ISAdh3

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Aeromonas dhakensis
Aeromonas dhakensis
DNA section
IS Length : 1235 bp

Ends


IR Length : 26/30

IRL : TGAATCGCCCCCACTTTCCTAGACACCTCAAGAGCCTCAAACTGAGGTCA
IRR : TGAATCGCCCCGGCTTCTCTAGACACTTTTGAGCCGTTCGAAATATTGCT

Insertion site


Left flankDirect repeatRight flankDR Length
TCTGAAGGTGTGAATCGCCCCCACTTTCCTAGACACCTGTGAAGTGTCTAGAGAAGCCGGGGCGATTCAGTGTTGATGT3

DNA sequence

TGAATCGCCCCCACTTTCCTAGACACCTCAAGAGCCTCAAACTGAGGTCATTCAGGAGGTGTTATGAGCAAGCAGCAACGTTACACGGAAGAATTCAAAC
TGGCCGCCGTCAAGCAAGTGACTGAGCGCCATTACCCAGTGGCAGAGGTCGCTAAGCGACTTGGTGTTTCCAGCCACAGTCTTTACGCCTGGATCAAACG
CTACAGCCAGCCCGAAGTGCAGCAACAGCATGACTCCAGCCAGCAAGCCGAAATTCGGCGCCTCAAGGCTGAACTGCGCCGCGTCACCGATGAGCGAGAT
ATCTTAAAAAAGGCCGCCGCGTACTTTGCCAAGCTGTCAGGTTAAGGTACGCCTTTATCCGGGAACACGAGTCCACGCATCCTGTCCGCAGACTGTGCCG
GATGATGAATGTCCACCCGAGCGGTTATTACGCTTGGCAACATCAGCCCTTATCTACCCGAGCACAGGAGGATCAACGCCTCCTGGGCCAGATCAAGCAA
TGTTGGCTAGAAAGCGGGGGCGTCTATGGCTATCGCAAGATCCGGGATGACCTGCGAGAAATGGGCGAGACCTGTGGCAAGCACCGGGTGGCGCGATTAA
TGCGACATGAAGGGTTACGTTCACAAACCGGCTATCACCGCCGTCCAGGCCATTATGGTGGCCGGCCAACGGTGGTTGCACCGAATCATTTGGAACGCCA
GTTCGATGTGACCGAGCCCAATAAGGTCTGGGTAACGGACATCACCTACATTCGCACGCATGAAGGCTGGCTCTATCTGGCTGTGGTATTGGATTTGTTC
TCACGACACGTCATTGGTTGGTCGATGGGGCAGCGGATCGACCGAGAGCTGGCCATCAGTGCGTTACTGATGGCGGTGTGGCGACGGCGACCCGAACAAG
AAGTGCTGGTTCATTCCGACCAAGGGAGCCAGTTTAGCAGCTATCACTGGCAGGATTTTTTGCGGGAAAACCAGCTCAAGGCCAGCATGAGTAGGCGCGG
CAATTGCCATGATAACGCGGTGGCGGAGAGTTTCTTCCAGCTCCTCAAACGCGAGCGGATCCGACGTAGAACCTATGCGGATCGCGAGGAAGCCCGCCAG
GATGTGTTCGATTACATTGAAATGTTTTACAACCCCAAGCGCCGGCACAGTTTCAACAATGGGCTATCGCCAGTAGAGTGTGAAAAGCAATATTTCGAAC
GGCTCAAAAGTGTCTAGAGAAGCCGGGGCGATTCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
282 bp92 aa64345+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSKQQRYTEEFKLAAVKQTERHYPVAEVAKRLGVSSHSLYAWIKRYSQPEVQQQHDSSQQAEIRRLKAELRRVTDERDILKKAAAYFAKLSG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
924 bp288 aa2941217+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ARYLKKGRRVLCQAVRLRYAFIREHESTHPVRRLCRMMNVHPSGYYAWQHQPLSTRAQEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVA
RLMRHEGLRSQTGYHRRPGHYGGRPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAVVLDLFSRHVIGWSMGQRIDRELAISALLMAVWRRRP
EQEVLVHSDQGSQFSSYHWQDFLRENQLKASMSRRGNCHDNAADREEARQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1154 bp364 aa641217+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKQQRYTEEFKLAAVKQTERHYPVAEVAKRLGVSSHSLYAWIKRYSQPEVQQQHDSSQQAEIRRLKAELRRVTDERDILKKGRRVLCQAVRLRYAFIRE
HESTHPVRRLCRMMNVHPSGYYAWQHQPLSTRAQEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVARLMRHEGLRSQTGYHRRPGHYGGR
PTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAVVLDLFSRHVIGWSMGQRIDRELAISALLMAVWRRRPEQEVLVHSDQGSQFSSYHWQDFLR
ENQLKASMSRRGNCHDNAADREEARQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV

 

Blast result :
Comments
ISAdh3 is 92% (transposase) aa similar to ISAeca4. The third ORF is the putative ORFAB transposase reconstructed in silico by posible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.