ISfinder Mirror Site

ISPye31

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP020442	ND	Paracoccus yeei	Paracoccus yeei Paracoccus yeei FDAARGOS_252

DNA section

IS Length : 1052 bp

Ends

IR Length : 12/17

IRL : GAGCATCTTCGGTGAACGCAGAGTCGGTTTGAGGATTCCGTGGTTATTGG
IRR : GAGCAGATTCGACTTAATCCGGGTCATATCCAGCGGCCTCGAAATAGTGC

Comments : Empty insertion sites in other Paracoccus yeei strains enabled precisely defining ends.

Insertion site

Left flank	Direct repeat	Right flank	DR Length
agacaactgtcctcaccgcc	TA	ggtgacgagctcatcagctc	2

DNA sequence

GAGCATCTTCGGTGAACGCAGAGTCGGTTTGAGGATTCCGTGGTTATTGGTTGTCTGATTCACTGCTGCTGGTGATTTGCCAGGAGGTGAAGATGACGAG
GAGCTTGAGCGGAGACCTTCGGGGTCGGGTGATTGCCGCGATCGAGGACGGGATTTCAACGCGGGAGGCGGCGCGTCGCTTCCGGATCGGGATCGCGACG
GCGGGCGCCTGGTATCGGCGCTATCGCGAGACCGGCGAGACGGAGGCTCGCAAGCAGGGCCAGCCGTCACGTTCAAAGCTCGATGCGCACGAGGCTTTCG
TTCTCGGCTTGATCGAGGAGGCGCCGGATATCACTCTTGCCGAGATCGGGGAGCGCCTGGCCGCCGAGCGCGACGTGCAGGCTGCGCCTTCGACGGTGTG
GCTGTTTCTCGACCGACGCGGCATCACGTTCAAAAAAAGACGGCGCACGCCTCGGAGCAGCAGCGTCCAGATGTCCTGCGCCGCCGCATAGCCTGGTTCG
ACGGCCAACTCGACCTTGATCCCGAAAGACTGATCTTCATCGATGAGACCGCGGCATCCACAAAGATGGCCCGGCTGCGAGGGCGAGCGCCATGCGGCGA
GCGATGCCGGGCGGCCGTTCCCCACGGCCACTGGAAGACGACCACCTTCACCGCCGGCCTGCGGCTGTCCGGCATGGCGGCGCCGATGCTGCTCGGCGGT
CCGATGAACGGTCCGGCTTTCCTGGCATATGCCGAGCAGGTTCTCGCACCCGAGCTTCGCCCTGGCGACATCGTGGTCATGGACAATCTGCCCGCTCACA
AGATCAGCGGTGTGCGTGAGGCCATCGAGAAGGTCGGAGCGCGGCTCCTCTTCCTGCCGCCTTACTCGCCGGACTTCAACCCGATCGAGATGGCCTTCTC
GAAGCTCAAAGCCCTGCTCAGGAAGGCCGCCGCCAGAACCGTCGACGAACTCTGGTCCGTCGTCGCCGATTGCCTGTCAGCCTTCACACCCGAGGAGTGT
CGGCACTATTTCGAGGCCGCTGGATATGACCCGGATTAAGTCGAATCTGCTC

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
399 bp	132 aa	93	491	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTRSLSGDLRGRVIAAIEDGISTREAARRFRIGIATAGAWYRRYRETGETEARKQGQPSRSKLDAHEAFVLGLIEEAPDITLAEIGERLAAERDVQAAPS
TVWLFLDRRGITFKKRRRTPRSSSVQMSCAAA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
983 bp	326 aa	59	1041	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

FTAAGDLPGGEDDEELERRPSGSGDCRDRGRDFNAGGGASLPDRDRDGGRLVSALSRDRRDGGSQAGPAVTFKARCARGFRSRLDRGGAGYHSCRDRGAP
GRRARRAGCAFDGVAVSRPTRHHVQKKTAHASEQQRPDVLRRRIAWFDGQLDLDPERLIFIDETAASTKMARLRGRAPCGERCRAAVPHGHWKTTTFTAG
LRLSGMAAPMLLGGPMNGPAFLAYAEQVLAPELRPGDIVVMDNLPAHKISGVREAIEKVGARLLFLPPYSPDFNPIEMAFSKLKALLRKAAARTVDELWS
VVADCLSAFTPEECRHYFEAAGYDPD

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
949 bp	315 aa	93	1041	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRSLSGDLRGRVIAAIEDGISTREAARRFRIGIATAGAWYRRYRETGETEARKQGQPSRSKLDAHEAFVLGLIEEAPDITLAEIGERLAAERDVQAAPS
TVWLFLDRRGITFKKKTAHASEQQRPDVLRRRIAWFDGQLDLDPERLIFIDETAASTKMARLRGRAPCGERCRAAVPHGHWKTTTFTAGLRLSGMAAPML
LGGPMNGPAFLAYAEQVLAPELRPGDIVVMDNLPAHKISGVREAIEKVGARLLFLPPYSPDFNPIEMAFSKLKALLRKAAARTVDELWSVVADCLSAFTP
EECRHYFEAAGYDPD

Blast result :Comments : The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift

Comments

ISPye31 is 73% aa similar (transposase) to ISRm10-1.
ISPye31 was identified by in silico nucleotide sequence analysis of Paracoccus yeei FDAARGOS_252 genome.

References

1] Chmielowska C., Szuplewska M., Bartosik D. (2020) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.