ISH2
- Family IS4
- Group ISH8
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
J01726 | ND | Halobacterium salinarum | Halobacterium tunesiensis A2 Halobacterium salinarum R1 Halobacterium salinarum PHH1 plasmid pHH1 Halobacterium salinarum NRC-1 plasmid pNRC100 Halobacterium salinarum NRC34020 Halobacterium salinarum NRL Halobacterium salinarum S9 |
DNA section
IS Length : 521 bp
Ends
IR Length : 19
IRL : CATTCGTCTTTAGTTAAGAAATCGCGTGACAGCGGTAGGATCTCTTCGCT
IRR : CATTCGTCTTTAGTTAAGAGCGAAACCGCCACATGGCGTAGGGCTGAACA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
ATCCGCGTGT | CGGCTCCGTG | TCTGACGGTT | 10 |
TGCTGGGGTA | TGGCCTCACA | ATGGTACNNN | 10 |
ATCACGACTA | CTGGGGAAGC | TTGGTTATCC | 10 |
NNNNNNCTTT | CTCGTTCGCT | ACGGNNNNNN | 10 |
GTCGTAGATG | ATACCCGGAT | CTTCGAGCTG | 10 |
NNNNNNTATA | GTTAACGCCA | ACATNNNNNN | 10 |
NNNNNNNNNN | TCCAGGGCGT | NNNNNNNNNN | 10 |
NNNNNNNNNN | TGCCTCCGAG | NNNNNNNNNN | 10 |
NNNNNNNNNN | GTGCGAACGG | NNNNNNNNNN | 10 |
NNNNNNNNNN | ACGGGAAGAC | NNNNNNNNNN | 10 |
ACTACGACCA | AGGCGGCTAC | TATCACGTCT | 10 |
CGATGCCTAA | CTCGCGGAGG | TCATCGAAAA | 10 |
CCGAGTACAA | CCCAGAGGAC | TTCCCTGGGG | 10 |
CTTCGATATA | GTTAACGCCA | ACATCTGGTC | 10 |
TTCTGGAAAG | CATCATCCTC | TTCTTGTGCC | 10 |
GATTCCGGAA | CCACGTACGT | TTCCGTACCA | 10 |
TTGGATGTTC | CAGGGGCAAT | AGCACCCCAA | 10 |
NNNNNNNNNN | GAATACACACG | NNNNNNNNNN | 11 |
NNNNNNNNNN | ACCCAACAGGT | NNNNNNNNNN | 11 |
NNNNNNNNNN | ACCCAGCCCCG | NNNNNNNNNN | 11 |
TATGGAAGAA | CCGTTGAAAAG | TATGTTCGCA | 11 |
NNNNNNNNNN | GTCCGGGTACAG | NNNNNNNNN | 12 |
NNNNNNCAGA | ACCCCATCTACTGGGCGCGG | TACGNNNNNN | 20 |
CTCGAAGATA | CGTCGCATAATCAGTGTAAAAGTCAATCAGTTCTGTTCCGCCATAGGGGGAGAGT | GTTGGGACTC | 55 |
TGCCGACACG | CGCGGAACGA | 0 | |
ACCTCCTCCG | CCTCCACAGG | 0 |
DNA sequence
CATTCGTCTTTAGTTAAGAAATCGCGTGACAGCGGTAGGATCTCTTCGCTGTGCAAGACGCGGCTGAGATCTCTTTTGATAATATTTTAACCTAAATCGA
AATAAGACGATAATCTTACCGTGCACCCGGTGCACGTATTTCTAAGAGCGTCTAAGACTATGGCTCGAACCAAAATGGGCGTCTCCATCCGAACTGAACT
CGTTGATGAACTCGATTCACTCGTCGATGAGTGTTCAGATCTCGGAGCAAGCCGCTCCGAGATCGTTGAAGCCATCCTCACAGCATATTTTCAGAACGAT
GAAGACCAAATCAAACAGACGCGAGAGCTGATTATCCGCAACAGAAAACGCTCTAACTCGTAGAGAAGTTCGTGCACCGAGTGCACGAACTTCTTAGAGC
GTCATTCAAAACCACGACCTAGCTAAATTAATATCGGATTGGTCTTGGGCGAATAGAAATCTTCTCTTCACTGTTCAGCCCTACGCCATGTGGCGGTTTC
GCTCTTAACTAAAGACGAATG
AATAAGACGATAATCTTACCGTGCACCCGGTGCACGTATTTCTAAGAGCGTCTAAGACTATGGCTCGAACCAAAATGGGCGTCTCCATCCGAACTGAACT
CGTTGATGAACTCGATTCACTCGTCGATGAGTGTTCAGATCTCGGAGCAAGCCGCTCCGAGATCGTTGAAGCCATCCTCACAGCATATTTTCAGAACGAT
GAAGACCAAATCAAACAGACGCGAGAGCTGATTATCCGCAACAGAAAACGCTCTAACTCGTAGAGAAGTTCGTGCACCGAGTGCACGAACTTCTTAGAGC
GTCATTCAAAACCACGACCTAGCTAAATTAATATCGGATTGGTCTTGGGCGAATAGAAATCTTCTCTTCACTGTTCAGCCCTACGCCATGTGGCGGTTTC
GCTCTTAACTAAAGACGAATG
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
204 bp | 67 aa | 160 | 363 | + | No |
Chemistry : DDE
ORF sequence :
Blast result :
Comments
ISH2 is associated with the inactivation of the bacteriorhodopsin (bop) gene (coding for the purple membrane protein), and the appearance of Vac- (gas vacuole formation) and Rub- (bacterioruberin) phenotypes.
In the Pum- (purple membrane protein) mutants, the bacterio-opsin gene has been inactivated by the insertion of one of two transposable elements (ISH1 or ISH2) (DasSarma et al., 1983).
ISH2 shares nearly perfect terminal homology (but no internal homology) with ISH26, and it is possible that ISH2 transposition is complemented in trans by ISH26 (Ebert et al., 1987). An "A" should be added at position 388 (Pfeifer and Blaseio, 1989).
All coordinates have been changed accordingly. ISH2 is present in multiple copies in the Halobacterium salinarum S9 (formerly Halobacterium halobium) genome.
Four copies are present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 70690-71210 ; 74654-75174 ; 150254-150774 ; 153539-154059.
The ISH2 protein was identified upon analysis of "The low molecular weight proteome of Halobacterium salinarum."
In the Pum- (purple membrane protein) mutants, the bacterio-opsin gene has been inactivated by the insertion of one of two transposable elements (ISH1 or ISH2) (DasSarma et al., 1983).
ISH2 shares nearly perfect terminal homology (but no internal homology) with ISH26, and it is possible that ISH2 transposition is complemented in trans by ISH26 (Ebert et al., 1987). An "A" should be added at position 388 (Pfeifer and Blaseio, 1989).
All coordinates have been changed accordingly. ISH2 is present in multiple copies in the Halobacterium salinarum S9 (formerly Halobacterium halobium) genome.
Four copies are present in pNRC100 of Halobacterium sp. NRC-1 at coordinates 70690-71210 ; 74654-75174 ; 150254-150774 ; 153539-154059.
The ISH2 protein was identified upon analysis of "The low molecular weight proteome of Halobacterium salinarum."
References
1] DasSarma S., RajBhandary U.L., and Khorana H.G. (1983) Proc. Natl. Acad. Sci., USA 80, 2201-2205.
2] Pfeifer F., Betlach M., Martienssen R., Friedman J., and Boyer H.W. (1983) Mol. Gen. Genet. 191, 182-188.
3] DasSarma S., RajBhandary U.L., and Khorana H.G. (1984) Proc. Natl. Acad. Sci., USA 81, 125-129.
4] Pfeifer F., Friedman J., Boyer H.W., and Betlach M. (1984) Nucl. Acids Res. 12, 2489-2497.
5] Pfeifer F., and Betlach M. (1985) Mol. Gen. Genet. 198, 449-455.
6] Ebert K., Goebel W., Moritz A., Rdest U., and Surek B. (1986) System. Appl. Microbiol. 7, 30-35.
7] Pfeifer F. (1986) System. Appl. Microbiol. 7, 36-40.
8] Ebert K., Hanke C., Delius H., Goebel W., and Pfeifer F. (1987) Mol. Gen. Genet. 206, 81-87.
9] Pfeifer F., Blaseio U., and Ghahraman P. (1988) J. Bacteriol. 170, 3718-3724.
10] DasSarma S. (1989) Can. J. Microbiol. 35, 65-72.
11] Pfeifer F., and Blaseio U. (1989) J. Bacteriol. 171, 5135-5140.
12] Pfeifer F., Blaseio U., and Horne M. (1989) Can. J. Microbiol. 35, 96-100.
13] Ng, W.-L., Kothakota, S., and DasSarma, S. (1991) J. Bacteriol. 173, 1958-1964.
14] Pfeifer F., and Englert C. (1992) J. Bioenergetics and Biomembranes 24, 577-585
15] Wailap V. Ng., Stacy A. Ciufo, Smith T.M., Bumgarner R.E., Baskin D., Faust J., Hall B., Loretz C., Seto J., Slagel J., Hood L., and DasSarma S. (1998) Genome Research 8, 1131-1141
16] Klein C., Aivaliotis M., Olsen J.V., Falb M., Besir H., Scheffer B., Bisle B., Tebbe A., Konstantinidis K., Siedler F., Pfeiffer F., Mann M., Oesterhelt D. (2007) J. Proteome Res. 6: 1510-1518
17] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91, 335-346
2] Pfeifer F., Betlach M., Martienssen R., Friedman J., and Boyer H.W. (1983) Mol. Gen. Genet. 191, 182-188.
3] DasSarma S., RajBhandary U.L., and Khorana H.G. (1984) Proc. Natl. Acad. Sci., USA 81, 125-129.
4] Pfeifer F., Friedman J., Boyer H.W., and Betlach M. (1984) Nucl. Acids Res. 12, 2489-2497.
5] Pfeifer F., and Betlach M. (1985) Mol. Gen. Genet. 198, 449-455.
6] Ebert K., Goebel W., Moritz A., Rdest U., and Surek B. (1986) System. Appl. Microbiol. 7, 30-35.
7] Pfeifer F. (1986) System. Appl. Microbiol. 7, 36-40.
8] Ebert K., Hanke C., Delius H., Goebel W., and Pfeifer F. (1987) Mol. Gen. Genet. 206, 81-87.
9] Pfeifer F., Blaseio U., and Ghahraman P. (1988) J. Bacteriol. 170, 3718-3724.
10] DasSarma S. (1989) Can. J. Microbiol. 35, 65-72.
11] Pfeifer F., and Blaseio U. (1989) J. Bacteriol. 171, 5135-5140.
12] Pfeifer F., Blaseio U., and Horne M. (1989) Can. J. Microbiol. 35, 96-100.
13] Ng, W.-L., Kothakota, S., and DasSarma, S. (1991) J. Bacteriol. 173, 1958-1964.
14] Pfeifer F., and Englert C. (1992) J. Bioenergetics and Biomembranes 24, 577-585
15] Wailap V. Ng., Stacy A. Ciufo, Smith T.M., Bumgarner R.E., Baskin D., Faust J., Hall B., Loretz C., Seto J., Slagel J., Hood L., and DasSarma S. (1998) Genome Research 8, 1131-1141
16] Klein C., Aivaliotis M., Olsen J.V., Falb M., Besir H., Scheffer B., Bisle B., Tebbe A., Konstantinidis K., Siedler F., Pfeiffer F., Mann M., Oesterhelt D. (2007) J. Proteome Res. 6: 1510-1518
17] Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp.A., Soppa J., Tittor J., Oesterhelt D. (2008) Genomics 91, 335-346