ISVch12
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| Y | Vibrio cholerae | Vibrio cholerae Vibrio cholerae ICDC-VC1445 Vibrio cholerae ICDC1445 plasmid pICDC-VC1445 |
DNA section
IS Length : 1233 bp
Ends
IR Length : 21/30
IRL : TGATCTGATTTAATGGCACCGCACACTTTTGTGAAGGATAATTAGTCCAA
IRR : TGATCTGGTCAAGCTAGGCCGCACACTTTTTCCTTACACTTTCAAACTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAGCGTTAC | TAC | TACCACCGCT | 3 |
| TGAGGAGGCT | gct | GCTATGACAC | 3 |
DNA sequence
TGATCTGATTTAATGGCACCGCACACTTTTGTGAAGGATAATTAGTCCAACAACAAAGGTGTGAAAATGACCCAACGAAGAAAACCTAGAAAATATACTG
ATGAGTTCAGAGAAGAAGCTGTAAAGCTAGTCACTGAACAAGGTTACAGTGTTACTGAAGCAGCCAAATCATTAGGCATAACAACTAAGCTGCTTTACAA
CTGGAAAGACAAACTAGCCAAGCAAACTTCAGGCGAAGCATTAAGCAAAGATGAGAGAGCTGAACTGGTTAAGCTCAGAAAAGAGAATAAACGCTTACAG
ATGGAGCGTGAGATCTTAAAAAAGGCGAGTGCCTTCTTTGCGAAAGAAATGAAATAAAGTTCGAATATATTAAAGAGCAGCAATGGCGCTTTCCAATTAG
CGTTTTGTGCGAGGTTCTAGAAGTGAGTCGGTCAGCCTATTACGCATGGCTGAGGCGACCAGCCAAGATTATTAGTGTTGAAGAGTTAATGCTTTATCGT
CGCATGAAGAGGTTATTTGATGATAGCCGCAGTAGTGCTGGTGCCAGAACCTTAATGAAGTTGCTACGCAAAGAAGGCTTTAAAATTGGTATTTGTCGGG
TTAAGAGCTTGATGAAAAAGCTCGGTTTAGTCGTTAAACAGCGAGTGGCTTATAAAGTGACGACTATGCGCAAACATAGCCATGCGGTTGCTGATAACTT
ATTGAAGCGCCAATTCAATCCAGCCAGAGCAAATCAGGCGTGGGCGGGTGATATCACCTATCTAAGAACGCATCAAGGCTGGATGTACCTAGCTGTTGTG
ATGGATTTGCATTCTCGCCGTATTATTGGCTGGGCGTTGAGTAAACGCATGACGGTTGATTTAACCATGAGAGCGATGCAAATGGCTATCAACCTGCGTC
AACCCAAAGCAGGCTTGATCTTCCATAGCGACAGAGGCTCACAATACACCAGTAAGCGTTTTCAATCATTGCTATGGAGCAATCGAATTACGCCATCAAT
GAGTGGTTGTGGAGCATGTTTAGATAATGCTGTGGTTGAAAGGTTTTTCGGCAGCTTGAAAAATGAATGGCTATTAAATGTTTACCATTTAACCAGAGAA
AGCATGAAAACTGATGTAGAGAAATACATCAAATACTACAACTCAGTTCGGCTTCATACTTCATTAAATGATATGTCGCCAATTGAGTTTGAAAGTGTAA
GGAAAAAGTGTGCGGCCTAGCTTGACCAGATCA
ATGAGTTCAGAGAAGAAGCTGTAAAGCTAGTCACTGAACAAGGTTACAGTGTTACTGAAGCAGCCAAATCATTAGGCATAACAACTAAGCTGCTTTACAA
CTGGAAAGACAAACTAGCCAAGCAAACTTCAGGCGAAGCATTAAGCAAAGATGAGAGAGCTGAACTGGTTAAGCTCAGAAAAGAGAATAAACGCTTACAG
ATGGAGCGTGAGATCTTAAAAAAGGCGAGTGCCTTCTTTGCGAAAGAAATGAAATAAAGTTCGAATATATTAAAGAGCAGCAATGGCGCTTTCCAATTAG
CGTTTTGTGCGAGGTTCTAGAAGTGAGTCGGTCAGCCTATTACGCATGGCTGAGGCGACCAGCCAAGATTATTAGTGTTGAAGAGTTAATGCTTTATCGT
CGCATGAAGAGGTTATTTGATGATAGCCGCAGTAGTGCTGGTGCCAGAACCTTAATGAAGTTGCTACGCAAAGAAGGCTTTAAAATTGGTATTTGTCGGG
TTAAGAGCTTGATGAAAAAGCTCGGTTTAGTCGTTAAACAGCGAGTGGCTTATAAAGTGACGACTATGCGCAAACATAGCCATGCGGTTGCTGATAACTT
ATTGAAGCGCCAATTCAATCCAGCCAGAGCAAATCAGGCGTGGGCGGGTGATATCACCTATCTAAGAACGCATCAAGGCTGGATGTACCTAGCTGTTGTG
ATGGATTTGCATTCTCGCCGTATTATTGGCTGGGCGTTGAGTAAACGCATGACGGTTGATTTAACCATGAGAGCGATGCAAATGGCTATCAACCTGCGTC
AACCCAAAGCAGGCTTGATCTTCCATAGCGACAGAGGCTCACAATACACCAGTAAGCGTTTTCAATCATTGCTATGGAGCAATCGAATTACGCCATCAAT
GAGTGGTTGTGGAGCATGTTTAGATAATGCTGTGGTTGAAAGGTTTTTCGGCAGCTTGAAAAATGAATGGCTATTAAATGTTTACCATTTAACCAGAGAA
AGCATGAAAACTGATGTAGAGAAATACATCAAATACTACAACTCAGTTCGGCTTCATACTTCATTAAATGATATGTCGCCAATTGAGTTTGAAAGTGTAA
GGAAAAAGTGTGCGGCCTAGCTTGACCAGATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 67 | 357 | + | No |
Description : First part of the transposase
ORF sequence :
MTQRRKPRKYTDEFREEAVKLVTEQGYSVTEAAKSLGITTKLLYNWKDKLAKQTSGEALSKDERAELVKLRKENKRLQMEREILKKASAFFAKEMK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 301 aa | 312 | 1220 | + | No |
Description : Second part of the transposase
ORF sequence :
DLKKGECLLCERNEIKFEYIKEQQWRFPISVLCEVLEVSRSAYYAWLRRPAKIISVEELMLYRRMKRLFDDSRSSGARTLMKLLRKEGFKIGICRVKSLM
KKLGLVVKQRVAYKVTTMRKHSHAVADNLLKRQFNPARANQAWAGDITYLRTHQGWMYLAVVMDLHSRRIIGWALSKRMTVDLTMRAMQMAINLRQPKAG
LIFHSDRGSQYTSKRFQSLLWSNRITPSMSGCGACLDNAVVERFFGSLKNEWLLNVYHLTRESMKTDVEKYIKYYNSVRLHTSLNDMSPIEFESVRKKCA
A
KKLGLVVKQRVAYKVTTMRKHSHAVADNLLKRQFNPARANQAWAGDITYLRTHQGWMYLAVVMDLHSRRIIGWALSKRMTVDLTMRAMQMAINLRQPKAG
LIFHSDRGSQYTSKRFQSLLWSNRITPSMSGCGACLDNAVVERFFGSLKNEWLLNVYHLTRESMKTDVEKYIKYYNSVRLHTSLNDMSPIEFESVRKKCA
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1154 bp | 383 aa | 67 | 1220 | + | Yes |
Chemistry : DDE
ORF sequence :
MTQRRKPRKYTDEFREEAVKLVTEQGYSVTEAAKSLGITTKLLYNWKDKLAKQTSGEALSKDERAELVKLRKENKRLQMEREILKKGECLLCERNEIKFE
YIKEQQWRFPISVLCEVLEVSRSAYYAWLRRPAKIISVEELMLYRRMKRLFDDSRSSGARTLMKLLRKEGFKIGICRVKSLMKKLGLVVKQRVAYKVTTM
RKHSHAVADNLLKRQFNPARANQAWAGDITYLRTHQGWMYLAVVMDLHSRRIIGWALSKRMTVDLTMRAMQMAINLRQPKAGLIFHSDRGSQYTSKRFQS
LLWSNRITPSMSGCGACLDNAVVERFFGSLKNEWLLNVYHLTRESMKTDVEKYIKYYNSVRLHTSLNDMSPIEFESVRKKCAA
YIKEQQWRFPISVLCEVLEVSRSAYYAWLRRPAKIISVEELMLYRRMKRLFDDSRSSGARTLMKLLRKEGFKIGICRVKSLMKKLGLVVKQRVAYKVTTM
RKHSHAVADNLLKRQFNPARANQAWAGDITYLRTHQGWMYLAVVMDLHSRRIIGWALSKRMTVDLTMRAMQMAINLRQPKAGLIFHSDRGSQYTSKRFQS
LLWSNRITPSMSGCGACLDNAVVERFFGSLKNEWLLNVYHLTRESMKTDVEKYIKYYNSVRLHTSLNDMSPIEFESVRKKCAA
Blast result :
Comments
ISVch12 is 74% aa similar to ISSde6.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Two intact copies of ISVch12 were found in Vibrio cholerae ICDC1445 plasmid pICDC-VC1445.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Two intact copies of ISVch12 were found in Vibrio cholerae ICDC1445 plasmid pICDC-VC1445.
References
1] Bo Pang (2021) Direct submission.