ISLead3
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MN423365 | ND | Leclercia adcarboxglata | Leclercia adcarboxglata Leclercia adecarboxylata L21 |
DNA section
IS Length : 764 bp
Ends
IR Length : 18/23
IRL : GGTCATGACGCCAACTTAGTGCTGCTGTATACTTCGCCTTTTGGATAAGT
IRR : GGTCGTGACGCTGATTTAGTGGTAATGGTTGATTGTCAGATACCATCCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATATTACTACAA | CAGATTTG | CGTTATTGTTAT | 8 |
DNA sequence
GGTCATGACGCCAACTTAGTGCTGCTGTATACTTCGCCTTTTGGATAAGTGATGGCAAAGATTGATGTGGTTTGTCCCCGTTGCTCTGAAACTCAAGGGG
TTATCCGTAATGGTCACTCAACGTCAGGTGCTCAGCTCTATCGCTGCAAGCTCTGCCTGAAAACCTTCCAGCTCAGCTTTCAGTACAACGCCGCTAAACC
CGATACCCATCAATCCATTATTGATATGGCAATGAATGGGGCCGGGTGTCGTGACACTGCACGAGTCCTTGATATCAGCCTCAATACTGTCCTCCGCCAT
CTAAAAAACTGGCGCCGAAACAGGTAGCGCAACACATTGAGCCCGGAGCAGAAGTTGTCATCTGTTGTGAAGCTGATGAGCAGTGGTCATTCGTACGTTG
TAAGGGCAATCCACGCTGGCTCTTTTATGCCTATGACCGCATCCGAAAGCGGGTTCTTGCTCACGTCTTTGGCCCCCGAAATGCGGTGACGTTACAGCGG
CTTCTGGCACTGCTGAGCAAATTTAATATTGCCTTCTACATGACCGATGCGTGGCCGGTATACCGGACGTTGCTGGACTCAGCCAGCCATGTAGTCAGCA
AGAAATATACCCAGCGAATAGAGCGTCATAACCTGAATCTGCGGACTCACCTTAAGCGCCTTACCCGAAGGACAATCTGCTTTTCAAAATCAGAAGAAAT
GCACGATAAGGTCATCGGATGGTATCTGACAATCAACCATTACCACTAAATCAGCGTCACGACC
TTATCCGTAATGGTCACTCAACGTCAGGTGCTCAGCTCTATCGCTGCAAGCTCTGCCTGAAAACCTTCCAGCTCAGCTTTCAGTACAACGCCGCTAAACC
CGATACCCATCAATCCATTATTGATATGGCAATGAATGGGGCCGGGTGTCGTGACACTGCACGAGTCCTTGATATCAGCCTCAATACTGTCCTCCGCCAT
CTAAAAAACTGGCGCCGAAACAGGTAGCGCAACACATTGAGCCCGGAGCAGAAGTTGTCATCTGTTGTGAAGCTGATGAGCAGTGGTCATTCGTACGTTG
TAAGGGCAATCCACGCTGGCTCTTTTATGCCTATGACCGCATCCGAAAGCGGGTTCTTGCTCACGTCTTTGGCCCCCGAAATGCGGTGACGTTACAGCGG
CTTCTGGCACTGCTGAGCAAATTTAATATTGCCTTCTACATGACCGATGCGTGGCCGGTATACCGGACGTTGCTGGACTCAGCCAGCCATGTAGTCAGCA
AGAAATATACCCAGCGAATAGAGCGTCATAACCTGAATCTGCGGACTCACCTTAAGCGCCTTACCCGAAGGACAATCTGCTTTTCAAAATCAGAAGAAAT
GCACGATAAGGTCATCGGATGGTATCTGACAATCAACCATTACCACTAAATCAGCGTCACGACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 52 | 327 | + | No |
Description : First part of the transposase
ORF sequence :
MAKIDVVCPRCSETQGVIRNGHSTSGAQLYRCKLCLKTFQLSFQYNAAKPDTHQSIIDMAMNGAGCRDTARVLDISLNTVLRHLKNWRRNR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 477 bp | 158 aa | 273 | 749 | + | No |
Description : Second part of the transposase
ORF sequence :
YQPQYCPPPSKKLAPKQVAQHIEPGAEVVICCEADEQWSFVRCKGNPRWLFYAYDRIRKRVLAHVFGPRNAVTLQRLLALLSKFNIAFYMTDAWPVYRTL
LDSASHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKSEEMHDKVIGWYLTINHYH
LDSASHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKSEEMHDKVIGWYLTINHYH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 698 bp | 232 aa | 52 | 749 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKIDVVCPRCSETQGVIRNGHSTSGAQLYRCKLCLKTFQLSFQYNAAKPDTHQSIIDMAMNGAGCRDTARVLDISLNTVLRHLKKLAPKQVAQHIEPGA
EVVICCEADEQWSFVRCKGNPRWLFYAYDRIRKRVLAHVFGPRNAVTLQRLLALLSKFNIAFYMTDAWPVYRTLLDSASHVVSKKYTQRIERHNLNLRTH
LKRLTRRTICFSKSEEMHDKVIGWYLTINHYH
EVVICCEADEQWSFVRCKGNPRWLFYAYDRIRKRVLAHVFGPRNAVTLQRLLALLSKFNIAFYMTDAWPVYRTLLDSASHVVSKKYTQRIERHNLNLRTH
LKRLTRRTICFSKSEEMHDKVIGWYLTINHYH
Blast result :
Comments
ISLead3 is 97% aa (transposase) similar to ISEic1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Yanan Xu (2020) Direct submission.
2] Zhou,D.S. and Zeng,L. (2019) Direct submission.
2] Zhou,D.S. and Zeng,L. (2019) Direct submission.