ISPa122

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
DNA section
IS Length : 2358 bp

Ends


IR Length : 17/24

IRL : GTAAGCGCCCGGTGAACACACCTTCTGAACTCCAGCATTAAGGGCGATGG
IRR : GTAAGCGTATGGGGAATACGTCTTGCGCAGGTCAGCCGGGCATCCATTGA

Insertion site


Left flankDirect repeatRight flankDR Length
CCGG0

DNA sequence

GTAAGCGCCCGGTGAACACACCTTCTGAACTCCAGCATTAAGGGCGATGGTGTCCGCCTATTTTTTAGCGGACGCGATCGCCCTTATCGCCAGGATTTCC
ATGCGCCAACGAAGCTCTTATCCAAAAACCTTCAAGGCCCAGGTTGTTCAGGAGTGCCTGCAACCGGGGGCCACTGTTTCCGGCGTCGCCATCAGCCATG
GCATCAACGCCAACGTGATCCGCAAGTGGCTGCCGATTTACCGCGACCGACACCCTACCGGCCTGCCGACATTCGTGCCGGTACAAGCGACTGCCAGGCG
GCCGGCTGAAGAAGTGGCGGTGATCACATTGCCGCTGGGTGACACATCCATCACGGTCAAATGGCCCGTCTCAGATCCGGACGGCTGCGCGCGCTTCATC
CGAAGCCTCTCGCAATGATCCGCATCGACGCGATCTGGCTCGCCACCCAGCCCATGGACATGCGCGCCGGCACTGACACCGCGCTGGCCCGCGTGGTTGC
GGTATTCGGTGCGGCGAAGCCGCACTGCGCTTATCTGTTCGCCAACCGCCGCGCCAATCGGATGAAAGTGCTCGTGCACGATGGTGTAGGTATTTGGCTT
GCTGCACGGCGGTTGAACCAAGGGAAATTCCACTGGCCCGGCATTCATCACGGCCTGGAAGTCGAACTCGACGCCGAACAGCTTCACGCCCTGGTGCTCG
GTTTGCCATGGCGACGGGTTGGTTCCGGCGGCACAATTACATTGCTTTGAACTTCGCTCTTTAGCTGTGGCAGATGGGCTGCTTTGGTAAAATCCACGGT
ATGAGTTCCTCGCCCAATCTCGACCAAATGACGCCCGATCAGCTGCGCGCACTCGCTGCACAGTTGCTGTCGAAGGTCGACACCATGGGCAGAAAGATCC
ATCGCGATGAGACGATCATCGAGCAGCTCACTCACGAGATCGCCATCCTCAAACGGCACAAATACGCCAAGCGCAGCGAGCAAATCAGCCCTGCGCAAGG
CAGCTTGCTGGATGACCTGCTCAATACCGACCTTGAGGCTATCGAAGCAGAACTGAACGCGCTGCGTCCCGCCCCGACGTCGGACCAAACCCGCCAAAAG
CCCAAGCGCGCACCTCTGCCGCCGCAGTTCCCACGTACCGTCATTCACCACGAGCCGCAGAGCACCGAATGCGTCTGCGGCTGCCAGCTTCAGCGCATCG
GTGAAGACGTCAGCGAGAAGCTGGATTACACCCCGGGTGTGTTTACCGTCGAGCAACATGTGCGTGGCAAGTGGGCCTGCCATCAGTGTGAAACCCTGAT
CCAGGCGCCCGTGCCGGCCCAGGTGATCGACAAGGGTATCCCTACCGCCGGCCTGCTGGCTCACGTGATGGTGGCCAAGTTCGCCGATCACCTGCCACTG
TACCGGCAGGAAAAAATCTTTGGCCGGGCTGGTCTAGCGATTCCCCGCTCGACGCTGGCTCAGTGGGCGGGCCAGACCGGCGTACAGCTCCAGCCACTGG
TGGATGCGTTGCGCAAAACGGTGCTGGCTCAGAGCGTCGTACACGCGGATGAAACACCGGTTCAAATGCTGGCACCGGGCGAGAAGAAAACACACCGGGC
TTACGTCTGGGCCTACTGCACCACACCATTCTCGGCACTGAAGGCCGTGGTCTACGACTTCAGCCCCAGCCGTGCAGGCGAACATGCGCGTAACTTCCTG
GGCACCTGGAACGGCAAGCTGGTCTGCGATGACTTCTCGGGCTACAAGGCTGGCTTCGAGCAGGGCATCACCGAAATCGGCTGCATGGCTCATGCACGGC
GCAAGTTCTTCGACCTGCATGTGGCGAACAAAAGCCAACTGGCCGAACAGGCGCTGCACTCTATCGCCGGGCTGTACGAAGTCGAGCGGCAGGCCAAGGA
AATGAGCGATGAAGGTCGATGGCGATTACGCCAGGAGCTAGCGGTGCCTATCGCAGGCAAGTTGCATGAATGGATGTTTGCTCAGCGCGACCTTGTGCCC
GACGGGTCAGCTACGGCCAAGGCCTTGGATTACAGCTTGAAACGCTGGGTAGCGCTGACGCGCTACCTGGACGATGGTGCTGTGCCCATCGACAACAATG
CGGTCGAGAACCAGATCAGGCCGTGGGCGCTCGGGCGCGCGAACTGGCTCTTCGCTGGATCACTGCGCAGCGGCAAGCGGGCGGCGGCGATCATGAGCCT
GATCCAGTCGGCGCGGATGAATGGGCATGATCCGTACGCGTACCTCAAGGATGTACTGACGCGGTTGCCGACGCAGAAGGCCAGTGAGATCGAGCACCTG
TTGCCGCATCAATGGATGCCCGGCTGACCTGCGCAAGACGTATTCCCCATACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
650 bp105 aa101750+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MRQRSSYPKTFKAQVVQECLQPGATVSGVAISHGINANVIRKWLPIYRDRHPTGLPTFVPVQATARRPAEEVAVITLPLGDTSITVKWPVSDPDGCARFI
RSLSQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
336 bp129 aa415750+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MARLRSGRLRALHPKPLAMIRIDAIWLATQPMDMRAGTDTALARVVAVFGAAKPHCAYLFANRRANRMKVLVHDGVGIWLAARRLNQGKFHWPGIHHGLE
VELDAEQLHALVLGLPWRRVGSGGTITLL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1527 bp508 aa8012327+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSSSPNLDQMTPDQLRALAAQLLSKVDTMGRKIHRDETIIEQLTHEIAILKRHKYAKRSEQISPAQGSLLDDLLNTDLEAIEAELNALRPAPTSDQTRQK
PKRAPLPPQFPRTVIHHEPQSTECVCGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACHQCETLIQAPVPAQVIDKGIPTAGLLAHVMVAKFADHLPL
YRQEKIFGRAGLAIPRSTLAQWAGQTGVQLQPLVDALRKTVLAQSVVHADETPVQMLAPGEKKTHRAYVWAYCTTPFSALKAVVYDFSPSRAGEHARNFL
GTWNGKLVCDDFSGYKAGFEQGITEIGCMAHARRKFFDLHVANKSQLAEQALHSIAGLYEVERQAKEMSDEGRWRLRQELAVPIAGKLHEWMFAQRDLVP
DGSATAKALDYSLKRWVALTRYLDDGAVPIDNNAVENQIRPWALGRANWLFAGSLRSGKRAAAIMSLIQSARMNGHDPYAYLKDVLTRLPTQKASEIEHL
LPHQWMPG

 

Blast result :
Comments
ISPa122 is 94% aa (transposase) similar to ISPpu30.
References
1] Dongsheng Zhou (2020) Direct submission.