ISKpn64

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP024529 ND Klebsiella pneumoniae
Klebsiella pneumoniae
Klebsiella pneumoniae strain INF157 plasmid INF157 p1
Klebsiella pneumoniae strain INF158 plasmid INF158 p1
Klebsiella pneumoniae strain KSB1_5D plasmid KSB1_5D p1
Klebsiella pneumoniae strain INF164 plasmid INF164 p1
Klebsiella pneumoniae strain INF163 plasmid INF163 p1
Klebsiella pneumoniae strain INF274 plasmid INF274 p1
Klebsiella pneumoniae strain INF278 plasmid INF278 p1
Klebsiella pneumoniae strain KSB1_9D plasmid KSB1_9D p1
DNA section
IS Length : 2396 bp

Ends


IR Length : 22/28

IRL : TGTTGATCAGCGCGCAAAAATGACCAGGTTGCCGGGGTTTTCAGCGTCCA
IRR : TGTCAATCAGCATCGAAAAATGACCAGGGATCAGCGTCCAAAATTGACCA

Insertion site


Left flankDirect repeatRight flankDR Length
TCAACAAACCGAGATGTGCCCATTCTGTATCGGGACT7

DNA sequence

TGTTGATCAGCGCGCAAAAATGACCAGGTTGCCGGGGTTTTCAGCGTCCAATTTTGACCACCCCGGAGTGTGTGCAACCATCCGGTTTTTGGACCGGAGA
ACCTGGGGTGTTAATCATGGAAACCGTGCTGAAAATACGGCGCATGCATCAGGTGGACGGCCAGTCCATCAGTGCCATCGCCAAAGTCACAGGCCTCTCT
CGCAATACCGTTCGCAAGTACCTTCGCCAACCTCCGTCCGAGCCGCCCAAGTACCGCCGCCAGCAGCCTGCCAAGCCTAAGTTGGGCGAGTTCGAGCCGG
CTCTCACCCAGTGGCTGGACCTCGATGCCCGGCGCCCCAAGCGCCAACGCCGCTGCGCCAAGCAGCTCTTCGAAGACCTGCAGCGCCTGGGCTACCAAGG
CGCCTACGACAGCGTGCAGCGCTTCGTCCGTGACCACAAAAGGCAGCCCGCCAAGACGGCAGCCTTTGTTCCCCTGACGTTCGCGGCCGGCGAGGCCTAC
CAGTTTGACTGGAGCGAGGAGGAGGTAGAGGTTGGTGGTGTGGTGCAACGGGTCAAAGCGGCCCACTTTCGTCTCACCCACAGCCGCCAGCCCTTTGTCA
TGGTGTTTCCCCGCGAGACCCAGGAGATGCTGTTTGCCGCCCATGATGCGGCCTTCCACTACTGGGGCGGCGTGCCGCGTCAGGGTATCTACGACAACCT
CAAGACCTGCGTCGACCAGGTGCTCGACGGCAAGGAGCGCCGCTTCAATCCCCGCTTCCTGGCCCTGATGAGCCATTATGTCATCGAGCCGGTGGCCTGC
ACCCCGGCCTCTGGCTGGGAGAAGGGCCAGGTCGAGAAACAGGTCCAGGATGTGCGCCGGCGCTGCTTCACCCCCAGACGCAAGGTAGACAGCCTGGCCG
AGCTCAACCGCCGGCTCATCCAGGAGTTGGAGCTGGCGGCACAGGCACGCCCGCATCCGGACGACCCGTCCCAAACGGTGTGGCAGCGCTTCGAGCTGGA
GCGGGCCGTCCTGCGCCCCCTGGCACCCCGCTTCAATGGCTATGTGGAGCGGGAGCTGCGCGTCGACAGCACCTGCCTCATCCACTTCGACCGCAACACC
TACAGTGTCGATAGCCGTTACGCCCATCGGCACGTGACCGCCAGGGTCTTCGCCGACCGACTCCAGGTGTACGCCCAGAGCGAGCTTATCGCCGACCATC
CTCGTCAATTTGGCCGGGACAAGACCCAGTACGACCCTTGGCACTACCTCGACCTGCTCGAGCACAAACCCGGCGCGCTGCGCCACGGCGCCCCCTTCAA
AGAGTGGACGCTGCCGCCGGCATTGAGCCGCTTGCAGCAGCAACTGATGGCTCGCAAGGGCGGTGATCGGGAGATGGTCAAGCTGCTGCTGGCCGCTAGG
CAGGATGGACTGGAGCTGCTGGAGCAGGCCTGCCGGCAGGTACTCCAACTGGGCGGAAGTTCCGCCGAGCTGGTGCTCAACCAACTGCAGCGGCTGCGCC
GCCCCGCCGAGGCGCCGGTGATCCATGCCCAGGTGGTGCCACTGAGCCGGCCGCCAGAGGCCAACTGCCAGCGCTATGACCGCCTGTTGCCGGGAGGTCA
GCATGTCAGCCGATGAGATCTACCAACGCCTGCAAGGGCTGAAACTGTTTGGCATGGCTGCTTTGTGCGACGAGCTGCTGGGGCATAGGGAGCGGCCCCT
GCCGCCGCCGGAGCAGTGGCTCAGACGGATGATCGAAGCCGAAGAGTCCGAGCGCCAATGCCGCTCGATCCGCTACCAGATGGGCATCGCCAAGTTCCCG
CTGGCCCGGGATCTGGACAGCTTCGACACCCGCGACTCACCGGTCAGCCCCGAGCAGCTGCGTGCGCTGGATCGGGATGACTTCCTAGCGCAGAAGCGCA
ACCTATTGCTGATCGGCGGGACCGGCACGGGGAAAACTCACCTGGCCATCGCCTTGGGGCGCAACGCCGTGCGCCGGGGCAAGCGGGTGCGCTTCTACGG
CGTGGTGGATCTGGTCAACCAACTGGAGCAGGAGAAGGCGGCCGGCAAAGCCGGACAACTGGCCAACCGGCTGGTCAATGTGGATGCCGTGATCCTGGAC
GAGCTGGGCTATCTGCCGTTTGCCGAAAGCGGTGGCGCCCTGCTGTTTCATCTCATCAGCAAGCTGTACGAGCGCACCAGCCTGATCCTGACCACCAACC
TGACCTTCAGCGAATGGGGCAAGGTGTTTACGGACGAGAAGATGACCACGGCGATGCTGGATCGGATCACTCATCACTGCGAGATCATCGAGACCGGAAA
CGACTCCTACCGCTTCAAACAGCGGATGAAATCAGCCAGCTAACCCTGGTCAATTTTGGACGCTGATCCCTGGTCATTTTTCGATGCTGATTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1500 bp499 aa1171616+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

METVLKIRRMHQVDGQSISAIAKVTGLSRNTVRKYLRQPPSEPPKYRRQQPAKPKLGEFEPALTQWLDLDARRPKRQRRCAKQLFEDLQRLGYQGAYDSV
QRFVRDHKRQPAKTAAFVPLTFAAGEAYQFDWSEEEVEVGGVVQRVKAAHFRLTHSRQPFVMVFPRETQEMLFAAHDAAFHYWGGVPRQGIYDNLKTCVD
QVLDGKERRFNPRFLALMSHYVIEPVACTPASGWEKGQVEKQVQDVRRRCFTPRRKVDSLAELNRRLIQELELAAQARPHPDDPSQTVWQRFELERAVLR
PLAPRFNGYVERELRVDSTCLIHFDRNTYSVDSRYAHRHVTARVFADRLQVYAQSELIADHPRQFGRDKTQYDPWHYLDLLEHKPGALRHGAPFKEWTLP
PALSRLQQQLMARKGGDREMVKLLLAARQDGLELLEQACRQVLQLGGSSAELVLNQLQRLRRPAEAPVIHAQVVPLSRPPEANCQRYDRLLPGGQHVSR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
768 bp255 aa15762343+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTACCREVSMSADEIYQRLQGLKLFGMAALCDELLGHRERPLPPPEQWLRRMIEAEESERQCRSIRYQMGIAKFPLARDLDSFDTRDSPVSPEQLRALDR
DDFLAQKRNLLLIGGTGTGKTHLAIALGRNAVRRGKRVRFYGVVDLVNQLEQEKAAGKAGQLANRLVNVDAVILDELGYLPFAESGGALLFHLISKLYER
TSLILTTNLTFSEWGKVFTDEKMTTAMLDRITHHCEIIETGNDSYRFKQRMKSAS

 

Blast result :
Comments
ISKpn64 is 66% aa similar (transposase) to ISMdi27.
References
1] Ambrose SJ. and Hall RM. (2020) Direct submission