ISMahy8
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008740.1 | ND | Marinobacter hydrocarbonoclasticus | Marinobacter hydrocarbonoclasticus VT8 |
DNA section
IS Length : 2640 bp
Ends
IR Length : 37/48
IRL : TGCATATTCCGGCCCATCGTGACCGCCCATTCCGTTTGAACGTGACCGCC
IRR : TGCGTATTCCGGCGAACGTGACCGGTCATTCCGGGCATCGTGACCGATTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGAAGATTTAGGT | CTTAC | AGAATATACACC | 5 |
DNA sequence
TGCATATTCCGGCCCATCGTGACCGCCCATTCCGTTTGAACGTGACCGCCTGTTCCGGATGATCGTGACCGCTCATTCCGTTTTATCGTGACCGATTTCG
GGTAGTTTTCCGGAATCGCCGGTCACGATGCCGGAATCATCGGTCACGTTCCTCCGGAATCCCTTCCAACACGCTGGAATTCTTCAACTTTATCCGGCAT
ACCCCTTCCTTTTTCATCACGAGGAAGGGCGGATGCCGGCAGCGAGGATTTCCATGCGACAAATCATCGAGGTCTTGCGCCTCAAGTACGAAGCGGGCCT
GAGCCATGAGCGCATTGCCCGCGCCTGTGGCCTCTCCAAGGGCGTAGTCGGTAAATACGTCAGCCTGGCCACCGCTCAGGGCATCACCTGGCCGTTGCCA
GAAGGCACGGACGAAGCTCGGCTGGAAGCCCAGCTGTTCCCGGCCAAGACGCCGCCTGCACGCTTTGCCGAGCCCGACTACTTTCAGGTCCATCAGGAGC
TGAAGACCAAAGGCGTCACCCTGCAACTGCTCTGGGCTGAGTACGTGGAACGTCACGGCGACAAGGCCCGCCGGTACAGCCAGTTCTGCCATCACTACCG
GCTCTGGCAAGGCCGTCAGCGGCGCAGCATGCGCCAGGTCCACCGGGCCGGTGAGAAGATCTTTATCGACTACTGCGGCCCCACGGTTCCGGTGGTGGAT
CGCAGCACTGGTGAGATGCGCAAGGCTCAGGTCTTTGTCGCTGTGCTGGGCGCCTCCAGCTACACCTTCGCCGAGGCCACGTGGAGCCAGTCTCTACCGG
ACTGGATCGCCTCCCACCAGCGGATGCTGGCGTTCTACGGTGGCGTGCCGGAACTGCTGGTTCCGGACAACCTCAAGGCAGCAGTCACCAAGGCGGACCG
ATACACGCCTCAGATCAACGAGACCTACGCGGAACTGGCGGCCCATTACCAGACGGCGGTACTGCCGGCACGTCCCTACAAGCCCAAGGACAAGGCGAAG
GCCGAAGCCGCTGTGCTGCTGGTTGAACGCTGGATCCTGGCCCGGTTACGACACCGGACGTTCTTCTCACTGGCCGAACTGAACTCGGCGATTGCAGACC
TGCTACCAGCACTGAACCAGCGCCCGTTCCAGGGGCGCTCCGAGAGCCGCCAGAGTCTGTTCGAGGCACTGGACCGACCCGCACTCAGGCCACTGCCTGC
CACGCCCTATGTCTATGCTGAGTGGCGCAAGGCACGACCGGGTATCGACTACCACATCGAGATCGACAAACGCCTGTACAGCGTGCCTCACGCCCTGGTG
GGGGTGAAGCTGGATGTTCGGGTGACCGATACGTCCGTTGAGGTCATGCACAAAGGCCAGCGGGTGGCTCTGCACCCACGGCATGGCAAAAGCCGCTTCG
TGACCCTGACCGAGCACATGCCCAAGTCCCACCAGGCCCATCAGAACTGGTCTCCCGGGCGGTTCCTGAACTGGGCCACGGACATCGGGCCGGCCACACT
CGACGTGGTACAGCGACAGCTTAAGGATCGCCCTCATCCAGAGCATGGTTACCGAGCATGCCTGGGTCTGCTGAACCTCAGTCGCCGCTACAGCCGTGAC
CGCCTGGAGCAGGCTTGTAGCCGAGCACTGGCCATCAACTCGGCCAGCTATCAGAGCATCAGCTCAATCCTGAAGCAGGGGCTGGATCAACTGCCTCTTC
CCCTGACCGAGGAAGATCCGGAGCTGGCCGATCTGCCCTTACACACCAACGTTCGCGGCCCCCGCTACTACCACTGATCCCGGAGAAACTGATGTTGACT
CAAAACACCCTCGATACCCTGCGACAGCTCAAGCTGACAGGCATGTGCGATGCCTTGGAACAACAGCGGGCTCAGCCCGACACCCACGACCTGGCGTTCG
AGGAGCGCTTGGCCCTGCTGGTAGATCGCGAAGTGCTGCACCGTGAAAACCGGCGATTGGAGCGGCTGCTCAAGGCCGCCCGTCTGCGTGTGCCGGCCTG
TATTGAAGACATCGACTACCACCACCCGAGAGGGCTGGAGCGTTCTCGCATGGCCGGACTGGCCAGCTGTGACTGGATCCGCCAATCCCTGAACCTGTGC
ATTACCGGTCCCACCGGGTGCGGTAAGACCTGGCTGGCGTGTGCCCTGGGCAATCAGGCCTGCCGGCAAGGCCTCTCAGTTCGCTACCTACGGGTACCGC
GCCTGTTCGAACAGCTGCGCATCGCCCATGGCGATGGCAGCTATGCCCGACTGATGAACCAGTTGCTAAAAACCGACCTGCTGATCCTGGATGACTGGGG
GATGCAGAAGGTGACAGCGCAACAACGGCAAGATCTGATGGAGGTGATCGAAGACCGACATGGGAGAGGCTCAACGCTCATCGCCAGTCAGCTACCCACG
GAACACTGGCACGACTATATCGGTTCCGCCACGCTCGCCGACGCCATCTTGGACCGGCTCCTGCACGGCGCTCACCGGCTTAATCTGAAGGGGGAATCAC
TGCGAAAAGCGAAAACACAAAAGACGGAATCGGTTGACCCATCGTGACCGCTCAGAGTACAAAACCGACTCCAGCGTGATGGCCGTGTGCAAATCGGTCA
CGATGCCCGGAATGACCGGTCACGTTCGCCGGAATACGCA
GGTAGTTTTCCGGAATCGCCGGTCACGATGCCGGAATCATCGGTCACGTTCCTCCGGAATCCCTTCCAACACGCTGGAATTCTTCAACTTTATCCGGCAT
ACCCCTTCCTTTTTCATCACGAGGAAGGGCGGATGCCGGCAGCGAGGATTTCCATGCGACAAATCATCGAGGTCTTGCGCCTCAAGTACGAAGCGGGCCT
GAGCCATGAGCGCATTGCCCGCGCCTGTGGCCTCTCCAAGGGCGTAGTCGGTAAATACGTCAGCCTGGCCACCGCTCAGGGCATCACCTGGCCGTTGCCA
GAAGGCACGGACGAAGCTCGGCTGGAAGCCCAGCTGTTCCCGGCCAAGACGCCGCCTGCACGCTTTGCCGAGCCCGACTACTTTCAGGTCCATCAGGAGC
TGAAGACCAAAGGCGTCACCCTGCAACTGCTCTGGGCTGAGTACGTGGAACGTCACGGCGACAAGGCCCGCCGGTACAGCCAGTTCTGCCATCACTACCG
GCTCTGGCAAGGCCGTCAGCGGCGCAGCATGCGCCAGGTCCACCGGGCCGGTGAGAAGATCTTTATCGACTACTGCGGCCCCACGGTTCCGGTGGTGGAT
CGCAGCACTGGTGAGATGCGCAAGGCTCAGGTCTTTGTCGCTGTGCTGGGCGCCTCCAGCTACACCTTCGCCGAGGCCACGTGGAGCCAGTCTCTACCGG
ACTGGATCGCCTCCCACCAGCGGATGCTGGCGTTCTACGGTGGCGTGCCGGAACTGCTGGTTCCGGACAACCTCAAGGCAGCAGTCACCAAGGCGGACCG
ATACACGCCTCAGATCAACGAGACCTACGCGGAACTGGCGGCCCATTACCAGACGGCGGTACTGCCGGCACGTCCCTACAAGCCCAAGGACAAGGCGAAG
GCCGAAGCCGCTGTGCTGCTGGTTGAACGCTGGATCCTGGCCCGGTTACGACACCGGACGTTCTTCTCACTGGCCGAACTGAACTCGGCGATTGCAGACC
TGCTACCAGCACTGAACCAGCGCCCGTTCCAGGGGCGCTCCGAGAGCCGCCAGAGTCTGTTCGAGGCACTGGACCGACCCGCACTCAGGCCACTGCCTGC
CACGCCCTATGTCTATGCTGAGTGGCGCAAGGCACGACCGGGTATCGACTACCACATCGAGATCGACAAACGCCTGTACAGCGTGCCTCACGCCCTGGTG
GGGGTGAAGCTGGATGTTCGGGTGACCGATACGTCCGTTGAGGTCATGCACAAAGGCCAGCGGGTGGCTCTGCACCCACGGCATGGCAAAAGCCGCTTCG
TGACCCTGACCGAGCACATGCCCAAGTCCCACCAGGCCCATCAGAACTGGTCTCCCGGGCGGTTCCTGAACTGGGCCACGGACATCGGGCCGGCCACACT
CGACGTGGTACAGCGACAGCTTAAGGATCGCCCTCATCCAGAGCATGGTTACCGAGCATGCCTGGGTCTGCTGAACCTCAGTCGCCGCTACAGCCGTGAC
CGCCTGGAGCAGGCTTGTAGCCGAGCACTGGCCATCAACTCGGCCAGCTATCAGAGCATCAGCTCAATCCTGAAGCAGGGGCTGGATCAACTGCCTCTTC
CCCTGACCGAGGAAGATCCGGAGCTGGCCGATCTGCCCTTACACACCAACGTTCGCGGCCCCCGCTACTACCACTGATCCCGGAGAAACTGATGTTGACT
CAAAACACCCTCGATACCCTGCGACAGCTCAAGCTGACAGGCATGTGCGATGCCTTGGAACAACAGCGGGCTCAGCCCGACACCCACGACCTGGCGTTCG
AGGAGCGCTTGGCCCTGCTGGTAGATCGCGAAGTGCTGCACCGTGAAAACCGGCGATTGGAGCGGCTGCTCAAGGCCGCCCGTCTGCGTGTGCCGGCCTG
TATTGAAGACATCGACTACCACCACCCGAGAGGGCTGGAGCGTTCTCGCATGGCCGGACTGGCCAGCTGTGACTGGATCCGCCAATCCCTGAACCTGTGC
ATTACCGGTCCCACCGGGTGCGGTAAGACCTGGCTGGCGTGTGCCCTGGGCAATCAGGCCTGCCGGCAAGGCCTCTCAGTTCGCTACCTACGGGTACCGC
GCCTGTTCGAACAGCTGCGCATCGCCCATGGCGATGGCAGCTATGCCCGACTGATGAACCAGTTGCTAAAAACCGACCTGCTGATCCTGGATGACTGGGG
GATGCAGAAGGTGACAGCGCAACAACGGCAAGATCTGATGGAGGTGATCGAAGACCGACATGGGAGAGGCTCAACGCTCATCGCCAGTCAGCTACCCACG
GAACACTGGCACGACTATATCGGTTCCGCCACGCTCGCCGACGCCATCTTGGACCGGCTCCTGCACGGCGCTCACCGGCTTAATCTGAAGGGGGAATCAC
TGCGAAAAGCGAAAACACAAAAGACGGAATCGGTTGACCCATCGTGACCGCTCAGAGTACAAAACCGACTCCAGCGTGATGGCCGTGTGCAAATCGGTCA
CGATGCCCGGAATGACCGGTCACGTTCGCCGGAATACGCA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1545 bp | 514 aa | 233 | 1777 | + | No |
Chemistry : DDE
ORF sequence :
MPAARISMRQIIEVLRLKYEAGLSHERIARACGLSKGVVGKYVSLATAQGITWPLPEGTDEARLEAQLFPAKTPPARFAEPDYFQVHQELKTKGVTLQLL
WAEYVERHGDKARRYSQFCHHYRLWQGRQRRSMRQVHRAGEKIFIDYCGPTVPVVDRSTGEMRKAQVFVAVLGASSYTFAEATWSQSLPDWIASHQRMLA
FYGGVPELLVPDNLKAAVTKADRYTPQINETYAELAAHYQTAVLPARPYKPKDKAKAEAAVLLVERWILARLRHRTFFSLAELNSAIADLLPALNQRPFQ
GRSESRQSLFEALDRPALRPLPATPYVYAEWRKARPGIDYHIEIDKRLYSVPHALVGVKLDVRVTDTSVEVMHKGQRVALHPRHGKSRFVTLTEHMPKSH
QAHQNWSPGRFLNWATDIGPATLDVVQRQLKDRPHPEHGYRACLGLLNLSRRYSRDRLEQACSRALAINSASYQSISSILKQGLDQLPLPLTEEDPELAD
LPLHTNVRGPRYYH
WAEYVERHGDKARRYSQFCHHYRLWQGRQRRSMRQVHRAGEKIFIDYCGPTVPVVDRSTGEMRKAQVFVAVLGASSYTFAEATWSQSLPDWIASHQRMLA
FYGGVPELLVPDNLKAAVTKADRYTPQINETYAELAAHYQTAVLPARPYKPKDKAKAEAAVLLVERWILARLRHRTFFSLAELNSAIADLLPALNQRPFQ
GRSESRQSLFEALDRPALRPLPATPYVYAEWRKARPGIDYHIEIDKRLYSVPHALVGVKLDVRVTDTSVEVMHKGQRVALHPRHGKSRFVTLTEHMPKSH
QAHQNWSPGRFLNWATDIGPATLDVVQRQLKDRPHPEHGYRACLGLLNLSRRYSRDRLEQACSRALAINSASYQSISSILKQGLDQLPLPLTEEDPELAD
LPLHTNVRGPRYYH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 756 bp | 251 aa | 1792 | 2547 | + | No |
AG : IS21 helper
ORF sequence :
MLTQNTLDTLRQLKLTGMCDALEQQRAQPDTHDLAFEERLALLVDREVLHRENRRLERLLKAARLRVPACIEDIDYHHPRGLERSRMAGLASCDWIRQSL
NLCITGPTGCGKTWLACALGNQACRQGLSVRYLRVPRLFEQLRIAHGDGSYARLMNQLLKTDLLILDDWGMQKVTAQQRQDLMEVIEDRHGRGSTLIASQ
LPTEHWHDYIGSATLADAILDRLLHGAHRLNLKGESLRKAKTQKTESVDPS
NLCITGPTGCGKTWLACALGNQACRQGLSVRYLRVPRLFEQLRIAHGDGSYARLMNQLLKTDLLILDDWGMQKVTAQQRQDLMEVIEDRHGRGSTLIASQ
LPTEHWHDYIGSATLADAILDRLLHGAHRLNLKGESLRKAKTQKTESVDPS
Blast result :
Comments
ISMahy8 is 78% aa (transposase) similar to ISNarch3.
References
1] Sarah Sonbol (2020) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Edwards,K. and Richardson,P. (200-) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Edwards,K. and Richardson,P. (200-) Direct submission GenBank.