ISPmo5

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MN310371 ND Pseudomonas monteilii
Pseudomonas monteilii QJ20133 plasmid pJ20133-VIM
DNA section
IS Length : 1235 bp

Ends


IR Length : 25bp

IRL : TGTAGTGGTCTACTAACCCCGGACACCTTTTTAGGCGAAAATGGTCGCCA
IRR : TGTAGTGGTCAACTAATCCCGGACACCGGATTAAGTTTTTCCTCGGCAAT

Insertion site


Left flankDirect repeatRight flankDR Length
AAGCGGGGTCGTCGTCATGGCCC3

DNA sequence

TGTAGTGGTCTACTAACCCCGGACACCTTTTTAGGCGAAAATGGTCGCCACACAGAGGTGTTCGATGAGCAGACAACGACGTACTTTCACCCCCGAATTC
AAGCGCGAGGCAGCCGGCCTGGTGCTCGACCAGGGCTATAGCCTTACTGAAGCCGCCCGGTCGCTCGACTTGGTTGAATCGGCGCTGCGCCGCTGGGTGA
ATCAACTCCAGTCAGAACGTGGCGGTGCTACGCCAACGAGCAAGGCGCTGACCCCCGAGCAGCAGAAGATCCAAGAGTTGGAAGCTCGGATCAACCGGCT
GGAACGGGAGAAATCGATTTTAAAAAAGGCTACCGCGCTCTTGATGGCCGAGGAACACGAGCGCTCGCGTTGATTGATCAGCTCCGCCGCGAGGAGCCTG
TCGAATTACTCTGCTCGGTGTTTGAAGTCACTCGCTCGTGTTACTACGCGCATTGCCATAAACGGCGATCTCCGGACGTTGCGCGACTGGCACTGCGCGT
TCGGGTTAACGAGCTATTTACACAGAGCCGTAGTGCCGCAGGTAGTCGAACCATCATGTGCATGATGCGCCGAGAAGGCATTCAGATCGGCCGCTTCAAG
GTGCGCAAGTTGATGCGCGAAATGAAGCTGATCAGTAAGCAACCGGGTTCACATGCCTACAAGAAGGCCACAGTAGAGCGCCCTGACATCCCGAACGTGC
TGGAGCGCGCATTCGATGTTACGGCACCGAATCAAGTATGGTGCGGCGACATCACGTATGTGTGGGCTCAAGGCCAGTGGCACTATGTGGCGGCAGTTAT
CGACCTTTTTGCACGTCGCATGGTGGGCTGGGCGTTTTCACCCAAACCGGACAGCGAATTGGTCATCAAGGCGCTGGACATGGCGTACGAGCACCGCGGG
CGGCCGCAGAATGTACTGTTTCACAGTGATCAGGGCAGCCAGTATGGCAGCCGAGCTTTTCGTCAGCGGTTGTGGCGTTACCGCTTCAAGCAAAGCATGA
GCCGCAGAGGTAATTGCCTGGACAATGCGCCTATGGAGCGTTTGTTCCGCAGCCTGAAAACGGAGTGGGTGCCAAGTGTGGGCTACATGAGTGCTGCGCA
GGCACAGCAGGACATCGGCAGGTTTCTGATGCAGCGCTACAACTGGGAGCGACCGCATCAGTTCAACGGTGGGTTACCTCCGGCGATTGCCGAGGAAAAA
CTTAATCCGGTGTCCGGGATTAGTTGACCACTACA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
309 bp102 aa65373+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSRQRRTFTPEFKREAAGLVLDQGYSLTEAARSLDLVESALRRWVNQLQSERGGATPTSKALTPEQQKIQELEARINRLEREKSILKKATALLMAEEHER
SR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1070 bp349 aa1581227+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IGAAPLGESTPVRTWRCYANEQGADPRAAEDPRVGSSDQPAGTGEIDFKKGYRALDGRGTRALALIDQLRREEPVELLCSVFEVTRSCYYAHCHKRRSPD
VARLALRVRVNELFTQSRSAAGSRTIMCMMRREGIQIGRFKVRKLMREMKLISKQPGSHAYKKATVERPDIPNVLERAFDVTAPNQVWCGDITYVWAQGQ
WHYVAAVIDLFARRMVGWAFSPKPDSELVIKALDMAYEHRGRPQNVLFHSDQGSQYGSRAFRQRLWRYRFKQSMSRRGNCLDNAPMERLFRSLKTEWVPS
VGYMSAAQAQQDIGRFLMQRYNWERPHQFNGGLPPAIAEEKLNPVSGIS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1163 bp387 aa651227+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRQRRTFTPEFKREAAGLVLDQGYSLTEAARSLDLVESALRRWVNQLQSERGGATPTSKALTPEQQKIQELEARINRLEREKSILKKGYRALDGRGTRA
LALIDQLRREEPVELLCSVFEVTRSCYYAHCHKRRSPDVARLALRVRVNELFTQSRSAAGSRTIMCMMRREGIQIGRFKVRKLMREMKLISKQPGSHAYK
KATVERPDIPNVLERAFDVTAPNQVWCGDITYVWAQGQWHYVAAVIDLFARRMVGWAFSPKPDSELVIKALDMAYEHRGRPQNVLFHSDQGSQYGSRAFR
QRLWRYRFKQSMSRRGNCLDNAPMERLFRSLKTEWVPSVGYMSAAQAQQDIGRFLMQRYNWERPHQFNGGLPPAIAEEKLNPVSGIS

 

Blast result :
Comments
ISPmo5 is 87% aa similar to ISPsy24.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Zhou,D. and Chen,F. (2019) Direct GenBank submission.