ISSphsp1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Sphingobium sp. | Sphingobium sp. Sphingobium sp. SA2 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 25/26
IRL : TGAAGCGCCCCGGGTTTCCAGGAGGCTCCAACTTGTGAGAAGGAGCATCC
IRR : TGAAGCGCCCCGGGTTTTCAGGAGGCAGTTTTCATTTGGCTATGCAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCGAAGCCG | aag | CCATTCTTCC | 3 |
| TGGCACGGCG | cag | CGCGAGATAG | 3 |
| ACACCGGGCA | ctc | CGTCCATCTG | 3 |
| CAACATCTAC | ATCAACGTGG | 0 | |
| ATCTGGGGCT | atg | TCTGTGATCA | 3 |
DNA sequence
TGAAGCGCCCCGGGTTTCCAGGAGGCTCCAACTTGTGAGAAGGAGCATCCGTTATGAGCAAGACAACCAACAAATTTTCACCTGAAGTCCGCGAGCGGGC
GGTTCGTATGGTGGTGGAGCAGCGGGCCGAGTACGGCTCGGAATGGGAAGTGATGAAGTCGATCGCGGCGAAGATCGGCTGCTCGCTGGAGACGCTGCGC
CGGTGGTGCCGCGAGGAGGCGAGCCGACGAGCGGGGCCAGCGGCGCTGGCGGCGGATGACCGGGAACGCCTGAGATTGCTGGAGCGCGAAGTGAAGGAAC
TACGCCGGGCCAACGAGATTTTGCGCAAGGCATCTGCGTATTTTGCGATGGCGGAGCTCGACCGCCCCGGGAGATGATGATGGCGTTCATCGACGTCCAT
CGCGAGGATTTGGGTATCGAGCCGATCTGCCGGGAACTGGCAATCGCCCCATCCTCCTACCATGAACACGCCGCGCGTAGTGCCGATCCCGGCAGGCGTC
CAGCACGTGCGCAGCGTGATGACGAGATCAGGGAGCAGATCAAGCGGGTCCATGAGGCCAGCTTCGGTCTCTATGGCGCACGCAAGGTCTGGCACCAGAT
GCGGCGTGAAGGCATCATGGTGGCGAAATGCACGGTGGAACGATTAATGCGCGCCATGGGGCTGGCAGGCGTCCGTCGTGGGAAGAAGACGATCACCACC
ATCAGCAACCCGAAGGCGCCGTGTCCGCTGGACAAGGTCAATCGGGCATTTCGCGTCAGCCGGCCAAACGCCCTGTGGGTCGTCGATTTCACCTACGTGC
ATACTTGGGCAGGCTTCGTCTATGTCGCCTTCGTGATTGATGCTTTTGCCCGGCGGATTGTCGGATGGAAGGTCAGCACGTCAGCCACTGCCAGCTTCGT
TCTGGATGCCCTGGAGCAGGCGATTCATGCCCGCAGGCCAGATCCCGATGATGGACTGATCCACCATAGCGATAGGGGAGTTCAATATCTTGCGATGAAC
TATACCCAGCGCTTGGCCGAGGCCAAACTCGTCCCCTCGGTCGGTAGCGTAGGTGATTCCTACGACAACGCCTTAGCCGAGACGATCAACGGCCTTTACA
AGGCCGAGGTCATCTGGCGGCAGCGATCATGGCCAAGCGTTTCGGCCGTGGAAATGGCAACGCTGCGCTGGGTCGATTGGTACAATAATCACCGGCTCTT
CGGCCCTATCGGGCACATCCCGCCCGCTGAGGCCGAAGACAATTACTATGCAGCCCTCGAGAACCTCGATATGGCTGCATAGCCAAATGAAAACTGCCTC
CTGAAAACCCGGGGCGCTTCA
GGTTCGTATGGTGGTGGAGCAGCGGGCCGAGTACGGCTCGGAATGGGAAGTGATGAAGTCGATCGCGGCGAAGATCGGCTGCTCGCTGGAGACGCTGCGC
CGGTGGTGCCGCGAGGAGGCGAGCCGACGAGCGGGGCCAGCGGCGCTGGCGGCGGATGACCGGGAACGCCTGAGATTGCTGGAGCGCGAAGTGAAGGAAC
TACGCCGGGCCAACGAGATTTTGCGCAAGGCATCTGCGTATTTTGCGATGGCGGAGCTCGACCGCCCCGGGAGATGATGATGGCGTTCATCGACGTCCAT
CGCGAGGATTTGGGTATCGAGCCGATCTGCCGGGAACTGGCAATCGCCCCATCCTCCTACCATGAACACGCCGCGCGTAGTGCCGATCCCGGCAGGCGTC
CAGCACGTGCGCAGCGTGATGACGAGATCAGGGAGCAGATCAAGCGGGTCCATGAGGCCAGCTTCGGTCTCTATGGCGCACGCAAGGTCTGGCACCAGAT
GCGGCGTGAAGGCATCATGGTGGCGAAATGCACGGTGGAACGATTAATGCGCGCCATGGGGCTGGCAGGCGTCCGTCGTGGGAAGAAGACGATCACCACC
ATCAGCAACCCGAAGGCGCCGTGTCCGCTGGACAAGGTCAATCGGGCATTTCGCGTCAGCCGGCCAAACGCCCTGTGGGTCGTCGATTTCACCTACGTGC
ATACTTGGGCAGGCTTCGTCTATGTCGCCTTCGTGATTGATGCTTTTGCCCGGCGGATTGTCGGATGGAAGGTCAGCACGTCAGCCACTGCCAGCTTCGT
TCTGGATGCCCTGGAGCAGGCGATTCATGCCCGCAGGCCAGATCCCGATGATGGACTGATCCACCATAGCGATAGGGGAGTTCAATATCTTGCGATGAAC
TATACCCAGCGCTTGGCCGAGGCCAAACTCGTCCCCTCGGTCGGTAGCGTAGGTGATTCCTACGACAACGCCTTAGCCGAGACGATCAACGGCCTTTACA
AGGCCGAGGTCATCTGGCGGCAGCGATCATGGCCAAGCGTTTCGGCCGTGGAAATGGCAACGCTGCGCTGGGTCGATTGGTACAATAATCACCGGCTCTT
CGGCCCTATCGGGCACATCCCGCCCGCTGAGGCCGAAGACAATTACTATGCAGCCCTCGAGAACCTCGATATGGCTGCATAGCCAAATGAAAACTGCCTC
CTGAAAACCCGGGGCGCTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 324 bp | 107 aa | 54 | 377 | + | No |
Description : First part of the transposase
ORF sequence :
MSKTTNKFSPEVRERAVRMVVEQRAEYGSEWEVMKSIAAKIGCSLETLRRWCREEASRRAGPAALAADDRERLRLLEREVKELRRANEILRKASAYFAMA
ELDRPGR
ELDRPGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 260 | 1282 | + | No |
Description : Second part of the transposase
ORF sequence :
PGTPEIAGARSEGTTPGQRDFAQGICVFCDGGARPPREMMMAFIDVHREDLGIEPICRELAIAPSSYHEHAARSADPGRRPARAQRDDEIREQIKRVHEA
SFGLYGARKVWHQMRREGIMVAKCTVERLMRAMGLAGVRRGKKTITTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAFARRI
VGWKVSTSATASFVLDALEQAIHARRPDPDDGLIHHSDRGVQYLAMNYTQRLAEAKLVPSVGSVGDSYDNALAETINGLYKAEVIWRQRSWPSVSAVEMA
TLRWVDWYNNHRLFGPIGHIPPAEAEDNYYAALENLDMAA
SFGLYGARKVWHQMRREGIMVAKCTVERLMRAMGLAGVRRGKKTITTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAFARRI
VGWKVSTSATASFVLDALEQAIHARRPDPDDGLIHHSDRGVQYLAMNYTQRLAEAKLVPSVGSVGDSYDNALAETINGLYKAEVIWRQRSWPSVSAVEMA
TLRWVDWYNNHRLFGPIGHIPPAEAEDNYYAALENLDMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1229 bp | 409 aa | 54 | 1282 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKTTNKFSPEVRERAVRMVVEQRAEYGSEWEVMKSIAAKIGCSLETLRRWCREEASRRAGPAALAADDRERLRLLEREVKELRRANEILRKASAYFCDG
GARPPREMMMAFIDVHREDLGIEPICRELAIAPSSYHEHAARSADPGRRPARAQRDDEIREQIKRVHEASFGLYGARKVWHQMRREGIMVAKCTVERLMR
AMGLAGVRRGKKTITTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAFARRIVGWKVSTSATASFVLDALEQAIHARRPDPDD
GLIHHSDRGVQYLAMNYTQRLAEAKLVPSVGSVGDSYDNALAETINGLYKAEVIWRQRSWPSVSAVEMATLRWVDWYNNHRLFGPIGHIPPAEAEDNYYA
ALENLDMAA
GARPPREMMMAFIDVHREDLGIEPICRELAIAPSSYHEHAARSADPGRRPARAQRDDEIREQIKRVHEASFGLYGARKVWHQMRREGIMVAKCTVERLMR
AMGLAGVRRGKKTITTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAFARRIVGWKVSTSATASFVLDALEQAIHARRPDPDD
GLIHHSDRGVQYLAMNYTQRLAEAKLVPSVGSVGDSYDNALAETINGLYKAEVIWRQRSWPSVSAVEMATLRWVDWYNNHRLFGPIGHIPPAEAEDNYYA
ALENLDMAA
Blast result :
Comments
ISSphsp1 is 93% aa similar to ISGdi14.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Maurizio Labbate (2021) Direct submission.