ISfinder Mirror Site

ISPye28

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP020442.2	ND	Paracoccus yeei	Paracoccus yeei Paracoccus yeei FDAARGOS_252 Paracoccus yeei LM20

DNA section

IS Length : 1046 bp

Ends

IR Length : 18/29

IRL : CTGTTTGATCCCACATATTGATGGTGCGATCCTTTCTACGGAATGGAAAG
IRR : TTGTTCAGTCCCGGCATGTGGTGGCTCGGATTGACGATGAATCTGTCGGG

Comments : Depending on isoform, IRs are slightly different and range from 20/28 to 15/28. Empty insertion sites in other Paracoccus yeei strains enabled precisely defining ends and DRs.

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ggtcctgact	ctag	ggttttcggc	4
tcgtcatcaa	CTAG	agtcaggcgt	4
ggcggggccc	CTAG	gcatcaggcg	4
agtggttcgg	CTAG	caagccagcc	4

DNA sequence

CTGTTTGATCCCACATATTGATGGTGCGATCCTTTCTACGGAATGGAAAGAGGCCCTATGGGACAAGTTCGTCACGGGAGCGCCACGACTACGCACGCCG
TCCGAGCAGCACGACAGCGATCGCAAGCTTCGCTCGCGGTGTTGAGCCGCGAGTTTGGCATCAATCCGAAGACAGTGGCCAAGTGGCGCAAGCGGGCGAC
AGTCGAGGATCTGAAGACCGGACCGAAGGAGCCCCGCTCGACCGTTCTGAGCCAGGCGGAGGAGGCGATGGTGGTCGCGTTTCGGCGGCACACCCTGTTG
CCTCTGGACGATTGCCTCTACGCCCTGCAGCCGACGATCCCGCACCTGACCCGCTCGGCGCTGCACCGCTGCCTTCAGCGACATGGTATTTCCCGCCTGC
CCGATGTCGAGGGCGACAAGCCGAAGCGGCAGCGCTTCAAACGCTATCCCATCGGGTTCTTCCACATCGACATCGCCGAGGTCCAAACCGCTGAGGGCAA
GCTCTATCTCTTCGTGGCCATCGACCGCACCGGCAAGTTCGCCTTCACCCGGCTGGTCGAAAAGGCCGGCAAGATGGCTGCCGCCCAGTTTCTGCGCGAT
CTGGTCGCCGCTGTTCCCTACCGGCTGCATACCGTGCTGACCGACAACGGCGTCCAGTTCACCAATCACGCTCACCACACATACGCCTTCCACCACATCT
TTAATCGCGTCTGCGACGAGAACGGCATCGAGCACAGGCTCACCAGGATCAACCATCCCTGGACTAATGGTCAGGTCGAGCGGATGAACCGAACGATCAA
GGAGGGTGAAGCGGATCAGAAAACGATCCGGGGGATCGTTTTCCCGCTGAACATCAAACGCTTCCATTCCGACAGCCACGACCAGTTGCGCCGCCATCTC
GCCGACTTCCTCGCGGCCTACAACTTCGCCCGAAGGCTCAAGACCCTCAACGGCCGCACGCCCTACGAATACATCTGCAAGGTCTGGACTTCAGAACCCG
ACAGATTCATCGTCAATCCGAGCCACCACATGCCGGGACTGAACAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
989 bp	330 aa	58	1046	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGQVRHGSATTTHAVRAARQRSQASLAVLSREFGINPKTVAKWRKRATVEDLKTGPKEPRSTVLSQAEEAMVVAFRRHTLLPLDDCLYALQPTIPHLTRS
ALHRCLQRHGISRLPDVEGDKPKRQRFKRYPIGFFHIDIAEVQTAEGKLYLFVAIDRTGKFAFTRLVEKAGKMAAAQFLRDLVAAVPYRLHTVLTDNGVQ
FTNHAHHTYAFHHIFNRVCDENGIEHRLTRINHPWTNGQVERMNRTIKEGEADQKTIRGIVFPLNIKRFHSDSHDQLRRHLADFLAAYNFARRLKTLNGR
TPYEYICKVWTSEPDRFIVNPSHHMPGLNN

Blast result :Comments : The transposon uses the first stop codon after the end of the element, within the DR sequence. Compared to ISRel12 and ISNwi5 there seems to be a 14 amino acid long deletion within the transposase; however, isoforms of this IS, with the same 14 aa delet

Comments

Multiple isoforms (99-97% nucleotide sequence identity) in many different P. yeei strains.
ISPye28 is 80% aa similar to ISRel12.

References

1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.