ISH4
- Family IS1595
- Group ISH4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF016485 | ND | Halobacterium sp. | Halobacterium salinarum R1 Halobacterium sp. NRC-1 plasmid pNRC100 Halobacterium salinarum R1 plasmid pHS4 Halobacterium salinarum R1 plasmid pHS1 |
DNA section
IS Length : 1004 bp
Ends
IR Length : 23/29
IRL : CGCTCTTGGGTAGAGGATTGTTGGTCAGTTCTCAGCAGTAGCTGGAAGTC
IRR : CGCTCTTGTGGCGGAGATTGTTGGTGAGTCATAGCGCAGTCTCAAGGATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATGTCGATA | AGATTAGT | TAATAGTGTG | 8 |
| GACCGCGACA | TACCAAATT | AGTGTACGCA | 9 |
| TACAATCTTA | ACTATCTA | TTAAATCACA | 8 |
DNA sequence
CGCTCTTGGGTAGAGGATTGTTGGTCAGTTCTCAGCAGTAGCTGGAAGTCACAGCAGGCAAGGGTGACGCGGCCGCGTCACCCGACGAACGATGATGCCA
ATCAAGACATTCGTCTCGGAGCGTCGCGCCGCGAATCTGCTAGCACAGATTCGCTGGCGTAACGGCGTCTATTGCCCGAGCTGCCGTGCCGAATCTGTGA
TTCGGTACGGCAGCTATCGCGTGTTTCAGCGGTATCTGTGTAAGGATTGCGACCGCACGTTCAACGATCAGACTGGCACCGTCTTCGAACACTCTGCGGT
CGCGCTCAGAAAGTGGTTTCTCGCCGTCTACACCTACATCCGCTTAAACACGAGTATCAGGCAGTTAGACGCAGAGATCGACGTTTCCTACAAGACGGTC
TACCGGCGCGTCCAGCGCTTCCTGCGAGCGCTGGACGCGCCTCGACCACACCTCGAAGGCCCCGTTGAGATCGACGAATTCTACGTGAAAGCGGGCCTCA
AGGGCCGCGAGCGCGACCAACCGTCGCGCTCGCGTGGACTGTCCACGCGCGGACGTGGAACATACGCTGAGGATAAGCTCCCTGTGTTTGTTCTCGCAGA
TCGTGGCACCGGAGAACGGCACGTGATCCCGGCGAAAGCCGCGACCGAATCGCGGATTCGACTCCTGCTGGCCGACCGACAGCAGGAGTCGTTAACCGTC
TACACAGACGGCTTTCGGGCGTACGATCCACTTGACGAGGACGACGCTTTCACCCGTGAATACGTCGTCCACGGTGACGGCGAGTACGTCAATGGAGACG
TTCATGTGAACACCTGCGAGAGCCACGCGTCGCTGGCGCGACGGTGGCTCTCGCCGCACCGAGGCGTCTCTAAAGACAGACTCACACCATATCTCCGGGC
GTTTCAGCTCCGACGTGAAGTGTTCCGAAAACCGGGGAAAGAAGCGCTCAAAACGATCCTTGAGACTGCGCTATGACTCACCAACAATCTCCGCCACAAG
AGCG
ATCAAGACATTCGTCTCGGAGCGTCGCGCCGCGAATCTGCTAGCACAGATTCGCTGGCGTAACGGCGTCTATTGCCCGAGCTGCCGTGCCGAATCTGTGA
TTCGGTACGGCAGCTATCGCGTGTTTCAGCGGTATCTGTGTAAGGATTGCGACCGCACGTTCAACGATCAGACTGGCACCGTCTTCGAACACTCTGCGGT
CGCGCTCAGAAAGTGGTTTCTCGCCGTCTACACCTACATCCGCTTAAACACGAGTATCAGGCAGTTAGACGCAGAGATCGACGTTTCCTACAAGACGGTC
TACCGGCGCGTCCAGCGCTTCCTGCGAGCGCTGGACGCGCCTCGACCACACCTCGAAGGCCCCGTTGAGATCGACGAATTCTACGTGAAAGCGGGCCTCA
AGGGCCGCGAGCGCGACCAACCGTCGCGCTCGCGTGGACTGTCCACGCGCGGACGTGGAACATACGCTGAGGATAAGCTCCCTGTGTTTGTTCTCGCAGA
TCGTGGCACCGGAGAACGGCACGTGATCCCGGCGAAAGCCGCGACCGAATCGCGGATTCGACTCCTGCTGGCCGACCGACAGCAGGAGTCGTTAACCGTC
TACACAGACGGCTTTCGGGCGTACGATCCACTTGACGAGGACGACGCTTTCACCCGTGAATACGTCGTCCACGGTGACGGCGAGTACGTCAATGGAGACG
TTCATGTGAACACCTGCGAGAGCCACGCGTCGCTGGCGCGACGGTGGCTCTCGCCGCACCGAGGCGTCTCTAAAGACAGACTCACACCATATCTCCGGGC
GTTTCAGCTCCGACGTGAAGTGTTCCGAAAACCGGGGAAAGAAGCGCTCAAAACGATCCTTGAGACTGCGCTATGACTCACCAACAATCTCCGCCACAAG
AGCG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 885 bp | 294 aa | 91 | 975 | + | No |
Chemistry : DDE
ORF sequence :
MMPIKTFVSERRAANLLAQIRWRNGVYCPSCRAESVIRYGSYRVFQRYLCKDCDRTFNDQTGTVFEHSAVALRKWFLAVYTYIRLNTSIRQLDAEIDVSY
KTVYRRVQRFLRALDAPRPHLEGPVEIDEFYVKAGLKGRERDQPSRSRGLSTRGRGTYAEDKLPVFVLADRGTGERHVIPAKAATESRIRLLLADRQQES
LTVYTDGFRAYDPLDEDDAFTREYVVHGDGEYVNGDVHVNTCESHASLARRWLSPHRGVSKDRLTPYLRAFQLRREVFRKPGKEALKTILETAL
KTVYRRVQRFLRALDAPRPHLEGPVEIDEFYVKAGLKGRERDQPSRSRGLSTRGRGTYAEDKLPVFVLADRGTGERHVIPAKAATESRIRLLLADRQQES
LTVYTDGFRAYDPLDEDDAFTREYVVHGDGEYVNGDVHVNTCESHASLARRWLSPHRGVSKDRLTPYLRAFQLRREVFRKPGKEALKTILETAL
Blast result :
Comments
One copie was present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 122729-123732.
ISH4 is 89% aa similar to ISH50 (ISH4 is longer than ISH50)
ISH4 is 89% aa similar to ISH50 (ISH4 is longer than ISH50)
References
1] Ng W.V., Ciufo S.A., Smith T.M., Bumgarner R.E., Baskin D., Faust J., Hall B., Loretz C., Seto J., Slagel J., Hood L., and DasSarma S. (1998) Genome Res. 8, 1131-1141
2] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91: 335-346
2] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91: 335-346