ISLse3
- Family IS3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LR134394.1 | ND | Listeria seeligeri | Listeria seeligeri Listeria seeligeri plasmid pLIS4 Listeria seeligeri plasmid pLIS5 Listeria ivanovii subsp. londoniensis strain NCTC1270 |
DNA section
IS Length : 1265 bp
Ends
IR Length : 21/26
IRL : TGGATATATACTAATAAAGTAGACAGCATTTTTGATGGTATACTGTTTAT
IRR : TGGACAGATGTCAATAAAGTAGACAGAAAATAAGTTAATTTTTATAATGA
Comments : Ends defined by alignment of multiple copies of ISLse3
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tatttaattggatcaacatc | atttctttaacaatcaatca | 0 | |
| ctattttctctatgtctgtt | actgacatatttttaattct | 0 | |
| atttttttatcagaaatcat | ataataaaatgggtgtacgt | 0 | |
| aatgtattcttctatcgttc | ttatgagtattatcaatgtt | 0 | |
| tgcattcacttcaatatatc | ggttttccattatcttctta | 0 |
DNA sequence
TGGATATATACTAATAAAGTAGACAGCATTTTTGATGGTATACTGTTTATAAGTAGACACAAAAACGAAAGGAAGGCAGTTTATGTCCAATTACAAAAAA
TACGATGAAGATTTCAAAAAAAGCTTAGTTAATTTATATCATGGAGGAAAAACACAAACTTCGTTGTGTAAAGAATATGGTGTTTCCTTCACCGCTTTAT
CTCGTTGGGTAAAACAATATTCTGAAGTGAAAATTGAAGACGGTTCCATTCTTACAGCTAGACAAGTGAAAGAATTACAAAAGAAAAATGCTTTATTAGA
AGAAGAAAATTTAATTCTAAAAAAAGCCATTGCCATCTTCACGCCACACTCAAACAACGACTAAATGCTATCCACCTTTTGCGCTTTGAACATTCCATTG
TTCGTCTCTGCCATACTTTAAAGGTAAATAGAAGTACTTATTACAAACATTTCACTTTGCCACCTGCCCCTAGAACAGTGAAAAATCAACAAATTAAAAA
ACATATTTTATCCATTTATATGGAGTCTAAGAAAAGACTCGGTGCTGCGAAAGTAAAAGTTGTTCTTGAACGTGATTATGGTATTTCCATTAGCGTTGGA
CGAGTGTACCGACTAATGAAATCAATGAATCTGCCTAAAATGTCCACTACCAAGCCTAAATTTTCATATACGAAACCTAAGGTTTCTTTAGCTTGTCCGA
ATCATTTGAACCAACAGTTCAACCCAACTGAGCCCAATCGTGTTTGGACCAGTGATATTTCTTACATTCCTGTCAAAAATAGATTTGTCTATCTTTGTGT
TATTTTGGATTTATATTCTAGAAAAGTCATTGCTTGGAAAGTTAGGACTAGAATGACAACTTCACTGGTACTAGATACACTCCAAACAGCTTTAGATAAA
CGTCAACCTGCTCAGCCATTACTTTTCCATACAAATCGTGGAACTCAATATACATCCAGTGAAATGAGACAATTTTTAGATTATCATTCCCTCACCTATT
CTTACTCTAAAAAAGGATATCCGTGGGATAATGCAGTAACTGAATCTTTTTTCAAGTATATGAAAAAAGAAGAATTAGACCGTAGAGTCTTTAAAACGAT
TCAAGAAGTCACGCTTTCTTCTTTTGAATATATTGAAGGATTTTACAATCCCAAAAGACCTCATTCCGCTAATAATATGCTTACGCCGAATCAAAAAGAA
CGAAACTATTTTGAATCATTATAAAAATTAACTTATTTTCTGTCTACTTTATTGACATCTGTCCA
TACGATGAAGATTTCAAAAAAAGCTTAGTTAATTTATATCATGGAGGAAAAACACAAACTTCGTTGTGTAAAGAATATGGTGTTTCCTTCACCGCTTTAT
CTCGTTGGGTAAAACAATATTCTGAAGTGAAAATTGAAGACGGTTCCATTCTTACAGCTAGACAAGTGAAAGAATTACAAAAGAAAAATGCTTTATTAGA
AGAAGAAAATTTAATTCTAAAAAAAGCCATTGCCATCTTCACGCCACACTCAAACAACGACTAAATGCTATCCACCTTTTGCGCTTTGAACATTCCATTG
TTCGTCTCTGCCATACTTTAAAGGTAAATAGAAGTACTTATTACAAACATTTCACTTTGCCACCTGCCCCTAGAACAGTGAAAAATCAACAAATTAAAAA
ACATATTTTATCCATTTATATGGAGTCTAAGAAAAGACTCGGTGCTGCGAAAGTAAAAGTTGTTCTTGAACGTGATTATGGTATTTCCATTAGCGTTGGA
CGAGTGTACCGACTAATGAAATCAATGAATCTGCCTAAAATGTCCACTACCAAGCCTAAATTTTCATATACGAAACCTAAGGTTTCTTTAGCTTGTCCGA
ATCATTTGAACCAACAGTTCAACCCAACTGAGCCCAATCGTGTTTGGACCAGTGATATTTCTTACATTCCTGTCAAAAATAGATTTGTCTATCTTTGTGT
TATTTTGGATTTATATTCTAGAAAAGTCATTGCTTGGAAAGTTAGGACTAGAATGACAACTTCACTGGTACTAGATACACTCCAAACAGCTTTAGATAAA
CGTCAACCTGCTCAGCCATTACTTTTCCATACAAATCGTGGAACTCAATATACATCCAGTGAAATGAGACAATTTTTAGATTATCATTCCCTCACCTATT
CTTACTCTAAAAAAGGATATCCGTGGGATAATGCAGTAACTGAATCTTTTTTCAAGTATATGAAAAAAGAAGAATTAGACCGTAGAGTCTTTAAAACGAT
TCAAGAAGTCACGCTTTCTTCTTTTGAATATATTGAAGGATTTTACAATCCCAAAAGACCTCATTCCGCTAATAATATGCTTACGCCGAATCAAAAAGAA
CGAAACTATTTTGAATCATTATAAAAATTAACTTATTTTCTGTCTACTTTATTGACATCTGTCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 93 aa | 83 | 367 | + | No |
Description : First part of the transposase
ORF sequence :
MSNYKKYDEDFKKSLVNLYHGGKTQTSLCKEYGVSFTALSRWVKQYSEVKIEDGSILTARQVKELQKKNALLEEENLILKKAIAIFTPHSNND
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 320 aa | 262 | 1227 | + | No |
Description : Second part of the transposase
ORF sequence :
TSERITKEKCFIRRRKFNSKKSHCHLHATLKQRLNAIHLLRFEHSIVRLCHTLKVNRSTYYKHFTLPPAPRTVKNQQIKKHILSIYMESKKRLGAAKVKV
VLERDYGISISVGRVYRLMKSMNLPKMSTTKPKFSYTKPKVSLACPNHLNQQFNPTEPNRVWTSDISYIPVKNRFVYLCVILDLYSRKVIAWKVRTRMTT
SLVLDTLQTALDKRQPAQPLLFHTNRGTQYTSSEMRQFLDYHSLTYSYSKKGYPWDNAVTESFFKYMKKEELDRRVFKTIQEVTLSSFEYIEGFYNPKRP
HSANNMLTPNQKERNYFESL
VLERDYGISISVGRVYRLMKSMNLPKMSTTKPKFSYTKPKVSLACPNHLNQQFNPTEPNRVWTSDISYIPVKNRFVYLCVILDLYSRKVIAWKVRTRMTT
SLVLDTLQTALDKRQPAQPLLFHTNRGTQYTSSEMRQFLDYHSLTYSYSKKGYPWDNAVTESFFKYMKKEELDRRVFKTIQEVTLSSFEYIEGFYNPKRP
HSANNMLTPNQKERNYFESL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 380 aa | 83 | 1227 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNYKKYDEDFKKSLVNLYHGGKTQTSLCKEYGVSFTALSRWVKQYSEVKIEDGSILTARQVKELQKKNALLEEENLILKKSHCHLHATLKQRLNAIHLL
RFEHSIVRLCHTLKVNRSTYYKHFTLPPAPRTVKNQQIKKHILSIYMESKKRLGAAKVKVVLERDYGISISVGRVYRLMKSMNLPKMSTTKPKFSYTKPK
VSLACPNHLNQQFNPTEPNRVWTSDISYIPVKNRFVYLCVILDLYSRKVIAWKVRTRMTTSLVLDTLQTALDKRQPAQPLLFHTNRGTQYTSSEMRQFLD
YHSLTYSYSKKGYPWDNAVTESFFKYMKKEELDRRVFKTIQEVTLSSFEYIEGFYNPKRPHSANNMLTPNQKERNYFESL
RFEHSIVRLCHTLKVNRSTYYKHFTLPPAPRTVKNQQIKKHILSIYMESKKRLGAAKVKVVLERDYGISISVGRVYRLMKSMNLPKMSTTKPKFSYTKPK
VSLACPNHLNQQFNPTEPNRVWTSDISYIPVKNRFVYLCVILDLYSRKVIAWKVRTRMTTSLVLDTLQTALDKRQPAQPLLFHTNRGTQYTSSEMRQFLD
YHSLTYSYSKKGYPWDNAVTESFFKYMKKEELDRRVFKTIQEVTLSSFEYIEGFYNPKRPHSANNMLTPNQKERNYFESL
Blast result :Comments : The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Comments
ISLse3 is 79% (transposase) aa similar to ISLmo5.
ISLse3 was identified by in silico nucleotide sequence analysis of Listeria seeligeri and Listeria ivanovii plasmids.
ISLse3 was identified by in silico nucleotide sequence analysis of Listeria seeligeri and Listeria ivanovii plasmids.
References
1] Chmielowska C., Korsak D., Szuplewska M., Bartosik D. (2020) Direct submission.