ISPa121
- Family IS3
- Group IS3
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP050332 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas aeruginosa DVT413 |
DNA section
IS Length : 1251 bp
Ends
IR Length : 23/26
IRL : TGGTCTGTCCCGGGCTGAGTGGACACTGACTTAAGGTGGATAATGCCCAC
IRR : TGGTCTGTCCCGGAACTGGTGGACAGGTAGTTAAGAGTTACTGCTCGCAT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CAAGAAATAT | GTCTGTATTC | 0 |
DNA sequence
TGGTCTGTCCCGGGCTGAGTGGACACTGACTTAAGGTGGATAATGCCCACCGCAAGGAGTGTTCATGACCCAGACCAGACGACGTTTTCCCGAATCCTTC
AAACGCGAGGCGGTAGACCAGGTGCTTGCCGGTACGCCGCTTCGTCATGTCGCCGAGACACTCGGCATTGCTGAAAGCCTGCTGGGCAAATGGAAGCGCC
AGTACGAGCAGCAAGGTGACGATGCCTTTCCGGGCAACGGCAAGCAGCGTGGCGAGAGCGCGGAGCTTCGTCGCCTGCGCCGGCAACTGGCCCAGGTCAC
CATGGAGCGCGATGTTTTAAAAAAGGCGCTCGCCATCTTCTCGCAGCCCACGAAGTGAAGTTTCGCGCCATCGAGGCGTTGGCTGGTCGTTACCCCGTAG
CACCGATGTGCCGGTTGTTGCAGGTGTCGCGCAGCGGTTTCTATGCCTGGCAACAGCGACCACCTTCGGTGAGAGAGATGGCGAATCGGCGTCTGAGCAA
GGAGATCCGAACCATCCACCACGAGGTGAATGGGATCTATGGTCATCGCCGCATCAAGGCTGAACTGACGGCCATGGGGCAGGCGTGCGGGCGGCATCGG
GTTGCTCGATTGATGCGCGAAGCCGGCCTGCGTGTTCGTTCACGCAAGCGCTGGCGGCTGGTTTCCAGTAGCCGCCACGATCTACCGATTGCGCCGAATC
ACCTGGATCGCCAGTTCGTCTCGGATCGAGCCAATCGGCACTGGGTCTCGGACATGACCTACGTGCGGACTGCGCAAGGCTGGCTGTATCTGGCCGTTGT
GCTCGATCTCTATTCACGCGCCGTGGTCGGTTGGGCGATGCACCACCGCATGCAGCAAGCCCTGGTGCACGCCGCGCTGGAGATGGCCGTGGCACGCCGA
CAGCCACAGGCGGAGGTCCTGCTGCACTCGGATCGCGGCAGCCAATACTGCGCGTACGACTACCAAGCCTTGCTTCGGCGACATCGCATCGTGCCCAGCC
ATTCGCGACCAGGGAACTGCTGGGATAACGCGGCTATGGAGAGCTTTTTCCGTTCGCTGAAGGCCGAGCGGGTGTACCTGACGCGCTATGCCAGCTACCA
GGAGGCGAAAACCGACCTGTTCGACTACATCCGCTTTTACAATCACCGTCGTCGCCACTCGACGCTGGGCTATCTCAGCCCGATGGAGTTTGAGCGGCGC
TATGCGAGCAGTAACTCTTAACTACCTGTCCACCAGTTCCGGGACAGACCA
AAACGCGAGGCGGTAGACCAGGTGCTTGCCGGTACGCCGCTTCGTCATGTCGCCGAGACACTCGGCATTGCTGAAAGCCTGCTGGGCAAATGGAAGCGCC
AGTACGAGCAGCAAGGTGACGATGCCTTTCCGGGCAACGGCAAGCAGCGTGGCGAGAGCGCGGAGCTTCGTCGCCTGCGCCGGCAACTGGCCCAGGTCAC
CATGGAGCGCGATGTTTTAAAAAAGGCGCTCGCCATCTTCTCGCAGCCCACGAAGTGAAGTTTCGCGCCATCGAGGCGTTGGCTGGTCGTTACCCCGTAG
CACCGATGTGCCGGTTGTTGCAGGTGTCGCGCAGCGGTTTCTATGCCTGGCAACAGCGACCACCTTCGGTGAGAGAGATGGCGAATCGGCGTCTGAGCAA
GGAGATCCGAACCATCCACCACGAGGTGAATGGGATCTATGGTCATCGCCGCATCAAGGCTGAACTGACGGCCATGGGGCAGGCGTGCGGGCGGCATCGG
GTTGCTCGATTGATGCGCGAAGCCGGCCTGCGTGTTCGTTCACGCAAGCGCTGGCGGCTGGTTTCCAGTAGCCGCCACGATCTACCGATTGCGCCGAATC
ACCTGGATCGCCAGTTCGTCTCGGATCGAGCCAATCGGCACTGGGTCTCGGACATGACCTACGTGCGGACTGCGCAAGGCTGGCTGTATCTGGCCGTTGT
GCTCGATCTCTATTCACGCGCCGTGGTCGGTTGGGCGATGCACCACCGCATGCAGCAAGCCCTGGTGCACGCCGCGCTGGAGATGGCCGTGGCACGCCGA
CAGCCACAGGCGGAGGTCCTGCTGCACTCGGATCGCGGCAGCCAATACTGCGCGTACGACTACCAAGCCTTGCTTCGGCGACATCGCATCGTGCCCAGCC
ATTCGCGACCAGGGAACTGCTGGGATAACGCGGCTATGGAGAGCTTTTTCCGTTCGCTGAAGGCCGAGCGGGTGTACCTGACGCGCTATGCCAGCTACCA
GGAGGCGAAAACCGACCTGTTCGACTACATCCGCTTTTACAATCACCGTCGTCGCCACTCGACGCTGGGCTATCTCAGCCCGATGGAGTTTGAGCGGCGC
TATGCGAGCAGTAACTCTTAACTACCTGTCCACCAGTTCCGGGACAGACCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
294 bp | 97 aa | 65 | 358 | + | No |
Description : First part of the transposase
ORF sequence :
MTQTRRRFPESFKREAVDQVLAGTPLRHVAETLGIAESLLGKWKRQYEQQGDDAFPGNGKQRGESAELRRLRRQLAQVTMERDVLKKALAIFSQPTK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1002 bp | 333 aa | 220 | 1221 | + | No |
Description : Second part of the transposase
ORF sequence :
RCLSGQRQAAWRERGASSPAPATGPGHHGARCFKKGARHLLAAHEVKFRAIEALAGRYPVAPMCRLLQVSRSGFYAWQQRPPSVREMANRRLSKEIRTIH
HEVNGIYGHRRIKAELTAMGQACGRHRVARLMREAGLRVRSRKRWRLVSSSRHDLPIAPNHLDRQFVSDRANRHWVSDMTYVRTAQGWLYLAVVLDLYSR
AVVGWAMHHRMQQALVHAALEMAVARRQPQAEVLLHSDRGSQYCAYDYQALLRRHRIVPSHSRPGNCWDNAAMESFFRSLKAERVYLTRYASYQEAKTDL
FDYIRFYNHRRRHSTLGYLSPMEFERRYASSNS
HEVNGIYGHRRIKAELTAMGQACGRHRVARLMREAGLRVRSRKRWRLVSSSRHDLPIAPNHLDRQFVSDRANRHWVSDMTYVRTAQGWLYLAVVLDLYSR
AVVGWAMHHRMQQALVHAALEMAVARRQPQAEVLLHSDRGSQYCAYDYQALLRRHRIVPSHSRPGNCWDNAAMESFFRSLKAERVYLTRYASYQEAKTDL
FDYIRFYNHRRRHSTLGYLSPMEFERRYASSNS
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1157 bp | 385 aa | 65 | 1221 | + | Yes |
Chemistry : DDE
ORF sequence :
MTQTRRRFPESFKREAVDQVLAGTPLRHVAETLGIAESLLGKWKRQYEQQGDDAFPGNGKQRGESAELRRLRRQLAQVTMERDVLKKGARHLLAAHEVKF
RAIEALAGRYPVAPMCRLLQVSRSGFYAWQQRPPSVREMANRRLSKEIRTIHHEVNGIYGHRRIKAELTAMGQACGRHRVARLMREAGLRVRSRKRWRLV
SSSRHDLPIAPNHLDRQFVSDRANRHWVSDMTYVRTAQGWLYLAVVLDLYSRAVVGWAMHHRMQQALVHAALEMAVARRQPQAEVLLHSDRGSQYCAYDY
QALLRRHRIVPSHSRPGNCWDNAAMESFFRSLKAERVYLTRYASYQEAKTDLFDYIRFYNHRRRHSTLGYLSPMEFERRYASSNS
RAIEALAGRYPVAPMCRLLQVSRSGFYAWQQRPPSVREMANRRLSKEIRTIHHEVNGIYGHRRIKAELTAMGQACGRHRVARLMREAGLRVRSRKRWRLV
SSSRHDLPIAPNHLDRQFVSDRANRHWVSDMTYVRTAQGWLYLAVVLDLYSRAVVGWAMHHRMQQALVHAALEMAVARRQPQAEVLLHSDRGSQYCAYDY
QALLRRHRIVPSHSRPGNCWDNAAMESFFRSLKAERVYLTRYASYQEAKTDLFDYIRFYNHRRRHSTLGYLSPMEFERRYASSNS
Blast result :
Comments
ISPa121 is 64% aa (transposase) similar to ISHahy5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible-1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible-1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Evans,D.R., Nordstrom,H.R., Finney,A.G., Westbrook,K.J., Iovleva,A., Guerrero-Bustamante,C.A., Yassin,M.H., Shields,R.K., Doi,Y. and Van Tyne,D. (2020) Direct GenBank submission.
2] Evans,D.R., Nordstrom,H.R., Finney,A.G., Westbrook,K.J., Iovleva,A., Guerrero-Bustamante,C.A., Yassin,M.H., Shields,R.K., Doi,Y. and Van Tyne,D. (2020) Direct GenBank submission.