ISPvu1

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP047346 ND Proteus vulgaris
Proteus vulgaris ZN3 plasmid pZN3-cfr-121kb
DNA section
IS Length : 2492 bp

Ends


IR Length : 24/26

IRL : GTAAGCGCCCTTAAATCAGCATCTTTTAACCCAGCCTATACAGCTTCACA
IRR : GTAAGCGCCCTTAAATCAGCACCCTTGCAAAACGATTACTCAGCCTAACC

Insertion site


Left flankDirect repeatRight flankDR Length
GTAAGCGCCCTTAAATCAGTTGCAACTGATTTAAGGGCGCTTAC8

DNA sequence

GTAAGCGCCCTTAAATCAGCATCTTTTAACCCAGCCTATACAGCTTCACACTAATACCTGTCTTTTTGAAAGGGGTATTGGTGATGACTTTACAGGAACG
CCAGCAGTTCTGGCAGCAACACATTGGTGCCTGGCAAGCATCTGATTCATCCGGTGCGGCTTTTTGCAAGCAGCATGATTTGAATTACGCGCAGTTTAAT
TATTGGCGTAAAAAGCTCCAGGTAAATGAGGTTGTTGAAAAACCTGCAGGCTTTGCTCAAGTGACACAGTTAGCTGCTTCGCACAACCGCCTTCATGATG
AGCTGGGCGTTCATTTACCCAGTGGCATAAGCTTTTCAGGGGTCAATGCAGGCAATTTGGATCTCGTCTTGGCACTGCTGAGGCAGCTGTAGTGAAAGTG
CGCTATTTGCGGCCAGCCAAACAGATGCCAGAAATTTATCTGTACCGTGCGCCGGTGGATTTTAGGAAACAAACCAACGGTTTAGCGTTGCTGGTGGAAC
AAGAGCTGGGCCACAATCCGTTCAGCGGTGCGCTGTATGCCTTCACCAATCGCCAGCGCAACAAAATCAAATGCGTGATGTGGGAAGACAACGGTTTCGT
CATGTATTACAAAGCGCTGGCCGAAGAGAAATTTAAGTGGCCCAAGCCTGCTGACGAGTTGCTGGCCTTAACAGGTGAGCAAATAAACTGGCTGTTAGAT
GGCTATGATATTAGTCTATTTAAGGGCCATAAAAAACTACAATACGACAGTCTTGGATAGCTTGATTTTATTGGGTTTTATCTGCTTTTACGGCTGTCTT
TCAGAGTCGTTTTTGCTATAATTTGGGTATGAAGAAACAGCCTAAACTCCCCATTAAAACATCTGATTTAAGCAGTCTAAGTGCTGCTGAATTACGTTTG
ATGGTGGCTCAATTTCAAGCGGTTGTAGAAGAACAAAATGCCAGTTTGTTAGAGCATAAAAGCGAGCTCAAAGCAGCCCATCGTCGTATCGAACTACTGG
AAGAAATGAACCGCCTGCTGAAAACGCAGAAGTTCAGTGCCAGCAGCGAAAAATCCAAGTTCCAGTTAAACCTCTTTGATGAAACTGAGCTTGAGGCTCA
GCTTGATGAGCTGTTCGATACCTTGCCTGAAGCGTCTGATGAAAACGCAGAGCTGACTCAAGCACGTAAAGCTCGCCAAACACGTCAGCGTGGTTTCTCT
GCAAATCTAGTACGTGAGCGCATCGAGCACACGCTAACTGAGTTAGAAAAAACCGGTGCCGACACAACCTTCTTCACCAAAGTAAAAGAAGAGCTACATT
ACATTCCGGCACAGCTGAAAGTGTTGGAGCATTGGCAAGAAAAAGCCGTTTTCACCTCCAAAGAAAGCATGCCAGAAGAACAGATGATTGCGGCGCAGCG
CCCAGTACATCCTTTTGGTAAGTGCTCGGTGACCACTTCGCTGATTGCGCACGTAATTGTAGATAAATATCTGTATGGCATGCCGTTGTATCGCCAAGAA
GGCAAGTTTAAAAACTTAGGCCACTTGATTCAGCGCAATAACATGGCGCAATGGTGCATGTGCTTTGCTGATGCAGCTAAACCTCTGCTACAGTTGATGC
GCGAGGTGCAAAACAGCGGCAGCTATCTGCAAGCTGATGAAACCCGTCTGCAAGTGCTGAAAGAAGACGGAGAAACAGCACAGTCAGATAAATGGATGTG
GGTGATCCGCGGCGGGCCGCCTAGCCAGCTTAGCGTACTCTTTGAATACGATCCTTCGCGTGCAGGCAGTGTGGCTGTGCGCCTGCTCGATGATTTTAAG
GGTGTGCTGCAAGCCGATGGTTTTTCTGGCTATAGCGCAGTCTGCAAAGCCAACTCGATTACGCGCATTGGCTGCTGGGATCATGCTCGGCGTAAGTTTG
TTGAAGCCGATAAAAGCGCTGATTCTAAAGCTAAAGCCAAGCAAGGCACAGTGAGTAAAGCTGATGTAGCGCTGGGCTATATCCGTAAACTCTATCGCGT
AGAAAGCAACATCGCGGACAAAACCGACGAAGAGCGTTTAAAGGCGCGCCAAGAAATCAGCGTGCCGGTACTGAATGAGTTTAAGCTGTGGCTTGAGAAA
AACGCTGCAAAAATCATGAAAGGCGGCGCTCTGAGAAAAGCCATCGACTACACCTTAAATCAATGGGAATACCTGATTGGCTATTGTGAGCGCGGCGACC
TGATGATCAGCAATATCATGGCCGAAAACGCCATTCGTCCCTTCGCTATTGGCCGTAAAAATTGGCTATTCGCCGACACACGCAAAGGCGCCGAAGCCAG
CGCCGCATGTTACTCATTAATCGAAACCGCCAAAGCCAACAACCTGGATCCGCAAGCCTACATTAAACATATTCTTGACCATGTTGCGACTGCTGACACC
CTCGAGAAACTCGAAGCGCTACTGCCCTGGAATGTGAAACTGGGTTAGGCTGAGTAATCGTTTTGCAAGGGTGCTGATTTAAGGGCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
309 bp102 aa84392+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTLQERQQFWQQHIGAWQASDSSGAAFCKQHDLNYAQFNYWRKKLQVNEVVEKPAGFAQVTQLAASHNRLHDELGVHLPSGISFSGVNAGNLDLVLALLR
QL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
369 bp122 aa392760+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MKVRYLRPAKQMPEIYLYRAPVDFRKQTNGLALLVEQELGHNPFSGALYAFTNRQRNKIKCVMWEDNGFVMYYKALAEEKFKWPKPADELLALTGEQINW
LLDGYDISLFKGHKKLQYDSLG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1620 bp539 aa8292448+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKQPKLPIKTSDLSSLSAAELRLMVAQFQAVVEEQNASLLEHKSELKAAHRRIELLEEMNRLLKTQKFSASSEKSKFQLNLFDETELEAQLDELFDTLP
EASDENAELTQARKARQTRQRGFSANLVRERIEHTLTELEKTGADTTFFTKVKEELHYIPAQLKVLEHWQEKAVFTSKESMPEEQMIAAQRPVHPFGKCS
VTTSLIAHVIVDKYLYGMPLYRQEGKFKNLGHLIQRNNMAQWCMCFADAAKPLLQLMREVQNSGSYLQADETRLQVLKEDGETAQSDKWMWVIRGGPPSQ
LSVLFEYDPSRAGSVAVRLLDDFKGVLQADGFSGYSAVCKANSITRIGCWDHARRKFVEADKSADSKAKAKQGTVSKADVALGYIRKLYRVESNIADKTD
EERLKARQEISVPVLNEFKLWLEKNAAKIMKGGALRKAIDYTLNQWEYLIGYCERGDLMISNIMAENAIRPFAIGRKNWLFADTRKGAEASAACYSLIET
AKANNLDPQAYIKHILDHVATADTLEKLEALLPWNVKLG

 

Blast result :
Comments
ISPvu1 is 73% aa (transposase) similar to ISAeme5.
References
1] Dongsheng Zhou (2020) Direct submission.