ISfinder Mirror Site

ISHahl1

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AP017372.2	ND	Halorhodospira halochloris	Halorhodospira halochloris Halorhodospira halochloris DSM 1059

DNA section

IS Length : 1813 bp

Ends

Left end : CAAAACGCGCCGCAGTGCTCCGCCTATAGCCGCGCAGCGGCTTAGGGGGTGGTGAAGCGGCGCATGGCGCGCAGCGCCATTACTCTGGGGCTTCCTGTTG II struct. : Yes
Right end : CGTGGAGGGAGGTCAGACCGGGTCATCCTTTGGATGAATCGGCACTCCCGTCGAAGCGCGAAACTCACGCCATAGCGTAAGCTTGGCGGGAGTAGTTCAT II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GGAGGGTAGGTGAGTC	CTAC	CGGGCACTGCCGGATGC	TCAT

DNA sequence

CAAAACGCGCCGCAGTGCTCCGCCTATAGCCGCGCAGCGGCTTAGGGGGTGGTGAAGCGGCGCATGGCGCGCAGCGCCATTACTCTGGGGCTTCCTGTTG
TTCGATGTACTGCTTCAAGACCGACAGCGGCGCACCACCGACGGTAATGATGCAGTAGCTGCGACTCCAGAATACCGGTTTGCGCCAGTAATACTGTTGA
AGATGCTCCCCGAACTCCTTGCGGATGAGCCGGGAGGTCACCGTCTTTAGATTGTTGACGAAGGCGGAGGGGGCGGTGCGCGGCGTCAACTCCAAGAGCA
GGTGCAGATGGTCGGGCTCGCCGTTGAACTCCAGCACCTCGCCTTCCCACTTTTCGGTCGTCGCCCGAGCGATTGCCTCTAAGCGGGAGAGCATCGGTTG
GGTGATGCACGGGCGTCGATACTTGGTAACCACAACCAAGTGGTATTGAAGTCGGAAGGTGCAGTGATATAGCTGCTTTAGTGGTTGCGATTCCATAACA
ACCAAGTATACTGGAAACATGATCCAACGCAAAGCCACCTATCGACTTTACCCAACAGCTGAGCAGCTTGCCGCCTTGGAGCATCAGCTGTGGGTTCACT
GCCTGCTGTGGAACGAGGCGCTCGCCGAGCGGCGCTGGGCTTGGAGCGAGCGGCAGGAGTCGTTGGGGTTCTCGGCACAGTGCAAGCGGCTGACCGAGTG
GCGTAGCCAATCGAAACTTCTGAGTGGACTCAATGCCCAGTCTGAGCAGGTCACCCTCAAACGGTTGGATCTGGCGTTTCAGCACTTCTTTCGCCGGGTG
AAGCAGGGCGAGACGCCGGGCTTCCCCCGCTTCAAGTCCCTGCACCGGTTCAAGGGCTGGGGGTACAAGACCCACGGCGACGGCTGGCGGCTGTTGACCA
ACGAGCAGATGCAGCACGGCACCCTGCGTCTCTCCGGCGTTGGTGCGGTCGCCGTTCGGGGCAGACCCCGCACCCCTGGCACACCCAAGACTTGCGAGAT
CATCAAACGCGGTGATCGCTGGTATGCCAGCATTACGCTGAACTGCGAGCCGGTGCGAGAGTGTGGCGAGAAGATGGGCGGGCTCGACTGGGGCTTGGAG
ACCTTCGCCACTGTGGCGACCGCGCAAGGTGCCGAGACCATCGATAACCCCCGCCACCTTGCCGGCACCTTGGATGCCATCCGTGGCGTACAGCGCGAGA
TCTCCCGCAAGGAGGAGGCGGCCAAGAAGGCGTCGGGTAGGAGTCGGGGCTTCCCCATCTCCAACCGGTTGCGCAAGGCGTACGATGAGTTGCGCCGGCT
GCATCGCAAGGTGGCCAATCAGCGCCACGACTTTCTCCATCAGGTCTCTGCCCGACTGATCAAAGAGTTCAGCGCTTTGGGCCTGGAGGCGCTGAGCATT
CGGAACATGACCGCCAAAGGCGGTCAGCATAAGAGGGGCTTGAACCGCGGTATTCTTGATGCCGCTGGCGGGGCCTTCCATCAATTGCTCGGGTACAAAG
CGGAAGAGGCTGGTGCTTGGGCGGTGGAGGCCCCGACGCGGCAGATCAAACCCTCGCAGACCTGCCACGCCTGCGGGCAGCAAGAGAAGAAACCGCTCGG
CCAGCGCTGGCACAGCTGCCCTTGCGGCGCGTCCTGTTCACGGGACGAGAACGCCGCTCGGGTGCTGTTGGCTTGGCTGGAGCGGAGTTTATCCGGTCGG
GAACCGGCCGATGCGTGGAGGGAGGTCAGACCGGGTCATCCTTTGGATGAATCGGCACTCCCGTCGAAGCGCGAAACTCACGCCATAGCGTAAGCTTGGC
GGGAGTAGTTCAT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
315 bp	104 aa	394	80	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MLSRLEAIARATTEKWEGEVLEFNGEPDHLHLLLELTPRTAPSAFVNNLKTVTSRLIRKEFGEHLQQYYWRKPVFWSRSYCIITVGGAPLSVLKQYIEQQ
EAPE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1275 bp	424 aa	519	1793	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MIQRKATYRLYPTAEQLAALEHQLWVHCLLWNEALAERRWAWSERQESLGFSAQCKRLTEWRSQSKLLSGLNAQSEQVTLKRLDLAFQHFFRRVKQGETP
GFPRFKSLHRFKGWGYKTHGDGWRLLTNEQMQHGTLRLSGVGAVAVRGRPRTPGTPKTCEIIKRGDRWYASITLNCEPVRECGEKMGGLDWGLETFATVA
TAQGAETIDNPRHLAGTLDAIRGVQREISRKEEAAKKASGRSRGFPISNRLRKAYDELRRLHRKVANQRHDFLHQVSARLIKEFSALGLEALSIRNMTAK
GGQHKRGLNRGILDAAGGAFHQLLGYKAEEAGAWAVEAPTRQIKPSQTCHACGQQEKKPLGQRWHSCPCGASCSRDENAARVLLAWLERSLSGREPADAW
REVRPGHPLDESALPSKRETHAIA

Blast result :Comments : 56% identity with ISCARN6 TnpB

Comments

ISHahl1 is 88% aa similar to ISCARN6.

References

1] Sarah Sonbol (2020) Direct submission.
2] Tsukatani,Y. and Hirose,Y. (2016) Direct GenBank submission.