ISPa110
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas aeruginosa plasmid Escherichia coli CFS3273 |
DNA section
IS Length : 1598 bp
Ends
IR Length : 19/20
IRL : TGCGGACAAAAACTTGGACTACCAGGAGGTAGTTCATGTACTCATACGCA
IRR : TGCGGATAAAAACTTGGACTGGGTTTATGTGGTTAGGCCAAGTTTTTGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGGA | CCCCC | 0 |
DNA sequence
TGCGGACAAAAACTTGGACTACCAGGAGGTAGTTCATGTACTCATACGCAGACAGGATTCGTGCAGTTGAGCTTTACATCAAGCTTGGCTTACGTGTTAG
AGCCACGATTCGTCAGTTGGGATACCCAACCAAGAACGCATTGAAAGGCTGGTATCAGCACTACCTGAAGCATCAGGATTTGCCAGCAAGCCAAGTGCCA
AGAGCACCCAAATATTCGCTGCAGCAAAGACAGGTTGCGGTTGCTCATTACCTCGCCCACGATCGCTGTATTGCTGCCACGATGAGGGTTTTGGGTTACC
CGGGTCGAGGAACGTTGACTGCGTGGGTTCGGCAAGACTGCCCCGAGGCCAGCCAATCGATACTAGGCAGGTCTTGGCCGGTTACAAAGCCGGATGCTTT
GATGTATGAAGGCGTGGTGCAACTGTGTACCCGCCAATCAAGCGCACAGGAGATTGCGGACAAGTTAGGGGTATGCCGAGGAACCTTGTACAACTGGAAA
AATCAGTTGCTTGGCCCTTGCGCCCCCGCCTCGATGAAACACAGTCCAAAGCGATCGCCAGTGTTGGATGCAGCAGCGCTCAGGTGCCAAGTTGAATCAT
TGCGCCAGGATGTTCGGCGGCTGAAGATAGAGCGTGAACTTCTCAAGCAGGCCCATGAAATCTTAAAAAATGGGGCTGACATTGATCTGCATAGGCTGGC
AAACAAAGATAAGGCTGTGCTGGTTGAAGCGCTGCACGGGCAGTATGAATTGCCAGAACTGCTTTGCCATGTTGGCCTTGCTAGAAGCTCGTATTTTTAC
CATCGTGCCCGGCTGAAGCTGGCTGACAAATACTTGGATGTACGCCGAAGCATCACCGATATCTTCGACAGCAACTACCGTTGCTATGGATATCGCCGAG
TCCAGGCATCCTTGCTCAAGGAGTGCACGGGCATCTCAGAGAAGGTGGTTCGTCGGCTAATGAAGCAGGAGGGGTTGATCGTGGTCAAGCCCAAACGCCG
CAGATATAACTCATACCTTGGAGAGATAGGCGCTGCCCCGCAAAACCTCATCAATCGAGACTTCCATGCTGCTGCGCCGAATGAGAAGTGGCTTACCGAC
ATCACTGAATTCCAGATTCCTTCCGGCAAGGTTTACCTGTCGCCAGTGATTGATTGCTTTGACGGGCTGGTGGTGAGCTGGTCCATTGGGACTCATCCCA
ACGCCAATCTCGTCAACACAATGCTGGATGCAGCCATTGATGCTGTTCAAGGCAGTGAGAGCCGCCCTATGATTCATTCTGATCGTGGAGCTCACTACCG
CTGGCCGGGCTGGCTCTCGCGGGTGCACGATGCAAAGTTGGTTCGTTCAATGTCCCGCAAAGGGTGCTCACCAGACAACGCAGCATGCGAAGGCTTCTTT
GGAAGGCTGAAAATGGAGTTGTTCTATCCCGGCAACTGGCAATCAACGACGATTGAGCAGTTCATTGAAGCCGTCGATACTTACATCCGTTGGTACAACG
AAAAGCGCATCAAGGTATCTCTGGGGCGTTTAAGTCCTGTGGAATACAGGCAAAAACTTGGCCTAACCACATAAACCCAGTCCAAGTTTTTATCCGCA
AGCCACGATTCGTCAGTTGGGATACCCAACCAAGAACGCATTGAAAGGCTGGTATCAGCACTACCTGAAGCATCAGGATTTGCCAGCAAGCCAAGTGCCA
AGAGCACCCAAATATTCGCTGCAGCAAAGACAGGTTGCGGTTGCTCATTACCTCGCCCACGATCGCTGTATTGCTGCCACGATGAGGGTTTTGGGTTACC
CGGGTCGAGGAACGTTGACTGCGTGGGTTCGGCAAGACTGCCCCGAGGCCAGCCAATCGATACTAGGCAGGTCTTGGCCGGTTACAAAGCCGGATGCTTT
GATGTATGAAGGCGTGGTGCAACTGTGTACCCGCCAATCAAGCGCACAGGAGATTGCGGACAAGTTAGGGGTATGCCGAGGAACCTTGTACAACTGGAAA
AATCAGTTGCTTGGCCCTTGCGCCCCCGCCTCGATGAAACACAGTCCAAAGCGATCGCCAGTGTTGGATGCAGCAGCGCTCAGGTGCCAAGTTGAATCAT
TGCGCCAGGATGTTCGGCGGCTGAAGATAGAGCGTGAACTTCTCAAGCAGGCCCATGAAATCTTAAAAAATGGGGCTGACATTGATCTGCATAGGCTGGC
AAACAAAGATAAGGCTGTGCTGGTTGAAGCGCTGCACGGGCAGTATGAATTGCCAGAACTGCTTTGCCATGTTGGCCTTGCTAGAAGCTCGTATTTTTAC
CATCGTGCCCGGCTGAAGCTGGCTGACAAATACTTGGATGTACGCCGAAGCATCACCGATATCTTCGACAGCAACTACCGTTGCTATGGATATCGCCGAG
TCCAGGCATCCTTGCTCAAGGAGTGCACGGGCATCTCAGAGAAGGTGGTTCGTCGGCTAATGAAGCAGGAGGGGTTGATCGTGGTCAAGCCCAAACGCCG
CAGATATAACTCATACCTTGGAGAGATAGGCGCTGCCCCGCAAAACCTCATCAATCGAGACTTCCATGCTGCTGCGCCGAATGAGAAGTGGCTTACCGAC
ATCACTGAATTCCAGATTCCTTCCGGCAAGGTTTACCTGTCGCCAGTGATTGATTGCTTTGACGGGCTGGTGGTGAGCTGGTCCATTGGGACTCATCCCA
ACGCCAATCTCGTCAACACAATGCTGGATGCAGCCATTGATGCTGTTCAAGGCAGTGAGAGCCGCCCTATGATTCATTCTGATCGTGGAGCTCACTACCG
CTGGCCGGGCTGGCTCTCGCGGGTGCACGATGCAAAGTTGGTTCGTTCAATGTCCCGCAAAGGGTGCTCACCAGACAACGCAGCATGCGAAGGCTTCTTT
GGAAGGCTGAAAATGGAGTTGTTCTATCCCGGCAACTGGCAATCAACGACGATTGAGCAGTTCATTGAAGCCGTCGATACTTACATCCGTTGGTACAACG
AAAAGCGCATCAAGGTATCTCTGGGGCGTTTAAGTCCTGTGGAATACAGGCAAAAACTTGGCCTAACCACATAAACCCAGTCCAAGTTTTTATCCGCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1539 bp | 512 aa | 36 | 1574 | + | No |
Chemistry : DDE
ORF sequence :
MYSYADRIRAVELYIKLGLRVRATIRQLGYPTKNALKGWYQHYLKHQDLPASQVPRAPKYSLQQRQVAVAHYLAHDRCIAATMRVLGYPGRGTLTAWVRQ
DCPEASQSILGRSWPVTKPDALMYEGVVQLCTRQSSAQEIADKLGVCRGTLYNWKNQLLGPCAPASMKHSPKRSPVLDAAALRCQVESLRQDVRRLKIER
ELLKQAHEILKNGADIDLHRLANKDKAVLVEALHGQYELPELLCHVGLARSSYFYHRARLKLADKYLDVRRSITDIFDSNYRCYGYRRVQASLLKECTGI
SEKVVRRLMKQEGLIVVKPKRRRYNSYLGEIGAAPQNLINRDFHAAAPNEKWLTDITEFQIPSGKVYLSPVIDCFDGLVVSWSIGTHPNANLVNTMLDAA
IDAVQGSESRPMIHSDRGAHYRWPGWLSRVHDAKLVRSMSRKGCSPDNAACEGFFGRLKMELFYPGNWQSTTIEQFIEAVDTYIRWYNEKRIKVSLGRLS
PVEYRQKLGLTT*
DCPEASQSILGRSWPVTKPDALMYEGVVQLCTRQSSAQEIADKLGVCRGTLYNWKNQLLGPCAPASMKHSPKRSPVLDAAALRCQVESLRQDVRRLKIER
ELLKQAHEILKNGADIDLHRLANKDKAVLVEALHGQYELPELLCHVGLARSSYFYHRARLKLADKYLDVRRSITDIFDSNYRCYGYRRVQASLLKECTGI
SEKVVRRLMKQEGLIVVKPKRRRYNSYLGEIGAAPQNLINRDFHAAAPNEKWLTDITEFQIPSGKVYLSPVIDCFDGLVVSWSIGTHPNANLVNTMLDAA
IDAVQGSESRPMIHSDRGAHYRWPGWLSRVHDAKLVRSMSRKGCSPDNAACEGFFGRLKMELFYPGNWQSTTIEQFIEAVDTYIRWYNEKRIKVSLGRLS
PVEYRQKLGLTT*
Blast result :
Comments
ISPa110 is 80% aa similar to ISAtu3.
There is no -1 programmed frameshift.
There is no -1 programmed frameshift.
References
1] Urbanowicz P (2021) Direct submission.