ISSeq3
- Family IS3
- Group IS150
Isoform Synonym(s) ISSzo3
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012471 | ND | Streptococcus equi | Streptococcus equi subsp. equi 4047 Streptococcus equi subsp. zooepidemicus H70 Streptococcus equi subsp. zooepidemicus MGCS10565 Streptococcus equi subsp. zooepidemicus ATCC 35246 |
DNA section
IS Length : 1430 bp
Ends
IR Length : 23/26
IRL : TGAACTGCCCCCCAAAAGTTAGACATAAAATCTAACAATTGGGGGGCTAT
IRR : TGTACTGACCCCCCAAAAGTTGGACACGACATATTAGTGAAAGGATTTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATCCCTAAG | CTGGTCCTCT | 0 |
DNA sequence
TGAACTGCCCCCCAAAAGTTAGACATAAAATCTAACAATTGGGGGGCTATTTTTATGACATTGAGTTATGAAGACAAGGTTCAAATCTATGAGCTACGGC
ACATTGGAAAGTCCATTAAATGCTTATCAGAAAAGTTTAGTATTGCAGAATCTGACCTCAAATACATGATTCGCCTGATTGACAGGTATGGGTTAGCCAT
TGTCCAAAAAGGTAAGAATAGTTATTATTCTCCAGAACTGAAGCAAGAGATAATAGATAAAGTTCTGATTGATGGTCAATCTCAAAAACAGACGTCCTTA
GACTATGCTTTACCAAATTCTAGTATGCTTTCAAGGTGGATAGCGCAATACAAGAAAAACGGCTATACTATTCTTGAGAAAAGAAGAGGGAGGCCACCAA
AGATGGGACGTCAACCAAAGAAGACTTTAGAACAAATGACAGAGTTGGAGCGACTCCAAAAAGAATTAGACTACCTTAGAGCGGAGAATGCTGTGCTAAA
AAAGCTGAGAGAATACCGGTTGAGGGACGAAGCAAAGCTCAAAGAGCAACAGAAATCATCCAAGAATTAATCGGTCAATTTTCTCTAGCAACTTTGCTTG
AAATCCTTGATTTATCGCGGTCAACCTATTATTATCAAGTCAAGCAACTAGCTCAAGAAGATAAGGACATGGACTTAAAGGAGCTCATTCAAGGCATCTA
TGATGAACATCATGGCAATTATGGCTATCGTCGCATTCATCTGGAACTAAGAAATCGTGGTTTTATCGTCAATCACAAAAAAGTACAACGTTTGATGACT
GTCATGGGCTTAAAAGCTCGTATCCGTCGTAAGCGCAAGTATTCTTCTTACAAAGGTGAGGTTGGCAAAAAGGCTGATAATCTGATTAAACGTCAGTTTG
AAGGTTCTAAGCCCTACGAGAAGTGCTATACCGATGTGACGGAATTTACCTTACCTGAGGGGAAACTCTATCTATCGCCTGTTCTTGACGGCTATAACAG
TGAGATTATTGATTTCACCCTGTCTCGATCGCCTGACTTGAAGCAAGTACAAACCATGCTTGAGAAGGCTTTTCCAGCGGATTCGTACAATGGAACGATT
CTCCACAGCGATCAAGGCTGGCAATATCAACATCAGTCTTATCATCACTTTTTGGAGACTAAAGGCATTCGTCCATCCATGTCTCGCAAGGGAAATAGTC
CAGATAATGGGATGATGGAGTCCTTCTTTGGTATTCTCAAATCTGAGATGTTTTACGGCCTTGAGACAACTTATCAATCCCTTAATGAGCTTGAACAAGC
TATTACAGATTACATTTTTTACTACAACAACAAACGCATTAAAGCAAAGCTAAAAGGACTTAGCCCTGTGCAATACAGAACTAAATCCTTTCACTAATAT
GTCGTGTCCAACTTTTGGGGGGTCAGTACA
ACATTGGAAAGTCCATTAAATGCTTATCAGAAAAGTTTAGTATTGCAGAATCTGACCTCAAATACATGATTCGCCTGATTGACAGGTATGGGTTAGCCAT
TGTCCAAAAAGGTAAGAATAGTTATTATTCTCCAGAACTGAAGCAAGAGATAATAGATAAAGTTCTGATTGATGGTCAATCTCAAAAACAGACGTCCTTA
GACTATGCTTTACCAAATTCTAGTATGCTTTCAAGGTGGATAGCGCAATACAAGAAAAACGGCTATACTATTCTTGAGAAAAGAAGAGGGAGGCCACCAA
AGATGGGACGTCAACCAAAGAAGACTTTAGAACAAATGACAGAGTTGGAGCGACTCCAAAAAGAATTAGACTACCTTAGAGCGGAGAATGCTGTGCTAAA
AAAGCTGAGAGAATACCGGTTGAGGGACGAAGCAAAGCTCAAAGAGCAACAGAAATCATCCAAGAATTAATCGGTCAATTTTCTCTAGCAACTTTGCTTG
AAATCCTTGATTTATCGCGGTCAACCTATTATTATCAAGTCAAGCAACTAGCTCAAGAAGATAAGGACATGGACTTAAAGGAGCTCATTCAAGGCATCTA
TGATGAACATCATGGCAATTATGGCTATCGTCGCATTCATCTGGAACTAAGAAATCGTGGTTTTATCGTCAATCACAAAAAAGTACAACGTTTGATGACT
GTCATGGGCTTAAAAGCTCGTATCCGTCGTAAGCGCAAGTATTCTTCTTACAAAGGTGAGGTTGGCAAAAAGGCTGATAATCTGATTAAACGTCAGTTTG
AAGGTTCTAAGCCCTACGAGAAGTGCTATACCGATGTGACGGAATTTACCTTACCTGAGGGGAAACTCTATCTATCGCCTGTTCTTGACGGCTATAACAG
TGAGATTATTGATTTCACCCTGTCTCGATCGCCTGACTTGAAGCAAGTACAAACCATGCTTGAGAAGGCTTTTCCAGCGGATTCGTACAATGGAACGATT
CTCCACAGCGATCAAGGCTGGCAATATCAACATCAGTCTTATCATCACTTTTTGGAGACTAAAGGCATTCGTCCATCCATGTCTCGCAAGGGAAATAGTC
CAGATAATGGGATGATGGAGTCCTTCTTTGGTATTCTCAAATCTGAGATGTTTTACGGCCTTGAGACAACTTATCAATCCCTTAATGAGCTTGAACAAGC
TATTACAGATTACATTTTTTACTACAACAACAAACGCATTAAAGCAAAGCTAAAAGGACTTAGCCCTGTGCAATACAGAACTAAATCCTTTCACTAATAT
GTCGTGTCCAACTTTTGGGGGGTCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 405 bp | 134 aa | 166 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MIRLIDRYGLAIVQKGKNSYYSPELKQEIIDKVLIDGQSQKQTSLDYALPNSSMLSRWIAQYKKNGYTILEKRRGRPPKMGRQPKKTLEQMTELERLQKE
LDYLRAENAVLKKLREYRLRDEAKLKEQQKSSKN
LDYLRAENAVLKKLREYRLRDEAKLKEQQKSSKN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 480 | 1397 | + | No |
Description : Second part of the transposase
ORF sequence :
SGECCAKKAERIPVEGRSKAQRATEIIQELIGQFSLATLLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGFIVNHK
KVQRLMTVMGLKARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAFPA
DSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDYIFYYNNKRIKAKLKGLSPVQYR
TKSFH
KVQRLMTVMGLKARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLEKAFPA
DSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDYIFYYNNKRIKAKLKGLSPVQYR
TKSFH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1232 bp | 410 aa | 166 | 1397 | + | Yes |
Chemistry : DDE
ORF sequence :
MIRLIDRYGLAIVQKGKNSYYSPELKQEIIDKVLIDGQSQKQTSLDYALPNSSMLSRWIAQYKKNGYTILEKRRGRPPKMGRQPKKTLEQMTELERLQKE
LDYLRAENAVLKKAERIPVEGRSKAQRATEIIQELIGQFSLATLLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGF
IVNHKKVQRLMTVMGLKARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLE
KAFPADSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDYIFYYNNKRIKAKLKGLS
PVQYRTKSFH
LDYLRAENAVLKKAERIPVEGRSKAQRATEIIQELIGQFSLATLLEILDLSRSTYYYQVKQLAQEDKDMDLKELIQGIYDEHHGNYGYRRIHLELRNRGF
IVNHKKVQRLMTVMGLKARIRRKRKYSSYKGEVGKKADNLIKRQFEGSKPYEKCYTDVTEFTLPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQTMLE
KAFPADSYNGTILHSDQGWQYQHQSYHHFLETKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLNELEQAITDYIFYYNNKRIKAKLKGLS
PVQYRTKSFH
Blast result :
Comments
ISSeq3 is 86% aa similar to IS861.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Holden,M.T., Heather,Z., Paillot,R., Steward,K.F., Webb,K., Ainslie,F., Jourdan,T., Bason,N.C., Holroyd,N.E., Mungall,K., Quail,M.A., Sanders,M., Simmonds,M., Willey,D., Brooks,K., Aanensen,D.M., Spratt,B.G., Jolley,K.A., Maiden,M.C., Kehoe,M., Chanter,N., Bentley,S.D., Robinson,C., Maskell,D.J., Parkhill,J. and Waller,A.S. (2009) PLoS Pathog. 5 (3), E1000346
2] Submitted by ISfinder (2012).
2] Submitted by ISfinder (2012).