ISPa124
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP041774.1 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa 60503 |
DNA section
IS Length : 867 bp
Ends
IR Length : 18/19
IRL : GAGCCGCTAACAAAACCGTTTTGAACGGCCGAGCATTGATGGCAAAATCG
IRR : GAGCGGCTAACAAAACCGTGGAAGGTGGCGAAGGCAGATGATCGAACAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATGGTGAACTAA | TTAATCGAGAAAC | 0 | |
| AGCCTGCGGC | TAA | GCCTCAGAAC | 3 |
| GCAATGAAAC | TAA | GCATGTTGGC | 3 |
DNA sequence
GAGCCGCTAACAAAACCGTTTTGAACGGCCGAGCATTGATGGCAAAATCGTCGGTATGGCCCGTCGCAAAGAGATTCCCGCTGCGCTGTGGAAGCGCATC
GAACCGTTGATCCCGCCAGTGAAGCCCTCGCCCAAGGGAGGGCGACCGCGCGTCAGCGACCAGCAAGCCCTCAATGGCATCATCTACGTCCTGCGCACCG
GTATTCCCTGGGAAGAGCTGCCGCTGGAACTGGGCTACGGCAGCGGCATGACCTGCTGGCGGCGTCTGCGTGACTGGCAGGCGGCTGGGGTGTGGCATCG
TTTGCATCAGGTCCTGCTGGCCGAACTGCGCCGGGCCGACAAACTGGACCTGAGCCGGACAAGCCTGGATGCGGCCAGCGTGGCCTCCCCCCGGGGGGCG
CTTATACCGGGCCGAACCCGACCGATCGCGGCAAGCTCGGCAGCAAACGGCACCTGATCACCGATCGCAACGGCATTCCCCTGGCGTTCTGCGTCACCGG
AGCCAACCGACATGACTCGGTGGTCTTCGAGGAGTTGGTCGAGGCCTTGCCTGCGATCGCCGGCAAGCCGGGACGCCCACGACGCTGGCCGGACAAACTG
CATGCCGACAAGGCCTACGACATCGAGCGCTGCCGCGCGCATCTCAAACAACGAAGGATCACCGCTCGTATCGCTCGCAAAGGAATCGAGCGCAACGACC
GGCTCGGGCGACATCGCTGGGTGGTGGAGCGCACTCATGCCTGGTTCGCGGGCATGGGCAAGCTGCGCATTCGATTCGAGCGCCGCATCGATATCCATCT
GGCCTGGCTGTCACTCGCCTGTTCGATCATCTGCCTTCGCCACCTTCCACGGTTTTGTTAGCCGCTC
GAACCGTTGATCCCGCCAGTGAAGCCCTCGCCCAAGGGAGGGCGACCGCGCGTCAGCGACCAGCAAGCCCTCAATGGCATCATCTACGTCCTGCGCACCG
GTATTCCCTGGGAAGAGCTGCCGCTGGAACTGGGCTACGGCAGCGGCATGACCTGCTGGCGGCGTCTGCGTGACTGGCAGGCGGCTGGGGTGTGGCATCG
TTTGCATCAGGTCCTGCTGGCCGAACTGCGCCGGGCCGACAAACTGGACCTGAGCCGGACAAGCCTGGATGCGGCCAGCGTGGCCTCCCCCCGGGGGGCG
CTTATACCGGGCCGAACCCGACCGATCGCGGCAAGCTCGGCAGCAAACGGCACCTGATCACCGATCGCAACGGCATTCCCCTGGCGTTCTGCGTCACCGG
AGCCAACCGACATGACTCGGTGGTCTTCGAGGAGTTGGTCGAGGCCTTGCCTGCGATCGCCGGCAAGCCGGGACGCCCACGACGCTGGCCGGACAAACTG
CATGCCGACAAGGCCTACGACATCGAGCGCTGCCGCGCGCATCTCAAACAACGAAGGATCACCGCTCGTATCGCTCGCAAAGGAATCGAGCGCAACGACC
GGCTCGGGCGACATCGCTGGGTGGTGGAGCGCACTCATGCCTGGTTCGCGGGCATGGGCAAGCTGCGCATTCGATTCGAGCGCCGCATCGATATCCATCT
GGCCTGGCTGTCACTCGCCTGTTCGATCATCTGCCTTCGCCACCTTCCACGGTTTTGTTAGCCGCTC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 382 bp | 133 aa | 56 | 437 | + | No |
Description : First part of the transposase
ORF sequence :
MARRKEIPAALWKRIEPLIPPVKPSPKGGRPRVSDQQALNGIIYVLRTGIPWEELPLELGYGSGMTCWRRLRDWQAAGVWHRLHQVLLAELRRADKLDLS
RTSLDAASVASPRGALIPGRTRPIAASSAANGT
RTSLDAASVASPRGALIPGRTRPIAASSAANGT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 588 bp | 195 aa | 274 | 861 | + | No |
Description : Second part of the transposase
ORF sequence :
LAGGWGVASFASGPAGRTAPGRQTGPEPDKPGCGQRGLPPGGAYTGPNPTDRGKLGSKRHLITDRNGIPLAFCVTGANRHDSVVFEELVEALPAIAGKPG
RPRRWPDKLHADKAYDIERCRAHLKQRRITARIARKGIERNDRLGRHRWVVERTHAWFAGMGKLRIRFERRIDIHLAWLSLACSIICLRHLPRFC
RPRRWPDKLHADKAYDIERCRAHLKQRRITARIARKGIERNDRLGRHRWVVERTHAWFAGMGKLRIRFERRIDIHLAWLSLACSIICLRHLPRFC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 806 bp | 268 aa | 56 | 861 | + | Yes |
Chemistry : DDE
ORF sequence :
MARRKEIPAALWKRIEPLIPPVKPSPKGGRPRVSDQQALNGIIYVLRTGIPWEELPLELGYGSGMTCWRRLRDWQAAGVWHRLHQVLLAELRRADKLDLS
RTSLDAASVAFPPGGAYTGPNPTDRGKLGSKRHLITDRNGIPLAFCVTGANRHDSVVFEELVEALPAIAGKPGRPRRWPDKLHADKAYDIERCRAHLKQR
RITARIARKGIERNDRLGRHRWVVERTHAWFAGMGKLRIRFERRIDIHLAWLSLACSIICLRHLPRFC
RTSLDAASVAFPPGGAYTGPNPTDRGKLGSKRHLITDRNGIPLAFCVTGANRHDSVVFEELVEALPAIAGKPGRPRRWPDKLHADKAYDIERCRAHLKQR
RITARIARKGIERNDRLGRHRWVVERTHAWFAGMGKLRIRFERRIDIHLAWLSLACSIICLRHLPRFC
Blast result :
Comments
ISPa124 is 82% (transposase) aa similar to IS1421.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.