ISH27-1
- Family ISH3
- Group
Isoform Synonym(s) ISH61
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X54432 | Y | Halobacterium salinarum | Halobacterium tunesiensis Halobacterium sp. SB3 Halobacterium salinarum PHH1 plasmid pHH1 |
DNA section
IS Length : 1398 bp
Ends
IR Length : 16
IRL : CAGTACATCACAAAGCCGGTTAGTTGAGACGTCTGCTTGCTGAAGGTGTG
IRR : CAGTACCTCACAAAGCATTCTCGGCTAGCTGTTTCTGAAGCCTGAGTTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTCTAGTCC | AGCTT | TCCTCACAGC | 5 |
| CATCCAGTGC | AAAAT | AAGCCTCGAC | 5 |
| GTTTGTCGGC | AGTCT | GGCGTATGTC | 5 |
| GCTGGAGATA | ATTCT | TGGTTAAGTT | 5 |
| GTCTTGATTG | AGACT | GTCCGGCTAC | 5 |
| GTCAACGGGC | AAGTT | NNNNNNNNNN | 5 |
| NNAACGGGCA | AGGTT | AGTCGTTNNN | 5 |
| NNNNNNNNNN | ATCCT | NNNNNNNNNN | 5 |
DNA sequence
CAGTACATCACAAAGCCGGTTAGTTGAGACGTCTGCTTGCTGAAGGTGTGTCTACAACCCAGCAAGCAGACGGTGAAATCCACGAGGACCAGCTTCTTAA
CTTCCTCGTCAACGCTCTTGACGAGGAAGTCGCCCTCACACTCGCTGAAAACGCCGAGATCGATGCTGAAGACATCTACGAGGTCCTCGTCGGCGCGTGC
GTCCACGGGACCTCGGTCTCAACGCTCTGTGAGAAAAGCGAAGATGCCCCCCACGAAAATTCGGTTCTCTACCATCTCCGCACCAAATTCGACCTTGAGA
CGCTTGAGCAAGTCGGAAACGCGCTCCTTCAGAAGGACGTTCTCGACGTCCTTCCCCAGCAGGTGGAGGTCGTTTCGGACCTCCACCTGCGGCCCTACTA
CGGCGATGAAGACGGGACAGACGGCCTGTATCACTCACAAGCGAAGCGTGGAACAACTGCGTTCCACGCCTACGCCACACTCTACGCGCGTGTGAAGAAC
AAACGCTACACGCTGGCGGTGCGCCGTCTCGAAGACGGCGACACCGCCAGCAGTGTCCTCGCAGAGTTTCTCGGTATCCTTCGACGCCTTGACCTCGACG
TCAAGGCCGTCTTTCTTGACCGCGAATTCTACGACAGCAAGTGTTTGACGCTGCTTCAGGCACACAACCACGCCTACGTCATGCCGATCGTCCGCTGGGG
ACAGACGATCAAGCAAGAACTCTCGGAAGGCTGGAGTCGCGTCATTCAACACGACATGACGGCGAAACTCGACGGTCACAGCTGGACCGTCGAGTTTCTC
GTCTACATCGACTGTACGTACCAAAACGGACGGTGCGACGAACATGGGGTGGCGCGTCACGGCTACGCCGCTGACGCGCCGTTCATCGAGAGTCCACGCG
ACGCTCGATACCACTACGCGAAACGCTTCGGTATCGAAGGCAGCTATCGACTCTCTGAACAAAGTATTGCGACGACCTCAACACAAAATCCGGTCGTACG
GCTGCTGTACGTCGTGGTGAGCTTGTTGTTACAAAACGTCTGGCGGTATTTACATTGGGAGTACGTGCGGACGCCCCGCCGTGGGGGGCGTCGCCTCTGG
AAGTGGTCGTTCAAGGAGTTCATCAACATGATTCAACGGGCAGCGTGGACGGCCCTCGCGACGCGTCGGGCCGTCCCCGCGAACCGACCGCCAGACGACC
GGTTTACCCGCTAATCCCCGACCAGGGTAGCCTTCGCCAGGAGTGGCGACGCTGTCGCGTCGGCGGCAGCCGCCGCCGACAGCGACAGCTCTCCGTCGGT
TCGTCGGAGATCTTCTCGTCAAGACCACCAGCACAACAGCTCCGCCGTGGAACTCAGGCTTCAGAAACAGCTAGCCGAGAATGCTTTGTGAGGTACTG
CTTCCTCGTCAACGCTCTTGACGAGGAAGTCGCCCTCACACTCGCTGAAAACGCCGAGATCGATGCTGAAGACATCTACGAGGTCCTCGTCGGCGCGTGC
GTCCACGGGACCTCGGTCTCAACGCTCTGTGAGAAAAGCGAAGATGCCCCCCACGAAAATTCGGTTCTCTACCATCTCCGCACCAAATTCGACCTTGAGA
CGCTTGAGCAAGTCGGAAACGCGCTCCTTCAGAAGGACGTTCTCGACGTCCTTCCCCAGCAGGTGGAGGTCGTTTCGGACCTCCACCTGCGGCCCTACTA
CGGCGATGAAGACGGGACAGACGGCCTGTATCACTCACAAGCGAAGCGTGGAACAACTGCGTTCCACGCCTACGCCACACTCTACGCGCGTGTGAAGAAC
AAACGCTACACGCTGGCGGTGCGCCGTCTCGAAGACGGCGACACCGCCAGCAGTGTCCTCGCAGAGTTTCTCGGTATCCTTCGACGCCTTGACCTCGACG
TCAAGGCCGTCTTTCTTGACCGCGAATTCTACGACAGCAAGTGTTTGACGCTGCTTCAGGCACACAACCACGCCTACGTCATGCCGATCGTCCGCTGGGG
ACAGACGATCAAGCAAGAACTCTCGGAAGGCTGGAGTCGCGTCATTCAACACGACATGACGGCGAAACTCGACGGTCACAGCTGGACCGTCGAGTTTCTC
GTCTACATCGACTGTACGTACCAAAACGGACGGTGCGACGAACATGGGGTGGCGCGTCACGGCTACGCCGCTGACGCGCCGTTCATCGAGAGTCCACGCG
ACGCTCGATACCACTACGCGAAACGCTTCGGTATCGAAGGCAGCTATCGACTCTCTGAACAAAGTATTGCGACGACCTCAACACAAAATCCGGTCGTACG
GCTGCTGTACGTCGTGGTGAGCTTGTTGTTACAAAACGTCTGGCGGTATTTACATTGGGAGTACGTGCGGACGCCCCGCCGTGGGGGGCGTCGCCTCTGG
AAGTGGTCGTTCAAGGAGTTCATCAACATGATTCAACGGGCAGCGTGGACGGCCCTCGCGACGCGTCGGGCCGTCCCCGCGAACCGACCGCCAGACGACC
GGTTTACCCGCTAATCCCCGACCAGGGTAGCCTTCGCCAGGAGTGGCGACGCTGTCGCGTCGGCGGCAGCCGCCGCCGACAGCGACAGCTCTCCGTCGGT
TCGTCGGAGATCTTCTCGTCAAGACCACCAGCACAACAGCTCCGCCGTGGAACTCAGGCTTCAGAAACAGCTAGCCGAGAATGCTTTGTGAGGTACTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 388 aa | 48 | 1214 | + | No |
Chemistry : DDE
ORF sequence :
MSTTQQADGEIHEDQLLNFLVNALDEEVALTLAENAEIDAEDIYEVLVGACVHGTSVSTLCEKSEDAPHENSVLYHLRTKFDLETLEQVGNALLQKDVLD
VLPQQVEVVSDLHLRPYYGDEDGTDGLYHSQAKRGTTAFHAYATLYARVKNKRYTLAVRRLEDGDTASSVLAEFLGILRRLDLDVKAVFLDREFYDSKCL
TLLQAHNHAYVMPIVRWGQTIKQELSEGWSRVIQHDMTAKLDGHSWTVEFLVYIDCTYQNGRCDEHGVARHGYAADAPFIESPRDARYHYAKRFGIEGSY
RLSEQSIATTSTQNPVVRLLYVVVSLLLQNVWRYLHWEYVRTPRRGGRRLWKWSFKEFINMIQRAAWTALATRRAVPANRPPDDRFTR
VLPQQVEVVSDLHLRPYYGDEDGTDGLYHSQAKRGTTAFHAYATLYARVKNKRYTLAVRRLEDGDTASSVLAEFLGILRRLDLDVKAVFLDREFYDSKCL
TLLQAHNHAYVMPIVRWGQTIKQELSEGWSRVIQHDMTAKLDGHSWTVEFLVYIDCTYQNGRCDEHGVARHGYAADAPFIESPRDARYHYAKRFGIEGSY
RLSEQSIATTSTQNPVVRLLYVVVSLLLQNVWRYLHWEYVRTPRRGGRRLWKWSFKEFINMIQRAAWTALATRRAVPANRPPDDRFTR
Blast result :
Comments
References
1] Pfeifer, F., Boyer, H.W., and Betlach, M.C. (1985) J. Bacteriol. 164, 414-420.
2] Pfeifer, F. (1986) System. Appl. Microbiol. 7, 36-40.
3] Pfeifer, F., Blaseio, U., and Ghahraman, P. (1988) J. Bacteriol. 170, 3718-3724.
4] Pfeifer, F., and Blaseio, U. (1989) J. Bacteriol. 171, 5135-5140.
5] Pfeifer, F., Blaseio, U., and Horne, M. (1989) Can. J. Microbiol. 35, 96-100.
6] Pfeifer, F., and Blaseio, U. (1990) Nucl. Acids Res. 18, 6921-6925.
7] Pfeifer, F., Horne, M., Englert, C., and Blaseio, U. (1991) in General and Applied Aspects of Halophilic Microorganisms (Rodriguez-Valera, F., Ed.) Plenum Press, New York, USA, pp. 285-293.
8] Pfeifer, F., and Englert, C. (1992) J. Bioenergetics and Biomembranes 24, 577-585.
9] Pfeifer, F., and Ghahraman, P. (1993) Mol. Gen. Genet. 238, 193-200
2] Pfeifer, F. (1986) System. Appl. Microbiol. 7, 36-40.
3] Pfeifer, F., Blaseio, U., and Ghahraman, P. (1988) J. Bacteriol. 170, 3718-3724.
4] Pfeifer, F., and Blaseio, U. (1989) J. Bacteriol. 171, 5135-5140.
5] Pfeifer, F., Blaseio, U., and Horne, M. (1989) Can. J. Microbiol. 35, 96-100.
6] Pfeifer, F., and Blaseio, U. (1990) Nucl. Acids Res. 18, 6921-6925.
7] Pfeifer, F., Horne, M., Englert, C., and Blaseio, U. (1991) in General and Applied Aspects of Halophilic Microorganisms (Rodriguez-Valera, F., Ed.) Plenum Press, New York, USA, pp. 285-293.
8] Pfeifer, F., and Englert, C. (1992) J. Bioenergetics and Biomembranes 24, 577-585.
9] Pfeifer, F., and Ghahraman, P. (1993) Mol. Gen. Genet. 238, 193-200