ISHgi7

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Haloferax gibbonsii
Haloferax gibbonsii plasmid pHGLR1
DNA section
IS Length : 1886 bp

Ends


Left end : TTACGAAAAATTCGAGGCGAAAGCCTCGCCCTTCAGGGCGGGGATGAAGTCGACCAATAGTATTCAACCGCCGACGACAGCACAGACGGGGTTCCAACGC II struct. : No
Right end : TCCCAAAGAAGTGCGCACAAACCCGCAAGTTGCCTCCGTGGGTCGGTAGCCGAACCCCCAATGGAGGAATCCTCGCGCTTTAGCGCGGGGAGGATGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TATGGACCGGTCTTACGCCTCGGTTAGGTCAA
GTTTCGACCCGATTACGAGTCGGTCGGTTCAA

DNA sequence

GAAAAATTCGAGGCGAAAGCCTCGCCCTTCAGGGCGGGGATGAAGTCGACCAATAGTATTCAACCGCCGACGACAGCACAGACGGGGTTCCAACGCAATC
TTTAACCCGCCCGGTTGTGATAGTATGCATAGATGGTAAAGAGTACCCGTCACGCGAAATACGAACTCTACTACCACATAGTGTTCGTGCCGAAATATCG
GCGTTCGAACCTGACGGGGAAGACAAAGGAACGTCTCGAAGCCATCTTCGCGGAAATCTGTGAGGACAAAGACCTCGAACTGGCCGAGTCCGAGGTCATG
CCCGACCACGTACACCTGTTCATCGGGAGTCCACCCAAGAACGCACCGTCACTCATCGTCAACTGGGTCAAGGGCATCTCGGCGCGGAAGTATAACCAGC
GGTACGACGACCGCGTGAAGTGGACTCGTTCCTACTACGTCGGAACAGCGGGAAGCGCCTCGAAGGGCGCTGTCGAACAGTATATCGCTGAACAGGAAGG
TAGCGACGAATGAAGCGCGTCAACACCTTCGAGGTACTTCCACAGACCGAGAACGACAAAGAGTGCCTTCTACGGCTCCTCGACGCCTCCGCTTCCCTGT
GGAACGAACTGACCTACGAACGTCGCCAGAACTACTTCGGTGACGGCGACGTGTGGGACACCTCCGAGTACCGTGGACAGTACAACGGCGTCGTCGGAAG
CGCGACCGTCCAACAGGTCACGCGCAAGAACAGCGAAGCGTGGCGGTCGTTCTTTGCCAAGGAGAAAGGCGAGTACGCCAACCCACCATCGTACTGGGGC
AACGAAGAGGACGGACGCGAACTCCGCACCTACATCCGATGCAACCAGTACACGATTGAGTGGGGGAAGCGTAGTCGTCTCGAAATCCCTGTCGGGCAAG
AACTGAAAGACGAATACGGACTCGGCTACCACGAACGACTCCGCCTCGAAGTCCGAGGCAACCCGAAATGGGACGGCAAACAGGGTCGTCTGGAACTTGA
GTACGACGAGGTTAGCGACACGTTCAGGGCTTTCCAACCAGTCACCGTACCTGATTCTCGACTGGGTTCACCACTGGCTTCTCACGAAGCCGCCCTCGAC
GTTGGCGCGAACAATCTCGTCGCCTGTTCCACGACCACTGGGAACCAGTACCTCTACGACGGCCGGGAGTTGTTCAGACGGTTCCGCGAGACGACAGACG
AAATCGCCCGCCTACAGTCGAAATTGCCCGAGAGACGGAGTCTCTCGGAATCACGGAAGACGGAGTCTTCCGGACGACTCCGAGAGGGGCGCTACTCCTC
GAATCGGATTCGACGGCTGTACCGGCAGCGAACGAAACGCCGTGACCACGCACAGAACGCGCTGGTGCGCGACCTCGTTGAACGGCTGTACGACGAGGGC
GTGGCGACGGTGTACGTGGGCGATTTGACCGACGTGCTGGAAACGCATTGGTCGGTCAGGGTGAACGAGAAGACGCACAACTTCTGGGCGTTCAAGAAGT
TCATCCACCGTCTCGCGTGTGTCTGTGAGGAGTACGGCATCTCTCTCGAAGCCGAGTCGGAAGCGTGGACGAGTCAGACGTGTCCCGAGTGTGGCGACCA
CGAGGAGACGGTTCGCCACGGGGATACGCTGACGTGTCCGTGTGGTTTCGAGGGACACGCCGACCTCACGGCGTCAGAGACGTTCCTTCGGGAAAACAGC
GATTGCGAAATCAGGCCGATGGCACGGCCCGTGCGATTTGAGTGGGACGACCACGACTGGTCGGGGAAACCACACCCTCACGAAAGTCCCAAAGAAGTGC
GCACAAACCCGCAAGTTGCCTCCGTGGGTCGGTAGCCGAACCCCCAATGGAGGAATCCTCGCGCTTTAGCGCGGGGAGGATGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
381 bp126 aa133513+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MVKSTRHAKYELYYHIVFVPKYRRSNLTGKTKERLEAIFAEICEDKDLELAESEVMPDHVHLFIGSPPKNAPSLIVNWVKGISARKYNQRYDDRVKWTRS
YYVGTAGSASKGAVEQYIAEQEGSDE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1326 bp441 aa5101835+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRVNTFEVLPQTENDKECLLRLLDASASLWNELTYERRQNYFGDGDVWDTSEYRGQYNGVVGSATVQQVTRKNSEAWRSFFAKEKGEYANPPSYWGNEE
DGRELRTYIRCNQYTIEWGKRSRLEIPVGQELKDEYGLGYHERLRLEVRGNPKWDGKQGRLELEYDEVSDTFRAFQPVTVPDSRLGSPLASHEAALDVGA
NNLVACSTTTGNQYLYDGRELFRRFRETTDEIARLQSKLPERRSLSESRKTESSGRLREGRYSSNRIRRLYRQRTKRRDHAQNALVRDLVERLYDEGVAT
VYVGDLTDVLETHWSVRVNEKTHNFWAFKKFIHRLACVCEEYGISLEAESEAWTSQTCPECGDHEETVRHGDTLTCPCGFEGHADLTASETFLRENSDCE
IRPMARPVRFEWDDHDWSGKPHPHESPKEVRTNPQVASVGR

 

Blast result :
Comments
ISHgi7 is 98% (transposase) aa similar to ISHla16.
References
1] Friedhelm Pfeiffer (2020) Direct submission.
2] Tittes, C., Schwarzer, S., Pfeiffer, F., Dyall-Smith, M., Oksanen, H., Quax, T. (in preparation)