ISFop4
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP022377 | ND | Francisella opportunistica | Francisella sp. MA067296 Francisella opportunistica Francisella opportunistica PA05-1188 Francisella sp. PA05-1188 |
DNA section
IS Length : 1266 bp
Ends
IR Length : 22/26
IRL : TATAGTGGTACGGTTTTTTCGGACAGGTAGCTAAGAAGGTAAAATAGGTG
IRR : TATAGTGGTGAATTTTTTCGGACAGTATTTAGTCAAAAAATAGATGTCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGATGATAA | tcc | TAAGCAAAAA | 3 |
| TTTTAAATAG | cac | ACCCCTCTTC | 3 |
DNA sequence
TATAGTGGTACGGTTTTTTCGGACAGGTAGCTAAGAAGGTAAAATAGGTGTCTTCGATAAGAAAAGGAGACATAAATGAAGAAGAAAAAATCTCATAGTG
CTGCATTTAAATATGAGGTATCAGTTGTAGCCATAAAAGGTGAATTAACCCAAGCTCAAATCAGTAGTAAATACGAAATTCACTCTAGCCAGATTACAAG
CTGGAAGAAGCAGGCTTTAGAGAGCATAAAACAACTATTTTCAGGTAAACTTCCGCCAGTTAAGCAAGATTTATTAGACGAAAAAGAAAAATCAAAGCTA
TACGAAGAAATTGGTAGGCTCAAAGTGGAGCTTGACTGGATGAAAAAAAAATCTAATTTTGAGTTGTAGGGATAAGAAAATGTTAATAGAACCAAATAAT
CCAGATATCCCAATAGTCAGACAGTGTCAACTTTTGGGCTTAAATAGATCAGTATATTATTATCAGTATAAAGGTATAGATGAGCATACTGAAAACTTAA
TGCGTCTAATAGATGAGCGTTATACAGCAAGGCCACATGAAAGCTCTAGAAAGATTATGGTATACCTAAATAACCTTGGATACGATGTTACTCGAAATCA
AGTAAAATACTTGATGGATAAAATGGGTTTACAAGCTATATACCCAAAGCCAATCACAAGCGCAAAATCTAGACAAGAACATATTATATATCCATATCTA
CTAAATGATATTTGTGTTTACTACCCTAACCAAGTATGGTGCTCAGATATAACATATATTCGTATGAAACATGGGCATATATATCTTGTAGCAATAATGG
ACTGGTATAGTAGGTATGTAATTGATTGGCAGTTGAGCATTAGTTTAGAAGCAGAATTTTGTATTGATACTCTTAAGAGAGCTTTAGCTAGTAGTAGCTG
TGGTATTTTCAATACAGATCAAGGAGCACAGTATACTTCAAACGCTTGGATAAATACCTTGCTAAATAAAGGTATCTCAATTAGTATGGATGCTAAAGGT
AGATGTTTTGATAATATATTTATTGAGCGTCTATGGAGAAGTGTTAAATACGAATATATATACCTAAAAGAATTTGAGTCAGTAGCTGAAGTTAGAGAAG
CTCTGACTGATTACTTTGAATATTATAATACTGATAGATATCATCAAAGTTTAGAATATAAGACTCCTGCTGATGTGTATTTTAGTAAAGTTAAAATAGA
AAATATTTTTGATAATTTGACATCTATTTTTTGACTAAATACTGTCCGAAAAAATTCACCACTATA
CTGCATTTAAATATGAGGTATCAGTTGTAGCCATAAAAGGTGAATTAACCCAAGCTCAAATCAGTAGTAAATACGAAATTCACTCTAGCCAGATTACAAG
CTGGAAGAAGCAGGCTTTAGAGAGCATAAAACAACTATTTTCAGGTAAACTTCCGCCAGTTAAGCAAGATTTATTAGACGAAAAAGAAAAATCAAAGCTA
TACGAAGAAATTGGTAGGCTCAAAGTGGAGCTTGACTGGATGAAAAAAAAATCTAATTTTGAGTTGTAGGGATAAGAAAATGTTAATAGAACCAAATAAT
CCAGATATCCCAATAGTCAGACAGTGTCAACTTTTGGGCTTAAATAGATCAGTATATTATTATCAGTATAAAGGTATAGATGAGCATACTGAAAACTTAA
TGCGTCTAATAGATGAGCGTTATACAGCAAGGCCACATGAAAGCTCTAGAAAGATTATGGTATACCTAAATAACCTTGGATACGATGTTACTCGAAATCA
AGTAAAATACTTGATGGATAAAATGGGTTTACAAGCTATATACCCAAAGCCAATCACAAGCGCAAAATCTAGACAAGAACATATTATATATCCATATCTA
CTAAATGATATTTGTGTTTACTACCCTAACCAAGTATGGTGCTCAGATATAACATATATTCGTATGAAACATGGGCATATATATCTTGTAGCAATAATGG
ACTGGTATAGTAGGTATGTAATTGATTGGCAGTTGAGCATTAGTTTAGAAGCAGAATTTTGTATTGATACTCTTAAGAGAGCTTTAGCTAGTAGTAGCTG
TGGTATTTTCAATACAGATCAAGGAGCACAGTATACTTCAAACGCTTGGATAAATACCTTGCTAAATAAAGGTATCTCAATTAGTATGGATGCTAAAGGT
AGATGTTTTGATAATATATTTATTGAGCGTCTATGGAGAAGTGTTAAATACGAATATATATACCTAAAAGAATTTGAGTCAGTAGCTGAAGTTAGAGAAG
CTCTGACTGATTACTTTGAATATTATAATACTGATAGATATCATCAAAGTTTAGAATATAAGACTCCTGCTGATGTGTATTTTAGTAAAGTTAAAATAGA
AAATATTTTTGATAATTTGACATCTATTTTTTGACTAAATACTGTCCGAAAAAATTCACCACTATA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 76 | 369 | + | No |
Description : First part of the transposase
ORF sequence :
MKKKKSHSAAFKYEVSVVAIKGELTQAQISSKYEIHSSQITSWKKQALESIKQLFSGKLPPVKQDLLDEKEKSKLYEEIGRLKVELDWMKKKSNFEL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 344 | 1234 | + | No |
Description : Second part of the transposase
ORF sequence :
KKNLILSCRDKKMLIEPNNPDIPIVRQCQLLGLNRSVYYYQYKGIDEHTENLMRLIDERYTARPHESSRKIMVYLNNLGYDVTRNQVKYLMDKMGLQAIY
PKPITSAKSRQEHIIYPYLLNDICVYYPNQVWCSDITYIRMKHGHIYLVAIMDWYSRYVIDWQLSISLEAEFCIDTLKRALASSSCGIFNTDQGAQYTSN
AWINTLLNKGISISMDAKGRCFDNIFIERLWRSVKYEYIYLKEFESVAEVREALTDYFEYYNTDRYHQSLEYKTPADVYFSKVKIENIFDNLTSIF
PKPITSAKSRQEHIIYPYLLNDICVYYPNQVWCSDITYIRMKHGHIYLVAIMDWYSRYVIDWQLSISLEAEFCIDTLKRALASSSCGIFNTDQGAQYTSN
AWINTLLNKGISISMDAKGRCFDNIFIERLWRSVKYEYIYLKEFESVAEVREALTDYFEYYNTDRYHQSLEYKTPADVYFSKVKIENIFDNLTSIF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1159 bp | 386 aa | 76 | 1234 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKKKSHSAAFKYEVSVVAIKGELTQAQISSKYEIHSSQITSWKKQALESIKQLFSGKLPPVKQDLLDEKEKSKLYEEIGRLKVELDWMKKKNLILSCRD
KKMLIEPNNPDIPIVRQCQLLGLNRSVYYYQYKGIDEHTENLMRLIDERYTARPHESSRKIMVYLNNLGYDVTRNQVKYLMDKMGLQAIYPKPITSAKSR
QEHIIYPYLLNDICVYYPNQVWCSDITYIRMKHGHIYLVAIMDWYSRYVIDWQLSISLEAEFCIDTLKRALASSSCGIFNTDQGAQYTSNAWINTLLNKG
ISISMDAKGRCFDNIFIERLWRSVKYEYIYLKEFESVAEVREALTDYFEYYNTDRYHQSLEYKTPADVYFSKVKIENIFDNLTSIF
KKMLIEPNNPDIPIVRQCQLLGLNRSVYYYQYKGIDEHTENLMRLIDERYTARPHESSRKIMVYLNNLGYDVTRNQVKYLMDKMGLQAIYPKPITSAKSR
QEHIIYPYLLNDICVYYPNQVWCSDITYIRMKHGHIYLVAIMDWYSRYVIDWQLSISLEAEFCIDTLKRALASSSCGIFNTDQGAQYTSNAWINTLLNKG
ISISMDAKGRCFDNIFIERLWRSVKYEYIYLKEFESVAEVREALTDYFEYYNTDRYHQSLEYKTPADVYFSKVKIENIFDNLTSIF
Blast result :
Comments
ISFop4 is 68% aa similar to ISCysp23.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Elizabeth Dietrich (2018) Direct submission.