ISPa121

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP050332 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa DVT413
DNA section
IS Length : 1251 bp

Ends


IR Length : 23/26

IRL : TGGTCTGTCCCGGGCTGAGTGGACACTGACTTAAGGTGGATAATGCCCAC
IRR : TGGTCTGTCCCGGAACTGGTGGACAGGTAGTTAAGAGTTACTGCTCGCAT

Insertion site


Left flankDirect repeatRight flankDR Length
CAAGAAATATGTCTGTATTC0

DNA sequence

TGGTCTGTCCCGGGCTGAGTGGACACTGACTTAAGGTGGATAATGCCCACCGCAAGGAGTGTTCATGACCCAGACCAGACGACGTTTTCCCGAATCCTTC
AAACGCGAGGCGGTAGACCAGGTGCTTGCCGGTACGCCGCTTCGTCATGTCGCCGAGACACTCGGCATTGCTGAAAGCCTGCTGGGCAAATGGAAGCGCC
AGTACGAGCAGCAAGGTGACGATGCCTTTCCGGGCAACGGCAAGCAGCGTGGCGAGAGCGCGGAGCTTCGTCGCCTGCGCCGGCAACTGGCCCAGGTCAC
CATGGAGCGCGATGTTTTAAAAAAGGCGCTCGCCATCTTCTCGCAGCCCACGAAGTGAAGTTTCGCGCCATCGAGGCGTTGGCTGGTCGTTACCCCGTAG
CACCGATGTGCCGGTTGTTGCAGGTGTCGCGCAGCGGTTTCTATGCCTGGCAACAGCGACCACCTTCGGTGAGAGAGATGGCGAATCGGCGTCTGAGCAA
GGAGATCCGAACCATCCACCACGAGGTGAATGGGATCTATGGTCATCGCCGCATCAAGGCTGAACTGACGGCCATGGGGCAGGCGTGCGGGCGGCATCGG
GTTGCTCGATTGATGCGCGAAGCCGGCCTGCGTGTTCGTTCACGCAAGCGCTGGCGGCTGGTTTCCAGTAGCCGCCACGATCTACCGATTGCGCCGAATC
ACCTGGATCGCCAGTTCGTCTCGGATCGAGCCAATCGGCACTGGGTCTCGGACATGACCTACGTGCGGACTGCGCAAGGCTGGCTGTATCTGGCCGTTGT
GCTCGATCTCTATTCACGCGCCGTGGTCGGTTGGGCGATGCACCACCGCATGCAGCAAGCCCTGGTGCACGCCGCGCTGGAGATGGCCGTGGCACGCCGA
CAGCCACAGGCGGAGGTCCTGCTGCACTCGGATCGCGGCAGCCAATACTGCGCGTACGACTACCAAGCCTTGCTTCGGCGACATCGCATCGTGCCCAGCC
ATTCGCGACCAGGGAACTGCTGGGATAACGCGGCTATGGAGAGCTTTTTCCGTTCGCTGAAGGCCGAGCGGGTGTACCTGACGCGCTATGCCAGCTACCA
GGAGGCGAAAACCGACCTGTTCGACTACATCCGCTTTTACAATCACCGTCGTCGCCACTCGACGCTGGGCTATCTCAGCCCGATGGAGTTTGAGCGGCGC
TATGCGAGCAGTAACTCTTAACTACCTGTCCACCAGTTCCGGGACAGACCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
294 bp97 aa65358+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTQTRRRFPESFKREAVDQVLAGTPLRHVAETLGIAESLLGKWKRQYEQQGDDAFPGNGKQRGESAELRRLRRQLAQVTMERDVLKKALAIFSQPTK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1002 bp333 aa2201221+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RCLSGQRQAAWRERGASSPAPATGPGHHGARCFKKGARHLLAAHEVKFRAIEALAGRYPVAPMCRLLQVSRSGFYAWQQRPPSVREMANRRLSKEIRTIH
HEVNGIYGHRRIKAELTAMGQACGRHRVARLMREAGLRVRSRKRWRLVSSSRHDLPIAPNHLDRQFVSDRANRHWVSDMTYVRTAQGWLYLAVVLDLYSR
AVVGWAMHHRMQQALVHAALEMAVARRQPQAEVLLHSDRGSQYCAYDYQALLRRHRIVPSHSRPGNCWDNAAMESFFRSLKAERVYLTRYASYQEAKTDL
FDYIRFYNHRRRHSTLGYLSPMEFERRYASSNS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1157 bp385 aa651221+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTQTRRRFPESFKREAVDQVLAGTPLRHVAETLGIAESLLGKWKRQYEQQGDDAFPGNGKQRGESAELRRLRRQLAQVTMERDVLKKGARHLLAAHEVKF
RAIEALAGRYPVAPMCRLLQVSRSGFYAWQQRPPSVREMANRRLSKEIRTIHHEVNGIYGHRRIKAELTAMGQACGRHRVARLMREAGLRVRSRKRWRLV
SSSRHDLPIAPNHLDRQFVSDRANRHWVSDMTYVRTAQGWLYLAVVLDLYSRAVVGWAMHHRMQQALVHAALEMAVARRQPQAEVLLHSDRGSQYCAYDY
QALLRRHRIVPSHSRPGNCWDNAAMESFFRSLKAERVYLTRYASYQEAKTDLFDYIRFYNHRRRHSTLGYLSPMEFERRYASSNS

 

Blast result :
Comments
ISPa121 is 64% aa (transposase) similar to ISHahy5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible-1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Evans,D.R., Nordstrom,H.R., Finney,A.G., Westbrook,K.J., Iovleva,A., Guerrero-Bustamante,C.A., Yassin,M.H., Shields,R.K., Doi,Y. and Van Tyne,D. (2020) Direct GenBank submission.