ISH26
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X04832 | Y | Halobacterium salinarum | Halobacterium tunesiensis Halobacterium sp. SB3 Halobacterium salinarum PHH1 plasmid pHH1 Halobacterium sp. GN Halobacterium sp. RS1 |
DNA section
IS Length : 1384 bp
Ends
IR Length : 19
IRL : CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGCGGCTTATCGAG
IRR : CATCCGTCTTTAGTTAAGCCTCACACCTCGGTTGTGTAGTGGTAGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCTGGGGTA | TGGCCTCACAA | TGGTACNNNN | 11 |
| TTCTTGGTTA | AGTTTGTCGGC | AGTCTGGCGT | 11 |
DNA sequence
CATCCGTCTTTAGTTAAGCGAGAAACCGCGTGATAGCGCGGCTTATCGAGGCATCTTTCGGAAGGAATGAGGAAGTCCAGTCTGTGCACGACAAGGACTC
CTCACCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTCGAAGACCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCTC
CAGATCCCTGCCTTCGTTTGGGCGTTCGTGTTCGGCTTCGCGCAGGAACAGCGAACACTCGCCGGTTTTAGACGCTGTTACAACTCTACTGCCGATGAGA
CGATTTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTCGAGCATGGTCTCGACGAGGTCGCCGTTCCTAA
CGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGCCCGC
CACGAGGAGCAGGCTGGAGCGAAGTCACCTGCCTCACAATGCCACAGAGCAGACGATTGAACAGCTCGATACTGCTAACGAGAAAACGCACGACAGCACC
TTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCATGCTGTTCGATCTCGCCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAATGACGGC
TACGTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACCGGAGAGCTACGAATCGCGGCCGCGCCATTCCCTTAGAGGGCAAGCAGCTCCGAG
CTGTTCTCGATGATCTTGACCGGAAATACATCGATGTGGAGGTCGAAGTCGAATTCCAAACGGGGGCCGTACAATGGGACACAGTCGCTGGATACGAAGC
GGTTTCGCGTCGTCGGCGTCCGCGACGAGGACGCCGACGACTACCACCTGTACATGACGAATTTAGCGAGGAAAGAGTTCTTTCCGGCGGATTTAGCGGA
GATCTACCGTGTCGGTGGGAAGTTGAGTTGCTGTTCCGGGAGCTGAAGACGCAGTACGAATTGGACGAGTTCGACACGAGTGACGAACACGTGGTGAGGA
TCTTATTGTACGCAGCGCTGCTGTCGCTGCTTGTAAGCCGCGACTCGTTGGATCTAGTCACTGAGCAGGCGGATGATGAGCGTTGTGTTTCCGACAGACC
TGCGACCGGTCGCACGCCCAGCTTATTCTCCTCGAACTCGGTGAGTTCCTTGGCTACTCACCACCGCCGCTTCTCGACCGGCTGATCGAAGACGCTCAAA
AGATCCACAAGCAACGACCAATCTTACAAGAGACGCTCGCTACCACTACACAACCGAGGTGTGAGGCTTAACTAAAGACGGATG
CTCACCTCGGATTATGCGTCGGCTCACTACACTGTTTCCCTCTGAGTTCCTCGAAGACCACGCCGAGGAACTCGGCGTGGTCGAACGTGACCGCAAGCTC
CAGATCCCTGCCTTCGTTTGGGCGTTCGTGTTCGGCTTCGCGCAGGAACAGCGAACACTCGCCGGTTTTAGACGCTGTTACAACTCTACTGCCGATGAGA
CGATTTCTCCGGGTGGATTCTATCAGCGGTTGACACCGACGCTTGCGGAGTATCTCCGCGACCTCGTCGAGCATGGTCTCGACGAGGTCGCCGTTCCTAA
CGCTGTTGACGCTGATATCGACCGATTTAGAGACGTGATGATCGCTGATGGAACGGTGTTGCGGTTACACGAATTTCTCTCAGATCAGTTCGAAGCCCGC
CACGAGGAGCAGGCTGGAGCGAAGTCACCTGCCTCACAATGCCACAGAGCAGACGATTGAACAGCTCGATACTGCTAACGAGAAAACGCACGACAGCACC
TTGTTCAAAACAGGGCCGTGGCTTGAGAATCGCCTCATGCTGTTCGATCTCGCCCTACTTCAAGTACCGCCGGTTTGCGCTGATCGACGAAAATGACGGC
TACGTTCGTGAGTCGGCTGAAGCAGAACGCGAATCCGCTGATTACCGGAGAGCTACGAATCGCGGCCGCGCCATTCCCTTAGAGGGCAAGCAGCTCCGAG
CTGTTCTCGATGATCTTGACCGGAAATACATCGATGTGGAGGTCGAAGTCGAATTCCAAACGGGGGCCGTACAATGGGACACAGTCGCTGGATACGAAGC
GGTTTCGCGTCGTCGGCGTCCGCGACGAGGACGCCGACGACTACCACCTGTACATGACGAATTTAGCGAGGAAAGAGTTCTTTCCGGCGGATTTAGCGGA
GATCTACCGTGTCGGTGGGAAGTTGAGTTGCTGTTCCGGGAGCTGAAGACGCAGTACGAATTGGACGAGTTCGACACGAGTGACGAACACGTGGTGAGGA
TCTTATTGTACGCAGCGCTGCTGTCGCTGCTTGTAAGCCGCGACTCGTTGGATCTAGTCACTGAGCAGGCGGATGATGAGCGTTGTGTTTCCGACAGACC
TGCGACCGGTCGCACGCCCAGCTTATTCTCCTCGAACTCGGTGAGTTCCTTGGCTACTCACCACCGCCGCTTCTCGACCGGCTGATCGAAGACGCTCAAA
AGATCCACAAGCAACGACCAATCTTACAAGAGACGCTCGCTACCACTACACAACCGAGGTGTGAGGCTTAACTAAAGACGGATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 447 bp | 148 aa | 114 | 560 | + | No |
Chemistry : DDE
ORF sequence :
MRRLTTLFPSEFLEDHAEELGVVERDRKLQIPAFVWAFVFGFAQEQRTLAGFRRCYNSTADETISPGGFYQRLTPTLAEYLRDLVEHGLDEVAVPNAVDA
DIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKSPASQCHRADD
DIDRFRDVMIADGTVLRLHEFLSDQFEARHEEQAGAKSPASQCHRADD
Blast result :
Comments
ISH26 has been isolated through its inactivation of the bacteriorhodopsin (BR, bop) gene. There are 4 ISH26 copies on pHH1, and 4 copies on the chromosome of Halobacterium salinarum PHH1 (Ebert et al., 1986). ISH26 was described as harbouring two overlapping ORFs. Although the first ORF shares significant similarity with the putative transposases of other IS4 family members (26 % identity with IS231W, over a 143-aa overlap), the second ORF only shares very limited similarity, in the region of the conserved "E" residue. Detailed analyses suggest however that several frameshiftings could significantly increase this similarity. The first open reading frame is very closely related to the N terminal end of the Tpase of the different ISH8. Therefore, a reevaluation of ISH26 DNA sequence is needed to clarify this issue.
References
1] Pfeifer, F., and Betlach, M. (1985) Mol. Gen. Genet. 198, 449-455.
2] Ebert, K., and Goebel, W. (1985) Mol. Gen. Genet. 200, 96-102.
3] Ebert, K., Goebel, W., Moritz, A., Rdest, U., and Surek, B. (1986) System. Appl. Microbiol. 7, 30-35.
4] Pfeifer, F. (1986) System. Appl. Microbiol. 7, 36-40.
5] Ebert, K., Hanke, C., Delius, H., Goebel, W., and Pfeifer, F. (1987) Mol. Gen. Genet. 206, 81-87.
6] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
7] Pfeifer, F., Blaseio, U., and Horne, M. (1989) Can. J. Microbiol. 35, 96-100.
8] Pfeifer, F., and Englert, C. (1992) J. Bioenergetics and Biomembranes 24, 577-585.
9] Pfeifer, F., and Ghahraman, P. (1993) Mol. Gen. Genet. 238, 193-200.
2] Ebert, K., and Goebel, W. (1985) Mol. Gen. Genet. 200, 96-102.
3] Ebert, K., Goebel, W., Moritz, A., Rdest, U., and Surek, B. (1986) System. Appl. Microbiol. 7, 30-35.
4] Pfeifer, F. (1986) System. Appl. Microbiol. 7, 36-40.
5] Ebert, K., Hanke, C., Delius, H., Goebel, W., and Pfeifer, F. (1987) Mol. Gen. Genet. 206, 81-87.
6] DasSarma, S. (1989) Can. J. Microbiol. 35, 65-72.
7] Pfeifer, F., Blaseio, U., and Horne, M. (1989) Can. J. Microbiol. 35, 96-100.
8] Pfeifer, F., and Englert, C. (1992) J. Bioenergetics and Biomembranes 24, 577-585.
9] Pfeifer, F., and Ghahraman, P. (1993) Mol. Gen. Genet. 238, 193-200.