ISPa140

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MN894887 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa SE5416 plasmid pSE5416-KPC
DNA section
IS Length : 2384 bp

Ends


IR Length : 21/24

IRL : GTAAGCGGCCAGCAAACACACCTTCAGAATCCCAGCATTAAGGGCGATGG
IRR : GTAAGCGAACCGCAAACACACCTTGCGCTCGTTAAGCTGGCGTCCACTGA

Insertion site


Left flankDirect repeatRight flankDR Length
GCAAGCAAGAACGTCTGGAGCACGCCAACCAG8

DNA sequence

GTAAGCGGCCAGCAAACACACCTTCAGAATCCCAGCATTAAGGGCGATGGTGTCCACCTAAGAATACGTGGACACCATCGCCCTTATCGCAAGGATCACC
ATGCGCCAGCGCACCTCTTATCCAAAACCGTTCAAAGCCCAAGTCGTCCAGGAATGTCTTCAACCTGACGTATCGATAGCCAGCATCGCGCTGCGCCATG
GCATCAACGCCAACCTGGTGCGCAAATGGATTCCGCTCTATCGTGACCGTCAACCTGACGCGCTACCGGCATTCGTGCCGCTGACGATGAAGCCCGAATC
TGAGCCTGCACAACCCACACTGGCGCGCATCGAAATTCCGTTCAAACAACAAACAGTCACAGTGATCTGGCCCGCTGTCGATCCCGATGGCTGTGCGCGC
TTCATTCGCGGTCTCGTCAAATGATTCGCATCGATACAATCTGGCTCGCCACCGAGCCCATGGACATGCGCGCCGGCACCGAAACGGCGTTGGCCCGCGT
CGTCGCGGTCTTCGGTGCGGCGAAGCCGCACTGCGCTTATCTCTTTGCCAACCGCCGCGCCAACCGGATGAAGGTGCTGGTGCATGACGGCATTGGCGTG
TGGTTGGCAGCACGCCGACTGAACCAAGGTAAATTCCACTGGCCGGGCGTTCGTCACGGCGCTCAAGTGGAACTGGACAGCGAGCAAGTTCAGGCACTCA
TCGTCGGATTACCTTGGCAACGCATGGGTGCCAACAGCACCATCACACGGCTTTAGTACTGACTATTGGCATAACCGCCAATCGCCGCCAGTGTGTTGCT
CTGGCATGATTGGCGGCATGACTTCTTTACCCGACCTCGACCAACTGACCCCTGCAGAGCTGCGCGCGTTGCTCGAGCAGACGCTGACATTGCAGTCTCA
AGTCGAGGCGATGAGTCGCAAGATCCAGAACGACAAGATCGTCATCGAGCAGCTCACTCATGAGATCGCTGTCCTCAAACGTCATAAGTACGCCAAACGC
AGCGAACAGATCAGTCCAGAGCAAGGCCGCTTGCTCGATGATTTGCTCAGCACTGACCTTGAGGCCATCGAAGCCGAGCTGAACGCACTTCATCCCGACC
CGGCCCCAAAAGAGCCAGGCCAGAAGCCCAAGCGCGCACCACTGCCTGCACAGCTTCCGCGCACCGTGATTCATCATGAACCCGACAACACCCAATGTGC
GTGTGGCTGCGAGCTTCAGCGCATCGGTGAAGATGTCAGCGAAAAGCTCGATTACACGCCGGGCGTGTTCACGGTTGAGCAGCATGTACGTGGAAAGTGG
GCCTGCCGAAGTTGCGAAACCCTGATCCAGGCACCGGTTCCGGCGCAAGTCATCGACAAAGGCATCCCCACCGCAGGTCTGCTGGCCCATGTCATGGTGG
CCAAGTTCGCTGACCACCTGCCGTTGTACCGGCAGGAAAAGATCTTTGGCCGCGCCGGCCTGGCGATTCCACGCTCGACACTGGCCCGGTGGGTCGGGCA
AACCGGCGCGCAGCTTCAACCGCTTGTCGATGCCCTGCGTGAAGCGGTGCTGGCTCAACCAGTCATCCACGCCGATGAAACACCGGTTCAGATGTTGGCA
CCGGGTGAAAAGAAAACCCATCGAAGTTATGTGTGGGCCTATAGCAGCACTGCTTACGCAGAGCTTAAATCCGTGGTTTACGACTTCAGCCCGAGCCGTG
CCGGCGAGCATGCGCGTAACTTCCTCGGCGACTGGCGCGGCAAGTTGGTGTGCGATGACTTTGCCGGCTACAAGGCCAGTTTCGAGCAGGGCATCGTCGA
GATTGGCTGCATGGCCCATGCGCGCCGCAAGTTCTTTGACTTGCATGCGGCGAACAAGAGCCAGTTGGCCGAGCAGGCCCTTCTGACAATCGGCGCGCTC
TACGAGGTCGAACGGCAGGCCAGGGACATGAGCGAAGAAGACCGCCGGCGAATACGTCAGCGGGATGCGATTCCCATCGCTGAAAAACTGCATGACTGGA
TGCTGACCCAGCGCGAACTGGTACCCGAAGGCTCAGCCATCGCCAAAGCTCTGGATTACAGCTTGAAACGCTGGGTAGCGCTGACGCGCTACCTGGACGA
CGGCGCCTTGCCCATCGATAACAACCACGTCGAGAACCAAATACGACCGTGGGCACTTGGGCGTGCCAATTGGTTGTTTGCAGGATCACTGCGCAGTGGC
AAGCGGGCGGCGGCGATCATGAGCCTGATTCAGTCGGCGCGCATGAATGGACATGATCCGTATGCCTATCTCAAGGACGTGTTGACGCGACTGCCAACAC
AGCGGGCTAGCGAGATTGAAGCTTTGCTGCCGCATCAGTGGACGCCAGCTTAACGAGCGCAAGGTGTGTTTGCGGTTCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
324 bp107 aa101424+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MRQRTSYPKPFKAQVVQECLQPDVSIASIALRHGINANLVRKWIPLYRDRQPDALPAFVPLTMKPESEPAQPTLARIEIPFKQQTVTVIWPAVDPDGCAR
FIRGLVK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
336 bp111 aa421756+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRIDTIWLATEPMDMRAGTETALARVVAVFGAAKPHCAYLFANRRANRMKVLVHDGIGVWLAARRLNQGKFHWPGVRHGAQVELDSEQVQALIVGLPWQ
RMGANSTITRL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1548 bp515 aa8062353+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIGGMTSLPDLDQLTPAELRALLEQTLTLQSQVEAMSRKIQNDKIVIEQLTHEIAVLKRHKYAKRSEQISPEQGRLLDDLLSTDLEAIEAELNALHPDPA
PKEPGQKPKRAPLPAQLPRTVIHHEPDNTQCACGCELQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRSCETLIQAPVPAQVIDKGIPTAGLLAHVMVAK
FADHLPLYRQEKIFGRAGLAIPRSTLARWVGQTGAQLQPLVDALREAVLAQPVIHADETPVQMLAPGEKKTHRSYVWAYSSTAYAELKSVVYDFSPSRAG
EHARNFLGDWRGKLVCDDFAGYKASFEQGIVEIGCMAHARRKFFDLHAANKSQLAEQALLTIGALYEVERQARDMSEEDRRRIRQRDAIPIAEKLHDWML
TQRELVPEGSAIAKALDYSLKRWVALTRYLDDGALPIDNNHVENQIRPWALGRANWLFAGSLRSGKRAAAIMSLIQSARMNGHDPYAYLKDVLTRLPTQR
ASEIEALLPHQWTPA

 

Blast result :
Comments
ISPa140 is 89% aa similar (transposase) to ISPa122.
References
1] Hong Du (2021) Direct submission.