ISfinder Mirror Site

ISGpa1

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP069308	ND	Glaesserella parasuis	Glaesserella parasuis EHP1804

DNA section

IS Length : 1315 bp

Ends

IR Length : 27/35

IRL : GAGCCTCTCCCACAATTTGTGTAAATACCTGTTTCTGAGATAATACATTC
IRR : GACCCTATCCCAAATTCTGTGTAAACACCCATTTCAACTTAACGGTGATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCATCCGAAAGG	ATGCCGTT	TTTTATTTCCCT	8
ATTGACGATTTA	AAAATCAT	AGCTGAAAGAAT	8
TATTTAGTTTAATATTTTAA		CAATTAAGTGATCCTTTCCT	0

DNA sequence

GAGCCTCTCCCACAATTTGTGTAAATACCTGTTTCTGAGATAATACATTCAGACACAGGTATTTTATTATGAACGAAAAACAACTTCACGCCTTGGCAGC
GGAATTTGCCAAAAACCTAAAAACACCAGAAGACCTCAATCAATTTTCACGGATGCTCAAGAAAATCACCGTCGAGGCTGCGTTAAATGGTGAACTGACC
GACCATCTTGGTTATGAAAAACATCAGCCTAGAAAAGGTAAAAATGCACGTAACGGTTACACATCTAAGACCGTCATTTGTGATGAAGGTGAGATAGAAA
TTGAGACGCCTCGTGACCGTGACGGCACCTTTGAACCGCAACTTATCAAGAAAAACCAAACCCGCATCACAGGAATGGATGAGCAGATTATTGCCTTATA
TTCCAAAGGGTTAAGTAATCAGGAAATCGTTGAAATGTTCAAAGAACTCTATGATGCGGATGTGTCAAGCAGCCTGATTTCTCGCGTTACCGACGCCGTG
AAAGAACGCGTAATGGAATGGCAAAATCGCCCACTTGATGCGGTTTATCCAATTGTTTACCTAGATTGTATCGTAGTGAAAGTACGCCAAGATGGACGAA
TTATCAACAAATCCGTGTTTGTTGCCTTGGGTGTGAATCTTGAAGGACATAAAGAGTTATTGGGGCTTTGGATTGCTGAAAATGAAGGTGCGAAGTTCTG
GGCGAATGTGCTGACAGAGCTTCAAAATCGAGGCTTAAAAGACATTTTTATTGCCTGTGTAGACGGTTTAAAAGGCTTCCCGGAAGCCATCAATGCAGTC
TATCCTAAAACGAAGATTCAGCTTTGCATTGTGCATTTAGTGCGTAACAGCTTGAAATTCGTTTCGTGGAAAGATTACAAAGCCGTCACCGCAGATTTAA
AGCAGGTTTATCAAGCCCCGACGGAAGCACAGGCTCGCGAAAATCTGACCGCACTTTCGCAAAAATGGCAGGCAAAATACCCGCTTGTGGCGAAAGGCTG
GGAAGATAACTGGGCAAATATTGCCACATTTTTTGATTATCCAGCTGATATTCGTAAAGCGATTTATACCACGAATGCTGTGGAATCGCTTAATAGCGTG
ATTCGTCGCGTGATTAAAAAACGAAATGTATTCCCGACGGATGATTCAGTTTTCAAAGTGATTTGGCTTGCGATTAAAGATGCATCAAAAAAATGGACAA
TGCCGATTCAGAACTGGAAACTGGCGATGAATCGATTTATGATTGATTTTGGTGATCGCTTAGACGATCACCGTTAAGTTGAAATGGGTGTTTACACAGA
ATTTGGGATAGGGTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1209 bp	402 aa	69	1277	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNEKQLHALAAEFAKNLKTPEDLNQFSRMLKKITVEAALNGELTDHLGYEKHQPRKGKNARNGYTSKTVICDEGEIEIETPRDRDGTFEPQLIKKNQTRI
TGMDEQIIALYSKGLSNQEIVEMFKELYDADVSSSLISRVTDAVKERVMEWQNRPLDAVYPIVYLDCIVVKVRQDGRIINKSVFVALGVNLEGHKELLGL
WIAENEGAKFWANVLTELQNRGLKDIFIACVDGLKGFPEAINAVYPKTKIQLCIVHLVRNSLKFVSWKDYKAVTADLKQVYQAPTEAQARENLTALSQKW
QAKYPLVAKGWEDNWANIATFFDYPADIRKAIYTTNAVESLNSVIRRVIKKRNVFPTDDSVFKVIWLAIKDASKKWTMPIQNWKLAMNRFMIDFGDRLDD
HR

Blast result :

Comments

ISGpa1 is 87% aa similar to IS1414.

References

1]] Eaves-Pyles,T., Allen,C.A., Taormina,J., Swidsinski,A., Tutt,C.B., Jezek,G.E., Islas-Islas,M. and Torres,A.G. (2008) Int. J. Med. Microbiol. 298 (5-6), 397-409.
2] Nash,J.H., Villegas,A., Kropinski,A.M., Aguilar-Valenzuela,R., Konczy,P., Mascarenhas,M., Ziebell,K., Torres,A.G., Karmali,M.A. and Coombes,B.K. (2010) BMC Genomics 11 (1), 667.