ISStma20

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP088244 ND Stenotrophomonas maltophilia
Stenotrophomonas maltophilia
Stenotrophomonas maltophilia 2013-SM4
DNA section
IS Length : 1431 bp

Ends


IR Length : 21/24

IRL : TGAAGTGATCCCCGGGAGTTGGACATCAGATCCAACCCCGTGGGATACAT
IRR : TGAAGTGACCCCCGAGACTTGGACGGTGGGGTCAGGCGGCTGGTTGGGCC

Insertion site


Left flankDirect repeatRight flankDR Length
ACACGCCGTAAACCCGTCCATGGGGGCTCGATGGCG3

DNA sequence

TGAAGTGATCCCCGGGAGTTGGACATCAGATCCAACCCCGTGGGATACATGAACAGATACGACGCGCGTTTCAAACTGCAGGTCGCCAAAGAGGCCTGCA
AGACCTCCACATCGGTCAAAGCCGTTGCTCGTCGCTACGGCCTGGAGTTCTCCACGGTCAGGCGCTGGGTGGCGACCTATCGGCTGCATGGTTGGCGCGG
GTTTCGCCGCCAGGCCCAGTCCTATGACCTCGCATTCAAGCTGGCTGTCCTGAAGAAGATGGGCCAGGACGGCATGTCCGGGCGGGAGGCCACCACTTAC
TTTCAAATTGGCGATGCCGGCGCGGTGGGGCGGTGGCGTCGGCTGTATGCTCAAGGCGGTGCCCAAGCGCTGGCACCACCTCCGCCGCCGCCCCGAAAGC
CGATGAAAAAGACCCGTTCGTCCAAGCCACCTGAGGAAATGAGCCGCGATGAGCTGCTCAAGGAAGTTGCCTATCTGCGTGCGGAGACCGACTACCTAAA
AAAACTCGATGCCTTGATCCGGGAAGAGCAGGCGGCGCAGGACGCAAAGCGCAAGCCATCCAAGGATTGAGGCAGGTTCATACGCTGTCGCTTCTGCTCG
AAGCGGCAGAGCTGTCACGCAGTACGTTCTATTACCAGAACCATGTCCTGGCCCATCCGGATCAGGATGAGGCAGCCCTGTGCGAGCGCATCCGGGCAAT
CTACGATCAAAGCCAAGGGCGCTATGGGTATCGCACTGTGACGCTGGAACTGGCCAATCAGGGCCATCGGACCAATCACAAGCGGGTACAGCGCCTGATG
GGGGAGATGGGCCTGAAATCACGGGTACGCGTGAAGCGCTACCGGTCCTTCAAAGGGGCCGCCAATGTTGTGGTTGGCAATGACCTCAATCGCCAGTTCC
ATGCCGAGCGGCCCAACCAGAAGTGGGTGACCGACGTCACCGAGTTCAAGGTGCAAGGCATGAAGCTGTACCTGTCGCCGATCATGGACCTCTACAACGG
TGAAATCGTGGCTTATCAGATCAAGCGCCAGCCTGTGTTCGATCTGGTAGGTCAGATGCTGGAGGAGGCCATCAGCAAGCTTTCGCCGGATGAGCGGCCA
ATGATCCACTCCGACCAGGGCTGGCAGTACCAGCATGAGAACTACCGGCACATGCTGGAAAAGCACTCGTTGAAGCAAAGCATGTCCCGACGTGGCAACT
GCTTGGACAATGCGGCAATGGAAAGCTTCTTCGGGACGCTGAAGTCGGAGTTCTTCTACCTGAACAGCTTTGACAGCATCGAGAGTCTGGAGGCCGGGCT
AGTGGAGTACATCAAGTACTACAACCAAGAGCGAATCAGATTGAAATTGAAGGGGCTAAGCCCGGTAAAGTACCGGGAACAGGCCCAACCAGCCGCCTGA
CCCCACCGTCCAAGTCTCGGGGGTCACTTCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
522 bp173 aa49570+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MNRYDARFKLQVAKEACKTSTSVKAVARRYGLEFSTVRRWVATYRLHGWRGFRRQAQSYDLAFKLAVLKKMGQDGMSGREATTYFQIGDAGAVGRWRRLY
AQGGAQALAPPPPPPRKPMKKTRSSKPPEEMSRDELLKEVAYLRAETDYLKKLDALIREEQAAQDAKRKPSKD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
948 bp315 aa4531400+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AAQGSCLSACGDRLPKKTRCLDPGRAGGAGRKAQAIQGLRQVHTLSLLLEAAELSRSTFYYQNHVLAHPDQDEAALCERIRAIYDQSQGRYGYRTVTLEL
ANQGHRTNHKRVQRLMGEMGLKSRVRVKRYRSFKGAANVVVGNDLNRQFHAERPNQKWVTDVTEFKVQGMKLYLSPIMDLYNGEIVAYQIKRQPVFDLVG
QMLEEAISKLSPDERPMIHSDQGWQYQHENYRHMLEKHSLKQSMSRRGNCLDNAAMESFFGTLKSEFFYLNSFDSIESLEAGLVEYIKYYNQERIRLKLK
GLSPVKYREQAQPAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1352 bp450 aa491400+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNRYDARFKLQVAKEACKTSTSVKAVARRYGLEFSTVRRWVATYRLHGWRGFRRQAQSYDLAFKLAVLKKMGQDGMSGREATTYFQIGDAGAVGRWRRLY
AQGGAQALAPPPPPPRKPMKKTRSSKPPEEMSRDELLKEVAYLRAETDYLKKTRCLDPGRAGGAGRKAQAIQGLRQVHTLSLLLEAAELSRSTFYYQNHV
LAHPDQDEAALCERIRAIYDQSQGRYGYRTVTLELANQGHRTNHKRVQRLMGEMGLKSRVRVKRYRSFKGAANVVVGNDLNRQFHAERPNQKWVTDVTEF
KVQGMKLYLSPIMDLYNGEIVAYQIKRQPVFDLVGQMLEEAISKLSPDERPMIHSDQGWQYQHENYRHMLEKHSLKQSMSRRGNCLDNAAMESFFGTLKS
EFFYLNSFDSIESLEAGLVEYIKYYNQERIRLKLKGLSPVKYREQAQPAA

 

Blast result :
Comments
ISStma20 is 98% aa similar to ISStma2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Xin Gao (2020) Direct submission.