ISSen10
- Family IS3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP006057 | ND | Salmonella enterica | Salmonella enterica Salmonella enterica subsp. enterica Serovar Cubana CFSAN002050 plasmid |
DNA section
IS Length : 1336 bp
Ends
IR Length : 22/27
IRL : TGAACCGTACCGGGTTTCTTGGAGACTAAACTCTCAGTGAAAGGAGACCC
IRR : TGAACCGCCCCGGTTTTTTTGGAGAGTGTTTAGTCCGGAAGCTCAGGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAAGGAGAC | GTTTCATGGC | 0 |
DNA sequence
TGAACCGTACCGGGTTTCTTGGAGACTAAACTCTCAGTGAAAGGAGACCCAAAATGAAATCACAATCCGCCAAACGCTATCCCCCTGAACTGCGTGAGCG
CGCTGTCAGAATGTTGTTGGAACACCGTGATGAGTATGACTCTGAGCCGGATGCCATTCGTGCCATATCGTCAAAAATCGGGTGCCATTACGACACCCTA
CGAGCCTGGTTGCGTCAGCATAAAAATGATGTCCGTGGTGGTGTCAGCCCCGTCAGCACTGATGATGGTGGACTGAGCACCAATGAGCGCCAGCGTTTAA
AAGAACTGGAGCGTGAAAATCGGGAGCTACGCCGCAGCAATGATATATTGCGCCAGGCCTCAGCTTATTTTGCTCAGGCGGAGCTCGACCGCCACTGGAA
AAAATAATGCCACTTATGCAGCGTCTGAGTGGTACACACGGGGTCGGGCCGGTATGTCGCGAACTGGATATTGCCCCGTCGACATATTACTGGCATCAGC
AACGCCAGCTACATCCTGAGAAATGTAGCCAACGAGAAAAGTGCGATGCTCAAATCATCCAGGAGATAAAACGCGTTTACGGAGAAAATTACAGTGTCTA
TGGGGCACGAAAAGTCTGGCGTCAGTTGCTCCGCGAGGGCTTTAGCGTCGCCCGGTGTACTGTTGAACGCCTGATGAAAATAATAGGTCTTCGGGGCGTA
CTCCGTGGCAAAGTCATCAAGACCACGATCGGTCGTAAAACAGCGGCAGCAGCGGATCTGGTAAACCGCCAGTTTGTGGCAGAACGTCCCAATCAGCTTT
GGTGTGCAGATTTTACGTACGTTAGCACCTGGCATGGCTTCGCCTATGTGGCGTTCATCATCGATGTGTTCGCAGGCACGATCGTTGGCTGGCGGGTCTC
ATCGTCGATGGAAGCAAGGTTCGTGCTGGATGCTCTGGAACAAGCTCTGTGGGCACGTCGCCCCTCAGGTACAATCCATCACTCGGACAAGGGCTCCCAA
TACGTGTCACTGGCGTACACCCAGCGGCTGCAGGAGGCGGAGCTGTTGGCGTCGACAGGCAGTACAGGCGACTCTTACGATAATGCCCTGGCCGAGAGTA
TCAATGGCCTTTACAAAGCGGAAGTGATCCACCGTAAAAGCTGGAAAAACCACACGGAAGTGGAGCTTGCGACACTGGCGTGGGTGGATTGGTTCAACAA
TCGAAGGTTACTGGAACGGCTAGGACACATACCGCCGGTAGAAGCCGAAAAAGCTTATTATGCTACCAGTACAAATAATGATCTGGCAGCCTGAGCTTCC
GGACTAAACACTCTCCAAAAAAACCGGGGCGGTTCA
CGCTGTCAGAATGTTGTTGGAACACCGTGATGAGTATGACTCTGAGCCGGATGCCATTCGTGCCATATCGTCAAAAATCGGGTGCCATTACGACACCCTA
CGAGCCTGGTTGCGTCAGCATAAAAATGATGTCCGTGGTGGTGTCAGCCCCGTCAGCACTGATGATGGTGGACTGAGCACCAATGAGCGCCAGCGTTTAA
AAGAACTGGAGCGTGAAAATCGGGAGCTACGCCGCAGCAATGATATATTGCGCCAGGCCTCAGCTTATTTTGCTCAGGCGGAGCTCGACCGCCACTGGAA
AAAATAATGCCACTTATGCAGCGTCTGAGTGGTACACACGGGGTCGGGCCGGTATGTCGCGAACTGGATATTGCCCCGTCGACATATTACTGGCATCAGC
AACGCCAGCTACATCCTGAGAAATGTAGCCAACGAGAAAAGTGCGATGCTCAAATCATCCAGGAGATAAAACGCGTTTACGGAGAAAATTACAGTGTCTA
TGGGGCACGAAAAGTCTGGCGTCAGTTGCTCCGCGAGGGCTTTAGCGTCGCCCGGTGTACTGTTGAACGCCTGATGAAAATAATAGGTCTTCGGGGCGTA
CTCCGTGGCAAAGTCATCAAGACCACGATCGGTCGTAAAACAGCGGCAGCAGCGGATCTGGTAAACCGCCAGTTTGTGGCAGAACGTCCCAATCAGCTTT
GGTGTGCAGATTTTACGTACGTTAGCACCTGGCATGGCTTCGCCTATGTGGCGTTCATCATCGATGTGTTCGCAGGCACGATCGTTGGCTGGCGGGTCTC
ATCGTCGATGGAAGCAAGGTTCGTGCTGGATGCTCTGGAACAAGCTCTGTGGGCACGTCGCCCCTCAGGTACAATCCATCACTCGGACAAGGGCTCCCAA
TACGTGTCACTGGCGTACACCCAGCGGCTGCAGGAGGCGGAGCTGTTGGCGTCGACAGGCAGTACAGGCGACTCTTACGATAATGCCCTGGCCGAGAGTA
TCAATGGCCTTTACAAAGCGGAAGTGATCCACCGTAAAAGCTGGAAAAACCACACGGAAGTGGAGCTTGCGACACTGGCGTGGGTGGATTGGTTCAACAA
TCGAAGGTTACTGGAACGGCTAGGACACATACCGCCGGTAGAAGCCGAAAAAGCTTATTATGCTACCAGTACAAATAATGATCTGGCAGCCTGAGCTTCC
GGACTAAACACTCTCCAAAAAAACCGGGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 354 bp | 117 aa | 54 | 407 | + | No |
Description : First part of the transposase
ORF sequence :
MKSQSAKRYPPELRERAVRMLLEHRDEYDSEPDAIRAISSKIGCHYDTLRAWLRQHKNDVRGGVSPVSTDDGGLSTNERQRLKELERENRELRRSNDILR
QASAYFAQAELDRHWKK
QASAYFAQAELDRHWKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 344 | 1294 | + | No |
Description : Second part of the transposase
ORF sequence :
YIAPGLSLFCSGGARPPLEKIMPLMQRLSGTHGVGPVCRELDIAPSTYYWHQQRQLHPEKCSQREKCDAQIIQEIKRVYGENYSVYGARKVWRQLLREGF
SVARCTVERLMKIIGLRGVLRGKVIKTTIGRKTAAAADLVNRQFVAERPNQLWCADFTYVSTWHGFAYVAFIIDVFAGTIVGWRVSSSMEARFVLDALEQ
ALWARRPSGTIHHSDKGSQYVSLAYTQRLQEAELLASTGSTGDSYDNALAESINGLYKAEVIHRKSWKNHTEVELATLAWVDWFNNRRLLERLGHIPPVE
AEKAYYATSTNNDLAA
SVARCTVERLMKIIGLRGVLRGKVIKTTIGRKTAAAADLVNRQFVAERPNQLWCADFTYVSTWHGFAYVAFIIDVFAGTIVGWRVSSSMEARFVLDALEQ
ALWARRPSGTIHHSDKGSQYVSLAYTQRLQEAELLASTGSTGDSYDNALAESINGLYKAEVIHRKSWKNHTEVELATLAWVDWFNNRRLLERLGHIPPVE
AEKAYYATSTNNDLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1241 bp | 413 aa | 54 | 1294 | + | Yes |
Chemistry : DDE
ORF sequence :
MKSQSAKRYPPELRERAVRMLLEHRDEYDSEPDAIRAISSKIGCHYDTLRAWLRQHKNDVRGGVSPVSTDDGGLSTNERQRLKELERENRELRRSNDILR
QASAYFAQAELDRHWKKIMPLMQRLSGTHGVGPVCRELDIAPSTYYWHQQRQLHPEKCSQREKCDAQIIQEIKRVYGENYSVYGARKVWRQLLREGFSVA
RCTVERLMKIIGLRGVLRGKVIKTTIGRKTAAAADLVNRQFVAERPNQLWCADFTYVSTWHGFAYVAFIIDVFAGTIVGWRVSSSMEARFVLDALEQALW
ARRPSGTIHHSDKGSQYVSLAYTQRLQEAELLASTGSTGDSYDNALAESINGLYKAEVIHRKSWKNHTEVELATLAWVDWFNNRRLLERLGHIPPVEAEK
AYYATSTNNDLAA
QASAYFAQAELDRHWKKIMPLMQRLSGTHGVGPVCRELDIAPSTYYWHQQRQLHPEKCSQREKCDAQIIQEIKRVYGENYSVYGARKVWRQLLREGFSVA
RCTVERLMKIIGLRGVLRGKVIKTTIGRKTAAAADLVNRQFVAERPNQLWCADFTYVSTWHGFAYVAFIIDVFAGTIVGWRVSSSMEARFVLDALEQALW
ARRPSGTIHHSDKGSQYVSLAYTQRLQEAELLASTGSTGDSYDNALAESINGLYKAEVIHRKSWKNHTEVELATLAWVDWFNNRRLLERLGHIPPVEAEK
AYYATSTNNDLAA
Blast result :
Comments
ISSen10 is 85% (transposase) aa similar to ISEc25.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Hoffmann,M., Muruvanda,T., Pirone,C., Korlach,J., Timme,R., Payne,J., Evans,P., Meng,J., Brown,E.W. and Allard,M.W. (2014) Genome Announc 2 (5).
2] Hoffmann,M., Muruvanda,T., Pirone,C., Korlach,J., Timme,R., Payne,J., Evans,P., Meng,J., Brown,E.W. and Allard,M.W. (2014) Genome Announc 2 (5).