTGCTGCGCGAGGCTGTCTCCCGGG
CCGCAGGCCCAAAAAAACAGCTGTTGCGCTCGACCTCGTGGCCCGAGGGTGGACGGTGAGCGCGGTTGCAAATGCCGTCGGCATGTCACGACCGCATCTC
TCCGCAATGCAACGACCAGCAGAACGACGGCGTCGCGGGCGTCCGCCCTTGCCCGACGCCGAACTCGCCGCCGATATCCGCAGGCTGATCGCCGACCTGC
CAACCTATGGCTATCGGCGCGTGCATGCCCTGCTGCGCCGCGAGGCGGAAAAAAACGGACGCGCAGCGCCCAATCCAAAGCGCGTCTACCGCGTCATGAA
GCTGCATGGCTTACTGCTCCAGCGCCACGCCGGACGCCGCGAGGAACGCCGGCATGACGGCAAGATCGCCGTCGTCACCCGCAATACACGCTGGTGTTCG
GACGGCCTCGAGATCGGCTGCGACAATGGCGAGAAGGTCCGCGTCGCCTTCGCCCTCGACTGCTGCGACCGGGAGGCGATGGGATATGTAGCCACTACCG
GCGGCATCACCGCCGAGGACGTGCGCGACCTCATGGTTGCCACCGTCGAGCATCGCTTCGGCCAGGTGAACAAGCTGCCCCACACCATCGAATGGCTGAC
TGATAACGGCAGCTGCTACACTGCCCAAGATACAAGGCGCTTCGCGCGCGACATCGGCCTGGCACCACGCACCACGCCGGTCGAAAGTCCGCAGAGCAAC
GGAATGGCCGAGGCTTTCGTCCGGACCCTCAAACGAGACTACGCCCGCGTCAGCCCGCGACCCAACGCACAGGCCGTCATCGACCAGCTGCCAAAATGGC
TCGACCACTATAATCGCGTGCATCCGCATCGCGCTCTCGGCTACAGATCGCCAAGAGAGTTTATCGAACGTTCAACCCGTGAGGAACTGTCCGGCCTTTA
GGGGGCAACAACA
TTACCTCGGTGCAGCGCCGTCGGCGCTGGACGCCCGAGGAGAAGATTCGCATCGTTGAGGAGACCTATTTGCCGGGGAACTCGGTGTCGCTGGTCGCCCG
CCGCCATGGCATCGCGGGCAACCAGCTATTCACCTGGCGACGGCTGATGACTCAGGGCGCACTCACAGCCGCTGCGGCCGGCGAGGAAGTTGTGGCCGCA
TCCGACTACCGCGCGCTTGAGGCGCAAGTCCGCGAACTGCAGCGCCTGCTCGGCAAGAAGACGATGGAGAACGAACTGCTGCGCGAGGCTGTCTCCCGGG
CCGCAGGCCCAAAAAAACAGCTGTTGCGCTCGACCTCGTGGCCCGAGGGTGGACGGTGAGCGCGGTTGCAAATGCCGTCGGCATGTCACGACCGCATCTC
TCCGCAATGCAACGACCAGCAGAACGACGGCGTCGCGGGCGTCCGCCCTTGCCCGACGCCGAACTCGCCGCCGATATCCGCAGGCTGATCGCCGACCTGC
CAACCTATGGCTATCGGCGCGTGCATGCCCTGCTGCGCCGCGAGGCGGAAAAAAACGGACGCGCAGCGCCCAATCCAAAGCGCGTCTACCGCGTCATGAA
GCTGCATGGCTTACTGCTCCAGCGCCACGCCGGACGCCGCGAGGAACGCCGGCATGACGGCAAGATCGCCGTCGTCACCCGCAATACACGCTGGTGTTCG
GACGGCCTCGAGATCGGCTGCGACAATGGCGAGAAGGTCCGCGTCGCCTTCGCCCTCGACTGCTGCGACCGGGAGGCGATGGGATATGTAGCCACTACCG
GCGGCATCACCGCCGAGGACGTGCGCGACCTCATGGTTGCCACCGTCGAGCATCGCTTCGGCCAGGTGAACAAGCTGCCCCACACCATCGAATGGCTGAC
TGATAACGGCAGCTGCTACACTGCCCAAGATACAAGGCGCTTCGCGCGCGACATCGGCCTGGCACCACGCACCACGCCGGTCGAAAGTCCGCAGAGCAAC
GGAATGGCCGAGGCTTTCGTCCGGACCCTCAAACGAGACTACGCCCGCGTCAGCCCGCGACCCAACGCACAGGCCGTCATCGACCAGCTGCCAAAATGGC
TCGACCACTATAATCGCGTGCATCCGCATCGCGCTCTCGGCTACAGATCGCCAAGAGAGTTTATCGAACGTTCAACCCGTGAGGAACTGTCCGGCCTTTA
GGGGGCAACAACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 390 bp | 129 aa | 70 | 459 | + | No |
Description : First part of the transposase
ORF sequence :
MTGTYDRVEVITSVQRRRRWTPEEKIRIVEETYLPGNSVSLVARRHGIAGNQLFTWRRLMTQGALTAAAAGEEVVAASDYRALEAQVRELQRLLGKKTME
NELLREAVSRAAGPKKQLLRSTSWPEGGR
NELLREAVSRAAGPKKQLLRSTSWPEGGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 325 aa | 321 | 1301 | + | No |
Description : Second part of the transposase
ORF sequence :
GASPRTAAPARQEDDGERTAARGCLPGRRPKKAVALDLVARGWTVSAVANAVGMSRPHLSAMQRPAERRRRGRPPLPDAELAADIRRLIADLPTYGYRRV
HALLRREAEKNGRAAPNPKRVYRVMKLHGLLLQRHAGRREERRHDGKIAVVTRNTRWCSDGLEIGCDNGEKVRVAFALDCCDREAMGYVATTGGITAEDV
RDLMVATVEHRFGQVNKLPHTIEWLTDNGSCYTAQDTRRFARDIGLAPRTTPVESPQSNGMAEAFVRTLKRDYARVSPRPNAQAVIDQLPKWLDHYNRVH
PHRALGYRSPREFIERSTREELSGL
HALLRREAEKNGRAAPNPKRVYRVMKLHGLLLQRHAGRREERRHDGKIAVVTRNTRWCSDGLEIGCDNGEKVRVAFALDCCDREAMGYVATTGGITAEDV
RDLMVATVEHRFGQVNKLPHTIEWLTDNGSCYTAQDTRRFARDIGLAPRTTPVESPQSNGMAEAFVRTLKRDYARVSPRPNAQAVIDQLPKWLDHYNRVH
PHRALGYRSPREFIERSTREELSGL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1232 bp | 409 aa | 70 | 1301 | + | Yes |
Chemistry : DDE
ORF sequence :
MTGTYDRVEVITSVQRRRRWTPEEKIRIVEETYLPGNSVSLVARRHGIAGNQLFTWRRLMTQGALTAAAAGEEVVAASDYRALEAQVRELQRLLGKKTME
NELLREAVSRAAGPKKAVALDLVARGWTVSAVANAVGMSRPHLSAMQRPAERRRRGRPPLPDAELAADIRRLIADLPTYGYRRVHALLRREAEKNGRAAP
NPKRVYRVMKLHGLLLQRHAGRREERRHDGKIAVVTRNTRWCSDGLEIGCDNGEKVRVAFALDCCDREAMGYVATTGGITAEDVRDLMVATVEHRFGQVN
KLPHTIEWLTDNGSCYTAQDTRRFARDIGLAPRTTPVESPQSNGMAEAFVRTLKRDYARVSPRPNAQAVIDQLPKWLDHYNRVHPHRALGYRSPREFIER
STREELSGL
NELLREAVSRAAGPKKAVALDLVARGWTVSAVANAVGMSRPHLSAMQRPAERRRRGRPPLPDAELAADIRRLIADLPTYGYRRVHALLRREAEKNGRAAP
NPKRVYRVMKLHGLLLQRHAGRREERRHDGKIAVVTRNTRWCSDGLEIGCDNGEKVRVAFALDCCDREAMGYVATTGGITAEDVRDLMVATVEHRFGQVN
KLPHTIEWLTDNGSCYTAQDTRRFARDIGLAPRTTPVESPQSNGMAEAFVRTLKRDYARVSPRPNAQAVIDQLPKWLDHYNRVHPHRALGYRSPREFIER
STREELSGL
Blast result :
Comments
ISSphsp3 is 77% aa similar to IS1312.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Maurizio Labbate (2021) Direct submission.