ISLmo16
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP062128.1 | ND | Listeria monocytogenes | Listeria monocytogenes Listeria monocytogenes FSL J1-208 plasmid pj1-0208 |
DNA section
IS Length : 1509 bp
Ends
IR Length : 20/24
IRL : TGAACCGACCCCCAAAAGTTAGACCAAAAATCTAACTTTTGGAGGTCGGT
IRR : TGTAGTGACCCCCAAAAGTTGGACTATTTGTAGAGTGGATTTTTTCCACT
Comments : Ends based on an empty insertion site in another strain.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| aagacccatctattctgaaa | TAA | agacagaatagatgggtctt | 3 |
DNA sequence
TGAACCGACCCCCAAAAGTTAGACCAAAAATCTAACTTTTGGAGGTCGGTATTTTTATGGCAAAATATAGTTTTGAATTTAAATTAAATGTAGTGCAAGA
ATACCTTGACAATAAAGGTGGTTATAGATATCTGGCGAAAAAACATGGTATTAAAACGAACCAACAGGTCCGAAAATGGGTGAACGCTTATCAACAATTT
GGTATAGAAGGACTTTTACGAAAACAGAAAAATAGACATTATTCTTTTCAAGCGAAGTTAAATGCGATAGAGTTATACCAAACAAGTGAGCTATCCTATC
GTGAAATTGCCAACCAAATTGGTCTCAATAATCCCGCATTAATCGCTAGTTGGATGAAAAAATTTCGGGAAGACGGAGCAGATGGGCTCTCCAAAACGAA
AGGACGACCCCCAAAAATGGCTACCCAAAAAAAGAAAAAATCTGTACAAGATAACCTAACCCAGCTTGAAAAGCTAGAAAAAGAGAATCGCATGTTAAAA
ATAGAGCTCTCTTATTTAAAGGAGTTGAGGAGGCTGCGTTTAGAGGACGAACGCAAAATGAAAGAATTGCACGAGTCATTCACAGCCTCCGACGACGCTT
CCGATTAACCGAAATTTTAGAAACCCTCCGGTTTCCTAAATCCACCTATATGTATTGGCAAAAGCATTTGAATAGAGAAAATCCAACAGAAGAAATAGAA
AAGGAGTTAAAAGCTATTCGTCAAGCACATCCTAATTACGGTTATAGACGAATGACGCAAGAACTCAAGCGTCGTGGCTTTCAAATAAATAAAAAGAAAG
TGCAACGTTTAATTCAAAAAGTGGGCTTACAAGTCAGGTCATTCACCCGTAAATCTCGGAAGTATAACTCCTATAGAGGAAATGTAGGGAAAATGGCTAA
AAATCTCGTAAAAAGACACTTTTATACTGCTATACCTCATCAAAAGATAACCACAGATACGACAGAGTTTAAATATTTTGAAACAGATTCATCTGGTATA
CTTCGTCAGAAGAAAATGTATTTAAACCCGTTTATGGATTTGTATAACAGCGAAATTATTAGCTATAATATCTCAGAACGCCCTACTGCCCAAGCAATTA
TGACTGGTCTGGAGGAGGCTATAAAGCAAACGAGTGACTGTTCTTATCGACGAACTTTCCATTCAGATCAAGGTTGGGCATATCAAATGCCTGCTTATAG
TTCCGCCTTAAAAAAACATCGTATTTTTCAAAGTATGTCTCGAAAAGGGAATTGTTTAGATAATTCTCCCATGGAGAATTTCTTCGGTCTTCTCAAACAA
GAAATATATTATGGAAAAATTTATCCTAATTACAATGAACTGAAGCAAGCTATTAAAAATTATATTCACTATTATAATCAATACCGTATGAAAGAAAAAC
TTCATTGGCAAAGCCCTATTGAATTTCGTGAAAATCAGTCACTAAAGACATAAAAATAGAGTGGAAAAAATCCACTCTACAAATAGTCCAACTTTTGGGG
GTCACTACA
ATACCTTGACAATAAAGGTGGTTATAGATATCTGGCGAAAAAACATGGTATTAAAACGAACCAACAGGTCCGAAAATGGGTGAACGCTTATCAACAATTT
GGTATAGAAGGACTTTTACGAAAACAGAAAAATAGACATTATTCTTTTCAAGCGAAGTTAAATGCGATAGAGTTATACCAAACAAGTGAGCTATCCTATC
GTGAAATTGCCAACCAAATTGGTCTCAATAATCCCGCATTAATCGCTAGTTGGATGAAAAAATTTCGGGAAGACGGAGCAGATGGGCTCTCCAAAACGAA
AGGACGACCCCCAAAAATGGCTACCCAAAAAAAGAAAAAATCTGTACAAGATAACCTAACCCAGCTTGAAAAGCTAGAAAAAGAGAATCGCATGTTAAAA
ATAGAGCTCTCTTATTTAAAGGAGTTGAGGAGGCTGCGTTTAGAGGACGAACGCAAAATGAAAGAATTGCACGAGTCATTCACAGCCTCCGACGACGCTT
CCGATTAACCGAAATTTTAGAAACCCTCCGGTTTCCTAAATCCACCTATATGTATTGGCAAAAGCATTTGAATAGAGAAAATCCAACAGAAGAAATAGAA
AAGGAGTTAAAAGCTATTCGTCAAGCACATCCTAATTACGGTTATAGACGAATGACGCAAGAACTCAAGCGTCGTGGCTTTCAAATAAATAAAAAGAAAG
TGCAACGTTTAATTCAAAAAGTGGGCTTACAAGTCAGGTCATTCACCCGTAAATCTCGGAAGTATAACTCCTATAGAGGAAATGTAGGGAAAATGGCTAA
AAATCTCGTAAAAAGACACTTTTATACTGCTATACCTCATCAAAAGATAACCACAGATACGACAGAGTTTAAATATTTTGAAACAGATTCATCTGGTATA
CTTCGTCAGAAGAAAATGTATTTAAACCCGTTTATGGATTTGTATAACAGCGAAATTATTAGCTATAATATCTCAGAACGCCCTACTGCCCAAGCAATTA
TGACTGGTCTGGAGGAGGCTATAAAGCAAACGAGTGACTGTTCTTATCGACGAACTTTCCATTCAGATCAAGGTTGGGCATATCAAATGCCTGCTTATAG
TTCCGCCTTAAAAAAACATCGTATTTTTCAAAGTATGTCTCGAAAAGGGAATTGTTTAGATAATTCTCCCATGGAGAATTTCTTCGGTCTTCTCAAACAA
GAAATATATTATGGAAAAATTTATCCTAATTACAATGAACTGAAGCAAGCTATTAAAAATTATATTCACTATTATAATCAATACCGTATGAAAGAAAAAC
TTCATTGGCAAAGCCCTATTGAATTTCGTGAAAATCAGTCACTAAAGACATAAAAATAGAGTGGAAAAAATCCACTCTACAAATAGTCCAACTTTTGGGG
GTCACTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 552 bp | 183 aa | 57 | 608 | + | No |
Description : First part of the transposase
ORF sequence :
MAKYSFEFKLNVVQEYLDNKGGYRYLAKKHGIKTNQQVRKWVNAYQQFGIEGLLRKQKNRHYSFQAKLNAIELYQTSELSYREIANQIGLNNPALIASWM
KKFREDGADGLSKTKGRPPKMATQKKKKSVQDNLTQLEKLEKENRMLKIELSYLKELRRLRLEDERKMKELHESFTASDDASD
KKFREDGADGLSKTKGRPPKMATQKKKKSVQDNLTQLEKLEKENRMLKIELSYLKELRRLRLEDERKMKELHESFTASDDASD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 327 aa | 470 | 1450 | + | No |
Description : Second part of the transposase
ORF sequence :
KARKRESHVKNRALLFKGVEEAAFRGRTQNERIARVIHSLRRRFRLTEILETLRFPKSTYMYWQKHLNRENPTEEIEKELKAIRQAHPNYGYRRMTQELK
RRGFQINKKKVQRLIQKVGLQVRSFTRKSRKYNSYRGNVGKMAKNLVKRHFYTAIPHQKITTDTTEFKYFETDSSGILRQKKMYLNPFMDLYNSEIISYN
ISERPTAQAIMTGLEEAIKQTSDCSYRRTFHSDQGWAYQMPAYSSALKKHRIFQSMSRKGNCLDNSPMENFFGLLKQEIYYGKIYPNYNELKQAIKNYIH
YYNQYRMKEKLHWQSPIEFRENQSLKT
RRGFQINKKKVQRLIQKVGLQVRSFTRKSRKYNSYRGNVGKMAKNLVKRHFYTAIPHQKITTDTTEFKYFETDSSGILRQKKMYLNPFMDLYNSEIISYN
ISERPTAQAIMTGLEEAIKQTSDCSYRRTFHSDQGWAYQMPAYSSALKKHRIFQSMSRKGNCLDNSPMENFFGLLKQEIYYGKIYPNYNELKQAIKNYIH
YYNQYRMKEKLHWQSPIEFRENQSLKT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1394 bp | 465 aa | 57 | 1450 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKYSFEFKLNVVQEYLDNKGGYRYLAKKHGIKTNQQVRKWVNAYQQFGIEGLLRKQKNRHYSFQAKLNAIELYQTSELSYREIANQIGLNNPALIASWM
KKFREDGADGLSKTKGRPPKMATQKKKKSVQDNLTQLEKLEKRESHVKNRALLFKGVEEAAFRGRTQNERIARVIHSLRRRFRLTEILETLRFPKSTYMY
WQKHLNRENPTEEIEKELKAIRQAHPNYGYRRMTQELKRRGFQINKKKVQRLIQKVGLQVRSFTRKSRKYNSYRGNVGKMAKNLVKRHFYTAIPHQKITT
DTTEFKYFETDSSGILRQKKMYLNPFMDLYNSEIISYNISERPTAQAIMTGLEEAIKQTSDCSYRRTFHSDQGWAYQMPAYSSALKKHRIFQSMSRKGNC
LDNSPMENFFGLLKQEIYYGKIYPNYNELKQAIKNYIHYYNQYRMKEKLHWQSPIEFRENQSLKT
KKFREDGADGLSKTKGRPPKMATQKKKKSVQDNLTQLEKLEKRESHVKNRALLFKGVEEAAFRGRTQNERIARVIHSLRRRFRLTEILETLRFPKSTYMY
WQKHLNRENPTEEIEKELKAIRQAHPNYGYRRMTQELKRRGFQINKKKVQRLIQKVGLQVRSFTRKSRKYNSYRGNVGKMAKNLVKRHFYTAIPHQKITT
DTTEFKYFETDSSGILRQKKMYLNPFMDLYNSEIISYNISERPTAQAIMTGLEEAIKQTSDCSYRRTFHSDQGWAYQMPAYSSALKKHRIFQSMSRKGNC
LDNSPMENFFGLLKQEIYYGKIYPNYNELKQAIKNYIHYYNQYRMKEKLHWQSPIEFRENQSLKT
Blast result :
Comments
ISLmo11 is 79% aa (transposase) similar to ISLmo11.
ISLmo11 was identified by in silico sequence analysis of Listeria monocytogenes FSL J1-208 plasmid pj1-0208.
The third ORF is the putative transposase reconstructed in silico by possible -1 frameshift.
ISLmo11 was identified by in silico sequence analysis of Listeria monocytogenes FSL J1-208 plasmid pj1-0208.
The third ORF is the putative transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska, C. and Bartosik, D. (2021) Direct submission.