ISPa139
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MN894887.1 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas aeruginosa SE5416 plasmid pSE5416-KPC Pseudomonas furukawaii |
DNA section
IS Length : 1169 bp
Ends
IR Length : 21/26
IRL : GGAAACTCCGGAAAAGTCCCGACCGATCTGCTGAAATGACGCCCCCGCAG
IRR : GGAAACTCCGAACAAGTCAGCGACGGGCAGCCGATTGGCGACCGAATCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCGAGGCATAGGCTC | TTAG | GTGTCCTTGTCTC | 4 |
DNA sequence
GGAAACTCCGGAAAAGTCCCGACCGATCTGCTGAAATGACGCCCCCGCAGAACCTGAGCCCTGCCGCATGACCCAGATGAGCTTTTCCGATGCCGAGTAC
GCCGGCAAACGCAAACAGACCCGGCGCGAGCGCTTTCTGGCGGAGATGGAGCAAGTGGTGCCCTGGGCCGGGCTGTTGGCGCTGATCGAGCCGTACTACC
CCAAGGCGGGCAATGGCCGTCCGCCGTATCCGCTGGAAACCATGCTGCGCATCCACCTGCTGCAGAACTGGTTCGCTCTCAGCGATCCGGCGATGGAGGA
AGCGCTGTACGAGATCACCTCGCTGCGTCAGTTCGCCCAGCTGTCGCTGACCCAGGGCAGCATCCCGGAAGACACGACAATCATGAATTTCCGCCACCTG
CTGGAGCAGCACGGATTGGCCGCGGGCATCCTGGCGGTGATCAACGACCACCTGGGCGAGCGCGGGTTGTCACTGCGCCAGGGCACCATCGTTGATGCCA
CGATCATTCATGCGCCGAGTTCGACCAAGAACCGGGAAGGCAAGCGTGATCCGGAGATGCACCAGACGAAGAAGGGCAACCAGTACTTCTTCGGCATGAA
GGCACATATCGGCGCCGATGCCGAGTCCGGCCTGGTGCACAGCGTCGTGGGGACGGCGGCCAACGTGGCCGATGTCACCCAGGTCGACAAACTGCTGCAC
GGCGAGGAGAACGTGGTGTGTGCCGATGCGGGCTACACCGGGGTGGAGAAACGTCCCGAGCATGCCGGGCGTGAGGTCATCTGGCAGATCGCCGCCCGGC
GCAGCACCTACAAACAACTGAGCAAACGCAGTGCCCTGTACAAGGCGAGGCGCAAGATCGAGAAGGCAAAAGCGCAGGTGCGGGCCAAGGTCGAGCATCC
ATTCCGGGTGATCAAACGGCAGTTCGGCTACACCAAAGTGCGCTTCCGGGGCCTGGCCAAGAACACTGCGCAACTGGTCACCTTGTTCGCCCTGTCGAAT
CTATGGATGGCCCGCCGAGAGTTGCTGGCTGAAACGGGAGAGGTGCGCCTGTAAAACGCAGAAACCCGGTCAAAAGCCGGGTTTCTGGTGAAGTCTGTCG
CCGAAGACCGGGCTAAAAATCGATTCGGTCGCCAATCGGCTGCCCGTCGCTGACTTGTTCGGAGTTTCC
GCCGGCAAACGCAAACAGACCCGGCGCGAGCGCTTTCTGGCGGAGATGGAGCAAGTGGTGCCCTGGGCCGGGCTGTTGGCGCTGATCGAGCCGTACTACC
CCAAGGCGGGCAATGGCCGTCCGCCGTATCCGCTGGAAACCATGCTGCGCATCCACCTGCTGCAGAACTGGTTCGCTCTCAGCGATCCGGCGATGGAGGA
AGCGCTGTACGAGATCACCTCGCTGCGTCAGTTCGCCCAGCTGTCGCTGACCCAGGGCAGCATCCCGGAAGACACGACAATCATGAATTTCCGCCACCTG
CTGGAGCAGCACGGATTGGCCGCGGGCATCCTGGCGGTGATCAACGACCACCTGGGCGAGCGCGGGTTGTCACTGCGCCAGGGCACCATCGTTGATGCCA
CGATCATTCATGCGCCGAGTTCGACCAAGAACCGGGAAGGCAAGCGTGATCCGGAGATGCACCAGACGAAGAAGGGCAACCAGTACTTCTTCGGCATGAA
GGCACATATCGGCGCCGATGCCGAGTCCGGCCTGGTGCACAGCGTCGTGGGGACGGCGGCCAACGTGGCCGATGTCACCCAGGTCGACAAACTGCTGCAC
GGCGAGGAGAACGTGGTGTGTGCCGATGCGGGCTACACCGGGGTGGAGAAACGTCCCGAGCATGCCGGGCGTGAGGTCATCTGGCAGATCGCCGCCCGGC
GCAGCACCTACAAACAACTGAGCAAACGCAGTGCCCTGTACAAGGCGAGGCGCAAGATCGAGAAGGCAAAAGCGCAGGTGCGGGCCAAGGTCGAGCATCC
ATTCCGGGTGATCAAACGGCAGTTCGGCTACACCAAAGTGCGCTTCCGGGGCCTGGCCAAGAACACTGCGCAACTGGTCACCTTGTTCGCCCTGTCGAAT
CTATGGATGGCCCGCCGAGAGTTGCTGGCTGAAACGGGAGAGGTGCGCCTGTAAAACGCAGAAACCCGGTCAAAAGCCGGGTTTCTGGTGAAGTCTGTCG
CCGAAGACCGGGCTAAAAATCGATTCGGTCGCCAATCGGCTGCCCGTCGCTGACTTGTTCGGAGTTTCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 987 bp | 328 aa | 68 | 1054 | + | No |
Chemistry : DDE
ORF sequence :
MTQMSFSDAEYAGKRKQTRRERFLAEMEQVVPWAGLLALIEPYYPKAGNGRPPYPLETMLRIHLLQNWFALSDPAMEEALYEITSLRQFAQLSLTQGSIP
EDTTIMNFRHLLEQHGLAAGILAVINDHLGERGLSLRQGTIVDATIIHAPSSTKNREGKRDPEMHQTKKGNQYFFGMKAHIGADAESGLVHSVVGTAANV
ADVTQVDKLLHGEENVVCADAGYTGVEKRPEHAGREVIWQIAARRSTYKQLSKRSALYKARRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNT
AQLVTLFALSNLWMARRELLAETGEVRL
EDTTIMNFRHLLEQHGLAAGILAVINDHLGERGLSLRQGTIVDATIIHAPSSTKNREGKRDPEMHQTKKGNQYFFGMKAHIGADAESGLVHSVVGTAANV
ADVTQVDKLLHGEENVVCADAGYTGVEKRPEHAGREVIWQIAARRSTYKQLSKRSALYKARRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNT
AQLVTLFALSNLWMARRELLAETGEVRL
Blast result :
Comments
ISPa139 is 93% aa similar to IS2000.
References
1] Hong Du (2021) Direct submission.