ISLse2
- Family IS3
- Group IS3
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
ND | Listeria seeligeri | Listeria seeligeri Listeria seeligeri pLIS4 Listeria seeligeri pLIS5 Listeria ivanovii subsp. londoniensis WSLC 30151 |
DNA section
IS Length : 1247 bp
Ends
IR Length : 22/30
IRL : TGGGTGTAGAATTAAAATTGGACAAAAGATGATAGAATAAAACCTATCGA
IRR : TGATGTGATGTCAATAACTTGGACAAAAATAAATAGAACTTAATTCGCTT
Comments : Ends defined by alignment of multiple copies of ISLse1 and available empty insertion sites
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
atattccatttttggaagaa | GTT | aatgctatcgaatttaatgg | 3 |
atgtccaattacaaaaaaaa | CGA | tgaagatttcaaaaaaagct | 3 |
tatgatttttactaaacaaa | aagtgtgcagaaaatcatgt | 0 |
DNA sequence
TGGGTGTAGAATTAAAATTGGACAAAAGATGATAGAATAAAACCTATCGAAAACATCGGAGGAATTTGTTCAATGAGAAAGCTAGGGAAATGGGACACCA
CATTTAAAGAAACAATTGTAGAACTGTATATAGCTGGCACTTCCGTGCAAACTTTATCAGAAGATTATGGCATTGCAAAAGCAACCATCTATACATGGAT
TAAAAATAAGCAAACAAAAGAAGAAAAACAGAATCGTCCAGAGTTGGCGACAGAGCATGTGGAGATGCAAAAAGAAATCCAGCGATTGAAAGAGGAAAAT
GAGATTTTAAAAAAGTGTATCAGCATCTTCACGGAGAAAAAGAAAAACTAAGGGTTTCAGAAAAAGTGGCTTGGATTGAAAAACACCGAGAGATGCACGC
ACTAGGAACCATGTTACAAATGATTGAATTAGCGAAATCTACGTACTACGAATACAAAAGGAGAGCAATAAGCCAAAGAGAGCGGGCACGAAAAGTAAGG
GAGAAACGTGTCCAAGCGTTATTTTATGAAAATAAGCAGATTTATGGAGCTCGAAAGTTACAGTTTTTGTTACAAAAAGAAGGAATCCTGGTGACGGTTC
GAACGATTTCAGCAGATATGAAAAGACTACAACTACGTTGTTGTTATATTAAAAAATGGCAATCTAAGAAGGCTACGAAGCAAGAAGAACTCCTGACAAA
TGAAATAAAAACAAATCAACCGACAACCGCAGGAACGCATGTATTAACGGATATGACCTATGTATGGACGCAAAAAGATAAATGGGTGTATGTCATTACA
TTTTTAGACGCCTTTACCCGAAAGGTCCTTCATTTTGGTGTTGGAAAACAAATGGATAGCACCTTTGTAGATGGTCATACAAAGGCAGTTTTAGAAAAAT
ATCCCAGTATCACTTTACTGCATTCTGATCGTGGCAGCCAATATACCGCGGGTAGCTATCGCGATTTACTAAATGAAAATAACGTGATAGTTTCTTATAG
TAAGGCAGGTTATCCTTATGATAATGCAAAAATCGAATCTTACCACGCCAGTATTAAACGAGAACAATTATACCGGCTTGATTTTCAGCACATCAACGAT
GTGTATCAAGCTGTATTTAGCTATAATTATGGCTTTTATAATACAAAGCGTATTCATCAATCTTTAGGCTACTTAACACCAAATGATTTTGAAAGAAAAG
CGAATTAAGTTCTATTTATTTTTGTCCAAGTTATTGACATCACATCA
CATTTAAAGAAACAATTGTAGAACTGTATATAGCTGGCACTTCCGTGCAAACTTTATCAGAAGATTATGGCATTGCAAAAGCAACCATCTATACATGGAT
TAAAAATAAGCAAACAAAAGAAGAAAAACAGAATCGTCCAGAGTTGGCGACAGAGCATGTGGAGATGCAAAAAGAAATCCAGCGATTGAAAGAGGAAAAT
GAGATTTTAAAAAAGTGTATCAGCATCTTCACGGAGAAAAAGAAAAACTAAGGGTTTCAGAAAAAGTGGCTTGGATTGAAAAACACCGAGAGATGCACGC
ACTAGGAACCATGTTACAAATGATTGAATTAGCGAAATCTACGTACTACGAATACAAAAGGAGAGCAATAAGCCAAAGAGAGCGGGCACGAAAAGTAAGG
GAGAAACGTGTCCAAGCGTTATTTTATGAAAATAAGCAGATTTATGGAGCTCGAAAGTTACAGTTTTTGTTACAAAAAGAAGGAATCCTGGTGACGGTTC
GAACGATTTCAGCAGATATGAAAAGACTACAACTACGTTGTTGTTATATTAAAAAATGGCAATCTAAGAAGGCTACGAAGCAAGAAGAACTCCTGACAAA
TGAAATAAAAACAAATCAACCGACAACCGCAGGAACGCATGTATTAACGGATATGACCTATGTATGGACGCAAAAAGATAAATGGGTGTATGTCATTACA
TTTTTAGACGCCTTTACCCGAAAGGTCCTTCATTTTGGTGTTGGAAAACAAATGGATAGCACCTTTGTAGATGGTCATACAAAGGCAGTTTTAGAAAAAT
ATCCCAGTATCACTTTACTGCATTCTGATCGTGGCAGCCAATATACCGCGGGTAGCTATCGCGATTTACTAAATGAAAATAACGTGATAGTTTCTTATAG
TAAGGCAGGTTATCCTTATGATAATGCAAAAATCGAATCTTACCACGCCAGTATTAAACGAGAACAATTATACCGGCTTGATTTTCAGCACATCAACGAT
GTGTATCAAGCTGTATTTAGCTATAATTATGGCTTTTATAATACAAAGCGTATTCATCAATCTTTAGGCTACTTAACACCAAATGATTTTGAAAGAAAAG
CGAATTAAGTTCTATTTATTTTTGTCCAAGTTATTGACATCACATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
282 bp | 92 aa | 73 | 354 | + | No |
Description : First part of the transposase
ORF sequence :
MRKLGKWDTTFKETIVELYIAGTSVQTLSEDYGIAKATIYTWIKNKQTKEEKQNRPELATEHVEMQKEIQRLKEENEILKKCISIFTEKKKN
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
909 bp | 301 aa | 303 | 1211 | + | No |
Description : Second part of the transposase
ORF sequence :
DFKKVYQHLHGEKEKLRVSEKVAWIEKHREMHALGTMLQMIELAKSTYYEYKRRAISQRERARKVREKRVQALFYENKQIYGARKLQFLLQKEGILVTVR
TISADMKRLQLRCCYIKKWQSKKATKQEELLTNEIKTNQPTTAGTHVLTDMTYVWTQKDKWVYVITFLDAFTRKVLHFGVGKQMDSTFVDGHTKAVLEKY
PSITLLHSDRGSQYTAGSYRDLLNENNVIVSYSKAGYPYDNAKIESYHASIKREQLYRLDFQHINDVYQAVFSYNYGFYNTKRIHQSLGYLTPNDFERKA
N
TISADMKRLQLRCCYIKKWQSKKATKQEELLTNEIKTNQPTTAGTHVLTDMTYVWTQKDKWVYVITFLDAFTRKVLHFGVGKQMDSTFVDGHTKAVLEKY
PSITLLHSDRGSQYTAGSYRDLLNENNVIVSYSKAGYPYDNAKIESYHASIKREQLYRLDFQHINDVYQAVFSYNYGFYNTKRIHQSLGYLTPNDFERKA
N
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1139 bp | 378 aa | 73 | 1211 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKLGKWDTTFKETIVELYIAGTSVQTLSEDYGIAKATIYTWIKNKQTKEEKQNRPELATEHVEMQKEIQRLKEENEILKKVYQHLHGEKEKLRVSEKVA
WIEKHREMHALGTMLQMIELAKSTYYEYKRRAISQRERARKVREKRVQALFYENKQIYGARKLQFLLQKEGILVTVRTISADMKRLQLRCCYIKKWQSKK
ATKQEELLTNEIKTNQPTTAGTHVLTDMTYVWTQKDKWVYVITFLDAFTRKVLHFGVGKQMDSTFVDGHTKAVLEKYPSITLLHSDRGSQYTAGSYRDLL
NENNVIVSYSKAGYPYDNAKIESYHASIKREQLYRLDFQHINDVYQAVFSYNYGFYNTKRIHQSLGYLTPNDFERKAN
WIEKHREMHALGTMLQMIELAKSTYYEYKRRAISQRERARKVREKRVQALFYENKQIYGARKLQFLLQKEGILVTVRTISADMKRLQLRCCYIKKWQSKK
ATKQEELLTNEIKTNQPTTAGTHVLTDMTYVWTQKDKWVYVITFLDAFTRKVLHFGVGKQMDSTFVDGHTKAVLEKYPSITLLHSDRGSQYTAGSYRDLL
NENNVIVSYSKAGYPYDNAKIESYHASIKREQLYRLDFQHINDVYQAVFSYNYGFYNTKRIHQSLGYLTPNDFERKAN
Blast result :Comments : The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Comments
ISLse2 is 59% (transposase) aa similar to IS981.
ISLse2 was identified by in silico nucleotide sequence analysis of Listeria seeligeri plasmids. In plasmid pLIS4 two ISLse2 copies form a 27 kbp long composite transposon harboring a functional cadAC efflux pump conferring resistance to cadmium.
Nucleotide sequences of plasmids pLIS4 and pLIS5 will soon be available in GenBank database.
ISLse2 was identified by in silico nucleotide sequence analysis of Listeria seeligeri plasmids. In plasmid pLIS4 two ISLse2 copies form a 27 kbp long composite transposon harboring a functional cadAC efflux pump conferring resistance to cadmium.
Nucleotide sequences of plasmids pLIS4 and pLIS5 will soon be available in GenBank database.
References
1] Chmielowska C., Korsak D., Szuplewska M., Bartosik D. (2020) Direct submission.