ISPsp16

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003962 ND Pseudomonas sp.
Pseudomonas sp. VLB120 pSTY
DNA section
IS Length : 2574 bp

Ends


IR Length : 51/59

IRL : TGCGGATTCCACGCGCACTTGGACACCCATTCCACGAACACTTGGACACT
IRR : TGCGGATTCCACGACACTTGGACACTCAGCCCACGATCATTTGGACACTC

Insertion site


Left flankDirect repeatRight flankDR Length
TGGCCCTTGTCCTGGAGCAGGATTTTG0

DNA sequence

TGCGGATTCCACGCGCACTTGGACACCCATTCCACGAACACTTGGACACTGATTCCACGAGCATTTGGACAGTGATTTTCCACTGACCCAGCCCATGCTC
AGGCAGGCAGCGACGCAGGATTATTCACTACCATCGACCTCTTTTCTCGAAGCGAAGAGGTCGTCGTGGAGCGTATATCCATGCGTAAAATCCGAGAGGT
ACTACGCCTAAAGTTCGACGCCGGCCTGTCCGTGCGCAAGATCGCCACCAGCCTTCGCATCAGCAGTGGCAGCGCCGGCAACTACCTGCATCGCTTCAAC
GCTTGCGGGCTGACCTGGCCGACGGCGTTGTCGGATGCCGAACTGGAACGCTGCCTGTTCCCGCCAGCGCCGACGGTTCCCAGCGATCAACGTCCGATGC
CGGACTGGGCTCATGTCCATGCCGAACTGTGCCGTCCCGGCGTCACCCTGGCCCTGCTTTGGCAAGAGTATCGACTCAGCCATCCGCAAGGCTTCCAGTA
CAGCTGGTTCTGCGAGCATTACCGCCTCTGGGCCGCCAAGGTCGACGTGGTCATGCGCCAGGAACACCGTGCCGGCGAAAAGCTGTTTGTCGATTACGCT
GGCCAGACCGTGCCAGTCATTGATCGGCGAACCGGCGAGATCCGCCAAACGCAGATCTTCGTCGCCGTCCTCGGCGCGTCCAGCTACACCTTTGCCGAGG
CCACCTGGTCGCAGAAACTGCCCGACTGGCTGGGCTCGCACGTGCGCTGCTTCGCCTTTTTGGGCGGTACATCGCAGATCCTGGTACCGGACAATCTGCG
CAGCGGCGTTACCAAAGCGCATCGTTACGAGCCTGACATCAATCCCAGTTACCGCGACCTGGCCGAGCATTACGGCGTGGCCGTATTGCCTGCCAGATCC
CGCAAGCCTCGGGACAAAGCCAAGGTCGAAGTCGGCGTGCAAGTGGTCGAGCGCTGGATCCTGGCAGTGCTGCGCAATCGGCAGTTCTTTTCGCTGGGTG
AACTCAACACGGCCATCGGACTGCTGCTGGATCGCCTCAACCAAAAGCCCTTCAAAAAGCTGCCCGGCTCGCGCCGTTCGGCCTTCGAGGCCATCGACCA
ACCGGCGTTGCAACCGCTACCGGAACACCCTTACATCTACGCCGAGTGGAAAAAGGTGCGGGTGCACATCGACTACCACGTCGAGGTCGACGGTCACTTC
TACTCGGTGCCATACCAACTGGTGAAGCACCAACTCGAAGTGCGACTGACTGCGCAGACCGTCGAGTGCTTCCACGCCAACCAGCGCGTGGCCAGCCATC
TTCGCTCGCCGCACAAAGGCCGCCACACCACCCACACCGAGCACATGCCCAAGAGCCACCGCGAGCACGCCGAATGGACACCGCAACGGCTGATCCGCTG
GGCAGAGCAAACCGGGCCCAACACCGCTGGCGTCATCGCCTACATCCTCGAACGCCGAATCCACCCGCAGCATGGCTTCCGCGCCTGCCTGGGCATCCTG
CGCCTGAGCAAACAGCACGGCGAAGAGCGGCTGGAAGCCGCTTGCCAGCGTGCGCTGGCACTGGGCGCATGTAGCTACAAAAGCCTCGAATCGATCCTGC
GCCAAGGCTTGGAAAGGCTGCCGCTGACCCAGCAAAACCTGCCACTGCTGCCCGAAAAGCACATCAACCTGCGTGGCCCCGACTACTACCACTGACCTGA
AAAGGAGCATCAAGATGCTACCCAACCCGACTCTGGACAAGCTGCAAACCCTGCGGCTGCACGGCATGATCAAAGCGCTGAGCGAGCAACACGCAACGCC
CGACATCAGCGATCTTAGCTTCGACGAACGCCTTGGCCTGATGGTCGACCGCGAACTGACCGAGCGTGAAGATGCTCGGCTGACCACTCGCCTCAAATCC
GCGAGACTACGCCACAACGCCTGCTTGGAAGATATCGATTACCGCAGCCCACGCGGCCTGGACAAGGCCCTGATCCTGCAACTTGGTAGCGGCCAATGGC
TGCGTGATGGCCTGAACCTGATCATCGGCGGGCCGACAGGCGTAGGCAAAACCTGGCTCGCCTGCGCGCTGGCCCACAAGGCCTGCCGGGACGGTTACAG
CGTGCGTTATCTGCGTCTGCCTCGCTTGATGGAAGAACTGGGCCTGGCCCACGGCGACGGTCGCTTCGCCAAACTCATGGCGGGTTATGCCAAGACCGAT
CTGCTGATCCTGGACGACTGGGGCCTTGCACCGTTTACCGCCGCGCAGCGGCGCGACATGCTGGAACTGCTGGACGACCGTTACGGAAACCGCTCGACGC
TGGTGACCAGCCAGATGCCGGTGGATAAATGGCATGCGCTGATCGGTGATCCGACCTTGGGCGATGCGATCCTCGACCGGCTGGTGCACAACGCTTACCG
CATCGAACTGAAGGGCGAATCGATGCGCAGGCGCGCAACGAAATTGACTGCAACCGGGACTTCAGACTAACAATGCGAACCTGCGTCGCTGCGCTCCGAC
TGCCTGTCCAAGTGAGCGTGGAATGAGTGTCCAAATGATCGTGGGCTGAGTGTCCAAGTGTCGTGGAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa1811695+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIREVLRLKFDAGLSVRKIATSLRISSGSAGNYLHRFNACGLTWPTALSDAELERCLFPPAPTVPSDQRPMPDWAHVHAELCRPGVTLALLWQEYRLS
HPQGFQYSWFCEHYRLWAAKVDVVMRQEHRAGEKLFVDYAGQTVPVIDRRTGEIRQTQIFVAVLGASSYTFAEATWSQKLPDWLGSHVRCFAFLGGTSQI
LVPDNLRSGVTKAHRYEPDINPSYRDLAEHYGVAVLPARSRKPRDKAKVEVGVQVVERWILAVLRNRQFFSLGELNTAIGLLLDRLNQKPFKKLPGSRRS
AFEAIDQPALQPLPEHPYIYAEWKKVRVHIDYHVEVDGHFYSVPYQLVKHQLEVRLTAQTVECFHANQRVASHLRSPHKGRHTTHTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVIAYILERRIHPQHGFRACLGILRLSKQHGEERLEAACQRALALGACSYKSLESILRQGLERLPLTQQNLPLLPEKHINLRGP
DYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17152470+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPNPTLDKLQTLRLHGMIKALSEQHATPDISDLSFDERLGLMVDRELTEREDARLTTRLKSARLRHNACLEDIDYRSPRGLDKALILQLGSGQWLRDGL
NLIIGGPTGVGKTWLACALAHKACRDGYSVRYLRLPRLMEELGLAHGDGRFAKLMAGYAKTDLLILDDWGLAPFTAAQRRDMLELLDDRYGNRSTLVTSQ
MPVDKWHALIGDPTLGDAILDRLVHNAYRIELKGESMRRRATKLTATGTSD

 

Blast result :
Comments
ISPsp16 is 97% (Transposase) aa similar to ISPsy40.
References
1] Dongsheng Zhou (2020) Direct submission.
2] Volmer,J., Neumann,C., Buhler,B. and Schmid,A. (2014) Appl. Environ. Microbiol. 80 (20), 6539-6548.