ISLmo11
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP007583.1 | ND | Listeria monocytogenes | Listeria monocytogenes Listeria monocytogenes XYSN |
DNA section
IS Length : 1513 bp
Ends
IR Length : 20/24
IRL : TGAGCTGACCCCCAAAAGTTAGACCTGAAAATCTAACTTTTGGAGGTTTT
IRR : TGTAGTGACCCCAAAAAGTTAGACTTTTTATAGAGTGGATTTTTCCACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATATCTTCTAACAAAG | ATA | TCTACGCTTTTTTATCT | 3 |
| TAATAATCAGAGAGCCTAGA | TTT | TCTAGGTTCTTTTTTTTATG | 3 |
| AAAGCCCTTCTTTCGGATAG | AAA | GAAGGGCTTTTTAAATTAAA | 3 |
| CTAATATAAAGAGACCTCGG | AAA | TTTTCCGAGGTCTTTTTTTT | 3 |
| AAAATAAAAAAAGTCTGGCA | TATT | TGCCAGACTTTTTTTTATAA | 4 |
| GATAAATAAAAAAGACCCGG | AAT | CCCGGGTCTTTTTTTAAATA | 3 |
| ACGCAAAAGAGCCTAGGGAG | ATT | GTTCCTATAGGCTCTTTTTC | 3 |
DNA sequence
TGAGCTGACCCCCAAAAGTTAGACCTGAAAATCTAACTTTTGGAGGTTTTTTTGTATGGCAAAATACCGTTTGGAATTTAAGCTTAACATTGTCCATGAC
TATTTATCTGGTCAAGGAGGTACAACATTTCTTGCAAAAAAGTATGGGTTTAAAAATACTTCTCAAGTAAGAAAATGGATAAATGCCTATAAAGAATTTG
GTGAAGAAGGACTACTTCGTAGTCGAAAAAGTAAGAATTACTCTGTTCAATTTAAGTTAGATGCGATAGAGTTGTACTTAACGACAGAGTTATCCTATCA
AGAACTAGCCAATCTTCTCAAAATGAACAATCCATCTTTGATTGCGAATTGGCTTAAAATGTATCGTACGCTTGGTATAGATGGACTCTCTAAACAGAAA
GGACGTCCACCTACAATGACTAAGAAGAAACGAAATGAAATCCAGCCATTACCAAGTGAACCATCTCAAGTAGAGAAACTAGAAAAAGAAAATAGAATGT
TAAAAATTGAGAATGCCTATTTAAAAGAATTGAGGAGGCTGCGTTTAGAGGACGAACAAAAAATGAACGAATCGCACGAATTATTCACAGCCTCCGAGGA
CACTTTCGATTAAAAGATATTCTAGAAACCCTAAGGTTTCCAAAGGCCACTTACATGTACTGGCAAAAACGCCTCGACAGAACCACTTCAACACAAAGGA
TGGAAGAAGAAATTCAAGCGATTCGTAAGGAACATCAACATTATGGCTATAGAAGAATGACACAGGAATTGAAACGACGTGGATATCAAGTAAATAAAAA
GAAAGTACAGCGATTAATGCAAAAGCTACAGTTACAAGTCAAAGCATTTACCAAGAAATCAAGGAAATATAATTCATATAAAGGAACTGTTGGAAAAGTA
GCTAAAAACTTGATTCATCGAAGATTCAACACTTCGGTCCCTCATCAAAAAATCACGACGGATACAACTGAATTTAAATACTATGAAACAGATGACGCAG
GCATTCTTCGACAGAAAAAACTATATTTAGATCCGTTTATGGATATGTATAACAGCGAAATTCTAAGCTACAGCCTTTCTACTCAGCCAAACGGTGTAAC
AGTTATTGCAGGTCTGGAAGAAGCAATTTCTCAAACAAATGATTGTTCCTATCGTCGCACCTTCCACTCTGACCAAGGCTGGGCGTATCAAATGAACACC
TATGTACAAACATTAAAGGATAACCGTATCTTTCAAAGTATGTCTCGGAAAGGAACCTGTTTAGATAATTCTCCTATGGAGAATTTCTTCAGTATTCTAA
AGCAAGAATTGTATTATGGAAAAGTTTATCAAAGTCAAAATGAGTTACGACAAGCCATTGAGAACTATATTTATTATTACAATCATTATAGAATCAAAGA
AAAGCTTAACTGGAAGAGTCCGGTAGAGTTTCGACGTTTCAATCAAAAAACTGCATAAAAATAGAGTGGAAAAATCCACTCTATAAAAAGTCTAACTTTT
TGGGGTCACTACA
TATTTATCTGGTCAAGGAGGTACAACATTTCTTGCAAAAAAGTATGGGTTTAAAAATACTTCTCAAGTAAGAAAATGGATAAATGCCTATAAAGAATTTG
GTGAAGAAGGACTACTTCGTAGTCGAAAAAGTAAGAATTACTCTGTTCAATTTAAGTTAGATGCGATAGAGTTGTACTTAACGACAGAGTTATCCTATCA
AGAACTAGCCAATCTTCTCAAAATGAACAATCCATCTTTGATTGCGAATTGGCTTAAAATGTATCGTACGCTTGGTATAGATGGACTCTCTAAACAGAAA
GGACGTCCACCTACAATGACTAAGAAGAAACGAAATGAAATCCAGCCATTACCAAGTGAACCATCTCAAGTAGAGAAACTAGAAAAAGAAAATAGAATGT
TAAAAATTGAGAATGCCTATTTAAAAGAATTGAGGAGGCTGCGTTTAGAGGACGAACAAAAAATGAACGAATCGCACGAATTATTCACAGCCTCCGAGGA
CACTTTCGATTAAAAGATATTCTAGAAACCCTAAGGTTTCCAAAGGCCACTTACATGTACTGGCAAAAACGCCTCGACAGAACCACTTCAACACAAAGGA
TGGAAGAAGAAATTCAAGCGATTCGTAAGGAACATCAACATTATGGCTATAGAAGAATGACACAGGAATTGAAACGACGTGGATATCAAGTAAATAAAAA
GAAAGTACAGCGATTAATGCAAAAGCTACAGTTACAAGTCAAAGCATTTACCAAGAAATCAAGGAAATATAATTCATATAAAGGAACTGTTGGAAAAGTA
GCTAAAAACTTGATTCATCGAAGATTCAACACTTCGGTCCCTCATCAAAAAATCACGACGGATACAACTGAATTTAAATACTATGAAACAGATGACGCAG
GCATTCTTCGACAGAAAAAACTATATTTAGATCCGTTTATGGATATGTATAACAGCGAAATTCTAAGCTACAGCCTTTCTACTCAGCCAAACGGTGTAAC
AGTTATTGCAGGTCTGGAAGAAGCAATTTCTCAAACAAATGATTGTTCCTATCGTCGCACCTTCCACTCTGACCAAGGCTGGGCGTATCAAATGAACACC
TATGTACAAACATTAAAGGATAACCGTATCTTTCAAAGTATGTCTCGGAAAGGAACCTGTTTAGATAATTCTCCTATGGAGAATTTCTTCAGTATTCTAA
AGCAAGAATTGTATTATGGAAAAGTTTATCAAAGTCAAAATGAGTTACGACAAGCCATTGAGAACTATATTTATTATTACAATCATTATAGAATCAAAGA
AAAGCTTAACTGGAAGAGTCCGGTAGAGTTTCGACGTTTCAATCAAAAAACTGCATAAAAATAGAGTGGAAAAATCCACTCTATAAAAAGTCTAACTTTT
TGGGGTCACTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 558 bp | 185 aa | 56 | 613 | + | No |
Description : First part of the transposase
ORF sequence :
MAKYRLEFKLNIVHDYLSGQGGTTFLAKKYGFKNTSQVRKWINAYKEFGEEGLLRSRKSKNYSVQFKLDAIELYLTTELSYQELANLLKMNNPSLIANWL
KMYRTLGIDGLSKQKGRPPTMTKKKRNEIQPLPSEPSQVEKLEKENRMLKIENAYLKELRRLRLEDEQKMNESHELFTASEDTFD
KMYRTLGIDGLSKQKGRPPTMTKKKRNEIQPLPSEPSQVEKLEKENRMLKIENAYLKELRRLRLEDEQKMNESHELFTASEDTFD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 315 aa | 511 | 1458 | + | No |
Description : Second part of the transposase
ORF sequence :
ECLFKRIEEAAFRGRTKNERIARIIHSLRGHFRLKDILETLRFPKATYMYWQKRLDRTTSTQRMEEEIQAIRKEHQHYGYRRMTQELKRRGYQVNKKKVQ
RLMQKLQLQVKAFTKKSRKYNSYKGTVGKVAKNLIHRRFNTSVPHQKITTDTTEFKYYETDDAGILRQKKLYLDPFMDMYNSEILSYSLSTQPNGVTVIA
GLEEAISQTNDCSYRRTFHSDQGWAYQMNTYVQTLKDNRIFQSMSRKGTCLDNSPMENFFSILKQELYYGKVYQSQNELRQAIENYIYYYNHYRIKEKLN
WKSPVEFRRFNQKTA
RLMQKLQLQVKAFTKKSRKYNSYKGTVGKVAKNLIHRRFNTSVPHQKITTDTTEFKYYETDDAGILRQKKLYLDPFMDMYNSEILSYSLSTQPNGVTVIA
GLEEAISQTNDCSYRRTFHSDQGWAYQMNTYVQTLKDNRIFQSMSRKGTCLDNSPMENFFSILKQELYYGKVYQSQNELRQAIENYIYYYNHYRIKEKLN
WKSPVEFRRFNQKTA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1403 bp | 467 aa | 56 | 1458 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKYRLEFKLNIVHDYLSGQGGTTFLAKKYGFKNTSQVRKWINAYKEFGEEGLLRSRKSKNYSVQFKLDAIELYLTTELSYQELANLLKMNNPSLIANWL
KMYRTLGIDGLSKQKGRPPTMTKKKRNEIQPLPSEPSQVEKLEKENRMLKIENAYLKELRRLRLEDEQKMNESHELFTASEDNFRLKDILETLRFPKATY
MYWQKRLDRTTSTQRMEEEIQAIRKEHQHYGYRRMTQELKRRGYQVNKKKVQRLMQKLQLQVKAFTKKSRKYNSYKGTVGKVAKNLIHRRFNTSVPHQKI
TTDTTEFKYYETDDAGILRQKKLYLDPFMDMYNSEILSYSLSTQPNGVTVIAGLEEAISQTNDCSYRRTFHSDQGWAYQMNTYVQTLKDNRIFQSMSRKG
TCLDNSPMENFFSILKQELYYGKVYQSQNELRQAIENYIYYYNHYRIKEKLNWKSPVEFRRFNQKTA
KMYRTLGIDGLSKQKGRPPTMTKKKRNEIQPLPSEPSQVEKLEKENRMLKIENAYLKELRRLRLEDEQKMNESHELFTASEDNFRLKDILETLRFPKATY
MYWQKRLDRTTSTQRMEEEIQAIRKEHQHYGYRRMTQELKRRGYQVNKKKVQRLMQKLQLQVKAFTKKSRKYNSYKGTVGKVAKNLIHRRFNTSVPHQKI
TTDTTEFKYYETDDAGILRQKKLYLDPFMDMYNSEILSYSLSTQPNGVTVIAGLEEAISQTNDCSYRRTFHSDQGWAYQMNTYVQTLKDNRIFQSMSRKG
TCLDNSPMENFFSILKQELYYGKVYQSQNELRQAIENYIYYYNHYRIKEKLNWKSPVEFRRFNQKTA
Blast result :
Comments
ISLmo11 is 79% aa (transposase) similar to ISEfa10.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISLmo11 was identified by in silico sequence analysis of Listeria monocytogenes strain XYSN, where it is present in multiple copies.
Ends based on empty insertion sites in other strains.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISLmo11 was identified by in silico sequence analysis of Listeria monocytogenes strain XYSN, where it is present in multiple copies.
Ends based on empty insertion sites in other strains.
References
1] Chmielowska, C. and Bartosik, D. (2020) Direct submission.
2] Yin Y, Yao H, Doijad S, et al. A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates. Nat Commun. 2019;10(1):4283. doi:10.1038/s41467-019-12072-1
2] Yin Y, Yao H, Doijad S, et al. A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates. Nat Commun. 2019;10(1):4283. doi:10.1038/s41467-019-12072-1