ISPa127
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa |
DNA section
IS Length : 1236 bp
Ends
IR Length : 22/25
IRL : TGTAGTGGTCTACTGATCCCGGACACCCATTTAGGGCGAGAATGCTCGCC
IRR : TGTAGTGGTCAACCCATCCCGGACAGTGGGTTAAGTTTTTCTTCGGCAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCC | 3 |
DNA sequence
TGTAGTGGTCTACTGATCCCGGACACCCATTTAGGGCGAGAATGCTCGCCATAGAGAGGTGTCTGATGACCAAGCAACGTCGTTCCTTTACGCCCGAGTT
CAAGCGAGAGGCCGCCGGCCTGGTGCTCGATCAGGGCTACAGCCATATCGAAGCAGCTCGCTCGCTGGGACTGGTTGAATCGGCGCTTCGTCGCTGGGTG
AAACAGCTCCAAGAAGAGCGCCAGGGTGTTACCCCGAAGAGCAAGGCATTGACTCCCGAACAGCAGAAAATCCAGGAGCTGGAAGCCAGGATCGACCGGC
TGGAACGGGAGAAAGCGATCCTAAAAAAGGCTACCGCTCTCTTGATGTCGGACGAGTTCAATCGTACGCGCTGATAGACCAGTTGAGTGAGCGGGAGTCG
GTGGAAGTGGTCTGTTCAGCCTTCGACGTGGTGCGGTCTTGCTACTACGCCCATCGCCTTCGACGCTGCCGTGTTGATGCACGCCGGGTTGCCCTGCGCA
GTCAGGTCAATCAACTGTTCAACCAGAGTCGAGGCTCGGCTGGCAGCCGCAGCATTCTGGGCATGTTGCGTGAGGATGGCGTAAGCATTGGCCGCTTTCG
CGTGCGCCGGTTGATGCGCGAGCTCGGCCTGGTCAGCAAGCAACCAGGCTCGCACGCATACAAGCAGGCTACGGTTGAGCGGCCAGATATCCCGAACCGG
TTGAACCGCGAGTTCACCCCCGAAAAGCCCAATCAGGTGTGGTGCGGCGACATCACCTACATCTGGGCGCAAGGTCGCTGGCATTACTTGGCGGCGGTGC
TGGATCTGCATGCCCGTCGAGTAATTGGCTGGGCATTTTCTCCCAAGCCGGATGCAGAGCTGGTGATCAAGGCGCTCGACATGGCGTATGAACAGCGAGG
CAAACCACAGCAGGTGCTGTTCCATTCCGATCAGGGCAGCCAATACGCCAGCCGCCTGTTTCGGCAGCGGTTGTGGCGATATCGGATGGAGCAGAGCATG
AGTCGCCGGGGCAACTGTTGGGATAACTCACCGATGGAGCGGTTATTCCGCAGTTTGAAATCCGAATGGATTCCGGCTACAGGCTACGTTACCGCTTCGG
AAGCTCAACGGGACATCAGTCATTACCTGATGCACCGTTACAACTGGGTCAGGCCACATCAATTCAACGACGGGCTACCGCCCGCCGTTGCCGAAGAAAA
ACTTAACCCACTGTCCGGGATGGGTTGACCACTACA
CAAGCGAGAGGCCGCCGGCCTGGTGCTCGATCAGGGCTACAGCCATATCGAAGCAGCTCGCTCGCTGGGACTGGTTGAATCGGCGCTTCGTCGCTGGGTG
AAACAGCTCCAAGAAGAGCGCCAGGGTGTTACCCCGAAGAGCAAGGCATTGACTCCCGAACAGCAGAAAATCCAGGAGCTGGAAGCCAGGATCGACCGGC
TGGAACGGGAGAAAGCGATCCTAAAAAAGGCTACCGCTCTCTTGATGTCGGACGAGTTCAATCGTACGCGCTGATAGACCAGTTGAGTGAGCGGGAGTCG
GTGGAAGTGGTCTGTTCAGCCTTCGACGTGGTGCGGTCTTGCTACTACGCCCATCGCCTTCGACGCTGCCGTGTTGATGCACGCCGGGTTGCCCTGCGCA
GTCAGGTCAATCAACTGTTCAACCAGAGTCGAGGCTCGGCTGGCAGCCGCAGCATTCTGGGCATGTTGCGTGAGGATGGCGTAAGCATTGGCCGCTTTCG
CGTGCGCCGGTTGATGCGCGAGCTCGGCCTGGTCAGCAAGCAACCAGGCTCGCACGCATACAAGCAGGCTACGGTTGAGCGGCCAGATATCCCGAACCGG
TTGAACCGCGAGTTCACCCCCGAAAAGCCCAATCAGGTGTGGTGCGGCGACATCACCTACATCTGGGCGCAAGGTCGCTGGCATTACTTGGCGGCGGTGC
TGGATCTGCATGCCCGTCGAGTAATTGGCTGGGCATTTTCTCCCAAGCCGGATGCAGAGCTGGTGATCAAGGCGCTCGACATGGCGTATGAACAGCGAGG
CAAACCACAGCAGGTGCTGTTCCATTCCGATCAGGGCAGCCAATACGCCAGCCGCCTGTTTCGGCAGCGGTTGTGGCGATATCGGATGGAGCAGAGCATG
AGTCGCCGGGGCAACTGTTGGGATAACTCACCGATGGAGCGGTTATTCCGCAGTTTGAAATCCGAATGGATTCCGGCTACAGGCTACGTTACCGCTTCGG
AAGCTCAACGGGACATCAGTCATTACCTGATGCACCGTTACAACTGGGTCAGGCCACATCAATTCAACGACGGGCTACCGCCCGCCGTTGCCGAAGAAAA
ACTTAACCCACTGTCCGGGATGGGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 66 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MTKQRRSFTPEFKREAAGLVLDQGYSHIEAARSLGLVESALRRWVKQLQEERQGVTPKSKALTPEQQKIQELEARIDRLEREKAILKKATALLMSDEFNR
TR
TR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1050 bp | 349 aa | 179 | 1228 | + | No |
Description : Second part of the transposase
ORF sequence :
IGASSLGETAPRRAPGCYPEEQGIDSRTAENPGAGSQDRPAGTGESDPKKGYRSLDVGRVQSYALIDQLSERESVEVVCSAFDVVRSCYYAHRLRRCRVD
ARRVALRSQVNQLFNQSRGSAGSRSILGMLREDGVSIGRFRVRRLMRELGLVSKQPGSHAYKQATVERPDIPNRLNREFTPEKPNQVWCGDITYIWAQGR
WHYLAAVLDLHARRVIGWAFSPKPDAELVIKALDMAYEQRGKPQQVLFHSDQGSQYASRLFRQRLWRYRMEQSMSRRGNCWDNSPMERLFRSLKSEWIPA
TGYVTASEAQRDISHYLMHRYLNPLSGMGNWVRPHQFNDGLPPAVAEEK
ARRVALRSQVNQLFNQSRGSAGSRSILGMLREDGVSIGRFRVRRLMRELGLVSKQPGSHAYKQATVERPDIPNRLNREFTPEKPNQVWCGDITYIWAQGR
WHYLAAVLDLHARRVIGWAFSPKPDAELVIKALDMAYEQRGKPQQVLFHSDQGSQYASRLFRQRLWRYRMEQSMSRRGNCWDNSPMERLFRSLKSEWIPA
TGYVTASEAQRDISHYLMHRYLNPLSGMGNWVRPHQFNDGLPPAVAEEK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 66 | 1228 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKQRRSFTPEFKREAAGLVLDQGYSHIEAARSLGLVESALRRWVKQLQEERQGVTPKSKALTPEQQKIQELEARIDRLEREKAILKKGYRSLDVGRVQS
YALIDQLSERESVEVVCSAFDVVRSCYYAHRLRRCRVDARRVALRSQVNQLFNQSRGSAGSRSILGMLREDGVSIGRFRVRRLMRELGLVSKQPGSHAYK
QATVERPDIPNRLNREFTPEKPNQVWCGDITYIWAQGRWHYLAAVLDLHARRVIGWAFSPKPDAELVIKALDMAYEQRGKPQQVLFHSDQGSQYASRLFR
QRLWRYRMEQSMSRRGNCWDNSPMERLFRSLKSEWIPATGYVTASEAQRDISHYLMHRYLNPLSGMGNWVRPHQFNDGLPPAVAEEK
YALIDQLSERESVEVVCSAFDVVRSCYYAHRLRRCRVDARRVALRSQVNQLFNQSRGSAGSRSILGMLREDGVSIGRFRVRRLMRELGLVSKQPGSHAYK
QATVERPDIPNRLNREFTPEKPNQVWCGDITYIWAQGRWHYLAAVLDLHARRVIGWAFSPKPDAELVIKALDMAYEQRGKPQQVLFHSDQGSQYASRLFR
QRLWRYRMEQSMSRRGNCWDNSPMERLFRSLKSEWIPATGYVTASEAQRDISHYLMHRYLNPLSGMGNWVRPHQFNDGLPPAVAEEK
Blast result :
Comments
ISPa127 is 94% aa (transposase) similar to IS222.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.