ISPa99
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Escherichia coli 47EC plasmid p47EC |
DNA section
IS Length : 1325 bp
Ends
IR Length : 18/20
IRL : CTGAGGTATCCCCAGAAATTTTGTAGTTAAAATAAAAATTTAGGTAATTA
IRR : CTGAGGGATCCCCACAAATTGCTCCCCCATATTATCCACACTCTATCAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| T | CTAACTCAC | G | 9 |
| CTGAATAATGT | ATAATGGCT | TGTTTTTTCTATT | 9 |
DNA sequence
CTGAGGTATCCCCAGAAATTTTGTAGTTAAAATAAAAATTTAGGTAATTAGGGAACATTCATGGCGGTTCCCGATCTGATCATTCGCCAAAAATCCCACA
CCGCGGATGCTCGCCATGAATGTTAAAACTATGCTCGCTGATTTCCTCCGATTTGTCACCCCAAAAACCATGCATAAATCCAGATTTTCTGTCTTGTTAG
ATGCGGTTACTGCTCTTGCTAAAGACGCTCGTTGCACCGTTACAGCAATTGGTCGCGCTATGCCAGGGCCCTCAGACAAAATCAGCATCAAGCGAGCCGA
CAGATTACTCAATAATGCCAATCTGCAACGAGAGCTACCGTTGATTTATGCCGCGTTGACTGCTTCTGTTGTAACCCCCCATTCCCATCCAACGATTTTA
GTCGATTGGAGTAATGCGGATACCGCCAAGCGTCACTTTATTTTACGAGCGAGTATTGCTGCGGACGGCAGAGCATTAACGTTGTTGCAAAAAATCGCTG
CTGAAGAAGAGTACACCAGCCCCCGTTTGCATCAGGCATTCTTAGAACAACTTAAAGCTATGTTACCAGAAGGCTGCAAGCCAATTATCGTGACAGACGC
CGGATTTAAAGTGCCTTGGTTAAAACAAGTACGCGAATTCGGCTGGCATTATGTCGCCAGAGTCAGAGGGAATGTGAAGCTGAAAGTGGCAGAGCAGGAA
GAGTTTATCACTGTTAACCAATTTTATCGTAAGGCCAAAGCCAAGCCCAAGCATGTCGGCAACATCTTACTCACCCAATCTCAGAGCTATGAAACACAAG
CTGTTTTGGTTGGAAAAGGTTACAAGTTATTGAAAAGAGATAAAAACAGGCAATACAAAGAGCCTTGGCTATTAGTTTCCTCTTTACCAGAAAGTCATGG
ATATGCCGAGAAAATTGCCAAATGCTATAGCTGCCGAATGCAGATAGAAGAAAGCTTCCGGGATCAAAAAAGTCACCGTTACGGGTTAGGTAGCGATCTA
CATGGTACTCGCAAGAAATCACGCTTGGAGATCTTACTGCTCTTGGCAGCCCTGGCTAACTGGTTTCATTACCTGCTTGGCAGTGCGGGTGAGAATGCTG
GCTTACAACGACGTTATCAGGCTAATACCGTTAAGCATAGACGAGTGCTCGCTCTTTGTTCCCTTGGGATACTGCTCTGTAAAGAGCAAAAGCTTAATAT
AAGTAGGCGATATTATCAGCAAGGGCTAAAGCAAATTTTGCAGTGGATAGCTCAGTGGTGCTGGACACCGGACGATCTGATAGAGTGTGGATAATATGGG
GGAGCAATTTGTGGGGATCCCTCAG
CCGCGGATGCTCGCCATGAATGTTAAAACTATGCTCGCTGATTTCCTCCGATTTGTCACCCCAAAAACCATGCATAAATCCAGATTTTCTGTCTTGTTAG
ATGCGGTTACTGCTCTTGCTAAAGACGCTCGTTGCACCGTTACAGCAATTGGTCGCGCTATGCCAGGGCCCTCAGACAAAATCAGCATCAAGCGAGCCGA
CAGATTACTCAATAATGCCAATCTGCAACGAGAGCTACCGTTGATTTATGCCGCGTTGACTGCTTCTGTTGTAACCCCCCATTCCCATCCAACGATTTTA
GTCGATTGGAGTAATGCGGATACCGCCAAGCGTCACTTTATTTTACGAGCGAGTATTGCTGCGGACGGCAGAGCATTAACGTTGTTGCAAAAAATCGCTG
CTGAAGAAGAGTACACCAGCCCCCGTTTGCATCAGGCATTCTTAGAACAACTTAAAGCTATGTTACCAGAAGGCTGCAAGCCAATTATCGTGACAGACGC
CGGATTTAAAGTGCCTTGGTTAAAACAAGTACGCGAATTCGGCTGGCATTATGTCGCCAGAGTCAGAGGGAATGTGAAGCTGAAAGTGGCAGAGCAGGAA
GAGTTTATCACTGTTAACCAATTTTATCGTAAGGCCAAAGCCAAGCCCAAGCATGTCGGCAACATCTTACTCACCCAATCTCAGAGCTATGAAACACAAG
CTGTTTTGGTTGGAAAAGGTTACAAGTTATTGAAAAGAGATAAAAACAGGCAATACAAAGAGCCTTGGCTATTAGTTTCCTCTTTACCAGAAAGTCATGG
ATATGCCGAGAAAATTGCCAAATGCTATAGCTGCCGAATGCAGATAGAAGAAAGCTTCCGGGATCAAAAAAGTCACCGTTACGGGTTAGGTAGCGATCTA
CATGGTACTCGCAAGAAATCACGCTTGGAGATCTTACTGCTCTTGGCAGCCCTGGCTAACTGGTTTCATTACCTGCTTGGCAGTGCGGGTGAGAATGCTG
GCTTACAACGACGTTATCAGGCTAATACCGTTAAGCATAGACGAGTGCTCGCTCTTTGTTCCCTTGGGATACTGCTCTGTAAAGAGCAAAAGCTTAATAT
AAGTAGGCGATATTATCAGCAAGGGCTAAAGCAAATTTTGCAGTGGATAGCTCAGTGGTGCTGGACACCGGACGATCTGATAGAGTGTGGATAATATGGG
GGAGCAATTTGTGGGGATCCCTCAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1179 bp | 392 aa | 116 | 1294 | + | No |
Chemistry : DDE
ORF sequence :
MNVKTMLADFLRFVTPKTMHKSRFSVLLDAVTALAKDARCTVTAIGRAMPGPSDKISIKRADRLLNNANLQRELPLIYAALTASVVTPHSHPTILVDWSN
ADTAKRHFILRASIAADGRALTLLQKIAAEEEYTSPRLHQAFLEQLKAMLPEGCKPIIVTDAGFKVPWLKQVREFGWHYVARVRGNVKLKVAEQEEFITV
NQFYRKAKAKPKHVGNILLTQSQSYETQAVLVGKGYKLLKRDKNRQYKEPWLLVSSLPESHGYAEKIAKCYSCRMQIEESFRDQKSHRYGLGSDLHGTRK
KSRLEILLLLAALANWFHYLLGSAGENAGLQRRYQANTVKHRRVLALCSLGILLCKEQKLNISRRYYQQGLKQILQWIAQWCWTPDDLIECG
ADTAKRHFILRASIAADGRALTLLQKIAAEEEYTSPRLHQAFLEQLKAMLPEGCKPIIVTDAGFKVPWLKQVREFGWHYVARVRGNVKLKVAEQEEFITV
NQFYRKAKAKPKHVGNILLTQSQSYETQAVLVGKGYKLLKRDKNRQYKEPWLLVSSLPESHGYAEKIAKCYSCRMQIEESFRDQKSHRYGLGSDLHGTRK
KSRLEILLLLAALANWFHYLLGSAGENAGLQRRYQANTVKHRRVLALCSLGILLCKEQKLNISRRYYQQGLKQILQWIAQWCWTPDDLIECG
Blast result :
Comments
ISPa99 is 90% aa similar to ISEc29.
Accession number of the E. coli sequence : MK134376
Accession number of the E. coli sequence : MK134376
References
1] Dongsheng Zhou (2020) Direct submission.
2] He,T., Wang,R., Liu,D., Walsh,T.R., Zhang,R., Lv,Y., Ke,Y., Ji,Q., Wei,R., Liu,Z., Shen,Y., Sun,L., Lei,L., Lv,Z., Li,Y., Pang,M., Wang,L., Sun,Q., Shen,Z., Wang,S., Chen,G., Wu,C., Shen,J. and Wang,Y. (2018) Direct GenBank submission.
2] He,T., Wang,R., Liu,D., Walsh,T.R., Zhang,R., Lv,Y., Ke,Y., Ji,Q., Wei,R., Liu,Z., Shen,Y., Sun,L., Lei,L., Lv,Z., Li,Y., Pang,M., Wang,L., Sun,Q., Shen,Z., Wang,S., Chen,G., Wu,C., Shen,J. and Wang,Y. (2018) Direct GenBank submission.